BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30552 (777 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_56309| Best HMM Match : No HMM Matches (HMM E-Value=.) 55 6e-08 SB_54717| Best HMM Match : Aldo_ket_red (HMM E-Value=0) 54 1e-07 SB_8916| Best HMM Match : No HMM Matches (HMM E-Value=.) 54 1e-07 SB_34622| Best HMM Match : No HMM Matches (HMM E-Value=.) 50 2e-06 SB_7528| Best HMM Match : Aldo_ket_red (HMM E-Value=0) 48 6e-06 SB_33699| Best HMM Match : Aldo_ket_red (HMM E-Value=3.1) 46 3e-05 SB_4480| Best HMM Match : TAT_ubiq (HMM E-Value=8.2) 32 0.60 SB_39733| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 3.2 SB_3196| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 3.2 SB_57427| Best HMM Match : Exo_endo_phos (HMM E-Value=0.00044) 29 5.5 SB_55921| Best HMM Match : Aldo_ket_red (HMM E-Value=0.16) 28 7.3 SB_49185| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 7.3 SB_16955| Best HMM Match : SLAP (HMM E-Value=0.048) 28 7.3 SB_1933| Best HMM Match : PI3_PI4_kinase (HMM E-Value=4.06377e-44) 28 9.7 >SB_56309| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 394 Score = 55.2 bits (127), Expect = 6e-08 Identities = 24/56 (42%), Positives = 40/56 (71%), Gaps = 2/56 (3%) Frame = +2 Query: 260 QIGAAVRASGIPREDIFIVTKL--PMDNQRDVVKQLEKSLARLNMSYVDLYLIHNP 421 ++G+AVR SG+ REDI++ TKL + + +K ++S+ +L++ YVDL+LIH P Sbjct: 72 EVGSAVRKSGLKREDIYVTTKLKPSEEGHSNALKYAKESIKKLDIGYVDLFLIHTP 127 Score = 46.4 bits (105), Expect = 3e-05 Identities = 18/55 (32%), Positives = 35/55 (63%) Frame = +1 Query: 496 KKSRLAKSIGISNFNTTQIDRILENGQIKPSVLQVEVNLHLGQDKLIDYCKRNGI 660 K+ L +S+G+SNF ++ + + G P++ Q+E+N Q+++I YC ++GI Sbjct: 143 KEEGLIRSVGVSNFGVHHLEELRKAGCRTPAINQIEINPFWRQEEIIKYCNKHGI 197 >SB_54717| Best HMM Match : Aldo_ket_red (HMM E-Value=0) Length = 333 Score = 54.0 bits (124), Expect = 1e-07 Identities = 27/56 (48%), Positives = 36/56 (64%), Gaps = 2/56 (3%) Frame = +2 Query: 263 IGAAVRASGIPREDIFIVTKLPMDNQ--RDVVKQLEKSLARLNMSYVDLYLIHNPV 424 +G AVR S IPR +I++VTK+ ++ + KSL RL + YVDLYLIH PV Sbjct: 115 VGEAVRCSNIPRCEIYVVTKVYFTEHGYKETMDAFYKSLTRLGLGYVDLYLIHFPV 170 Score = 46.4 bits (105), Expect = 3e-05 Identities = 23/59 (38%), Positives = 35/59 (59%) Frame = +1 Query: 508 LAKSIGISNFNTTQIDRILENGQIKPSVLQVEVNLHLGQDKLIDYCKRNGIVC*WRFSP 684 L +SIG+SNFN ++ + + + P+V Q+EV+ L +L YCKR+ I FSP Sbjct: 189 LIRSIGVSNFNIQHLEALRQQTGVVPAVNQIEVHPFLQMKELTQYCKRHNITI-QAFSP 246 Score = 41.9 bits (94), Expect = 6e-04 Identities = 25/55 (45%), Positives = 33/55 (60%) Frame = +3 Query: 93 LNDGNSIPALGLGTFLGFDEKGQKEVKPGDSEKPVKWALEAGYRLIDTAALYLNK 257 +NDG IP LGLG ++ S++ V WALE+GYRLIDTA+ Y N+ Sbjct: 70 MNDGRKIPLLGLGAH---------DIH---SKQAVLWALESGYRLIDTASRYHNE 112 >SB_8916| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 619 Score = 54.0 bits (124), Expect = 1e-07 Identities = 32/88 (36%), Positives = 47/88 (53%), Gaps = 5/88 (5%) Frame = +2 Query: 260 QIGAAVRASGIPREDIFIVTKLPMD--NQRDVVKQLEKSLARLNMSYVDLYLIHNPVAFN 433 Q+ A+ SGIPR+DIFI+TKL +K +E SL L+ Y+DL+LIH+ + Sbjct: 378 