Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30587
(827 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_Q2F5L0 Cluster: Signal sequence receptor; n=2; Endopter... 214 2e-54
UniRef50_Q9W335 Cluster: CG32701-PA; n=5; Endopterygota|Rep: CG3... 132 1e-29
UniRef50_P43307 Cluster: Translocon-associated protein subunit a... 112 9e-24
UniRef50_A7RRI8 Cluster: Predicted protein; n=1; Nematostella ve... 101 2e-20
UniRef50_Q95XT5 Cluster: Putative uncharacterized protein; n=2; ... 96 1e-18
UniRef50_UPI0000E46DC1 Cluster: PREDICTED: hypothetical protein;... 87 7e-16
UniRef50_Q4T9K6 Cluster: Chromosome undetermined SCAF7543, whole... 52 1e-05
UniRef50_Q54R45 Cluster: Putative uncharacterized protein; n=1; ... 52 2e-05
UniRef50_Q86FE2 Cluster: Clone ZZD1494 mRNA sequence; n=2; Schis... 45 0.003
UniRef50_A4RX78 Cluster: Predicted protein; n=2; Ostreococcus|Re... 44 0.006
UniRef50_Q4T5B3 Cluster: Chromosome undetermined SCAF9335, whole... 38 0.23
UniRef50_P45434 Cluster: Translocon-associated protein subunit a... 38 0.41
>UniRef50_Q2F5L0 Cluster: Signal sequence receptor; n=2;
Endopterygota|Rep: Signal sequence receptor - Bombyx
mori (Silk moth)
Length = 279
Score = 214 bits (523), Expect = 2e-54
Identities = 100/104 (96%), Positives = 100/104 (96%)
Frame = +3
Query: 255 KSSQDIDTTILFTKPVPSLGDLTFDIQAGYPVEFFVGFINKGSVDYVVESMEASFRYPMD 434
KSSQDIDTTILFTKPVPSLGDLTFDIQAGYPVEFFVGFINKGSVDYVVESMEASFRYPMD
Sbjct: 60 KSSQDIDTTILFTKPVPSLGDLTFDIQAGYPVEFFVGFINKGSVDYVVESMEASFRYPMD 119
Query: 435 YTYYIQNFTALPYNREVKPKQEATLPIPFIPNEAFAGRPFGLNV 566
YTYYIQNFTALPYNREVKPKQEAT FIPNEAFAGRPFGLNV
Sbjct: 120 YTYYIQNFTALPYNREVKPKQEATFAYSFIPNEAFAGRPFGLNV 163
Score = 85.4 bits (202), Expect = 2e-15
Identities = 44/59 (74%), Positives = 44/59 (74%)
Frame = +1
Query: 79 MKLIYFTFLIVPAALLCLGSGNILARAXXXXXXXXXXXXXXXNQVVGDDALVDDDESVV 255
MKLIYFTFLIVPAALLCLGSGNILARA NQVVGDDALVDDDESVV
Sbjct: 1 MKLIYFTFLIVPAALLCLGSGNILARAEEDELDDVVDIEGEDNQVVGDDALVDDDESVV 59
Score = 43.2 bits (97), Expect = 0.008
Identities = 25/35 (71%), Positives = 25/35 (71%)
Frame = +1
Query: 574 NYRDASGNAYQEAVYNQTPGNIVGKLSGRSKMGET 678
NYRDASGNAYQEAVYNQT NIV G GET
Sbjct: 166 NYRDASGNAYQEAVYNQTL-NIVEVSEGLD--GET 197
>UniRef50_Q9W335 Cluster: CG32701-PA; n=5; Endopterygota|Rep:
CG32701-PA - Drosophila melanogaster (Fruit fly)
Length = 302
Score = 132 bits (318), Expect = 1e-29
Identities = 58/103 (56%), Positives = 80/103 (77%)
Frame = +3
