BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30569 (649 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 113 3e-26 SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 113 3e-26 SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 72 8e-14 SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 55 1e-08 SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 50 4e-07 SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 45 1e-05 SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 42 1e-04 SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 36 0.005 SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 34 0.020 SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 34 0.020 SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 34 0.020 SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schi... 30 0.33 SPBC1271.15c |||translation initiation factor IF-2Mt|Schizosacch... 28 1.3 SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 26 4.1 SPCC1322.10 |||conserved fungal protein|Schizosaccharomyces pomb... 25 7.1 SPCC1235.05c |fft2||fun thirty related protein Fft2|Schizosaccha... 25 7.1 SPAP14E8.02 |||transcription factor |Schizosaccharomyces pombe|c... 25 7.1 SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 25 9.4 SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 25 9.4 SPBC1306.02 ||SPBC4.08|WD repeat protein, human WDR6 family|Schi... 25 9.4 >SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 842 Score = 113 bits (271), Expect = 3e-26 Identities = 53/74 (71%), Positives = 62/74 (83%) Frame = +1 Query: 34 MVNFTVDEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTR 213 MV FT +E+R +M K N+RNMSVIAHVDHGKSTLTDSLV KAGII+ A+AG+ RF DTR Sbjct: 1 MVAFTPEEVRNLMGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAGIISAAKAGDARFMDTR 60 Query: 214 KDEQDRCITIKSTA 255 DEQ+R +TIKSTA Sbjct: 61 ADEQERGVTIKSTA 74 Score = 89.8 bits (213), Expect = 3e-19 Identities = 48/86 (55%), Positives = 60/86 (69%), Gaps = 1/86 (1%) Frame = +3 Query: 255 ISMFFELEEKDLVFITNPDQREKSE-KGFLINLIDSPGHVDFSSEVTAALRVTDGALXXX 431 IS+F E+ + D+ D +E ++ FL+NLIDSPGHVDFSSEVTAALRVTDGAL Sbjct: 75 ISLFAEMTDDDM-----KDMKEPADGTDFLVNLIDSPGHVDFSSEVTAALRVTDGALVVV 129 Query: 432 XXXXXXXXQTETVLRQAIAERIKPIL 509 QTETVLRQA+ ERI+P++ Sbjct: 130 DTIEGVCVQTETVLRQALGERIRPVV 155 >SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elongation factor 2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 842 Score = 113 bits (271), Expect = 3e-26 Identities = 53/74 (71%), Positives = 62/74 (83%) Frame = +1 Query: 34 MVNFTVDEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTR 213 MV FT +E+R +M K N+RNMSVIAHVDHGKSTLTDSLV KAGII+ A+AG+ RF DTR Sbjct: 1 MVAFTPEEVRNLMGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAGIISAAKAGDARFMDTR 60 Query: 214 KDEQDRCITIKSTA 255 DEQ+R +TIKSTA Sbjct: 61 ADEQERGVTIKSTA 74 Score = 89.8 bits (213), Expect = 3e-19 Identities = 48/86 (55%), Positives = 60/86 (69%), Gaps = 1/86 (1%) Frame = +3 Query: 255 ISMFFELEEKDLVFITNPDQREKSE-KGFLINLIDSPGHVDFSSEVTAALRVTDGALXXX 431 IS+F E+ + D+ D +E ++ FL+NLIDSPGHVDFSSEVTAALRVTDGAL Sbjct: 75 ISLFAEMTDDDM-----KDMKEPADGTDFLVNLIDSPGHVDFSSEVTAALRVTDGALVVV 129 Query: 432 XXXXXXXXQTETVLRQAIAERIKPIL 509 QTETVLRQA+ ERI+P++ Sbjct: 130 DTIEGVCVQTETVLRQALGERIRPVV 155 >SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 1000 Score = 71.7 bits (168), Expect = 8e-14 Identities = 33/68 (48%), Positives = 48/68 (70%) Frame = +1 Query: 52 DEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDR 231 +++ + + NIRN +++AHVDHGK+TL DSL++ GII+ AG RF D R+DE R Sbjct: 7 EKLVSLQKNQENIRNFTLLAHVDHGKTTLADSLLASNGIISSKLAGTVRFLDFREDEITR 66 Query: 232 CITIKSTA 255 IT+KS+A Sbjct: 67 GITMKSSA 74 Score = 71.7 bits (168), Expect = 8e-14 Identities = 37/71 (52%), Positives = 47/71 (66%) Frame = +3 Query: 297 ITNPDQREKSEKGFLINLIDSPGHVDFSSEVTAALRVTDGALXXXXXXXXXXXQTETVLR 476 + + + ++ EK +LINLIDSPGHVDFSSEV++A R+ DGA QT TVLR Sbjct: 81 VISQNDEKRVEKDYLINLIDSPGHVDFSSEVSSASRLCDGAFVLVDAVEGVCSQTITVLR 140 Query: 477 QAIAERIKPIL 509 QA +RIK IL Sbjct: 141 QAWIDRIKVIL 151 >SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schizosaccharomyces pombe|chr 2|||Manual Length = 770 Score = 54.8 bits (126), Expect = 1e-08 Identities = 28/59 (47%), Positives = 35/59 (59%) Frame = +3 Query: 303 NPDQREKSEKGFLINLIDSPGHVDFSSEVTAALRVTDGALXXXXXXXXXXXQTETVLRQ 479 N Q+ EK + IN+ID+PGH+DF+ EV ALRV DGA+ QT TV RQ Sbjct: 134 NEKQKTDFEKSYNINIIDTPGHIDFTIEVERALRVLDGAVLVLCAVSGVQSQTITVDRQ 192 Score = 38.7 bits (86), Expect = 7e-04 Identities = 27/83 (32%), Positives = 43/83 (51%), Gaps = 7/83 (8%) Frame = +1 Query: 40 NFTVDEIRGMMDKKR--NIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGAR--AGETRF-- 201 N + E DKKR IRN+ + AH+D GK+T T+ ++ G I G+ Sbjct: 41 NLNIQEQLNDNDKKRLKQIRNIGISAHIDSGKTTFTERVLYYTGRIKDIHEVRGKDNVGA 100 Query: 202 -TDTRKDEQDRCITIKSTASLCS 267 D + E+++ ITI+S A+ C+ Sbjct: 101 KMDFMELEREKGITIQSAATHCT 123 >SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 646 Score = 49.6 bits (113), Expect = 4e-07 Identities = 28/70 (40%), Positives = 42/70 (60%) Frame = +1 Query: 58 IRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDRCI 237 +RG+ + +RN +VIAH+DHGKSTL+D ++ G+I +F D + E+ R I Sbjct: 50 VRGIPQNR--VRNWAVIAHIDHGKSTLSDCILKLTGVI-NEHNFRNQFLDKLEVERRRGI 106 Query: 238 TIKSTASLCS 267 T+K A CS Sbjct: 107 TVK--AQTCS 114 Score = 39.9 bits (89), Expect = 3e-04 Identities = 21/63 (33%), Positives = 34/63 (53%), Gaps = 2/63 (3%) Frame = +3 Query: 330 KGFLINLIDSPGHVDFSSEVTAALRVTDGALXXXXXXXXXXXQTETVLRQAIAER--IKP 503 + +L+NLID+PGHVDF +EV +L +G + QT + A ++ I P Sbjct: 122 QSYLLNLIDTPGHVDFRAEVMHSLAACEGCILLVDASQGIQAQTLSNFYMAFSQNLVIIP 181 Query: 504 ILS 512 +L+ Sbjct: 182 VLN 184 >SPBC660.10 |||translation elongation factor G|Schizosaccharomyces pombe|chr 2|||Manual Length = 813 Score = 44.8 bits (101), Expect = 1e-05 Identities = 22/56 (39%), Positives = 31/56 (55%) Frame = +3 Query: 342 INLIDSPGHVDFSSEVTAALRVTDGALXXXXXXXXXXXQTETVLRQAIAERIKPIL 509 INLID+PGH DF+ EV ++ V DGA+ QT+ V +QA I ++ Sbjct: 95 INLIDTPGHADFTFEVERSVAVLDGAVAIIDGSAGVEAQTKVVWKQATKRGIPKVI 150 Score = 39.1 bits (87), Expect = 5e-04 Identities = 23/60 (38%), Positives = 33/60 (55%), Gaps = 3/60 (5%) Frame = +1 Query: 85 NIRNMSVIAHVDHGKSTLTDSLVSKAGI---IAGARAGETRFTDTRKDEQDRCITIKSTA 255 +IRN+ +IAH+D GK+TLT+ ++ G G+T D E+ R ITI S A Sbjct: 27 SIRNVGIIAHIDAGKTTLTEKMLYYGGFTSHFGNVDTGDT-VMDYLPAERQRGITINSAA 85 >SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces pombe|chr 2|||Manual Length = 983 Score = 41.5 bits (93), Expect = 1e-04 Identities = 23/74 (31%), Positives = 33/74 (44%) Frame = +3 Query: 330 KGFLINLIDSPGHVDFSSEVTAALRVTDGALXXXXXXXXXXXQTETVLRQAIAERIKPIL 509 K F ID+PGHVDF EV A + ++DG + T +++ AI + +L Sbjct: 207 KTFAFQCIDTPGHVDFVDEVAAPMAISDGVVLVVDVIEGVMINTTRIIKHAILHDMPIVL 266 Query: 510 S*TKWTVLFLSSNL 551 K L L L Sbjct: 267 VLNKVDRLILELRL 280 Score = 39.9 bits (89), Expect = 3e-04 Identities = 26/76 (34%), Positives = 40/76 (52%), Gaps = 2/76 (2%) Frame = +1 Query: 64 GMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGI-IAGARAGETRFTDTRKDEQDRCIT 240 G++ ++R+ V H+ HGKS L D LV + R+TDT E++R ++ Sbjct: 132 GLLTGTDDVRSFIVAGHLHHGKSALLDLLVYYTHPDTKPPKRRSLRYTDTHYLERERVMS 191 Query: 241 IKST-ASLCSSSLKRK 285 IKST +L S +K K Sbjct: 192 IKSTPLTLAVSDMKGK 207 >SPBC9B6.04c |tuf1||mitochondrial translation elongation factor EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 439 Score = 35.9 bits (79), Expect = 0.005 Identities = 21/58 (36%), Positives = 31/58 (53%) Frame = +1 Query: 76 KKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDRCITIKS 249 KK ++ N+ I HVDHGK+TLT ++ + A + D +E+ R ITI S Sbjct: 50 KKPHV-NIGTIGHVDHGKTTLTAAITKCLSDLGQASFMDYSQIDKAPEEKARGITISS 106 Score = 25.8 bits (54), Expect = 5.4 Identities = 14/53 (26%), Positives = 20/53 (37%) Frame = +3 Query: 351 IDSPGHVDFSSEVTAALRVTDGALXXXXXXXXXXXQTETVLRQAIAERIKPIL 509 +D PGH D+ + DGA+ QT L A +K I+ Sbjct: 121 VDCPGHADYIKNMITGAATMDGAIIVVSATDGQMPQTREHLLLARQVGVKQIV 173 >SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual Length = 460 Score = 33.9 bits (74), Expect = 0.020 Identities = 16/32 (50%), Positives = 20/32 (62%) Frame = +1 Query: 70 MDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAG 165 M K++ N+ VI HVD GKST T L+ K G Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCG 32 >SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual Length = 460 Score = 33.9 bits (74), Expect = 0.020 Identities = 16/32 (50%), Positives = 20/32 (62%) Frame = +1 Query: 70 MDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAG 165 M K++ N+ VI HVD GKST T L+ K G Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCG 32 >SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual Length = 460 Score = 33.9 bits (74), Expect = 0.020 Identities = 16/32 (50%), Positives = 20/32 (62%) Frame = +1 Query: 70 MDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAG 165 M K++ N+ VI HVD GKST T L+ K G Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCG 32 >SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 374 Score = 29.9 bits (64), Expect = 0.33 Identities = 22/75 (29%), Positives = 36/75 (48%), Gaps = 1/75 (1%) Frame = -1 Query: 472 STVSVCTHTPDTQSTT-TRAPSVTRSAAVTSEEKSTCPGESIKLIKKPFSLFSRWSGFVM 296 +T S + +P + STT T +PS + S++ +S S+ S S S S Sbjct: 135 TTSSSSSSSPSSSSTTTTTSPSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSS 194 Query: 295 NTKSFSSSSKNIEMP 251 ++ S SSSS + +P Sbjct: 195 SSSSSSSSSSSSSVP 209 Score = 27.1 bits (57), Expect = 2.3 Identities = 19/71 (26%), Positives = 33/71 (46%) Frame = -1 Query: 475 RSTVSVCTHTPDTQSTTTRAPSVTRSAAVTSEEKSTCPGESIKLIKKPFSLFSRWSGFVM 296 ++TVS + + T S+++ +PS + + TS S+ S S S S Sbjct: 124 QTTVSSSSVSSTTSSSSSSSPSSSSTTTTTSPSSSSSSSSSSSSSSSSSSSSSSSSSSSS 183 Query: 295 NTKSFSSSSKN 263 ++ S SSSS + Sbjct: 184 SSSSSSSSSSS 194 >SPBC1271.15c |||translation initiation factor IF-2Mt|Schizosaccharomyces pombe|chr 2|||Manual Length = 686 Score = 27.9 bits (59), Expect = 1.3 Identities = 13/34 (38%), Positives = 22/34 (64%) Frame = +1 Query: 97 MSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETR 198 ++++ HVDHGK+TL D+ K+ I + G T+ Sbjct: 174 VTLMGHVDHGKTTLLDAF-RKSTIASTEHGGITQ 206 >SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 662 Score = 26.2 bits (55), Expect = 4.1 Identities = 11/26 (42%), Positives = 17/26 (65%) Frame = +1 Query: 94 NMSVIAHVDHGKSTLTDSLVSKAGII 171 N+ I HVD GKSTL +++ G++ Sbjct: 240 NIVFIGHVDAGKSTLGGNILFLTGMV 265 >SPCC1322.10 |||conserved fungal protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 262 Score = 25.4 bits (53), Expect = 7.1 Identities = 19/70 (27%), Positives = 33/70 (47%) Frame = -1 Query: 472 STVSVCTHTPDTQSTTTRAPSVTRSAAVTSEEKSTCPGESIKLIKKPFSLFSRWSGFVMN 293 S+ S T TP + STT+ + S + S ++S S+ + + S S S + Sbjct: 140 SSTSSSTATPSSSSTTSSSSSSSSSTPISSSITSSISSSASSSVSSS-SASSSGSISSAD 198 Query: 292 TKSFSSSSKN 263 K+ S+SS + Sbjct: 199 AKTVSASSNS 208 >SPCC1235.05c |fft2||fun thirty related protein Fft2|Schizosaccharomyces pombe|chr 3|||Manual Length = 1284 Score = 25.4 bits (53), Expect = 7.1 Identities = 12/28 (42%), Positives = 16/28 (57%) Frame = -1 Query: 85 SASCPSSHGSRLP*NSPF*MVYDFCSIK 2 +ASCP SH L + PF + + C IK Sbjct: 417 TASCPLSHSKLLLEHRPFQTLAEACIIK 444 >SPAP14E8.02 |||transcription factor |Schizosaccharomyces pombe|chr 1|||Manual Length = 566 Score = 25.4 bits (53), Expect = 7.1 Identities = 13/37 (35%), Positives = 21/37 (56%), Gaps = 1/37 (2%) Frame = +2 Query: 119 ITASQPSRTRWFPR-PVSLLVREPERPVSLTRVRTNK 226 +T S T + P P S + REP P+S R+R+++ Sbjct: 48 LTPEPSSNTFYAPSSPASAVRREPLSPMSFVRMRSHR 84 >SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma subunit|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 25.0 bits (52), Expect = 9.4 Identities = 9/19 (47%), Positives = 13/19 (68%) Frame = +1 Query: 94 NMSVIAHVDHGKSTLTDSL 150 N+ I HV HGKST+ ++ Sbjct: 25 NIGTIGHVAHGKSTVVKAI 43 >SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 592 Score = 25.0 bits (52), Expect = 9.4 Identities = 17/53 (32%), Positives = 28/53 (52%) Frame = +1 Query: 13 KNHKPSKMVNFTVDEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGII 171 K P+ +V+ V EI + K + ++ V HVD GKST+ ++ + G I Sbjct: 155 KKQNPTDLVS--VPEIFEQSNPKPVV-HLVVTGHVDSGKSTMLGRIMFELGEI 204 >SPBC1306.02 ||SPBC4.08|WD repeat protein, human WDR6 family|Schizosaccharomyces pombe|chr 2|||Manual Length = 984 Score = 25.0 bits (52), Expect = 9.4 Identities = 11/41 (26%), Positives = 21/41 (51%) Frame = +1 Query: 385 K*QLHSVSLMEPLWLLTVCLVCVYKPKQYCVRLLPSASSLF 507 K Q+H +SL + W +T + C P +C + S + ++ Sbjct: 120 KNQVHLLSLSKGSWEVTNTISCEKTPLLFCATVQISGNEIY 160 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,638,049 Number of Sequences: 5004 Number of extensions: 52034 Number of successful extensions: 186 Number of sequences better than 10.0: 20 Number of HSP's better than 10.0 without gapping: 172 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 186 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 291768710 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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