Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS30589
         (783 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC23D3.07 |pup1||20S proteasome component beta 2|Schizosacchar...   125   6e-30
SPBC4C3.10c |||20S proteasome component beta 1|Schizosaccharomyc...    59   8e-10
SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomy...    34   0.026
SPAC4A8.13c |pts1||20S proteasome component beta 5|Schizosacchar...    31   0.25 
SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomy...    29   0.99 
SPBC17D11.06 |spp2|pri2|DNA primase large subunit Spp2 |Schizosa...    27   3.0  
SPAC1039.11c ||SPAC922.02c|alpha-glucosidase|Schizosaccharomyces...    27   4.0  
SPAC4C5.03 |||CTNS domain protein |Schizosaccharomyces pombe|chr...    27   4.0  
SPCC70.07c |tmp1|tmp|thymidylate kinase Tmp1|Schizosaccharomyces...    27   4.0  
SPBC577.10 |||20S proteasome component beta 7|Schizosaccharomyce...    26   5.3  
SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha 3|Sch...    26   7.0  
SPACUNK12.02c |cmk1||calcium/calmodulin-dependent protein kinase...    25   9.3  

>SPAC23D3.07 |pup1||20S proteasome component beta
           2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 267

 Score =  125 bits (302), Expect = 6e-30
 Identities = 69/150 (46%), Positives = 86/150 (57%)
 Frame = +1

Query: 256 KNCQKIHYLASNMYCCGAGTAADTEMTTQSVASQLELQRLHTGRTVPVETAATLLKRMLF 435
           KNC+K+H ++ N++C GAGTAADTE  T  ++S +EL  L+T R   V TA T+LK+ LF
Sbjct: 64  KNCKKLHLISPNIWCAGAGTAADTEFVTSMISSNIELHSLYTNRKPRVVTALTMLKQHLF 123

Query: 436 RYQGHIGAALVLGGVDRTGPHIYCIILMDL*INYHMQPWDLDLLRLWQCLKQVGNVI*MR 615
           RYQGHIGA LVLGG D  GPH++ I                  L     L+        R
Sbjct: 124 RYQGHIGAYLVLGGYDCKGPHLFTIAAHGSSDKLPYVALGSGSLAAISVLETKYQPDLER 183

Query: 616 KKEKSLSEMLFAAGIFNDLGSGSNVDLCVI 705
            +   L +    AGIFNDLGSGSN DL VI
Sbjct: 184 HEAMELVKEAIEAGIFNDLGSGSNCDLVVI 213



 Score = 82.2 bits (194), Expect = 8e-17
 Identities = 36/60 (60%), Positives = 44/60 (73%)
 Frame = +2

Query: 92  GFSFENFQRNAFLAQKGFPAPKATKTGTTIVGIIYADGVILGADTRATENTVVSAKTAKK 271
           GF FE +QRN  L +KGFP PKAT TGTTIVG+I  D ++LGADTRAT   +++ K  KK
Sbjct: 9   GFDFEYYQRNLLLQEKGFPTPKATSTGTTIVGVIAKDCIVLGADTRATAGPIIADKNCKK 68



 Score = 53.2 bits (122), Expect = 4e-08
 Identities = 23/44 (52%), Positives = 32/44 (72%)
 Frame = +3

Query: 516 HGSVDKLPYATMGSGSLAAMAVFEAGWKRDMNEEEGKKLVRDAI 647
           HGS DKLPY  +GSGSLAA++V E  ++ D+   E  +LV++AI
Sbjct: 151 HGSSDKLPYVALGSGSLAAISVLETKYQPDLERHEAMELVKEAI 194


>SPBC4C3.10c |||20S proteasome component beta 1|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 226

 Score = 58.8 bits (136), Expect = 8e-10
 Identities = 29/84 (34%), Positives = 46/84 (54%), Gaps = 1/84 (1%)
 Frame = +1