Query: 258 SSQDIDTTILFTKPVPSLGDLTFDIQAGYPVEFFVGFINKGSVDYVVESMEASFRYPMDY 437
SS + DT +LFTKP+ + G D+ G PVEF +GF NKG+ ++V+E++EASFRYPMD+
Sbjct: 64 SSPNADTYLLFTKPLYTPGQ-QLDLPGGKPVEFLIGFTNKGAEEFVIETVEASFRYPMDF 122
Query: 438 TYYIQNFTALPYNREVKPKQEATLPIPFIPNEAFAGRPFGLNV 566
Y+IQNF+A+ YNREVKP E+T+ F+P++ FAGRPFGLN+
Sbjct: 123 NYFIQNFSAVAYNREVKPGFESTVSYTFLPSDQFAGRPFGLNI 165
>UniRef50_P43307 Cluster: Translocon-associated protein subunit
alpha precursor; n=51; Euteleostomi|Rep:
Translocon-associated protein subunit alpha precursor -
Homo sapiens (Human)
Length = 286
Score = 112 bits (270), Expect = 9e-24
Identities = 71/162 (43%), Positives = 90/162 (55%)
Frame = +3
Query: 255 KSSQDIDTTILFTKPVPSLGDLTFDIQAGYPVEFFVGFINKGSVDYVVESMEASFRYPMD 434
++S DTTILF K G+ D A V+F VGF NKG+ D++VES++ASFRYP D
Sbjct: 78 EASPSADTTILFVK-----GE---DFPANNIVKFLVGFTNKGTEDFIVESLDASFRYPQD 129
Query: 435 YTYYIQNFTALPYNREVKPKQEATLPIPFIPNEAFAGRPFGLNVXXXXXXXXXKCLPGSR 614
Y +YIQNFTALP N V P+++AT FIP E GRPFGL + K L G+
Sbjct: 130 YQFYIQNFTALPLNTVVPPQRQATFEYSFIPAEPMGGRPFGLVI-----NLNYKDLNGNV 184
Query: 615 LQPDTWKHCGKVIWKV*DGRDHFLLIRFFPLGGLGWEVIWAL 740
Q + VI + DG D + + L GLG VI L
Sbjct: 185 FQDAVFNQTVTVIERE-DGLDGETIFMYMFLAGLGLLVIVGL 225
>UniRef50_A7RRI8 Cluster: Predicted protein; n=1; Nematostella
vectensis|Rep: Predicted protein - Nematostella
vectensis
Length = 291
Score = 101 bits (242), Expect = 2e-20
Identities = 51/107 (47%), Positives = 63/107 (58%)
Frame = +3
Query: 246 ECCKSSQDIDTTILFTKPVPSLGDLTFDIQAGYPVEFFVGFINKGSVDYVVESMEASFRY 425
+ K S D DT ILFTK AG + V F N G D++V+ MEAS RY
Sbjct: 78 DALKPSPDADTVILFTKNTEQ------QFSAGLVSKCLVSFSNNGRNDFMVDMMEASLRY 131
Query: 426 PMDYTYYIQNFTALPYNREVKPKQEATLPIPFIPNEAFAGRPFGLNV 566
P DY+YYIQNFTA YN VKP +A+ F P+E+F GRPFGL++
Sbjct: 132 PQDYSYYIQNFTAFQYNTVVKPGHQASFEYAFRPHESFGGRPFGLSI 178
>UniRef50_Q95XT5 Cluster: Putative uncharacterized protein; n=2;
Caenorhabditis|Rep: Putative uncharacterized protein -
Caenorhabditis elegans
Length = 257
Score = 95.9 bits (228), Expect = 1e-18
Identities = 45/98 (45%), Positives = 61/98 (62%)
Frame = +3
Query: 273 DTTILFTKPVPSLGDLTFDIQAGYPVEFFVGFINKGSVDYVVESMEASFRYPMDYTYYIQ 452
D + F PS ++ + G PV++ +GF NKG D+VV+ E SFR+P D++Y++Q
Sbjct: 48 DAGLAFHFVQPSDANVVREFYTGKPVKYLIGFQNKGEKDFVVKYAETSFRFPTDHSYHLQ 107
Query: 453 NFTALPYNREVKPKQEATLPIPFIPNEAFAGRPFGLNV 566
NFT YNR V PK+E TL F +E FAGRP GL V
Sbjct: 108 NFTRGEYNRRVAPKEEVTLDYGFYAHETFAGRPVGLVV 145
