SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS30596
         (800 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC926.04c |hsp90|swo1|heat shock protein Hsp90|Schizosaccharom...   156   3e-39
SPBC23G7.06c |||conserved eukaryotic protein|Schizosaccharomyces...    29   0.77 
SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces po...    29   0.77 
SPAC22H12.05c |||fasciclin domain protein |Schizosaccharomyces p...    27   2.3  
SPAC19B12.03 |bgs3||1,3-beta-glucan synthase subunit Bgs3|Schizo...    27   2.3  
SPAC4D7.13 |usp104|prp40|U1 snRNP-associated protein Usp104|Schi...    27   3.1  
SPAC19G12.05 |||mitochondrial citrate transporter|Schizosaccharo...    26   5.4  
SPBC13G1.10c |mug81||ATP-dependent RNA helicase Slh1|Schizosacch...    26   5.4  
SPAC1F3.03 |||Lgl family protein|Schizosaccharomyces pombe|chr 1...    26   5.4  
SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosacc...    26   7.2  
SPAC3A11.08 |pcu4|cul4, Cul-4|cullin 4|Schizosaccharomyces pombe...    26   7.2  
SPAC4A8.05c |myp2|myo3|myosin II heavy chain |Schizosaccharomyce...    26   7.2  

>SPAC926.04c |hsp90|swo1|heat shock protein
           Hsp90|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 704

 Score =  156 bits (379), Expect = 3e-39
 Identities = 73/87 (83%), Positives = 81/87 (93%)
 Frame = +3

Query: 6   LAVKHFSVEGQLEFRALLFVPRRAPFDLFENKKRKNNIKLYVRRVFIMDNCEDLIPEYLN 185
           LAVKHFSVEGQLEFRA+LFVPRRAP DLFE K++KNNIKLYVRRVFI D+CE+LIPE+L 
Sbjct: 299 LAVKHFSVEGQLEFRAILFVPRRAPMDLFEAKRKKNNIKLYVRRVFITDDCEELIPEWLG 358

Query: 186 FIRGVVDSEDLPLNISREMLQQNKF*K 266
           FI+GVVDSEDLPLN+SREMLQQNK  K
Sbjct: 359 FIKGVVDSEDLPLNLSREMLQQNKIMK 385



 Score =  116 bits (279), Expect = 4e-27
 Identities = 50/84 (59%), Positives = 72/84 (85%)
 Frame = +2

Query: 257 ILKVIRKNLVKKCLELFEELAEDKENYKKYYEQFSKNLKLGIHEDSQNRAKLSELLRYHT 436
           I+KVIRKNLV++CL++F E+AEDKEN+K +Y+ FSKNLKLGIHED+ NR  L++LLRY++
Sbjct: 383 IMKVIRKNLVRRCLDMFNEIAEDKENFKTFYDAFSKNLKLGIHEDAANRPALAKLLRYNS 442

Query: 437 SASGDEACSLKEYVSRMKENQKHI 508
             S D+  SL++Y+++M E+QK+I
Sbjct: 443 LNSPDDLISLEDYITKMPEHQKNI 466



 Score = 57.6 bits (133), Expect = 2e-09
 Identities = 22/50 (44%), Positives = 38/50 (76%)
 Frame = +1

Query: 508 HYITGENRDQVANSSFVERVKKRGYEVVYMTEPIDEYVVPQMREYDGKTL 657
           ++ITGE++  V NS F+E  + + ++V++M +PIDEY V Q++E++GK L
Sbjct: 467 YFITGESKQAVENSPFLEIFRAKKFDVLFMVDPIDEYAVTQLKEFEGKKL 516



 Score = 27.5 bits (58), Expect = 2.3
 Identities = 17/35 (48%), Positives = 22/35 (62%), Gaps = 3/35 (8%)
 Frame = +2

