BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30546 (784 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g17900.1 68418.m02099 expressed protein 61 9e-10 At4g08580.1 68417.m01410 microfibrillar-associated protein-relat... 61 9e-10 At2g35110.1 68415.m04307 HEM protein-related weak similarity to ... 29 2.6 At2g45180.1 68415.m05625 protease inhibitor/seed storage/lipid t... 29 4.6 At3g23780.1 68416.m02989 DNA-directed RNA polymerase family prot... 28 8.0 At1g61040.1 68414.m06872 plus-3 domain-containing protein contai... 28 8.0 >At5g17900.1 68418.m02099 expressed protein Length = 435 Score = 60.9 bits (141), Expect = 9e-10 Identities = 25/39 (64%), Positives = 33/39 (84%) Frame = +3 Query: 15 EDVFKQDFSGPTLDDHFDKTVLPKVMQVKKFGRSGRTKY 131 + +F++DFS PT +D DK++LPKVMQVK FGRSGRTK+ Sbjct: 347 DGIFQRDFSAPTGEDRLDKSILPKVMQVKHFGRSGRTKW 385 Score = 31.1 bits (67), Expect = 0.86 Identities = 17/44 (38%), Positives = 24/44 (54%), Gaps = 1/44 (2%) Frame = +1 Query: 133 THLVDQDTTEFDSAW-SNETSAARLTNFRGGMKQVFEKPSAKGS 261 THLV++DTT++ + W SN+ + GM KP KGS Sbjct: 386 THLVNEDTTDWSNPWTSNDPLREKYNKKMAGMDAPIAKP--KGS 427 >At4g08580.1 68417.m01410 microfibrillar-associated protein-related similar to Microfibrillar-associated protein 1 (Associated microfibril protein) (AMF) (Swiss-Prot:P55080) [Gallus gallus] Length = 435 Score = 60.9 bits (141), Expect = 9e-10 Identities = 25/39 (64%), Positives = 33/39 (84%) Frame = +3 Query: 15 EDVFKQDFSGPTLDDHFDKTVLPKVMQVKKFGRSGRTKY 131 + +F++DFS PT +D DK++LPKVMQVK FGRSGRTK+ Sbjct: 347 DGIFQRDFSAPTGEDRLDKSILPKVMQVKHFGRSGRTKW 385 Score = 31.5 bits (68), Expect = 0.65 Identities = 18/52 (34%), Positives = 26/52 (50%), Gaps = 1/52 (1%) Frame = +1 Query: 133 THLVDQDTTEFDSAW-SNETSAARLTNFRGGMKQVFEKPSAKGSTMFDTCQT 285 THLV++DTT++ + W SN+ + GM KP KGS +T Sbjct: 386 THLVNEDTTDWSNPWTSNDPLREKYNKKMAGMDAPIAKP--KGSKKMKDWET 435 >At2g35110.1 68415.m04307 HEM protein-related weak similarity to Membrane-associated protein Hem (Dhem-2) (Swiss-Prot:P55162) [Drosophila melanogaster]; weak similarity to Nck-associated protein 1 (NAP 1) (p125Nap1) (Membrane-associated protein HEM-2) (Swiss-Prot:P55161) [Rattus norvegicus] Length = 1339 Score = 29.5 bits (63), Expect = 2.6 Identities = 12/26 (46%), Positives = 18/26 (69%) Frame = +3 Query: 51 LDDHFDKTVLPKVMQVKKFGRSGRTK 128 L + + VLP+V++ KK +SGRTK Sbjct: 339 LHEDYQLYVLPRVLESKKMAKSGRTK 364 >At2g45180.1 68415.m05625 protease inhibitor/seed storage/lipid transfer protein (LTP) family protein similar to 14 kDa polypeptide [Catharanthus roseus] GI:407410; contains Pfam protease inhibitor/seed storage/LTP family domain PF00234 Length = 134 Score = 28.7 bits (61), Expect = 4.6 Identities = 14/29 (48%), Positives = 15/29 (51%) Frame = +1 Query: 694 ICLRLFDSVVVTSTKCPCIQDKPGNVPKN 780 I L L VTSTKCP KP PK+ Sbjct: 13 ITLNLLFFTFVTSTKCPPTTPKPPKTPKS 41 >At3g23780.1 68416.m02989 DNA-directed RNA polymerase family protein similar to SP|P38420 DNA-directed RNA polymerase II 135 kDa polypeptide (EC 2.7.7.6) (RNA polymerase II subunit 2) {Arabidopsis thaliana}; contains Pfam profiles PF04560: RNA polymerase Rpb2 domain 7, PF04561: RNA polymerase Rpb2 domain 2, PF04565: RNA polymerase Rpb2 domain 3, PF04566: RNA polymerase Rpb2 domain 4, PF04567: RNA polymerase Rpb2 domain 5 Length = 946 Score = 27.9 bits (59), Expect = 8.0 Identities = 10/29 (34%), Positives = 19/29 (65%) Frame = +1 Query: 4 WIKKKMFSNRTSRVLRWMIILTRQFYQRL 90 W +++++ R+ ++R MI + FYQRL Sbjct: 786 WGNERVYNGRSGEMMRSMIFMGPTFYQRL 814 >At1g61040.1 68414.m06872 plus-3 domain-containing protein contains Pfam profile PF03126: Plus-3 domain Length = 643 Score = 27.9 bits (59), Expect = 8.0 Identities = 15/40 (37%), Positives = 23/40 (57%), Gaps = 1/40 (2%) Frame = +1 Query: 85 RLCKSRSLDVPVVRSTTHLVDQDTTEF-DSAWSNETSAAR 201 RLC +++D T L ++ T ++ + W NETSAAR Sbjct: 309 RLCWVKNVDATDPDKTYKLENKTTHKYLNVVWGNETSAAR 348 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 17,312,288 Number of Sequences: 28952 Number of extensions: 357980 Number of successful extensions: 729 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 717 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 729 length of database: 12,070,560 effective HSP length: 80 effective length of database: 9,754,400 effective search space used: 1755792000 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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