>UniRef50_UPI0000E46DC1 Cluster: PREDICTED: hypothetical protein;
n=1; Strongylocentrotus purpuratus|Rep: PREDICTED:
hypothetical protein - Strongylocentrotus purpuratus
Length = 247
Score = 86.6 bits (205), Expect = 7e-16
Identities = 44/101 (43%), Positives = 59/101 (58%)
Frame = +3
Query: 255 KSSQDIDTTILFTKPVPSLGDLTFDIQAGYPVEFFVGFINKGSVDYVVESMEASFRYPMD 434
K+S D DTT LFT + G+ +I P++ VGF N G D+ +ES+ A+ RYP D
Sbjct: 89 KASPDADTTFLFT----TNGEK--EIPVTIPLQVLVGFTNNGQQDFTIESLHATLRYPQD 142
Query: 435 YTYYIQNFTALPYNREVKPKQEATLPIPFIPNEAFAGRPFG 557
+++YI NFT YN V+P +EAT F E FA R FG
Sbjct: 143 FSFYIYNFTEKEYNTVVEPNKEATFEYSFQAGEVFAARQFG 183
>UniRef50_Q4T9K6 Cluster: Chromosome undetermined SCAF7543, whole
genome shotgun sequence; n=1; Tetraodon
nigroviridis|Rep: Chromosome undetermined SCAF7543,
whole genome shotgun sequence - Tetraodon nigroviridis
(Green puffer)
Length = 316
Score = 52.4 bits (120), Expect = 1e-05
Identities = 30/58 (51%), Positives = 38/58 (65%)
Frame = +3
Query: 255 KSSQDIDTTILFTKPVPSLGDLTFDIQAGYPVEFFVGFINKGSVDYVVESMEASFRYP 428
K+S DTTILF K G+ D A V+F +GF NKGS ++VVES++ASFRYP
Sbjct: 85 KASPHADTTILFVK-----GE---DFPANNIVKFLLGFTNKGSENFVVESLDASFRYP 134
Score = 33.9 bits (74), Expect = 5.0
Identities = 17/38 (44%), Positives = 25/38 (65%)
Frame = +1
Query: 568 SFNYRDASGNAYQEAVYNQTPGNIVGKLSGRSKMGETI 681
+ NY+D++GN +Q+AV+NQT + K G GETI
Sbjct: 205 NLNYKDSAGNVFQDAVFNQTV-TVTEKEDGLD--GETI 239
>UniRef50_Q54R45 Cluster: Putative uncharacterized protein; n=1;
Dictyostelium discoideum AX4|Rep: Putative
uncharacterized protein - Dictyostelium discoideum AX4
Length = 236
Score = 52.0 bits (119), Expect = 2e-05
Identities = 27/75 (36%), Positives = 38/75 (50%)
Frame = +3
Query: 336 AGYPVEFFVGFINKGSVDYVVESMEASFRYPMDYTYYIQNFTALPYNREVKPKQEATLPI 515
AG VE VGF N + + AS +P D++ +IQN+T + V+ ATL
Sbjct: 55 AGSVVEVLVGFTNNADKALNITHIFASLNHPQDFSVFIQNYTRGDFGIAVEKGHHATLAY 114
Query: 516 PFIPNEAFAGRPFGL 560
F+P+E R FGL
Sbjct: 115 RFVPSEYLEPREFGL 129
>UniRef50_Q86FE2 Cluster: Clone ZZD1494 mRNA sequence; n=2;
Schistosoma japonicum|Rep: Clone ZZD1494 mRNA sequence -
Schistosoma japonicum (Blood fluke)
Length = 269
Score = 44.8 bits (101), Expect = 0.003
Identities = 30/109 (27%), Positives = 47/109 (43%), Gaps = 5/109 (4%)
Frame = +3
Query: 255 KSSQDIDTTILFTKPVPSLGDLTFDIQ--AGYPVEFFVGFINKGSVD---YVVESMEASF 419
K S + + F+KP L ++ AG N D + ++ +E +
Sbjct: 50 KGSPAVSVLVAFSKPAYGLYSARREMNFPAGEVSSLVASLANTNPPDKEQFRLDFIEGAL 109