Query: 278 NLVKKCLELFEELAEDK---ENYKKYYEQFSKNLK 373
           N+ K  LEL EE  E+K   E  +K YE+F+K LK
Sbjct: 518 NITKDGLEL-EETDEEKAAREKLEKEYEEFAKQLK 551


>SPBC23G7.06c |||conserved eukaryotic protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 745

 Score = 29.1 bits (62), Expect = 0.77
 Identities = 21/71 (29%), Positives = 36/71 (50%), Gaps = 2/71 (2%)
 Frame = +1

Query: 16  NTSPLKVS*SSEHYFLCHVVHLLTCLKTRS--ARTISSCMYEEFSSWTTVRISSQSTLTS 189
           NTSPLK + S+   F+  V   L  LKT++   R+I     +   S TT +  S +  + 
Sbjct: 514 NTSPLKYTHSASKSFIGEVQDSLQALKTKAHKPRSIGGDSSQTTLSETTKKYGSVAKKSF 573

Query: 190 LEVLSTVRTFL 222
            + +S  ++F+
Sbjct: 574 FQGVSDAKSFV 584


>SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 897

 Score = 29.1 bits (62), Expect = 0.77
 Identities = 21/87 (24%), Positives = 37/87 (42%), Gaps = 2/87 (2%)
 Frame = +2

Query: 305 FEELAEDKENYKKYYEQFSKNLKLGI--HEDSQNRAKLSELLRYHTSASGDEACSLKEYV 478
           F++L  D+E  ++  ++++ +          SQN+ +L E        S   + SL  + 
Sbjct: 13  FQQLQNDEELARRLQDEWNSSSPSSAPSSSSSQNQVELDEQFARSLQNSQSSSYSLPPFD 72

Query: 479 SRMKENQKHITTSLEKTETRWRTPHLW 559
               +N    T+S   T  RWR   LW
Sbjct: 73  HPPSKNP---TSSSLSTRKRWRQKRLW 96


>SPAC22H12.05c |||fasciclin domain protein |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 728

 Score = 27.5 bits (58), Expect = 2.3
 Identities = 11/22 (50%), Positives = 16/22 (72%)
 Frame = +1

Query: 457 LLSQRVCIQDEGEPETYHYITG 522
           L++QRV  +D  EP+T+HY  G
Sbjct: 224 LVNQRVYAEDVIEPKTFHYKNG 245


>SPAC19B12.03 |bgs3||1,3-beta-glucan synthase subunit
            Bgs3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1826

 Score = 27.5 bits (58), Expect = 2.3
 Identities = 8/27 (29%), Positives = 16/27 (59%)
 Frame = -2

Query: 619  HIHQWAQSCRLLHSHVSSLSPQMRSSP 539
            H H W   CRL  + ++ ++ +++ SP
Sbjct: 1488 HAHSWIGFCRLARTRITGVNRKVKGSP 1514


>SPAC4D7.13 |usp104|prp40|U1 snRNP-associated protein
           Usp104|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 695

 Score = 27.1 bits (57), Expect = 3.1
 Identities = 18/76 (23%), Positives = 34/76 (44%)
 Frame = +2

Query: 272 RKNLVKKCLELFEELAEDKENYKKYYEQFSKNLKLGIHEDSQNRAKLSELLRYHTSASGD 451
           ++NLV  CLE+ +   +D  N  +   + S+ LK    +  ++ A   +L+    +   D
Sbjct: 473 KRNLVLDCLEVLQISVDDTSNIPEIIARLSEKLK----DREESEAVTEDLIEEVVNRLRD 528

Query: 452 EACSLKEYVSRMKENQ 499
           +A   K    R  E +
Sbjct: 529 KAIHKKAEEKRADERR 544


>SPAC19G12.05 |||mitochondrial citrate
           transporter|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 291

 Score = 26.2 bits (55), Expect = 5.4
 Identities = 13/28 (46%), Positives = 19/28 (67%)
 Frame = +1