QVAKAIADSGIPRKDIFIITKLHPRYLGYEPTLKSVEMSLRALSTDYIDLFLIHSKTCDD 437 Query: 434 ---PGFKGYDIVDYLDTWKGMEEAKNLG 508 +G + ++WK MEE G Sbjct: 438 FLLTCAEGEPRGTWKESWKAMEELHRQG 465 Score = 30.3 bits (65), Expect = 1.8 Identities = 15/23 (65%), Positives = 16/23 (69%) Frame = +3 Query: 180 DSEKPVKWALEAGYRLIDTAALY 248 D+ VK ALE GYRLIDTA Y Sbjct: 350 DTLSVVKTALETGYRLIDTAQGY 372 >SB_34622| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 340 Score = 50.4 bits (115), Expect = 2e-06 Identities = 28/63 (44%), Positives = 39/63 (61%) Frame = +1 Query: 508 LAKSIGISNFNTTQIDRILENGQIKPSVLQVEVNLHLGQDKLIDYCKRNGIVC*WRFSPF 687 L K+IG+SNFN+ Q+D I +IKP QVE + L Q KLID+ K++ I +SP Sbjct: 151 LVKAIGLSNFNSKQVDDICAIARIKPVANQVECHPLLNQAKLIDHHKKHNIQT-IAYSPL 209 Query: 688 GPM 696 G + Sbjct: 210 GSL 212 Score = 48.8 bits (111), Expect = 5e-06 Identities = 35/98 (35%), Positives = 50/98 (51%), Gaps = 15/98 (15%) Frame = +2 Query: 260 QIGAAVR---ASGIPREDIFIVTKL--PMDNQRDVVKQLEKSLARLNMSYVDLYLIHNPV 424 ++G A+R S + R+D+FI +KL N +DV SL L + Y+DLYLIH P+ Sbjct: 53 EVGDALRELLGSVVERKDLFITSKLWNTKHNPKDVRSNCVDSLKDLGLDYLDLYLIHWPL 112 Query: 425 AFNPGFKG----------YDIVDYLDTWKGMEEAKNLG 508 +F G + Y D DTWK ME+ + G Sbjct: 113 SFRDGDEFFPKDEQGNVLYAYHDPCDTWKAMEKLVDEG 150 Score = 42.7 bits (96), Expect = 3e-04 Identities = 24/57 (42%), Positives = 33/57 (57%) Frame = +3 Query: 87 ISLNDGNSIPALGLGTFLGFDEKGQKEVKPGDSEKPVKWALEAGYRLIDTAALYLNK 257 + LN+G +P LGLGT+ + KPG E VK+A++ GYR ID A Y N+ Sbjct: 4 VKLNNGLEMPVLGLGTW---------KSKPGQVENAVKFAIKEGYRHIDCAYAYRNE 51 >SB_7528| Best HMM Match : Aldo_ket_red (HMM E-Value=0) Length = 407 Score = 48.4 bits (110), Expect = 6e-06 Identities = 28/79 (35%), Positives = 42/79 (53%), Gaps = 12/79 (15%) Frame = +2 Query: 290 IPREDIFIVTKLPMDNQR--DVVKQLEKSLARLNMSYVDLYLIHNPVAFNPGFK------ 445 + RE++F+ +KL D+ DV+ + +L L + Y+DLYLIH PVAF G + Sbjct: 107 VKREELFVTSKLWCDSHHPDDVLPACQATLKNLQLDYLDLYLIHIPVAFKKGVRLPHSIA 166 Query: 446 ----GYDIVDYLDTWKGME 490 GY +TW+ ME Sbjct: 167 EGIIGYTPEGVQNTWQAME 185 Score = 48.4 bits (110), Expect = 6e-06 Identities = 20/50 (40%), Positives = 35/50 (70%) Frame = +1 Query: 508 LAKSIGISNFNTTQIDRILENGQIKPSVLQVEVNLHLGQDKLIDYCKRNG 657 L K+IG+SNF+ +++++LE I P+ QVE++ +L Q+KL ++C G Sbjct: 192 LCKAIGVSNFSVKRLNKLLETASIVPACNQVELHPYLPQEKLKEFCDSKG 241 >SB_33699| Best HMM Match : Aldo_ket_red (HMM E-Value=3.1) Length = 93 Score = 46.4 bits (105), Expect = 3e-05 Identities = 28/58 (48%), Positives = 34/58 (58%) Frame = +3 Query: 90 SLNDGNSIPALGLGTFLGFDEKGQKEVKPGDSEKPVKWALEAGYRLIDTAALYLNKIR 263 +L+DG IP GLG + DEK K+ V WALE GYR+IDTAA Y N+ R Sbjct: 2 TLSDGYKIPRFGLGLY-DLDEKHTKQA--------VLWALENGYRMIDTAASYNNEKR 50 Score = 29.5 bits (63), Expect = 3.2 Identities = 13/43 (30%), Positives = 27/43 (62%), Gaps = 2/43 (4%) Frame = +2 Query: 260 QIGAAVRASGIPREDIFIVTKLPMDNQ--RDVVKQLEKSLARL 382 ++G A+R S +PR ++++VTK+ + +K ++SL+ L Sbjct: 50 RVGEALRESAVPRSEVYLVTKVYHTDHGYEKTMKAYDRSLSAL 92 >SB_4480| Best HMM Match : TAT_ubiq (HMM E-Value=8.2) Length = 107 Score = 31.9 bits (69), Expect = 