Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30581
(762 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g34670.1 68417.m04922 40S ribosomal protein S3A (RPS3aB) 143 1e-34
At3g04840.1 68416.m00525 40S ribosomal protein S3A (RPS3aA) simi... 138 5e-33
At5g56890.1 68418.m07099 protein kinase family protein contains ... 31 0.63
At3g52640.1 68416.m05799 nicastrin-related contains weak similar... 30 1.5
At5g45490.1 68418.m05588 disease resistance protein-related cont... 29 3.4
At4g22860.1 68417.m03302 expressed protein 29 4.5
>At4g34670.1 68417.m04922 40S ribosomal protein S3A (RPS3aB)
Length = 262
Score = 143 bits (347), Expect = 1e-34
Identities = 68/135 (50%), Positives = 95/135 (70%), Gaps = 3/135 (2%)
Frame = +3
Query: 255 DLTTDKLRWMVKKWQTLIEANIDVKTTDGYVLRVFCIGFTNKDSLSQRKTCYAQHTQVRA 434
D TTDKLR +VKKWQTLIEA++DVKTTDGY LR+FCI FT + + ++TCYAQ +Q+R
Sbjct: 104 DFTTDKLRSLVKKWQTLIEAHVDVKTTDGYTLRMFCIAFTKRRANQVKRTCYAQSSQIRQ 163
Query: 435 IRKKMCEIITRDVTNSELREVVNKLFLTPLPRTSRRPAMGIYPLRDVCIRK*KC*RGPVF 614
IR+KM EI+ ++ ++ +L+E+V K + R + GIYPL++V IRK K + P F
Sbjct: 164 IRRKMSEIMVKEASSCDLKELVAKFIPEAIGREIEKATQGIYPLQNVFIRKVKILKAPKF 223
Query: 615 EISKLM---GTFTGE 650
++ KLM G +T E
Sbjct: 224 DLGKLMEVHGDYTAE 238
Score = 123 bits (297), Expect = 1e-28
Identities = 62/85 (72%), Positives = 68/85 (80%)
Frame = +1
Query: 1 VDPFTRKDWYDVKAPSMFSKRQVGTTLVNRTQGTKIASEGLKGRVFEVSLADLQADTDAE 180
VDPF++KDWYDVKAP F+ R VG TLV+RTQGTKIASEGLK RVFEVSLADLQ D D
Sbjct: 21 VDPFSKKDWYDVKAPGSFTNRNVGKTLVSRTQGTKIASEGLKHRVFEVSLADLQNDED-- 78
Query: 181 RSFRKFRLIAEYVQGRNVLCNFHGM 255
++RK RL AE VQGRNVL F GM
Sbjct: 79 NAYRKIRLRAEDVQGRNVLTQFWGM 103
>At3g04840.1 68416.m00525 40S ribosomal protein S3A (RPS3aA) similar
to 40S ribosomal protein S3A (S phase specific protein
GBIS289) GB:P49396 [Brassica rapa]
Length = 262
Score = 138 bits (333), Expect = 5e-33
Identities = 63/126 (50%), Positives = 90/126 (71%)
Frame = +3
Query: 255 DLTTDKLRWMVKKWQTLIEANIDVKTTDGYVLRVFCIGFTNKDSLSQRKTCYAQHTQVRA 434
D TTDKLR +VKKWQTLIEA++DVKTTD Y LR+FCI FT + + ++TCYAQ +Q+R
Sbjct: 104 DFTTDKLRSLVKKWQTLIEAHVDVKTTDSYTLRLFCIAFTKRRANQVKRTCYAQSSQIRQ 163
Query: 435 IRKKMCEIITRDVTNSELREVVNKLFLTPLPRTSRRPAMGIYPLRDVCIRK*KC*RGPVF 614
IR+KM +I+ R+ ++ +L+++V K + R + GIYPL++V IRK K + P F
Sbjct: 164 IRRKMRDIMVREASSCDLKDLVAKFIPEAIGREIEKATQGIYPLQNVFIRKVKILKAPKF 223
Query: 615 EISKLM 632
++ KLM
Sbjct: 224 DLGKLM 229
Score = 130 bits (314), Expect = 1e-30
Identities = 64/85 (75%), Positives = 71/85 (83%)
Frame = +1
Query: 1 VDPFTRKDWYDVKAPSMFSKRQVGTTLVNRTQGTKIASEGLKGRVFEVSLADLQADTDAE 180
VDPF++KDWYDVKAPS+F+ R VG TLV+RTQGTKIASEGLK RVFEVSLADLQ D D
Sbjct: 21 VDPFSKKDWYDVKAPSIFTHRNVGKTLVSRTQGTKIASEGLKHRVFEVSLADLQGDED-- 78
Query: 181 RSFRKFRLIAEYVQGRNVLCNFHGM 255
++RK RL AE VQGRNVLC F GM
Sbjct: 79 NAYRKIRLRAEDVQGRNVLCQFWGM 103
>At5g56890.1 68418.m07099 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 1113
Score = 31.5 bits (68), Expect = 0.63
Identities = 12/40 (30%), Positives = 23/40 (57%)
Frame = -3
Query: 721 REGFVSPPAVSDLSPAFPLASNRPSPVKVPINFEISKTGP 602
R+G ++PP S +P+ P+ + SP P++F + + P
Sbjct: 377 RKGSMTPPPQSHHAPSPPIPDSLISPAHAPVSFSMKRISP 416
>At3g52640.1 68416.m05799 nicastrin-related contains weak similarity
to Nicastrin precursor (Swiss-Prot:Q92542) [Homo
sapiens]
Length = 676
Score = 30.3 bits (65), Expect = 1.5
Identities = 19/58 (32%), Positives = 32/58 (55%), Gaps = 3/58 (5%)
Frame = -2
Query: 461 NDFTH-FLSDCSDLSVLGVARLA--LAQGVLIGETNAEDT*NVSIGCLHINVGFDESL 297
N F H L D S+++ V A +A+ + I ++ +DT N ++G +H+N F E L
Sbjct: 430 NKFYHSHLDDLSNINSSSVVAAASVVARTLYILASDNKDTSNSALGSIHVNASFVEEL 487
>At5g45490.1 68418.m05588 disease resistance protein-related
contains Pfam domain, PF00931: NB-ARC domain, a novel
signalling motif found in plant resistance gene products
Length = 354
Score = 29.1 bits (62), Expect = 3.4
Identities = 22/80 (27%), Positives = 33/80 (41%), Gaps = 4/80 (5%)
Frame = +1
Query: 13 TRKDWYDVK--APSMFSKRQVGTTLVNRTQG-TKIASEGLKGRVFEV-SLADLQADTDAE 180
T KDW DV A K++ R + +K+ + G E+ SL D
Sbjct: 22 TCKDWLDVNGLAKGNLEKKRDDNEEEERLKTESKLPGHDIHGFDNEIKSLQHFLLDQKVR 81
Query: 181 RSFRKFRLIAEYVQGRNVLC 240
R F+ ++ EY G+ LC
Sbjct: 82 REFKSLVIVGEYGVGKTALC 101
>At4g22860.1 68417.m03302 expressed protein
Length = 509
Score = 28.7 bits (61), Expect = 4.5
Identities = 18/70 (25%), Positives = 31/70 (44%)
Frame = +1
Query: 238 CNFHGMTSQPISSGGWLKNGRLSSKPTLM*RQPMDTFYVSSALVSPIRTP*ANARRATPS 417
CN + +QPI G + N + + KP + + SS L+ P + A A+
Sbjct: 128 CNLKKVPNQPIWKGPTVSNHKHTDKPKFRAKSSIRPTPRSSTLMRPTASQLAKQNNASKF 187
Query: 418 TLRSEQSERK 447
++ +QS K
Sbjct: 188 HMQVDQSHDK 197
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,018,671
Number of Sequences: 28952
Number of extensions: 404378
Number of successful extensions: 1272
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 1204
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1268
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1702303248
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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