Query: 268 KIHYLASNMYCCGAGTAADTEMTTQSVASQLELQRLHTGRTVPVETAATLLKRMLFRYQG 447
           K+  L  N++CC +G+AADT+     +   L + R+  G    V TAATL   M ++ + 
Sbjct: 57  KLTQLTDNIWCCRSGSAADTQTVADLLKYYLSMYRIQFGHDPSVHTAATLASEMCYQNKN 116

Query: 448 HIGAALVLGGVD-RTGPHIYCIIL 516
            + A L++ G D +TG  +Y I L
Sbjct: 117 MLSAGLIVAGYDEKTGGDVYSIPL 140



 Score = 35.9 bits (79), Expect = 0.007
 Identities = 16/38 (42%), Positives = 22/38 (57%)
 Frame = +2

Query: 164 KTGTTIVGIIYADGVILGADTRATENTVVSAKTAKKST 277
           + GTTI  + Y DGVIL AD+R T    ++ +   K T
Sbjct: 22  RMGTTITALRYKDGVILAADSRTTMGAYIANRVTDKLT 59



 Score = 31.5 bits (68), Expect = 0.14
 Identities = 14/43 (32%), Positives = 25/43 (58%)
 Frame = +3

Query: 519 GSVDKLPYATMGSGSLAAMAVFEAGWKRDMNEEEGKKLVRDAI 647
           GS+ K P A  GSGS       +A ++ +M +EE  + +++A+
Sbjct: 142 GSLHKQPLAIGGSGSAFIYGFCDANFRENMTQEEAVEFLKNAV 184


>SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 245

 Score = 33.9 bits (74), Expect = 0.026
 Identities = 20/58 (34%), Positives = 25/58 (43%)
 Frame = +2

Query: 95  FSFENFQRNAFLAQKGFPAPKATKTGTTIVGIIYADGVILGADTRATENTVVSAKTAK 268
           FS   F  N  L Q  + A  A   G T VGI   DGV+L  + + T    + A   K
Sbjct: 7   FSLTTFSPNGKLVQIEY-ALNAVNAGVTSVGIKATDGVVLATEKKPTSELAIGASLEK 63


>SPAC4A8.13c |pts1||20S proteasome component beta
           5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 272

 Score = 30.7 bits (66), Expect = 0.25
 Identities = 18/64 (28%), Positives = 31/64 (48%)
 Frame = +2

Query: 80  IPVPGFSFENFQRNAFLAQKGFPAPKATKTGTTIVGIIYADGVILGADTRATENTVVSAK 259
           +PVP  S   + RN     K          GTT +   Y  G+++  D+RA+   +++++
Sbjct: 33  VPVPQSSL--YLRNLTDETKNKHCLIKMNHGTTTLAFRYQHGIVVCVDSRASAGPLIASQ 90

Query: 260 TAKK 271
           T KK
Sbjct: 91  TVKK 94


>SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 247

 Score = 28.7 bits (61), Expect = 0.99
 Identities = 14/40 (35%), Positives = 21/40 (52%)
 Frame = +2

Query: 149 APKATKTGTTIVGIIYADGVILGADTRATENTVVSAKTAK 268
           A +A K G+T +G+   D V+LG + R T   + S    K
Sbjct: 27  AIEAIKLGSTAIGVKTKDAVVLGVEKRLTSPLMESHSVEK 66



 Score = 26.2 bits (55), Expect = 5.3
 Identities = 19/91 (20%), Positives = 37/91 (40%), Gaps = 11/91 (12%)
 Frame = +1

Query: 265 QKIHYLASNMYCCGAGTAADTEMTTQSVASQLELQRLHTGRTVPVETAATLLKRMLFRY- 441
           +K+  + S++ C  +G  AD     +    Q +  R        +E+    +  +  R+ 
Sbjct: 65  EKLFEIDSHIGCAISGLTADARTIIEHARVQTQNHRFTYDEPQGIESTTQSICDLALRFG 124

Query: 442 QGH----------IGAALVLGGVDRTGPHIY 504
           +G            G AL++ G+D  GP +Y
Sbjct: 125 EGEDGEERIMSRPFGVALLIAGIDEHGPQLY 155


