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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS30574
         (641 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g28770.1 68416.m03591 expressed protein                             32   0.28 
At1g28400.1 68414.m03492 expressed protein similar to E6 (GI:100...    31   0.49 
At5g61080.1 68418.m07664 expressed protein predicted proteins, A...    30   1.5  
At5g18860.1 68418.m02241 inosine-uridine preferring nucleoside h...    29   2.6  
At2g03630.1 68415.m00323 hypothetical protein                          29   2.6  
At3g03230.1 68416.m00319 esterase/lipase/thioesterase family pro...    28   4.6  
At2g28640.1 68415.m03482 exocyst subunit EXO70 family protein co...    28   4.6  
At4g10840.2 68417.m01764 kinesin light chain-related low similar...    28   6.1  
At4g10840.1 68417.m01765 kinesin light chain-related low similar...    28   6.1  
At2g03140.1 68415.m00267 CAAX amino terminal protease family pro...    28   6.1  
At2g34170.1 68415.m04182 expressed protein contains Pfam profile...    27   8.0  
At2g07360.1 68415.m00843 SH3 domain-containing protein contains ...    27   8.0  
At2g04620.1 68415.m00470 cation efflux family protein potential ...    27   8.0  
At1g67650.1 68414.m07720 expressed protein                             27   8.0  
At1g33980.1 68414.m04213 Smg-4/UPF3 family protein contains Pfam...    27   8.0  

>At3g28770.1 68416.m03591 expressed protein 
          Length = 2081

 Score = 32.3 bits (70), Expect = 0.28
 Identities = 27/110 (24%), Positives = 44/110 (40%)
 Frame = +1

Query: 304  HQDNKTFSTQSETHTKQSHTEHSSIKHVHSGTHKYITTSEDINNRTTSEDINDRTISQKR 483
            H+DNK+   + +   K+ H E  S K       K +   ED N+    ED N++  SQ  
Sbjct: 1087 HEDNKSMKKEEDKKEKKKHEESKSRKKEED--KKDMEKLEDQNSNKKKEDKNEKKKSQHV 1144

Query: 484  PEFNIIFGFIERTRSPSVNDRNVVRSSTDRKIINKTDIKNVTNVSTSVKK 633
                      E+  +   ++   + SS  +K  N+ D K   +     KK
Sbjct: 1145 KLVKKESDKKEKKENEEKSETKEIESSKSQK--NEVDKKEKKSSKDQQKK 1192


>At1g28400.1 68414.m03492 expressed protein similar to E6
           (GI:1000090) [Gossypium barbadense]
          Length = 335

 Score = 31.5 bits (68), Expect = 0.49
 Identities = 21/65 (32%), Positives = 32/65 (49%), Gaps = 3/65 (4%)
 Frame = +1

Query: 274 QFNNRKTTAIHQDNKTFSTQSETHTKQS---HTEHSSIKHVHSGTHKYITTSEDINNRTT 444
           +FNN        ++KTFST S + T++S   + E+   K  + GT  Y   +E+ NN   
Sbjct: 100 EFNNNNKNDEKVNSKTFSTPSLSETEESFNNYEENYPKKTENYGTKGY--NNEEFNNNNN 157

Query: 445 SEDIN 459
             D N
Sbjct: 158 KYDAN 162


>At5g61080.1 68418.m07664 expressed protein predicted proteins,
           Arabidopsis thaliana; expression supported by MPSS
          Length = 348

 Score = 29.9 bits (64), Expect = 1.5
 Identities = 16/57 (28%), Positives = 30/57 (52%)
 Frame = +2

Query: 35  TPGETPSQIRPKDNLRPEGDFDRPDTQKWVPSEKSGTFRPKDNITRNGEIIREDSYK 205
           TP  +P +++ + ++ P   F++  T   V     GT  P  ++TR+  I+R+ S K
Sbjct: 168 TPSRSPEKVKGEASVPP--GFEKIWTPPLVKLNIQGTSNPLSDLTRSAGIVRDQSGK 222


>At5g18860.1 68418.m02241 inosine-uridine preferring nucleoside
           hydrolase family protein contains Pfam profile PF01156:
           Inosine-uridine preferring nucleoside hydrolase
          Length = 890

 Score = 29.1 bits (62), Expect = 2.6
 Identities = 30/85 (35%), Positives = 39/85 (45%), Gaps = 9/85 (10%)
 Frame = +2

