Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30584
(806 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g28220.1 68415.m03426 aspartyl protease family protein contai... 32 0.52
At5g46070.1 68418.m05665 guanylate-binding family protein contai... 30 1.6
At1g02840.3 68414.m00246 pre-mRNA splicing factor SF2 (SF2) / SR... 30 1.6
At1g02840.1 68414.m00245 pre-mRNA splicing factor SF2 (SF2) / SR... 30 1.6
At5g01840.1 68418.m00103 ovate family protein 59% similar to ova... 29 2.7
At3g28430.1 68416.m03553 expressed protein non-consensus GC dono... 29 2.7
At4g29710.1 68417.m04231 phosphodiesterase/nucleotide pyrophosph... 29 4.8
At4g23580.1 68417.m03397 kelch repeat-containing F-box family pr... 28 6.3
At3g63150.1 68416.m07092 GTP-binding protein-related low similar... 28 6.3
At3g28670.1 68416.m03578 expressed protein 28 6.3
At1g27960.1 68414.m03425 expressed protein contains Pfam profile... 28 6.3
At1g12200.1 68414.m01412 flavin-containing monooxygenase family ... 28 6.3
At4g28670.1 68417.m04097 protein kinase family protein contains ... 28 8.4
At2g01950.1 68415.m00130 leucine-rich repeat transmembrane prote... 28 8.4
>At2g28220.1 68415.m03426 aspartyl protease family protein contains
Pfam domain, PF00026: eukaryotic aspartyl protease
Length = 756
Score = 31.9 bits (69), Expect = 0.52
Identities = 18/66 (27%), Positives = 35/66 (53%)
Frame = +1
Query: 244 VVDTAPTIRSPWTRRPSSLCS*KKISETEPWSLYQLPEPSGKESPSYWTSS*DICTISTE 423
V+D+ T+ T P S C+ + + + + ++P+PSG + Y++ + DI + T
Sbjct: 291 VIDSGSTV----TYFPVSYCNLVRKAVEQVVTAVRVPDPSGNDMLCYFSETIDIFPVITM 346
Query: 424 HVAAGA 441
H + GA
Sbjct: 347 HFSGGA 352
>At5g46070.1 68418.m05665 guanylate-binding family protein contains
Pfam domains PF02263: Guanylate-binding protein,
N-terminal domain and PF02841: Guanylate-binding
protein, C-terminal domain
Length = 1060
Score = 30.3 bits (65), Expect = 1.6
Identities = 20/44 (45%), Positives = 28/44 (63%), Gaps = 1/44 (2%)
Frame = +3
Query: 270 FTLDEEALKSL-LLKEDIRDRAVVVISVAGAFRKGKSFLLDFFL 398
F +D EA+ +L L+KE I V+SV G R+GKSF+L+ L
Sbjct: 53 FRMDPEAVATLQLVKEPIG-----VVSVCGRARQGKSFILNQLL 91
>At1g02840.3 68414.m00246 pre-mRNA splicing factor SF2 (SF2) / SR1
protein identical to SP|O22315 Pre-mRNA splicing factor
SF2 (SR1 protein) {Arabidopsis thaliana}
Length = 303
Score = 30.3 bits (65), Expect = 1.6
Identities = 23/66 (34%), Positives = 32/66 (48%), Gaps = 1/66 (1%)
Frame = -2
Query: 316 SSFRSRDLRASSSRVNVWSGPCRRPGHRAAHAVPAPASLSLSRTSSRIPR-SFSRSLMPN 140
S RSR +A SSR + R PG R+ P+P S SR+ S +P S P+
Sbjct: 229 SRSRSRSPKAKSSRRSPAKSTSRSPGPRSKSRSPSPRR-SRSRSRSPLPSVQKEGSKSPS 287
Query: 139 EPVKAR 122
+P A+