>SPBC17D11.06 |spp2|pri2|DNA primase large subunit Spp2
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 459

 Score = 27.1 bits (57), Expect = 3.0
 Identities = 16/56 (28%), Positives = 25/56 (44%)
 Frame = -2

Query: 716 PVLRITHRSTLEPEPKSLNIPAANSISDKLFSFFLIHITFPTCFKHCHSRKRSRSH 549
           P+L    +  + PE  S+  P + +I+      F+ H  FP C KH H   +   H
Sbjct: 252 PILNHLSKGFVAPE-SSIVAPKSGAITAGQVDSFVSH--FPLCAKHLHEVLKRDKH 304


>SPAC1039.11c ||SPAC922.02c|alpha-glucosidase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 995

 Score = 26.6 bits (56), Expect = 4.0
 Identities = 13/45 (28%), Positives = 23/45 (51%)
 Frame = -1

Query: 534 IYLQIHEDNAVNMRTGSVNTSQNQSSPYMALIAK*HSFQQCGSSF 400
           I + I   N + M+  S+ T + + +PY  L+A     + CGS +
Sbjct: 853 INVAIRGGNIIPMQKPSLTTHETKQNPYDLLVALDSDRKACGSLY 897


>SPAC4C5.03 |||CTNS domain protein |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 302

 Score = 26.6 bits (56), Expect = 4.0
 Identities = 15/62 (24%), Positives = 25/62 (40%)
 Frame = -2

Query: 422 FSSVAAVSTGTVRPVCKRCNSNCEATDCXXXXXXXXXXXPQQYMLDAK*WIFWQFLPRQL 243
           FS+V A+    + P+ + C +      C            Q  ++      FW FLPR +
Sbjct: 72  FSNVIALQ---ISPLTECCQNYYSKKQCFANTTGLIQVGSQVLIMGCVLMAFWMFLPRPI 128

Query: 242 YF 237
           +F
Sbjct: 129 HF 130


>SPCC70.07c |tmp1|tmp|thymidylate kinase Tmp1|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 210

 Score = 26.6 bits (56), Expect = 4.0
 Identities = 11/29 (37%), Positives = 18/29 (62%)
 Frame = +1

Query: 454 GAALVLGGVDRTGPHIYCIILMDL*INYH 540
           G  +V+ G+DR+G    C +L+D  I+ H
Sbjct: 7   GRLIVIEGLDRSGKSTQCQLLVDKLISQH 35


>SPBC577.10 |||20S proteasome component beta 7|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 262

 Score = 26.2 bits (55), Expect = 5.3
 Identities = 9/23 (39%), Positives = 18/23 (78%)
 Frame = +2

Query: 167 TGTTIVGIIYADGVILGADTRAT 235
           TG++++ + +ADGV++ AD  A+
Sbjct: 42  TGSSVLALKFADGVMIAADNLAS 64


>SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha
           3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 248

 Score = 25.8 bits (54), Expect = 7.0
 Identities = 9/30 (30%), Positives = 19/30 (63%)
 Frame = +2

Query: 182 VGIIYADGVILGADTRATENTVVSAKTAKK 271
           +GI+  DG++L A+ + T   +   ++A+K
Sbjct: 35  LGIVAKDGIVLAAEKKVTSKLLEQEESAEK 64


>SPACUNK12.02c |cmk1||calcium/calmodulin-dependent protein kinase
           Cmk1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 335

 Score = 25.4 bits (53), Expect = 9.3
 Identities = 15/40 (37%), Positives = 19/40 (47%)
 Frame = +2

Query: 149 APKATKTGTTIVGIIYADGVILGADTRATENTVVSAKTAK 268
           APKAT     ++   Y  G +LG  T AT    V  +T K
Sbjct: 16  APKATVDQKQLLPCKYRVGRVLGGGTYATVREAVHIETNK 55


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,213,430
Number of Sequences: 5004
Number of extensions: 67422
Number of successful extensions: 183
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 172
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 183
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 379359666
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)