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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS30547
         (778 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g03820.1 68415.m00343 nonsense-mediated mRNA decay NMD3 famil...   128   4e-30
At2g44250.1 68415.m05506 expressed protein   contains Pfam profi...    29   4.5  
At3g09140.1 68416.m01075 expressed protein contains Pfam profile...    28   6.0  
At5g44770.1 68418.m05487 DC1 domain-containing protein contains ...    28   7.9  
At5g03360.1 68418.m00289 DC1 domain-containing protein contains ...    28   7.9  

>At2g03820.1 68415.m00343 nonsense-mediated mRNA decay NMD3 family
           protein contains Pfam profile: PF04981 NMD3 family
          Length = 516

 Score =  128 bits (309), Expect = 4e-30
 Identities = 60/121 (49%), Positives = 82/121 (67%), Gaps = 1/121 (0%)
 Frame = +1

Query: 256 YLQPPSEWVVCALESREXXXXXXXXXXXXSRVKLIDAGFAWTEPHSKRIKVKLTVQGEVI 435
           YLQPP  W+ C  ES+E            ++VKL +A F WTEPHSKRIKVKLTVQ EV+
Sbjct: 65  YLQPPKTWIKCQWESKELLTFCIKRLKNLNKVKLKNAEFVWTEPHSKRIKVKLTVQAEVL 124

Query: 436 GGAVLQQTFIVEFTIQHQMCDAC-HLQKLKITGRALVQVRQRANNRKTFYYLEQLILKHK 612
            GAVL+Q++ VE+T++  +C++C   Q       A +Q+RQ  ++R+TF+YLEQLIL+H 
Sbjct: 125 NGAVLEQSYPVEYTVRDNLCESCSRFQANPDQWVASIQLRQHVSHRRTFFYLEQLILRHD 184

Query: 613 A 615
           A
Sbjct: 185 A 185



 Score = 63.7 bits (148), Expect = 1e-10
 Identities = 23/44 (52%), Positives = 32/44 (72%)
 Frame = +2

Query: 119 ILCCQCAVPIEANPSNMCVACLRAHVDITDGIPKQATLFFCRGC 250
           +LCC+C VP+  N +NMCV CLR+ VDIT+G+ K   +F+C  C
Sbjct: 19  VLCCKCGVPMAPNAANMCVNCLRSEVDITEGLQKSIQIFYCPEC 62


>At2g44250.1 68415.m05506 expressed protein   contains Pfam profile
           PF03080: Arabidopsis proteins of unknown function
          Length = 409

 Score = 28.7 bits (61), Expect = 4.5
 Identities = 12/31 (38%), Positives = 16/31 (51%)
 Frame = -3

Query: 260 KYFHNPYRRIMSLVWVFHL*CLHEPLNKLHT 168
           K  H+P   I+  VW++H      PL K HT
Sbjct: 51  KSIHSPDGDIIDCVWIYHQPAFDHPLLKNHT 81


>At3g09140.1 68416.m01075 expressed protein contains Pfam profile
           PF05056: Protein of unknown function (DUF674);
           expression supported by MPSS
          Length = 473

 Score = 28.3 bits (60), Expect = 6.0
 Identities = 12/19 (63%), Positives = 15/19 (78%), Gaps = 1/19 (5%)
 Frame = +1

Query: 151 SKPLKY-VCSLFKGSCRHH 204
           ++ +KY VCS FK SCRHH
Sbjct: 110 TRAVKYFVCSGFKESCRHH 128


>At5g44770.1 68418.m05487 DC1 domain-containing protein contains
           Pfam profile PF03107: DC1 domain
          Length = 541

 Score = 27.9 bits (59), Expect = 7.9
 Identities = 14/39 (35%), Positives = 20/39 (51%), Gaps = 1/39 (2%)
 Frame = +2

Query: 140 VPIEANPSNMCVACLRAHV-DITDGIPKQATLFFCRGCE 253
           +P   +P  M ++ L  H  D   G PK  T+FFC  C+
Sbjct: 6   LPFHEHP--MTISNLYNHACDFCPGRPKPGTIFFCEKCD 42


>At5g03360.1 68418.m00289 DC1 domain-containing protein contains Pfam
            profile PF03107: DC1 domain
          Length = 1610

 Score = 27.9 bits (59), Expect = 7.9
 Identities = 19/61 (31%), Positives = 29/61 (47%), Gaps = 2/61 (3%)
 Frame = +1

Query: 601  LKHKAHENTLGDQAQA*WTRFPSIQPENPCAPKWLDFNSIW--FLALIKCPTIPRKLIFR 774
            L H++H + L    +    R  S   E+ C      FNSI   F    +C T+P+KL ++
Sbjct: 1526 LVHESHMHPLFLTTKPEEWRVCSACKESDCTVTNETFNSIECDFALCFRCATLPQKLRYK 1585

Query: 775  H 777
            H
Sbjct: 1586 H 1586


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,923,478
Number of Sequences: 28952
Number of extensions: 349561
Number of successful extensions: 826
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 800
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 824
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1736283200
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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