Query: 420 RYPMDYTYYIQNFTALPYNREVKPKQEATLPIPFIPNEAFAGRPFGLNV 566
YP Y Y +QNFT ++P QE +L F P+ AGR F L++
Sbjct: 110 HYPSYYDYRMQNFTRQRLQGTLEPGQEISLYYRFKPSPELAGRSFDLSI 158
>UniRef50_A4RX78 Cluster: Predicted protein; n=2; Ostreococcus|Rep:
Predicted protein - Ostreococcus lucimarinus CCE9901
Length = 249
Score = 43.6 bits (98), Expect = 0.006
Identities = 28/102 (27%), Positives = 47/102 (46%), Gaps = 1/102 (0%)
Frame = +3
Query: 258 SSQDIDTTILFTKPVPSLGDLTFDIQAGYPVEFFVGFINKGSVDYVVESMEASFRYPMDY 437
++ D+++ + F P D F G V+ +G N G+ V + S P ++
Sbjct: 35 ANPDVESVVAF----PDHADRAFP--GGERVKVLLGLRNTGNDAVNVTHLAGSLNVPNNF 88
Query: 438 TYYIQNFTALPY-NREVKPKQEATLPIPFIPNEAFAGRPFGL 560
+Y+ NFT + + +KPK+EAT F + FAG L
Sbjct: 89 NFYVTNFTVSSFGDAVIKPKKEATFEYEFAIDPQFAGHSLQL 130
>UniRef50_Q4T5B3 Cluster: Chromosome undetermined SCAF9335, whole
genome shotgun sequence; n=8; Tetraodon
nigroviridis|Rep: Chromosome undetermined SCAF9335,
whole genome shotgun sequence - Tetraodon nigroviridis
(Green puffer)
Length = 219
Score = 38.3 bits (85), Expect = 0.23
Identities = 18/36 (50%), Positives = 20/36 (55%)
Frame = +3
Query: 453 NFTALPYNREVKPKQEATLPIPFIPNEAFAGRPFGL 560
NFTAL V P ++AT FIP E G PFGL
Sbjct: 1 NFTALQLGTVVPPARQATFECSFIPAEPMGGAPFGL 36
>UniRef50_P45434 Cluster: Translocon-associated protein subunit
alpha precursor; n=9; Magnoliophyta|Rep:
Translocon-associated protein subunit alpha precursor -
Arabidopsis thaliana (Mouse-ear cress)
Length = 258
Score = 37.5 bits (83), Expect = 0.41
Identities = 24/78 (30%), Positives = 32/78 (41%), Gaps = 1/78 (1%)
Frame = +3
Query: 330 IQAGYPVEFFVGFINKGSVDYVVESMEASFRYPMDYTYYIQNFTALPYNREVKPKQ-EAT 506
+ AG E VG N+G V + AS P D+ +QN T L N P +AT
Sbjct: 78 VPAGEETELLVGLKNEGKTRVGVMGIRASVHLPYDHKLLVQNLTMLRLNNASIPTSLQAT 137
Query: 507 LPIPFIPNEAFAGRPFGL 560
P F ++ F L
Sbjct: 138 FPYIFAVSQYLQPGAFDL 155
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 859,352,420
Number of Sequences: 1657284
Number of extensions: 18163014
Number of successful extensions: 44207
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 42460
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 44193
length of database: 575,637,011
effective HSP length: 100
effective length of database: 409,908,611
effective search space used: 71734006925
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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