Sbjct: 288 KPSPAK 293
>At1g02840.1 68414.m00245 pre-mRNA splicing factor SF2 (SF2) / SR1
protein identical to SP|O22315 Pre-mRNA splicing factor
SF2 (SR1 protein) {Arabidopsis thaliana}
Length = 303
Score = 30.3 bits (65), Expect = 1.6
Identities = 23/66 (34%), Positives = 32/66 (48%), Gaps = 1/66 (1%)
Frame = -2
Query: 316 SSFRSRDLRASSSRVNVWSGPCRRPGHRAAHAVPAPASLSLSRTSSRIPR-SFSRSLMPN 140
S RSR +A SSR + R PG R+ P+P S SR+ S +P S P+
Sbjct: 229 SRSRSRSPKAKSSRRSPAKSTSRSPGPRSKSRSPSPRR-SRSRSRSPLPSVQKEGSKSPS 287
Query: 139 EPVKAR 122
+P A+
Sbjct: 288 KPSPAK 293
>At5g01840.1 68418.m00103 ovate family protein 59% similar to ovate
protein (GI:23429649) [Lycopersicon esculentum];
contains TIGRFAM TIGR01568 : uncharacterized
plant-specific domain TIGR01568
Length = 270
Score = 29.5 bits (63), Expect = 2.7
Identities = 20/65 (30%), Positives = 32/65 (49%), Gaps = 1/65 (1%)
Frame = -2
Query: 310 FRSRDLRASSSRVNVWSGPCRRPGHRAAHAVPAPASLS-LSRTSSRIPRSFSRSLMPNEP 134
++ RD+ + S + N+ S P + HAVP P S + LS R P S++ + P
Sbjct: 19 YKLRDM-SKSKKKNLQSQPNSTTSKKKHHAVPTPTSTTPLSPRPPRRPSHSSKAPPSHPP 77
Query: 133 VKARG 119
K+ G
Sbjct: 78 RKSSG 82
>At3g28430.1 68416.m03553 expressed protein non-consensus GC donor
splice site at exon 16
Length = 837
Score = 29.5 bits (63), Expect = 2.7
Identities = 11/26 (42%), Positives = 17/26 (65%)
Frame = +3
Query: 354 GAFRKGKSFLLDFFLRYMHYKYGTCC 431
G+ R G S LD++LR + ++G CC
Sbjct: 478 GSMRDGLSSELDWYLRRLEEQFGVCC 503
>At4g29710.1 68417.m04231 phosphodiesterase/nucleotide
pyrophosphatase-related weak similarity to SP|Q13822
Ectonucleotide pyrophosphatase/phosphodiesterase 2 {Homo
sapiens}
Length = 133
Score = 28.7 bits (61), Expect = 4.8
Identities = 16/36 (44%), Positives = 24/36 (66%)
Frame = +2
Query: 614 HGARLRDRVRSVPTMLSSVQIYNLSQNIPGGRPSSN 721
HG+R R R + VP+ +VQIY++ +I G RP+ N
Sbjct: 79 HGSRFR-RGKKVPSF-ENVQIYSVVADILGLRPAPN 112
>At4g23580.1 68417.m03397 kelch repeat-containing F-box family
protein contains F-box domain Pfam:PF00646 and Kelch
motif Pfam:PF01344
Length = 383
Score = 28.3 bits (60), Expect = 6.3
Identities = 11/34 (32%), Positives = 18/34 (52%)
Frame = -2
Query: 709 SSSWNVLREVVYLNGRQHRWNRANTVAQSRTVDS 608
S ++N L V+ +N R H W+ A ++ R S
Sbjct: 140 SKTYNALSSVMIMNSRSHTWHEAPSMRVGRVFPS 173
>At3g63150.1 68416.m07092 GTP-binding protein-related low similarity
to SP|Q38912 RAC-like GTP binding protein ARAC3
(GTP-binding protein ROP6) {Arabidopsis thaliana};
contains Pfam profile PF00036: EF hand (domain)
Length = 643
Score = 28.3 bits (60), Expect = 6.3
Identities = 19/52 (36%), Positives = 27/52 (51%)
Frame = +3
Query: 243 GRRHGPDHTFTLDEEALKSLLLKEDIRDRAVVVISVAGAFRKGKSFLLDFFL 398