Query: 17  RSPTRYTPGETPSQIRPKDNLRPEGDFDRP-DTQKWVPSEKSGTFRPKDNITRNG----- 178
           RSP RYT   + +   P+D  RPE    +P   + W    KSG    K  +  NG     
Sbjct: 620 RSPRRYTAENSVTHGAPRDTDRPE--LRQPLAIEVWQNLTKSGNGVSKITVLTNGPLTNL 677

Query: 179 -EIIREDSYKSSEGVKK--IVGSKI 244
            +II  D  KSS  +K+  IVG  I
Sbjct: 678 AKIISSDK-KSSSLIKEVYIVGGHI 701


>At2g03630.1 68415.m00323 hypothetical protein
          Length = 252

 Score = 29.1 bits (62), Expect = 2.6
 Identities = 16/56 (28%), Positives = 28/56 (50%)
 Frame = +1

Query: 307 QDNKTFSTQSETHTKQSHTEHSSIKHVHSGTHKYITTSEDINNRTTSEDINDRTIS 474
           Q+ +T    SE+H++ S +  SS     S +  Y++  +D+      E +ND  IS
Sbjct: 40  QETETVIINSESHSRLSSSSSSS-----SSSSSYLSPPKDLPEEVLKESLNDPEIS 90


>At3g03230.1 68416.m00319 esterase/lipase/thioesterase family
           protein contains Interpro entry IPR000379
          Length = 333

 Score = 28.3 bits (60), Expect = 4.6
 Identities = 13/38 (34%), Positives = 19/38 (50%)
 Frame = +1

Query: 316 KTFSTQSETHTKQSHTEHSSIKHVHSGTHKYITTSEDI 429
           K   T+ +    + HT+HS IK        Y+TT +DI
Sbjct: 215 KDTMTERDLELAEKHTKHSYIKESALRQGGYVTTQQDI 252


>At2g28640.1 68415.m03482 exocyst subunit EXO70 family protein
           contains HEAT repeat and Pfam domain PF03081:exocyst
           subunit EXO70
          Length = 605

 Score = 28.3 bits (60), Expect = 4.6
 Identities = 17/61 (27%), Positives = 29/61 (47%)
 Frame = -2

Query: 190 SNDFSISSYIVFGPEGTRLFRWHPFLSIRTIKIAFRSQVIFRSNLRRCFTWSVSCRRTRS 11
           S  ++I   ++  PE  +L R H  ++I   ++      I +SN R     SVS R + S
Sbjct: 76  SLQYAIQGVVLVNPESPKLTRAHNLVTIAMKQLEKEFYRILKSNRRNLDPESVSVRSSPS 135

Query: 10  Y 8
           +
Sbjct: 136 F 136


>At4g10840.2 68417.m01764 kinesin light chain-related low similarity
           to kinesin light chain [Plectonema boryanum] GI:2645229;
           contains Pfam profile PF00515 TPR Domain
          Length = 531

 Score = 27.9 bits (59), Expect = 6.1
 Identities = 13/30 (43%), Positives = 17/30 (56%)
 Frame = +2

Query: 17  RSPTRYTPGETPSQIRPKDNLRPEGDFDRP 106
           R+P + TP  TPS+ +P  N R  G  D P
Sbjct: 34  RTPMKKTPSSTPSRSKPSPN-RSTGKKDSP 62


>At4g10840.1 68417.m01765 kinesin light chain-related low similarity
           to kinesin light chain [Plectonema boryanum] GI:2645229;
           contains Pfam profile PF00515 TPR Domain
          Length = 609

 Score = 27.9 bits (59), Expect = 6.1
 Identities = 13/30 (43%), Positives = 17/30 (56%)
 Frame = +2

Query: 17  RSPTRYTPGETPSQIRPKDNLRPEGDFDRP 106
           R+P + TP  TPS+ +P  N R  G  D P
Sbjct: 34  RTPMKKTPSSTPSRSKPSPN-RSTGKKDSP 62


>At2g03140.1 68415.m00267 CAAX amino terminal protease family protein
            very low similarity to SP|Q40863 Late embryogenesis
            abundant protein EMB8 from Picea glauca; contains Pfam
            profile PF02517 CAAX amino terminal protease family
            protein
          Length = 1805

 Score = 27.9 bits (59), Expect = 6.1
 Identities = 19/101 (18%), Positives = 42/101 (41%), Gaps = 3/101 (2%)
 Frame = +1

Query: 301  IHQDNKTFSTQSETHTKQSHTEHSSIKHVHSGTHKYITTSEDINNRTTSED---INDRTI 471
            + Q +   + Q +  T ++  + + I +  S        + D  N +T+      +D T 
Sbjct: 791  VEQGSGVATHQGQVETSKNDEKGAPIANEKSSVADGFEKASDAKNDSTNPQPVGADDITS 850

Query: 472  SQKRPEFNIIFGFIERTRSPSVNDRNVVRSSTDRKIINKTD 594
               + +  ++    +R    S +D N  +S+TD+  +  TD
Sbjct: 851  DGDKVDQGVVLAQQQRKDETSKSDENAKQSATDQNKVTSTD 891


>At2g34170.1 68415.m04182 expressed protein contains Pfam profile:
           PF05097 protein of unknown function (DUF688)
          Length = 523

 Score = 27.5 bits (58), Expect = 8.0
 Identities = 13/46 (28%), Positives = 21/46 (45%)
 Frame = +2

Query: 20  SPTRYTPGETPSQIRPKDNLRPEGDFDRPDTQKWVPSEKSGTFRPK 157
           +PTRY    TPS  +  D+   E D D  +  +++     G   P+
Sbjct: 161 TPTRYESSFTPSYYQDIDDEESEEDSDDDEVSEYLSKRGCGMMSPQ 206


>At2g07360.1 68415.m00843 SH3 domain-containing protein contains
           Pfam profile PF00018: SH3 domain
          Length = 1196

 Score = 27.5 bits (58), Expect = 8.0
 Identities = 17/59 (28%), Positives = 25/59 (42%)
 Frame = +1

Query: 319 TFSTQSETHTKQSHTEHSSIKHVHSGTHKYITTSEDINNRTTSEDINDRTISQKRPEFN 495
           T S+ S T ++ +    SS  H+H       T  E  + R T   I + TIS  +   N
Sbjct: 25  TSSSASSTASQPTSASASSSSHLHHPMSTKTTLGEKKSKRATLMQIQNDTISVAKAALN 83


>At2g04620.1 68415.m00470 cation efflux family protein potential
           member of the cation diffusion facilitator (CDF) family,
           or cation efflux (CE) family, see PMID:11500563
          Length = 798

 Score = 27.5 bits (58), Expect = 8.0
 Identities = 11/40 (27%), Positives = 20/40 (50%)
 Frame = +1

Query: 334 SETHTKQSHTEHSSIKHVHSGTHKYITTSEDINNRTTSED 453
           S++H  + H EH    H HS  H+    + D  +++ S +
Sbjct: 586 SDSHKHEEHHEHDHHHHSHSHKHEECNHNHDHEHQSHSHN 625


>At1g67650.1 68414.m07720 expressed protein
          Length = 651

 Score = 27.5 bits (58), Expect = 8.0
 Identities = 9/27 (33%), Positives = 16/27 (59%)
 Frame = +2

Query: 104 PDTQKWVPSEKSGTFRPKDNITRNGEI 184
           PD ++W+P  +  ++RPK    R  +I
Sbjct: 579 PDPERWLPRRERSSYRPKRKDKRAAQI 605


>At1g33980.1 68414.m04213 Smg-4/UPF3 family protein contains Pfam
           PF03467: Smg-4/UPF3 family; similar to hUPF3B
           (GI:12232324) [Homo sapiens]
          Length = 482

 Score = 27.5 bits (58), Expect = 8.0
 Identities = 16/47 (34%), Positives = 20/47 (42%), Gaps = 1/47 (2%)
 Frame = +2

Query: 98  DRPDTQKWVPSEKSGTFRPKDNITRNGEI-IREDSYKSSEGVKKIVG 235
           DRPD   W P       +P  +   NGE+  R  S +S E V    G
Sbjct: 380 DRPDRVMWAPRRDGSEDQPLSSAGNNGEVKDRMFSQRSGEVVNSSGG 426


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,802,860
Number of Sequences: 28952
Number of extensions: 219991
Number of successful extensions: 680
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 653
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 679
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1324661040
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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