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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS30585
         (814 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g01320.1 68414.m00048 tetratricopeptide repeat (TPR)-containi...    31   0.91 
At3g43300.1 68416.m04570 guanine nucleotide exchange family prot...    30   1.6  
At5g05850.1 68418.m00643 leucine-rich repeat family protein cont...    29   2.8  
At1g78160.1 68414.m09108 pumilio/Puf RNA-binding domain-containi...    29   2.8  
At4g19510.2 68417.m02870 disease resistance protein (TIR-NBS-LRR...    28   6.4  
At4g19510.1 68417.m02869 disease resistance protein (TIR-NBS-LRR...    28   6.4  
At2g05635.1 68415.m00600 hypothetical protein                          28   8.5  

>At1g01320.1 68414.m00048 tetratricopeptide repeat (TPR)-containing
           protein low similarity to SP|P46825 Kinesin light chain
           (KLC) {Loligo pealeii}; contains Pfam profile PF00515:
           TPR Domain
          Length = 1797

 Score = 31.1 bits (67), Expect = 0.91
 Identities = 24/77 (31%), Positives = 33/77 (42%), Gaps = 2/77 (2%)
 Frame = +2

Query: 569 CQHQIISIDKLKLRCVLLKDFASVMGSGGGFD*CAFYFLNLSSKRNYFGKGPFGLN-GTF 745
           C+    SI K ++ C  L D    +     FD      L   S+RN FG  P+G    T+
Sbjct: 228 CRKGFYSIGKQRIICHNLVDLLRQISRA--FDNAYSDLLKAFSERNKFGNLPYGFRANTW 285

Query: 746 VLPP-GNSIPRNSKPLP 793
           ++PP     P    PLP
Sbjct: 286 LIPPTAAQSPAAFPPLP 302


>At3g43300.1 68416.m04570 guanine nucleotide exchange family protein
            similar to SP|Q9Y6D5 Brefeldin A-inhibited guanine
            nucleotide-exchange protein 2 {Homo sapiens}; contains
            Pfam profile PF01369: Sec7 domain
          Length = 1756

 Score = 30.3 bits (65), Expect = 1.6
 Identities = 20/76 (26%), Positives = 36/76 (47%), Gaps = 2/76 (2%)
 Frame = +1

Query: 238  RTIRIWSYCT--VDEFFARCFNEPEQNMLFKKFLDGVCTEKPTVQCETFKTKSEGELVEI 411
            ++IR  SY T  ++   A  F+ P++N++    ++   ++ P V       K  G++   
Sbjct: 1395 KSIRDASYTTQPLELLNALSFDNPKKNLVLAGDIEADASDSPRVDRNPDDIKDNGKVSAQ 1454

Query: 412  KNPRFPHKNTQEESGI 459
             +PR     T  ESGI
Sbjct: 1455 ASPRIGTHGTSLESGI 1470


>At5g05850.1 68418.m00643 leucine-rich repeat family protein
           contains Pfam PF00560: Leucine Rich Repeat domains;
           similar to  (SP:Q9UQ13) Leucine-rich repeat protein
           SHOC-2 (Ras-binding protein Sur-8) (SP:Q9UQ13) {Homo
           sapiens}
          Length = 506

 Score = 29.5 bits (63), Expect = 2.8
 Identities = 24/94 (25%), Positives = 42/94 (44%), Gaps = 5/94 (5%)
 Frame = +2

Query: 38  AAPD--AAIHELTFVD---NEIETIHPNSLGFIGQAEAHSANSIKYKNNYYGERCHCNIS 202
           A PD  A +H L  +D   N +ET+ P+S+G + + +  + +  K         CHC   
Sbjct: 241 AIPDSIAGLHNLLELDVSTNFLETL-PDSIGLLSKLKILNVSCNKL-TTLPDSICHCGSL 298

Query: 203 QVLKRSLNVVSIEPYEFGVTVLSMNSSRVALTNL 304
            VL  S N ++  P   G  ++ +    + L  +
Sbjct: 299 VVLDASYNNLTYLPTNIGFELVKLEKLLIHLNKI 332


>At1g78160.1 68414.m09108 pumilio/Puf RNA-binding domain-containing
           protein contains Pfam profile: PF00806 Pumilio-family
           RNA binding domains (aka PUM-HD, Pumilio homology
           domain) (8 copies at C-terminal half of protein)
          Length = 650

 Score = 29.5 bits (63), Expect = 2.8
 Identities = 15/38 (39%), Positives = 21/38 (55%)
 Frame = +1

Query: 298 EPEQNMLFKKFLDGVCTEKPTVQCETFKTKSEGELVEI 411
           +P  N L +K LD VCTE+   Q     T+  G+L+ I
Sbjct: 380 DPFGNYLMQKLLD-VCTEEQRTQIVLVATEEPGQLIRI 416


>At4g19510.2 68417.m02870 disease resistance protein (TIR-NBS-LRR
           class), putative domain signature TIR-NBS-LRR exists,
           suggestive of a disease resistance protein.
          Length = 1049

 Score = 28.3 bits (60), Expect = 6.4
 Identities = 15/51 (29%), Positives = 27/51 (52%)
 Frame = -1

Query: 742 GSIQSEWSFPEVIPFTG*IEKIERALIKAPATSHYRGKILEQNAPQLEFVD 590
           GS ++ +  P  I F G  +KIE   +     S+ RG +  +N+ + +F+D
Sbjct: 121 GSFEAVFQSPTKI-FNGDEQKIEELKVALKTASNIRGFVYPENSSEPDFLD 170


>At4g19510.1 68417.m02869 disease resistance protein (TIR-NBS-LRR
           class), putative domain signature TIR-NBS-LRR exists,
           suggestive of a disease resistance protein.
          Length = 1049

 Score = 28.3 bits (60), Expect = 6.4
 Identities = 15/51 (29%), Positives = 27/51 (52%)
 Frame = -1

Query: 742 GSIQSEWSFPEVIPFTG*IEKIERALIKAPATSHYRGKILEQNAPQLEFVD 590
           GS ++ +  P  I F G  +KIE   +     S+ RG +  +N+ + +F+D
Sbjct: 121 GSFEAVFQSPTKI-FNGDEQKIEELKVALKTASNIRGFVYPENSSEPDFLD 170


>At2g05635.1 68415.m00600 hypothetical protein
          Length = 146

 Score = 27.9 bits (59), Expect = 8.5
 Identities = 19/63 (30%), Positives = 29/63 (46%), Gaps = 4/63 (6%)
 Frame = +2

Query: 38  AAPDAAIHELT----FVDNEIETIHPNSLGFIGQAEAHSANSIKYKNNYYGERCHCNISQ 205
           AAP+AA   L     F+    +   P S+  + +AE  +    K    YY  R H  I+Q
Sbjct: 51  AAPEAATDPLNHGGGFIPETQQRDTPASIN-VEKAETATKKRTKIPTIYYASRTHSQITQ 109

Query: 206 VLK 214
           V++
Sbjct: 110 VIR 112


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,060,890
Number of Sequences: 28952
Number of extensions: 337262
Number of successful extensions: 873
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 842
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 873
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1853336000
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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