G H P TF++ + +S+ K+ +R V V G + GKS LLD FL
Sbjct: 397 GYGHDPASTFSVTRK--RSVDRKKQRTERNVFQCFVFGPKKSGKSALLDSFL 446
>At3g28670.1 68416.m03578 expressed protein
Length = 491
Score = 28.3 bits (60), Expect = 6.3
Identities = 20/66 (30%), Positives = 30/66 (45%), Gaps = 1/66 (1%)
Frame = -2
Query: 235 RAAH-AVPAPASLSLSRTSSRIPRSFSRSLMPNEPVKARG*PCAPFLAAQDHDPRSNYKK 59
R +H A+PA L + + SS + R F L+P P G P L +D + + K
Sbjct: 18 RLSHSAIPAEVGLVIGKLSSVLDRGFVFDLIPT-PSNDDGEPACAVLETKDDKRKPSKSK 76
Query: 58 NPISMS 41
+ S S
Sbjct: 77 SQSSES 82
>At1g27960.1 68414.m03425 expressed protein contains Pfam profile
PF04146: YT521-B-like family
Length = 539
Score = 28.3 bits (60), Expect = 6.3
Identities = 9/26 (34%), Positives = 15/26 (57%)
Frame = -2
Query: 802 PAWKREFRAPPFLEAASRTRNSVNNC 725
P W+ ++ PP EA S + N ++C
Sbjct: 259 PTWENDYNLPPLDEARSESYNDFSHC 284
>At1g12200.1 68414.m01412 flavin-containing monooxygenase family
protein / FMO family protein low similarity to FMO2 from
Homo sapiens [SP|Q99518]; contains Pfam profile: PF00743
Flavin-binding monooxygenase-like
Length = 465
Score = 28.3 bits (60), Expect = 6.3
Identities = 25/84 (29%), Positives = 36/84 (42%)
Frame = -2
Query: 304 SRDLRASSSRVNVWSGPCRRPGHRAAHAVPAPASLSLSRTSSRIPRSFSRSLMPNEPVKA 125
+R+LR V V + G A + P LSL T + S RSL N P +
Sbjct: 27 ARELRREGHSVVVLERGSQIGGVWAYTSQVEPDPLSLDPTRPVVHSSLYRSLRTNIPREC 86
Query: 124 RG*PCAPFLAAQDHDPRSNYKKNP 53
G PF A + HD + +++P
Sbjct: 87 MGFTDFPF-ATRPHDGSRDPRRHP 109
>At4g28670.1 68417.m04097 protein kinase family protein contains
Pfam domain, PF00069: Protein kinase domain
Length = 625
Score = 27.9 bits (59), Expect = 8.4
Identities = 19/58 (32%), Positives = 31/58 (53%)
Frame = +2
Query: 512 GILMWSEIFKATLDDGEKVAIILFRHARRL*Q*IHGARLRDRVRSVPTMLSSVQIYNL 685
G+ + E+FK TL DG ++AI +RL + G + RD + + ++S Q NL
Sbjct: 338 GVGGYGEVFKGTLSDGREIAI------KRL--HVSGKKPRDEIHNEIDVISRCQHKNL 387
>At2g01950.1 68415.m00130 leucine-rich repeat transmembrane protein
kinase, putative similar to brassinosteroid insensitive
protein
Length = 1143
Score = 27.9 bits (59), Expect = 8.4
Identities = 14/24 (58%), Positives = 16/24 (66%)
Frame = +2
Query: 503 GHTGILMWSEIFKATLDDGEKVAI 574
GH G + E+FKATL DG VAI
Sbjct: 845 GHGG---FGEVFKATLKDGSSVAI 865
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,319,171
Number of Sequences: 28952
Number of extensions: 401995
Number of successful extensions: 1371
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 1315
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1371
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1833827200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -