BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30547 (778 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g03820.1 68415.m00343 nonsense-mediated mRNA decay NMD3 famil... 128 4e-30 At2g44250.1 68415.m05506 expressed protein contains Pfam profi... 29 4.5 At3g09140.1 68416.m01075 expressed protein contains Pfam profile... 28 6.0 At5g44770.1 68418.m05487 DC1 domain-containing protein contains ... 28 7.9 At5g03360.1 68418.m00289 DC1 domain-containing protein contains ... 28 7.9 >At2g03820.1 68415.m00343 nonsense-mediated mRNA decay NMD3 family protein contains Pfam profile: PF04981 NMD3 family Length = 516 Score = 128 bits (309), Expect = 4e-30 Identities = 60/121 (49%), Positives = 82/121 (67%), Gaps = 1/121 (0%) Frame = +1 Query: 256 YLQPPSEWVVCALESREXXXXXXXXXXXXSRVKLIDAGFAWTEPHSKRIKVKLTVQGEVI 435 YLQPP W+ C ES+E ++VKL +A F WTEPHSKRIKVKLTVQ EV+ Sbjct: 65 YLQPPKTWIKCQWESKELLTFCIKRLKNLNKVKLKNAEFVWTEPHSKRIKVKLTVQAEVL 124 Query: 436 GGAVLQQTFIVEFTIQHQMCDAC-HLQKLKITGRALVQVRQRANNRKTFYYLEQLILKHK 612 GAVL+Q++ VE+T++ +C++C Q A +Q+RQ ++R+TF+YLEQLIL+H Sbjct: 125 NGAVLEQSYPVEYTVRDNLCESCSRFQANPDQWVASIQLRQHVSHRRTFFYLEQLILRHD 184 Query: 613 A 615 A Sbjct: 185 A 185 Score = 63.7 bits (148), Expect = 1e-10 Identities = 23/44 (52%), Positives = 32/44 (72%) Frame = +2 Query: 119 ILCCQCAVPIEANPSNMCVACLRAHVDITDGIPKQATLFFCRGC 250 +LCC+C VP+ N +NMCV CLR+ VDIT+G+ K +F+C C Sbjct: 19 VLCCKCGVPMAPNAANMCVNCLRSEVDITEGLQKSIQIFYCPEC 62 >At2g44250.1 68415.m05506 expressed protein contains Pfam profile PF03080: Arabidopsis proteins of unknown function Length = 409 Score = 28.7 bits (61), Expect = 4.5 Identities = 12/31 (38%), Positives = 16/31 (51%) Frame = -3 Query: 260 KYFHNPYRRIMSLVWVFHL*CLHEPLNKLHT 168 K H+P I+ VW++H PL K HT Sbjct: 51 KSIHSPDGDIIDCVWIYHQPAFDHPLLKNHT 81 >At3g09140.1 68416.m01075 expressed protein contains Pfam profile PF05056: Protein of unknown function (DUF674); expression supported by MPSS Length = 473 Score = 28.3 bits (60), Expect = 6.0 Identities = 12/19 (63%), Positives = 15/19 (78%), Gaps = 1/19 (5%) Frame = +1 Query: 151 SKPLKY-VCSLFKGSCRHH 204 ++ +KY VCS FK SCRHH Sbjct: 110 TRAVKYFVCSGFKESCRHH 128 >At5g44770.1 68418.m05487 DC1 domain-containing protein contains Pfam profile PF03107: DC1 domain Length = 541 Score = 27.9 bits (59), Expect = 7.9 Identities = 14/39 (35%), Positives = 20/39 (51%), Gaps = 1/39 (2%) Frame = +2 Query: 140 VPIEANPSNMCVACLRAHV-DITDGIPKQATLFFCRGCE 253 +P +P M ++ L H D G PK T+FFC C+ Sbjct: 6 LPFHEHP--MTISNLYNHACDFCPGRPKPGTIFFCEKCD 42 >At5g03360.1 68418.m00289 DC1 domain-containing protein contains Pfam profile PF03107: DC1 domain Length = 1610 Score = 27.9 bits (59), Expect = 7.9 Identities = 19/61 (31%), Positives = 29/61 (47%), Gaps = 2/61 (3%) Frame = +1 Query: 601 LKHKAHENTLGDQAQA*WTRFPSIQPENPCAPKWLDFNSIW--FLALIKCPTIPRKLIFR 774 L H++H + L + R S E+ C FNSI F +C T+P+KL ++ Sbjct: 1526 LVHESHMHPLFLTTKPEEWRVCSACKESDCTVTNETFNSIECDFALCFRCATLPQKLRYK 1585 Query: 775 H 777 H Sbjct: 1586 H 1586 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 16,923,478 Number of Sequences: 28952 Number of extensions: 349561 Number of successful extensions: 826 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 800 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 824 length of database: 12,070,560 effective HSP length: 80 effective length of database: 9,754,400 effective search space used: 1736283200 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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