BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30574 (641 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g28770.1 68416.m03591 expressed protein 32 0.28 At1g28400.1 68414.m03492 expressed protein similar to E6 (GI:100... 31 0.49 At5g61080.1 68418.m07664 expressed protein predicted proteins, A... 30 1.5 At5g18860.1 68418.m02241 inosine-uridine preferring nucleoside h... 29 2.6 At2g03630.1 68415.m00323 hypothetical protein 29 2.6 At3g03230.1 68416.m00319 esterase/lipase/thioesterase family pro... 28 4.6 At2g28640.1 68415.m03482 exocyst subunit EXO70 family protein co... 28 4.6 At4g10840.2 68417.m01764 kinesin light chain-related low similar... 28 6.1 At4g10840.1 68417.m01765 kinesin light chain-related low similar... 28 6.1 At2g03140.1 68415.m00267 CAAX amino terminal protease family pro... 28 6.1 At2g34170.1 68415.m04182 expressed protein contains Pfam profile... 27 8.0 At2g07360.1 68415.m00843 SH3 domain-containing protein contains ... 27 8.0 At2g04620.1 68415.m00470 cation efflux family protein potential ... 27 8.0 At1g67650.1 68414.m07720 expressed protein 27 8.0 At1g33980.1 68414.m04213 Smg-4/UPF3 family protein contains Pfam... 27 8.0 >At3g28770.1 68416.m03591 expressed protein Length = 2081 Score = 32.3 bits (70), Expect = 0.28 Identities = 27/110 (24%), Positives = 44/110 (40%) Frame = +1 Query: 304 HQDNKTFSTQSETHTKQSHTEHSSIKHVHSGTHKYITTSEDINNRTTSEDINDRTISQKR 483 H+DNK+ + + K+ H E S K K + ED N+ ED N++ SQ Sbjct: 1087 HEDNKSMKKEEDKKEKKKHEESKSRKKEED--KKDMEKLEDQNSNKKKEDKNEKKKSQHV 1144 Query: 484 PEFNIIFGFIERTRSPSVNDRNVVRSSTDRKIINKTDIKNVTNVSTSVKK 633 E+ + ++ + SS +K N+ D K + KK Sbjct: 1145 KLVKKESDKKEKKENEEKSETKEIESSKSQK--NEVDKKEKKSSKDQQKK 1192 >At1g28400.1 68414.m03492 expressed protein similar to E6 (GI:1000090) [Gossypium barbadense] Length = 335 Score = 31.5 bits (68), Expect = 0.49 Identities = 21/65 (32%), Positives = 32/65 (49%), Gaps = 3/65 (4%) Frame = +1 Query: 274 QFNNRKTTAIHQDNKTFSTQSETHTKQS---HTEHSSIKHVHSGTHKYITTSEDINNRTT 444 +FNN ++KTFST S + T++S + E+ K + GT Y +E+ NN Sbjct: 100 EFNNNNKNDEKVNSKTFSTPSLSETEESFNNYEENYPKKTENYGTKGY--NNEEFNNNNN 157 Query: 445 SEDIN 459 D N Sbjct: 158 KYDAN 162 >At5g61080.1 68418.m07664 expressed protein predicted proteins, Arabidopsis thaliana; expression supported by MPSS Length = 348 Score = 29.9 bits (64), Expect = 1.5 Identities = 16/57 (28%), Positives = 30/57 (52%) Frame = +2 Query: 35 TPGETPSQIRPKDNLRPEGDFDRPDTQKWVPSEKSGTFRPKDNITRNGEIIREDSYK 205 TP +P +++ + ++ P F++ T V GT P ++TR+ I+R+ S K Sbjct: 168 TPSRSPEKVKGEASVPP--GFEKIWTPPLVKLNIQGTSNPLSDLTRSAGIVRDQSGK 222 >At5g18860.1 68418.m02241 inosine-uridine preferring nucleoside hydrolase family protein contains Pfam profile PF01156: Inosine-uridine preferring nucleoside hydrolase Length = 890 Score = 29.1 bits (62), Expect = 2.6 Identities = 30/85 (35%), Positives = 39/85 (45%), Gaps = 9/85 (10%) Frame = +2 Query: 17 RSPTRYTPGETPSQIRPKDNLRPEGDFDRP-DTQKWVPSEKSGTFRPKDNITRNG----- 178 RSP RYT + + P+D RPE +P + W KSG K + NG Sbjct: 620 RSPRRYTAENSVTHGAPRDTDRPE--LRQPLAIEVWQNLTKSGNGVSKITVLTNGPLTNL 677 Query: 179 -EIIREDSYKSSEGVKK--IVGSKI 244 +II D KSS +K+ IVG I Sbjct: 678 AKIISSDK-KSSSLIKEVYIVGGHI 701 >At2g03630.1 68415.m00323 hypothetical protein Length = 252 Score = 29.1 bits (62), Expect = 2.6 Identities = 16/56 (28%), Positives = 28/56 (50%) Frame = +1 Query: 307 QDNKTFSTQSETHTKQSHTEHSSIKHVHSGTHKYITTSEDINNRTTSEDINDRTIS 474 Q+ +T SE+H++ S + SS S + Y++ +D+ E +ND IS Sbjct: 40 QETETVIINSESHSRLSSSSSSS-----SSSSSYLSPPKDLPEEVLKESLNDPEIS 90 >At3g03230.1 68416.m00319 esterase/lipase/thioesterase family protein contains Interpro entry IPR000379 Length = 333 Score = 28.3 bits (60), Expect = 4.6 Identities = 13/38 (34%), Positives = 19/38 (50%) Frame = +1 Query: 316 KTFSTQSETHTKQSHTEHSSIKHVHSGTHKYITTSEDI 429 K T+ + + HT+HS IK Y+TT +DI Sbjct: 215 KDTMTERDLELAEKHTKHSYIKESALRQGGYVTTQQDI 252 >At2g28640.1 68415.m03482 exocyst subunit EXO70 family protein contains HEAT repeat and Pfam domain PF03081:exocyst subunit EXO70 Length = 605 Score = 28.3 bits (60), Expect = 4.6 Identities = 17/61 (27%), Positives = 29/61 (47%) Frame = -2 Query: 190 SNDFSISSYIVFGPEGTRLFRWHPFLSIRTIKIAFRSQVIFRSNLRRCFTWSVSCRRTRS 11 S ++I ++ PE +L R H ++I ++ I +SN R SVS R + S Sbjct: 76 SLQYAIQGVVLVNPESPKLTRAHNLVTIAMKQLEKEFYRILKSNRRNLDPESVSVRSSPS 135 Query: 10 Y 8 + Sbjct: 136 F 136 >At4g10840.2 68417.m01764 kinesin light chain-related low similarity to kinesin light chain [Plectonema boryanum] GI:2645229; contains Pfam profile PF00515 TPR Domain Length = 531 Score = 27.9 bits (59), Expect = 6.1 Identities = 13/30 (43%), Positives = 17/30 (56%) Frame = +2 Query: 17 RSPTRYTPGETPSQIRPKDNLRPEGDFDRP 106 R+P + TP TPS+ +P N R G D P Sbjct: 34 RTPMKKTPSSTPSRSKPSPN-RSTGKKDSP 62 >At4g10840.1 68417.m01765 kinesin light chain-related low similarity to kinesin light chain [Plectonema boryanum] GI:2645229; contains Pfam profile PF00515 TPR Domain Length = 609 Score = 27.9 bits (59), Expect = 6.1 Identities = 13/30 (43%), Positives = 17/30 (56%) Frame = +2 Query: 17 RSPTRYTPGETPSQIRPKDNLRPEGDFDRP 106 R+P + TP TPS+ +P N R G D P Sbjct: 34 RTPMKKTPSSTPSRSKPSPN-RSTGKKDSP 62 >At2g03140.1 68415.m00267 CAAX amino terminal protease family protein very low similarity to SP|Q40863 Late embryogenesis abundant protein EMB8 from Picea glauca; contains Pfam profile PF02517 CAAX amino terminal protease family protein Length = 1805 Score = 27.9 bits (59), Expect = 6.1 Identities = 19/101 (18%), Positives = 42/101 (41%), Gaps = 3/101 (2%) Frame = +1 Query: 301 IHQDNKTFSTQSETHTKQSHTEHSSIKHVHSGTHKYITTSEDINNRTTSED---INDRTI 471 + Q + + Q + T ++ + + I + S + D N +T+ +D T Sbjct: 791 VEQGSGVATHQGQVETSKNDEKGAPIANEKSSVADGFEKASDAKNDSTNPQPVGADDITS 850 Query: 472 SQKRPEFNIIFGFIERTRSPSVNDRNVVRSSTDRKIINKTD 594 + + ++ +R S +D N +S+TD+ + TD Sbjct: 851 DGDKVDQGVVLAQQQRKDETSKSDENAKQSATDQNKVTSTD 891 >At2g34170.1 68415.m04182 expressed protein contains Pfam profile: PF05097 protein of unknown function (DUF688) Length = 523 Score = 27.5 bits (58), Expect = 8.0 Identities = 13/46 (28%), Positives = 21/46 (45%) Frame = +2 Query: 20 SPTRYTPGETPSQIRPKDNLRPEGDFDRPDTQKWVPSEKSGTFRPK 157 +PTRY TPS + D+ E D D + +++ G P+ Sbjct: 161 TPTRYESSFTPSYYQDIDDEESEEDSDDDEVSEYLSKRGCGMMSPQ 206 >At2g07360.1 68415.m00843 SH3 domain-containing protein contains Pfam profile PF00018: SH3 domain Length = 1196 Score = 27.5 bits (58), Expect = 8.0 Identities = 17/59 (28%), Positives = 25/59 (42%) Frame = +1 Query: 319 TFSTQSETHTKQSHTEHSSIKHVHSGTHKYITTSEDINNRTTSEDINDRTISQKRPEFN 495 T S+ S T ++ + SS H+H T E + R T I + TIS + N Sbjct: 25 TSSSASSTASQPTSASASSSSHLHHPMSTKTTLGEKKSKRATLMQIQNDTISVAKAALN 83 >At2g04620.1 68415.m00470 cation efflux family protein potential member of the cation diffusion facilitator (CDF) family, or cation efflux (CE) family, see PMID:11500563 Length = 798 Score = 27.5 bits (58), Expect = 8.0 Identities = 11/40 (27%), Positives = 20/40 (50%) Frame = +1 Query: 334 SETHTKQSHTEHSSIKHVHSGTHKYITTSEDINNRTTSED 453 S++H + H EH H HS H+ + D +++ S + Sbjct: 586 SDSHKHEEHHEHDHHHHSHSHKHEECNHNHDHEHQSHSHN 625 >At1g67650.1 68414.m07720 expressed protein Length = 651 Score = 27.5 bits (58), Expect = 8.0 Identities = 9/27 (33%), Positives = 16/27 (59%) Frame = +2 Query: 104 PDTQKWVPSEKSGTFRPKDNITRNGEI 184 PD ++W+P + ++RPK R +I Sbjct: 579 PDPERWLPRRERSSYRPKRKDKRAAQI 605 >At1g33980.1 68414.m04213 Smg-4/UPF3 family protein contains Pfam PF03467: Smg-4/UPF3 family; similar to hUPF3B (GI:12232324) [Homo sapiens] Length = 482 Score = 27.5 bits (58), Expect = 8.0 Identities = 16/47 (34%), Positives = 20/47 (42%), Gaps = 1/47 (2%) Frame = +2 Query: 98 DRPDTQKWVPSEKSGTFRPKDNITRNGEI-IREDSYKSSEGVKKIVG 235 DRPD W P +P + NGE+ R S +S E V G Sbjct: 380 DRPDRVMWAPRRDGSEDQPLSSAGNNGEVKDRMFSQRSGEVVNSSGG 426 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 11,802,860 Number of Sequences: 28952 Number of extensions: 219991 Number of successful extensions: 680 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 653 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 679 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1324661040 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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