Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30552
(777 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC8E4.04 |||aldo/keto reductase involved in pentose catabolism... 58 1e-09
SPAC26F1.07 |||2-methylbutyraldehyde reductase |Schizosaccharomy... 56 6e-09
SPAC19G12.09 |||NADH/NADPH dependent indole-3-acetaldehyde reduc... 55 1e-08
SPAC2F3.05c |||xylose and arabinose reductase |Schizosaccharomyc... 42 7e-05
SPBC28F2.05c |||xylose and arabinose reductase |Schizosaccharomy... 38 0.001
SPAP32A8.02 |||xylose and arabinose reductase |Schizosaccharomyc... 37 0.004
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 31 0.14
SPCC965.06 |||potassium channel subunit |Schizosaccharomyces pom... 30 0.43
SPBC21D10.05c |ucp3|soc2|GTPase activating protein Ucp3 |Schizos... 27 2.3
SPBC32H8.13c |mok12||alpha-1,3-glucan synthase Mok12|Schizosacch... 27 2.3
SPAC607.08c |||DUF726 family protein|Schizosaccharomyces pombe|c... 26 5.2
SPBC1652.02 |aap1|SPBC16A3.20c|APC amino acid transporter|Schizo... 26 6.9
SPAC29B12.01 |ino80|SPAC3G6.12|SNF2 family helicase Ino80|Schizo... 26 6.9
SPAC3F10.16c |||GTP binding protein, HSR1-related|Schizosaccharo... 26 6.9
>SPBC8E4.04 |||aldo/keto reductase involved in pentose catabolism
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 325
Score = 58.0 bits (134), Expect = 1e-09
Identities = 38/95 (40%), Positives = 51/95 (53%), Gaps = 12/95 (12%)
Frame = +2
Query: 260 QIGAAVRASGIPREDIFIVTKLPMDNQRD--VVKQLEKSLARLNMSYVDLYLIHNPVAFN 433
+IG +R SG+PR DI++ +KL + R V LEK+L LN+ Y+D YLIH P A
Sbjct: 64 EIGEGIRESGVPRTDIWVTSKLWCNAHRAGLVPLALEKTLQDLNLEYIDAYLIHWPFALL 123
Query: 434 PG-------FKG---YDIVDYLDTWKGMEEAKNLG 508
G KG Y+ V +TW+ MEE G
Sbjct: 124 SGPEELPRNEKGELIYEDVPIEETWQAMEELLETG 158
Score = 41.5 bits (93), Expect = 1e-04
Identities = 17/44 (38%), Positives = 32/44 (72%)
Frame = +1
Query: 520 IGISNFNTTQIDRILENGQIKPSVLQVEVNLHLGQDKLIDYCKR 651
IGISNFN +DR+L+ ++KP++ Q+E++ +L Q + ++ K+
Sbjct: 163 IGISNFNNEYLDRVLKIAKVKPTIHQMELHPYLPQTEYLEKHKK 206
Score = 36.7 bits (81), Expect = 0.004
Identities = 24/56 (42%), Positives = 34/56 (60%)
Frame = +3
Query: 90 SLNDGNSIPALGLGTFLGFDEKGQKEVKPGDSEKPVKWALEAGYRLIDTAALYLNK 257
+L +G+ IP++GLGT+ G+ E K V AL+AGYR IDTA +Y N+
Sbjct: 16 TLPNGDKIPSIGLGTW----RSGKDETK-----NAVCAALKAGYRHIDTAHIYGNE 62
>SPAC26F1.07 |||2-methylbutyraldehyde reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 321
Score = 56.0 bits (129), Expect = 6e-09
Identities = 27/63 (42%), Positives = 41/63 (65%), Gaps = 2/63 (3%)