Query: 139 FSSWTTVRISSQSTLTSLEVLSTVRTFL 222
           F+++ +++ S QS L   E LSTV TFL
Sbjct: 181 FTAYNSIKQSLQSRLPPDEKLSTVTTFL 208


>SPBC13G1.10c |mug81||ATP-dependent RNA helicase
            Slh1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1935

 Score = 26.2 bits (55), Expect = 5.4
 Identities = 18/67 (26%), Positives = 33/67 (49%)
 Frame = +3

Query: 6    LAVKHFSVEGQLEFRALLFVPRRAPFDLFENKKRKNNIKLYVRRVFIMDNCEDLIPEYLN 185
            LAVK  +  G L    L  +      DL  N+K    +K ++ ++ +++   DL+P   N
Sbjct: 944  LAVKVENQCGSLSLEELSDMSTGELGDLIHNRKMGPTVKKFISKLPLLNINVDLLPLTKN 1003

Query: 186  FIRGVVD 206
             +R V++
Sbjct: 1004 VLRLVLN 1010


>SPAC1F3.03 |||Lgl family protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1004

 Score = 26.2 bits (55), Expect = 5.4
 Identities = 18/68 (26%), Positives = 32/68 (47%)
 Frame = -2

Query: 724 FPHVPSFLPHLQEVLIPSFVTDTGSCHHTPSSVVLHIHQWAQSCRLLHSHVSSLSPQMRS 545
           FP +   +P ++++L+    +   +  H P +++L       S  LL     ++S    S
Sbjct: 370 FPFID--IPPVRDMLVIPSSSPHYNGSHNPKNLLLLSED--NSLSLLDISTGNISNMSLS 425

Query: 544 SPPGLCFL 521
            PP LCFL
Sbjct: 426 IPPSLCFL 433


>SPAC688.08 |srb8|med12|mediator complex subunit Srb8
            |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1233

 Score = 25.8 bits (54), Expect = 7.2
 Identities = 14/41 (34%), Positives = 23/41 (56%), Gaps = 5/41 (12%)
 Frame = +3

Query: 87   LFENKKRKNNIK-----LYVRRVFIMDNCEDLIPEYLNFIR 194
            L EN   KNN+      L  ++  I++ CE +I + LNF++
Sbjct: 1144 LQENTSSKNNLLQKEWILITKKKVILEQCEYVILDILNFLK 1184


>SPAC3A11.08 |pcu4|cul4, Cul-4|cullin 4|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 734

 Score = 25.8 bits (54), Expect = 7.2
 Identities = 15/52 (28%), Positives = 28/52 (53%), Gaps = 1/52 (1%)
 Frame = +2

Query: 275 KNLVKKCLELFEELAEDKENYKKYYE-QFSKNLKLGIHEDSQNRAKLSELLR 427
           K +  + L+LF  +A  K+ ++ YY+   +K L L     +QN   L ++L+
Sbjct: 404 KEVFSEILDLFRYIA-SKDIFEAYYKLDIAKRLLLNKSASAQNELMLLDMLK 454


>SPAC4A8.05c |myp2|myo3|myosin II heavy chain |Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 2104

 Score = 25.8 bits (54), Expect = 7.2
 Identities = 14/56 (25%), Positives = 27/56 (48%), Gaps = 3/56 (5%)
 Frame = +2

Query: 386  EDSQNRAKLSELLRYHTSASGDEACSLKEYVSRMKENQKHITTSLE---KTETRWR 544
            ++ +NR +    L    S   D+   ++  VSR++E    +   LE    +ET+W+
Sbjct: 1994 DECRNRDRTIRQLEAQISKFDDDKKRIQSSVSRLEERNAQLRNQLEDVQASETQWK 2049


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,130,224
Number of Sequences: 5004
Number of extensions: 62546
Number of successful extensions: 204
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 196
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 203
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 388424860
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -