BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30586 (817 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 146 3e-36 SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 145 8e-36 SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 130 2e-31 SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 99 4e-22 SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 87 3e-18 SPAC3F10.16c |||GTP binding protein, HSR1-related|Schizosaccharo... 32 0.085 SPBC16C6.05 |||translation initiation factor |Schizosaccharomyce... 30 0.34 SPAC26F1.01 |sec74|SPAPJ691.01c|guanyl-nucleotide exchange facto... 29 0.79 SPAC23D3.01 |||PWWP domain protein|Schizosaccharomyces pombe|chr... 28 1.8 SPBC3B9.02c |cwf28||splicing factor Cwf28|Schizosaccharomyces po... 28 1.8 SPAC2C4.15c |ubx2|ucp13|UBX domain protein Ubx2|Schizosaccharomy... 27 4.2 SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 27 4.2 SPAC31A2.09c |apm4||AP-2 adaptor complex subunit Apm4 |Schizosac... 26 7.4 SPBC16A3.11 |eso1||sister chromatid cohesion protein Eso1|Schizo... 26 7.4 SPBC3H7.02 |||sulfate transporter |Schizosaccharomyces pombe|chr... 25 9.7 SPBC1734.01c ||SPBC337.17c|RNA-binding protein|Schizosaccharomyc... 25 9.7 SPAPB18E9.02c |ppk18||serine/threonine protein kinase Ppk18 |Sch... 25 9.7 >SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces pombe|chr 1|||Manual Length = 644 Score = 146 bits (354), Expect = 3e-36 Identities = 71/84 (84%), Positives = 76/84 (90%) Frame = +1 Query: 256 LIQVFEVERAMTKDNNLLGKFELTGIPPAPRGVPQIEVTFDIDANGILNVSAIEKSINKE 435 LIQVFE ERA TKD NLLGKFEL+GIPPAPRGVPQIEVTFD+DANGILNVSA+EK K Sbjct: 437 LIQVFEGERARTKDCNLLGKFELSGIPPAPRGVPQIEVTFDVDANGILNVSALEKGTGKT 496 Query: 436 NKITITNDKGRLSKEEIERMVNEA 507 KITITNDKGRLSKEEI+RMV+EA Sbjct: 497 QKITITNDKGRLSKEEIDRMVSEA 520 Score = 73.3 bits (172), Expect = 4e-14 Identities = 41/84 (48%), Positives = 43/84 (51%) Frame = +2 Query: 2 FNGKELNKSINPDEXXXXXXXXXXXILHGDKSEEVQDXXXXXXXXXXXGIETAGGVMTTL 181 FNGKE KSINPDE IL GD SE+ QD GIETAGGVMT L Sbjct: 352 FNGKEPCKSINPDEAVAYGAAVQAAILVGDTSEKTQDLLLLDVAPLSLGIETAGGVMTPL 411 Query: 182 IKRNXXXXXXXXXXXXXYSDNQPG 253 IKRN Y+DNQPG Sbjct: 412 IKRNTTIPTKKSEVFSTYADNQPG 435 Score = 44.8 bits (101), Expect = 1e-05 Identities = 18/38 (47%), Positives = 28/38 (73%) Frame = +3 Query: 510 KYRNEDDKQKETIQAKNALESYCFSMKSTMEDEKLKGK 623 KY+ ED+ + IQAKN LESY +S++++++D LK K Sbjct: 522 KYKAEDEAETSRIQAKNHLESYAYSLRNSLDDPNLKDK 559 >SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces pombe|chr 3|||Manual Length = 647 Score = 145 bits (351), Expect = 8e-36 Identities = 71/84 (84%), Positives = 75/84 (89%) Frame = +1 Query: 256 LIQVFEVERAMTKDNNLLGKFELTGIPPAPRGVPQIEVTFDIDANGILNVSAIEKSINKE 435 LIQVFE ERA TKD NLLGKFEL+GIPPAPRGVPQIEVTFD+DANGILNVSA+EK K Sbjct: 437 LIQVFEGERARTKDCNLLGKFELSGIPPAPRGVPQIEVTFDVDANGILNVSALEKGTGKT 496 Query: 436 NKITITNDKGRLSKEEIERMVNEA 507 KITITNDKGRLSKEEI+RMV EA Sbjct: 497 QKITITNDKGRLSKEEIDRMVAEA 520 Score = 74.5 bits (175), Expect = 2e-14 Identities = 41/84 (48%), Positives = 43/84 (51%) Frame = +2 Query: 2 FNGKELNKSINPDEXXXXXXXXXXXILHGDKSEEVQDXXXXXXXXXXXGIETAGGVMTTL 181 FNGKE KSINPDE +L GD SE+ QD GIETAGGVMT L Sbjct: 352 FNGKEPCKSINPDEAVAYGAAVQAAVLTGDTSEKTQDLLLLDVAPLSMGIETAGGVMTPL 411 Query: 182 IKRNXXXXXXXXXXXXXYSDNQPG 253 IKRN YSDNQPG Sbjct: 412 IKRNTTIPTKKSEIFSTYSDNQPG 435 Score = 44.4 bits (100), Expect = 2e-05 Identities = 18/38 (47%), Positives = 28/38 (73%) Frame = +3 Query: 510 KYRNEDDKQKETIQAKNALESYCFSMKSTMEDEKLKGK 623 KY+ ED+ + IQAKN LESY +S++++++D LK K Sbjct: 522 KYKAEDEAESGRIQAKNHLESYAYSLRNSLDDPNLKDK 559 >SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||Manual Length = 663 Score = 130 bits (314), Expect = 2e-31 Identities = 62/85 (72%), Positives = 73/85 (85%), Gaps = 1/85 (1%) Frame = +1 Query: 256 LIQVFEVERAMTKDNNLLGKFELTGIPPAPRGVPQIEVTFDIDANGILNVSAIEKS-INK 432 LIQV+E ER +TKDNNLLGKF+L GIPPAPRGVPQIEVTF++DANG+L VSA++KS K Sbjct: 466 LIQVYEGERTLTKDNNLLGKFDLRGIPPAPRGVPQIEVTFEVDANGVLTVSAVDKSGKGK 525 Query: 433 ENKITITNDKGRLSKEEIERMVNEA 507 K+ I NDKGRLS+E+IERMV EA Sbjct: 526 PEKLVIKNDKGRLSEEDIERMVKEA 550 Score = 41.5 bits (93), Expect = 1e-04 Identities = 24/64 (37%), Positives = 31/64 (48%) Frame = +2 Query: 2 FNGKELNKSINPDEXXXXXXXXXXXILHGDKSEEVQDXXXXXXXXXXXGIETAGGVMTTL 181 F GK+ +K INPDE +L G++ + + GIET GGVMT L Sbjct: 383 FFGKKASKGINPDEAVAYGAAVQAGVLSGEEGSD--NIVLLDVIPLTLGIETTGGVMTKL 440 Query: 182 IKRN 193 I RN Sbjct: 441 IGRN 444 Score = 36.7 bits (81), Expect = 0.004 Identities = 17/39 (43%), Positives = 26/39 (66%), Gaps = 1/39 (2%) Frame = +3 Query: 510 KYRNEDDKQKETIQAKNALESYCFSMKSTM-EDEKLKGK 623 ++ ED KE I+A+N LE+Y +S+K +DE+L GK Sbjct: 552 EFAEEDKILKERIEARNTLENYAYSLKGQFDDDEQLGGK 590 >SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces pombe|chr 1|||Manual Length = 674 Score = 99 bits (238), Expect = 4e-22 Identities = 46/83 (55%), Positives = 64/83 (77%) Frame = +1 Query: 259 IQVFEVERAMTKDNNLLGKFELTGIPPAPRGVPQIEVTFDIDANGILNVSAIEKSINKEN 438 I+VF+ ER + +DN L+G F+LTGI PAP+G PQIEV+FD+DA+GI+NVSA +K+ NK++ Sbjct: 481 IRVFQGERELVRDNKLIGNFQLTGIAPAPKGQPQIEVSFDVDADGIINVSARDKATNKDS 540 Query: 439 KITITNDKGRLSKEEIERMVNEA 507 IT+ G L+ EIE MV +A Sbjct: 541 SITVAGSSG-LTDSEIEAMVADA 562 Score = 36.3 bits (80), Expect = 0.005 Identities = 23/61 (37%), Positives = 27/61 (44%) Frame = +2 Query: 11 KELNKSINPDEXXXXXXXXXXXILHGDKSEEVQDXXXXXXXXXXXGIETAGGVMTTLIKR 190 +E KS+NPDE +L G V+D GIET GGV T LI R Sbjct: 402 REPAKSVNPDEAVAIGAAIQGGVLSG----HVKDLVLLDVTPLSLGIETLGGVFTRLINR 457 Query: 191 N 193 N Sbjct: 458 N 458 Score = 29.1 bits (62), Expect = 0.79 Identities = 14/36 (38%), Positives = 20/36 (55%) Frame = +3 Query: 510 KYRNEDDKQKETIQAKNALESYCFSMKSTMEDEKLK 617 KYR D +KE I+ N ES C ++S ++ K K Sbjct: 564 KYRASDMARKEAIENGNRAESVCTDIESNLDIHKDK 599 >SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces pombe|chr 2|||Manual Length = 613 Score = 87.0 bits (206), Expect = 3e-18 Identities = 39/81 (48%), Positives = 55/81 (67%) Frame = +1 Query: 265 VFEVERAMTKDNNLLGKFELTGIPPAPRGVPQIEVTFDIDANGILNVSAIEKSINKENKI 444 V++ ER +N LG+F+LTGIPP PRG ++E TF++DANGIL V+A+EK+ + I Sbjct: 445 VYQGERVNCAENEPLGEFQLTGIPPMPRGQAELEATFELDANGILKVTAVEKTTGRSAHI 504 Query: 445 TITNDKGRLSKEEIERMVNEA 507 ITN G LS +I+ M+ A Sbjct: 505 EITNSVGHLSSTKIQEMIENA 525 Score = 35.1 bits (77), Expect = 0.012 Identities = 23/83 (27%), Positives = 32/83 (38%), Gaps = 1/83 (1%) Frame = +2 Query: 2 FNGKELNKSINPDEXXXXXXXXXXXIL-HGDKSEEVQDXXXXXXXXXXXGIETAGGVMTT 178 F+G+ LNKSINPDE +L + S++ QD G+ G V Sbjct: 356 FDGRALNKSINPDEAVAYGAAVQAAVLTNKADSDKTQDLLLLDVVPLSLGVAMEGNVFGV 415 Query: 179 LIKRNXXXXXXXXXXXXXYSDNQ 247 + RN +DNQ Sbjct: 416 VCPRNTPIPTIKKRTFTTVADNQ 438 >SPAC3F10.16c |||GTP binding protein, HSR1-related|Schizosaccharomyces pombe|chr 1|||Manual Length = 616 Score = 32.3 bits (70), Expect = 0.085 Identities = 28/89 (31%), Positives = 39/89 (43%), Gaps = 6/89 (6%) Frame = +1 Query: 367 VTFDIDANGILNVSA---IEKSINKENKITITNDKGR---LSKEEIERMVNEAGSTETRM 528 VT + D + LN + +E K+N I N + LSKEE R + + R+ Sbjct: 49 VTHETDLDEFLNTAELGEVEFIAEKQNVTVIQNPEQNPFLLSKEEAARSKQKQEKNKDRL 108 Query: 529 TSKRRPSRPRMHWNLTASA*SLPWRMRSS 615 T RRP HW+ T +A L R S Sbjct: 109 TIPRRP-----HWDQTTTAVELDRMERES 132 >SPBC16C6.05 |||translation initiation factor |Schizosaccharomyces pombe|chr 2|||Manual Length = 190 Score = 30.3 bits (65), Expect = 0.34 Identities = 15/54 (27%), Positives = 27/54 (50%) Frame = +1 Query: 382 DANGILNVSAIEKSINKENKITITNDKGRLSKEEIERMVNEAGSTETRMTSKRR 543 D LNVS + S +E +T ++ R+ +EE +RM ++ T ++R Sbjct: 58 DLENTLNVSGTKDSNAEEQPAKLTKEEKRVEREEAKRMASKVLIKTIERTKRKR 111 >SPAC26F1.01 |sec74|SPAPJ691.01c|guanyl-nucleotide exchange factor Sec74|Schizosaccharomyces pombe|chr 1|||Manual Length = 928 Score = 29.1 bits (62), Expect = 0.79 Identities = 25/76 (32%), Positives = 36/76 (47%), Gaps = 5/76 (6%) Frame = +1 Query: 406 SAIEKSINKEN---KITITNDKGRLSKEEIERMVNEAGSTETRMTSKRRPSRPRMHWNLT 576 SA+EK K K+TIT+D ++S + G + T + SK S + N Sbjct: 41 SALEKKNRKSKSKPKVTITSDTPKVSSQHSPVSSAYTGDSTTDLDSKSGHSSSQKLSNKV 100 Query: 577 ASA*SL--PWRMRSSR 618 +SA L P R RSS+ Sbjct: 101 SSALKLTIPKRWRSSK 116 >SPAC23D3.01 |||PWWP domain protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 407 Score = 27.9 bits (59), Expect = 1.8 Identities = 12/22 (54%), Positives = 15/22 (68%) Frame = +1 Query: 487 ERMVNEAGSTETRMTSKRRPSR 552 +R+ NE S E TSK+RPSR Sbjct: 200 KRLKNEKSSAEISQTSKQRPSR 221 >SPBC3B9.02c |cwf28||splicing factor Cwf28|Schizosaccharomyces pombe|chr 2|||Manual Length = 381 Score = 27.9 bits (59), Expect = 1.8 Identities = 16/52 (30%), Positives = 25/52 (48%), Gaps = 1/52 (1%) Frame = +3 Query: 390 RYPQRFRYREVHQQGEQDHHYQRQRSS-LQGRDRAYG**GRKYRNEDDKQKE 542 R P R +YRE H + + H R QGR +Y R +R++ ++E Sbjct: 320 RDPDRTKYREYHSERRKQHRTDRYSDDYYQGRSYSYK--KRSHRSDRYTERE 369 >SPAC2C4.15c |ubx2|ucp13|UBX domain protein Ubx2|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 26.6 bits (56), Expect = 4.2 Identities = 15/27 (55%), Positives = 15/27 (55%) Frame = -3 Query: 407 ETLRIPLASMSKVTSI*GTPRGAGGIP 327 E L PLA MS TSI G G GG P Sbjct: 76 EVLVDPLADMSAGTSIMGNNFGFGGFP 102 >SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Schizosaccharomyces pombe|chr 1|||Manual Length = 720 Score = 26.6 bits (56), Expect = 4.2 Identities = 9/22 (40%), Positives = 15/22 (68%) Frame = +3 Query: 540 ETIQAKNALESYCFSMKSTMED 605 ET+ KNALE Y + ++ ++D Sbjct: 575 ETVDRKNALEEYIYDTRAKLDD 596 >SPAC31A2.09c |apm4||AP-2 adaptor complex subunit Apm4 |Schizosaccharomyces pombe|chr 1|||Manual Length = 446 Score = 25.8 bits (54), Expect = 7.4 Identities = 19/63 (30%), Positives = 30/63 (47%), Gaps = 7/63 (11%) Frame = -1 Query: 817 GNSLRAPLGNNLRK---LSGFVKCPFPNS----FRLGAHNSSFVGQAGWNPSHLDGGRAL 659 GN LR+ + ++ LSG +C F + F+L S NPS ++GG + Sbjct: 196 GNVLRSDVSGVVKMRAMLSGMPECQFGLNDKLDFKLKQSESKSKSNNSRNPSSVNGGFVI 255 Query: 658 VED 650 +ED Sbjct: 256 LED 258 >SPBC16A3.11 |eso1||sister chromatid cohesion protein Eso1|Schizosaccharomyces pombe|chr 2|||Manual Length = 872 Score = 25.8 bits (54), Expect = 7.4 Identities = 13/27 (48%), Positives = 18/27 (66%) Frame = +3 Query: 516 RNEDDKQKETIQAKNALESYCFSMKST 596 RN+ +KQ TI KNA++ Y S+K T Sbjct: 255 RNKPNKQ--TILTKNAIQDYLVSLKIT 279 >SPBC3H7.02 |||sulfate transporter |Schizosaccharomyces pombe|chr 2|||Manual Length = 877 Score = 25.4 bits (53), Expect = 9.7 Identities = 23/97 (23%), Positives = 41/97 (42%), Gaps = 5/97 (5%) Frame = +1 Query: 349 GVPQIEVTFDIDANGILNVSAIEKSINKEN---KITITNDKGRLSKEEIERMVN--EAGS 513 G+ +I FD D N I V I +N++ +T+ + + ++ AG Sbjct: 561 GILKIANKFDDDENSIDVVRDIYVPLNQKGMNPNLTVRDPPAGVLIFRLQESFTYPNAGH 620 Query: 514 TETRMTSKRRPSRPRMHWNLTASA*SLPWRMRSSREK 624 + +TSK + R + N+ A PW + R+K Sbjct: 621 VNSMLTSKAKTVTRRGNANIYKKASDRPWNDPAPRKK 657 >SPBC1734.01c ||SPBC337.17c|RNA-binding protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 682 Score = 25.4 bits (53), Expect = 9.7 Identities = 12/26 (46%), Positives = 15/26 (57%) Frame = +1 Query: 427 NKENKITITNDKGRLSKEEIERMVNE 504 NK+ K T D SKEE+E +V E Sbjct: 529 NKKGKHTQIEDPTAASKEELENLVRE 554 >SPAPB18E9.02c |ppk18||serine/threonine protein kinase Ppk18 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1316 Score = 25.4 bits (53), Expect = 9.7 Identities = 15/50 (30%), Positives = 23/50 (46%) Frame = -3 Query: 422 DFSIAETLRIPLASMSKVTSI*GTPRGAGGIPVSSNLPSKLLSLVIARST 273 + S L IP+AS K S T G G S + P L++ + R++ Sbjct: 479 NLSRRNNLAIPIASRRKSVSAVNTLYGVGSSYTSESFPFSKLTVPVERNS 528 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,337,268 Number of Sequences: 5004 Number of extensions: 67091 Number of successful extensions: 235 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 215 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 233 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 398435810 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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