Frame = +2
Query: 257 DQIGAAVRASGIPREDIFIVTKLPMDNQRD--VVKQLEKSLARLNMSYVDLYLIHNPVAF 430
D++G ++ SG+PR+DI++ +KL + V K LEK+L L + Y+D YLIH PV+F
Sbjct: 64 DEVGDGIKESGVPRKDIWVTSKLWCNAHAPEAVPKALEKTLKDLKLDYLDEYLIHWPVSF 123
Query: 431 NPG 439
G
Sbjct: 124 KTG 126
Score = 48.4 bits (110), Expect = 1e-06
Identities = 24/59 (40%), Positives = 40/59 (67%)
Frame = +1
Query: 514 KSIGISNFNTTQIDRILENGQIKPSVLQVEVNLHLGQDKLIDYCKRNGIVC*WRFSPFG 690
+ IG+SNFN T ++RIL+ ++KP+V Q+E++ L Q + ++ K+ GI +SPFG
Sbjct: 162 RHIGLSNFNDTNLERILKVAKVKPAVHQMELHPFLPQTEFVEKHKKLGIHV-TAYSPFG 219
Score = 41.5 bits (93), Expect = 1e-04
Identities = 24/56 (42%), Positives = 33/56 (58%)
Frame = +3
Query: 90 SLNDGNSIPALGLGTFLGFDEKGQKEVKPGDSEKPVKWALEAGYRLIDTAALYLNK 257
+L DG+ IP LGLGT+ +P ++ VK AL+ GYR ID AA+Y N+
Sbjct: 17 TLADGSKIPGLGLGTWRS---------EPNQTKNAVKTALQYGYRHIDAAAIYGNE 63
>SPAC19G12.09 |||NADH/NADPH dependent indole-3-acetaldehyde
reductase AKR3C2|Schizosaccharomyces pombe|chr
1|||Manual
Length = 284
Score = 55.2 bits (127), Expect = 1e-08
Identities = 27/84 (32%), Positives = 48/84 (57%)
Frame = +2
Query: 257 DQIGAAVRASGIPREDIFIVTKLPMDNQRDVVKQLEKSLARLNMSYVDLYLIHNPVAFNP 436
+++G A++ + +PR +FI +K+ M N ++ + L +SL +L Y+DLYL+H+P+ F
Sbjct: 58 EEVGVALKEANVPRSKLFITSKV-MHNVDNIPEALNESLRKLGTDYLDLYLLHSPIPFYE 116
Query: 437 GFKGYDIVDYLDTWKGMEEAKNLG 508
+ + WK ME A G
Sbjct: 117 -----KKIPISEGWKAMETALGTG 135
Score = 39.5 bits (88), Expect = 5e-04
Identities = 19/67 (28%), Positives = 36/67 (53%), Gaps = 2/67 (2%)
Frame = +1
Query: 508 LAKSIGISNFNTTQIDRILENGQIKPSVLQVEVN--LHLGQDKLIDYCKRNGIVC*WRFS 681
L S+G+SNF ++ +L+ I P V Q+E + ++ L+++C+ GI+
Sbjct: 136 LVHSVGVSNFRIPDLEELLKTSTITPRVNQIEFHPQVYKAAKPLVEFCQSKGII----VE 191
Query: 682 PFGPMFP 702
+GP+ P
Sbjct: 192 GYGPLSP 198
Score = 31.5 bits (68), Expect = 0.14
Identities = 22/49 (44%), Positives = 28/49 (57%)
Frame = +3
Query: 111 IPALGLGTFLGFDEKGQKEVKPGDSEKPVKWALEAGYRLIDTAALYLNK 257
+PA G+GT L EKG+ DS VK AL AG+ ID A +Y N+
Sbjct: 12 VPAYGVGTALFKKEKGEINRTIVDS---VKNALAAGFIHIDCAEVYGNE 57
>SPAC2F3.05c |||xylose and arabinose reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 275
Score = 42.3 bits (95), Expect = 7e-05
Identities = 21/58 (36%), Positives = 37/58 (63%), Gaps = 1/58 (1%)
Frame = +1
Query: 490 GSKKSRLAKSIGISNFNTTQIDRILENG-QIKPSVLQVEVNLHLGQDKLIDYCKRNGI 660
G ++ +L ++IG+SNF I +L++ +I P V Q+E++ Q K++DYC+ GI
Sbjct: 127 GVEEGKL-RAIGVSNFGPHHIQELLDSHPKIIPCVNQIELHPFCSQQKVVDYCESKGI 183
Score = 38.3 bits (85), Expect = 0.001
Identities = 22/89 (24%), Positives = 41/89 (46%), Gaps = 1/89 (1%)
Frame = +2
Query: 284 SGIPREDIFIVTKL-PMDNQRDVVKQLEKSLARLNMSYVDLYLIHNPVAFNPGFKGYDIV 460
+G REDI+ +KL + + + ++ S+ + Y+DL+L+H+P
Sbjct: 67 TGTKREDIWFTSKLNDLSGYKSTLSSIDASVKACGLGYIDLFLLHSPYG----------- 115
Query: 461 DYLDTWKGMEEAKNLG*LNL*AYLTSTPH 547
D +++WK +E+ G L PH
Sbjct: 116 DRIESWKALEKGVEEGKLRAIGVSNFGPH 144
>SPBC28F2.05c |||xylose and arabinose reductase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 276
Score = 38.3 bits (85), Expect = 0.001
Identities = 21/63 (33%), Positives = 35/63 (55%)
Frame = +1
Query: 496 KKSRLAKSIGISNFNTTQIDRILENGQIKPSVLQVEVNLHLGQDKLIDYCKRNGIVC*WR 675
K+ R+ K IG+SN+N ++ I+ G P++ QVE + + + YC +GI+
Sbjct: 130 KEGRINK-IGVSNYNIHHLEEIISLGLPLPAINQVEFSAFNNRPTFLSYCFNHGILV-QA 187
Query: 676 FSP 684
FSP
Sbjct: 188 FSP 190
Score = 34.3 bits (75), Expect = 0.020
Identities = 18/46 (39%), Positives = 27/46 (58%), Gaps = 2/46 (4%)
Frame = +2
Query: 290 IPREDIFIVTKLP--MDNQRDVVKQLEKSLARLNMSYVDLYLIHNP 421
I REDI ++TK+P + K +E+SL R +D+ LIH+P
Sbjct: 67 IKREDITLITKIPDSLQGFERTWKAVEQSLRRTGRPKLDVVLIHSP 112
>SPAP32A8.02 |||xylose and arabinose reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 283
Score = 36.7 bits (81), Expect = 0.004
Identities = 15/50 (30%), Positives = 29/50 (58%), Gaps = 1/50 (2%)
Frame = +1
Query: 514 KSIGISNFNTTQIDRIL-ENGQIKPSVLQVEVNLHLGQDKLIDYCKRNGI 660
+S+G+SN+ + + N + P V Q+E++ L QD ++ YC+ + I
Sbjct: 141 RSVGVSNYGVKHLQELYASNPKFYPCVNQIELHPFLSQDDIVKYCQSHDI 190
Score = 33.9 bits (74), Expect = 0.026
Identities = 21/57 (36%), Positives = 32/57 (56%)
Frame = +3
Query: 87 ISLNDGNSIPALGLGTFLGFDEKGQKEVKPGDSEKPVKWALEAGYRLIDTAALYLNK 257
++L +G IP +G G F+ +K + V AL++GYR IDTAA+Y N+
Sbjct: 11 VTLTNGMVIPRIGFGAFM---------LKYNECYGLVTQALDSGYRHIDTAAVYGNE 58
>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 857
Score = 31.5 bits (68), Expect = 0.14
Identities = 19/62 (30%), Positives = 29/62 (46%), Gaps = 3/62 (4%)
Frame = -3
Query: 220 YPASKAHLTGFSESPGLTSFC---PFSSNPRKVPRPKAGIELPSFSEIFGALPPPVVASE 50
+P + A ++ F+ G TSF PF++NP + P E P+ E PPP +
Sbjct: 437 FPKTAAKISSFNSKAG-TSFAKPRPFTNNPNPISAP----EKPTSGESLSLNPPPAMPKV 491
Query: 49 IP 44
P
Sbjct: 492 FP 493
>SPCC965.06 |||potassium channel subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 344
Score = 29.9 bits (64), Expect = 0.43
Identities = 14/38 (36%), Positives = 23/38 (60%), Gaps = 3/38 (7%)
Frame = +2
Query: 317 TKLPMD---NQRDVVKQLEKSLARLNMSYVDLYLIHNP 421
TKLP +++ +++ L SL RL + YVD+ + H P
Sbjct: 102 TKLPNTTGLSRKHIIEGLNASLKRLGLPYVDVIMAHRP 139
>SPBC21D10.05c |ucp3|soc2|GTPase activating protein Ucp3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 601
Score = 27.5 bits (58), Expect = 2.3
Identities = 12/19 (63%), Positives = 14/19 (73%)
Frame = +3
Query: 684 VWPDVSTTASPFNTAPNDP 740
V P V TT+SPF TAP +P
Sbjct: 272 VSPGVETTSSPFFTAPVEP 290
>SPBC32H8.13c |mok12||alpha-1,3-glucan synthase
Mok12|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2352
Score = 27.5 bits (58), Expect = 2.3
Identities = 11/22 (50%), Positives = 17/22 (77%)
Frame = +2
Query: 323 LPMDNQRDVVKQLEKSLARLNM 388
LP+DN +++KQL +S RLN+
Sbjct: 1151 LPLDNSSELLKQLPESEKRLNV 1172
>SPAC607.08c |||DUF726 family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 579
Score = 26.2 bits (55), Expect = 5.2
Identities = 12/27 (44%), Positives = 15/27 (55%)
Frame = +3
Query: 63 TGGGKAPKISLNDGNSIPALGLGTFLG 143
TGG AP ++ G LGLGT +G
Sbjct: 189 TGGLAAPFVAAGLGTLFAGLGLGTMIG 215
>SPBC1652.02 |aap1|SPBC16A3.20c|APC amino acid
transporter|Schizosaccharomyces pombe|chr 2|||Manual
Length = 594
Score = 25.8 bits (54), Expect = 6.9
Identities = 11/39 (28%), Positives = 22/39 (56%)
Frame = +2
Query: 392 YVDLYLIHNPVAFNPGFKGYDIVDYLDTWKGMEEAKNLG 508
Y+ + H+P +F GFKG+ V ++ + + +N+G
Sbjct: 240 YIGAHYWHHPGSFRNGFKGFCSV-FISSAYSLSGTENIG 277
>SPAC29B12.01 |ino80|SPAC3G6.12|SNF2 family helicase
Ino80|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1604
Score = 25.8 bits (54), Expect = 6.9
Identities = 13/30 (43%), Positives = 15/30 (50%)
Frame = -3
Query: 133 VPRPKAGIELPSFSEIFGALPPPVVASEIP 44
VPRP A P + +LP P ASE P
Sbjct: 172 VPRPDANTVSPHAARSASSLPVPKEASETP 201
>SPAC3F10.16c |||GTP binding protein,
HSR1-related|Schizosaccharomyces pombe|chr 1|||Manual
Length = 616
Score = 25.8 bits (54), Expect = 6.9
Identities = 14/36 (38%), Positives = 22/36 (61%), Gaps = 5/36 (13%)
Frame = -2
Query: 155 FLVEPEEGAETQ-----SRDRVTIVQRNLWSFTTTS 63
FL+ EE A ++ ++DR+TI +R W TTT+
Sbjct: 87 FLLSKEEAARSKQKQEKNKDRLTIPRRPHWDQTTTA 122
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,363,649
Number of Sequences: 5004
Number of extensions: 72096
Number of successful extensions: 236
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 207
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 229
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 375345278
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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