0.60 Identities = 15/38 (39%), Positives = 23/38 (60%) Frame = +1 Query: 469 GHLERHGGSKKSRLAKSIGISNFNTTQIDRILENGQIK 582 G LE H G +SR + S G+ F+ I+RIL++ I+ Sbjct: 1 GELELHRGGGRSRTSGSPGLQEFDDEDINRILKHVNIR 38 >SB_39733| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 2839 Score = 29.5 bits (63), Expect = 3.2 Identities = 25/82 (30%), Positives = 38/82 (46%), Gaps = 1/82 (1%) Frame = +3 Query: 138 LGFDEKGQKEVKPGDSEKPVKWALEAGYRLIDTAALY-LNKIR*ERPSAPLAFRERTSSS 314 L +DEK +KEVKP D +K +K E A L ++R ER A RE + Sbjct: 2363 LSYDEKIKKEVKPLDVQKIIKQRQEEQRESQRRARLLEEQRLREEREEMERARRELEEKN 2422 Query: 315 *LSYQWTIRETW*NNWRSLWRD 380 L+ + + + N R++ D Sbjct: 2423 RLAERENLNMEYSENERAMESD 2444 >SB_3196| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 339 Score = 29.5 bits (63), Expect = 3.2 Identities = 11/24 (45%), Positives = 18/24 (75%) Frame = -3 Query: 481 FPGVQVIYNVVTFESRIKSNWIVY 410 FP Q IY+V++F+ R +SN+ +Y Sbjct: 41 FPATQGIYDVLSFQERDESNYKIY 64 >SB_57427| Best HMM Match : Exo_endo_phos (HMM E-Value=0.00044) Length = 1693 Score = 28.7 bits (61), Expect = 5.5 Identities = 11/31 (35%), Positives = 17/31 (54%) Frame = -3 Query: 157 PFSSNPRKVPRPKAGIELPSFSEIFGALPPP 65 P +S+ R++P P+ P EI +PPP Sbjct: 788 PKTSDKREIPTPEMARSFPHLREIAAHIPPP 818 >SB_55921| Best HMM Match : Aldo_ket_red (HMM E-Value=0.16) Length = 195 Score = 28.3 bits (60), Expect = 7.3 Identities = 14/36 (38%), Positives = 23/36 (63%), Gaps = 2/36 (5%) Frame = +2 Query: 314 VTKLPMDNQRDVVKQL--EKSLARLNMSYVDLYLIH 415 ++K+ +N ++ L EKSL RL + +DLYL+H Sbjct: 31 LSKVRPENASEMKMMLSCEKSLERLGIDRIDLYLLH 66 >SB_49185| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 371 Score = 28.3 bits (60), Expect = 7.3 Identities = 11/31 (35%), Positives = 18/31 (58%) Frame = +3 Query: 657 HSLLMAFFPVWPDVSTTASPFNTAPNDPTLV 749 H LL +P+ P V ASP+ ++P + L+ Sbjct: 56 HDLLNNSYPIIPKVGEPASPYVSSPRESVLI 86 >SB_16955| Best HMM Match : SLAP (HMM E-Value=0.048) Length = 1952 Score = 28.3 bits (60), Expect = 7.3 Identities = 11/31 (35%), Positives = 18/31 (58%) Frame = +3 Query: 657 HSLLMAFFPVWPDVSTTASPFNTAPNDPTLV 749 H LL +P+ P V ASP+ ++P + L+ Sbjct: 1637 HDLLNNSYPIIPKVGEPASPYVSSPRESVLI 1667 >SB_1933| Best HMM Match : PI3_PI4_kinase (HMM E-Value=4.06377e-44) Length = 915 Score = 27.9 bits (59), Expect = 9.7 Identities = 14/46 (30%), Positives = 23/46 (50%) Frame = +1 Query: 370 FGEIEHELRRSVLDTQSSCF*SWIQRLRHCRLLGHLERHGGSKKSR 507 F E E RR+V + +SC + + ++LGH + G K+ R Sbjct: 278 FNPDEKEYRRAVENFTASCAAVTVFHIDFSKILGHAQMFGNFKRDR 323 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 25,284,896 Number of Sequences: 59808 Number of extensions: 527808 Number of successful extensions: 1561 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 1361 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1555 length of database: 16,821,457 effective HSP length: 81 effective length of database: 11,977,009 effective search space used: 2119930593 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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