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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS30546
         (784 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g17900.1 68418.m02099 expressed protein                             61   9e-10
At4g08580.1 68417.m01410 microfibrillar-associated protein-relat...    61   9e-10
At2g35110.1 68415.m04307 HEM protein-related weak similarity to ...    29   2.6  
At2g45180.1 68415.m05625 protease inhibitor/seed storage/lipid t...    29   4.6  
At3g23780.1 68416.m02989 DNA-directed RNA polymerase family prot...    28   8.0  
At1g61040.1 68414.m06872 plus-3 domain-containing protein contai...    28   8.0  

>At5g17900.1 68418.m02099 expressed protein
          Length = 435

 Score = 60.9 bits (141), Expect = 9e-10
 Identities = 25/39 (64%), Positives = 33/39 (84%)
 Frame = +3

Query: 15  EDVFKQDFSGPTLDDHFDKTVLPKVMQVKKFGRSGRTKY 131
           + +F++DFS PT +D  DK++LPKVMQVK FGRSGRTK+
Sbjct: 347 DGIFQRDFSAPTGEDRLDKSILPKVMQVKHFGRSGRTKW 385



 Score = 31.1 bits (67), Expect = 0.86
 Identities = 17/44 (38%), Positives = 24/44 (54%), Gaps = 1/44 (2%)
 Frame = +1

Query: 133 THLVDQDTTEFDSAW-SNETSAARLTNFRGGMKQVFEKPSAKGS 261
           THLV++DTT++ + W SN+    +      GM     KP  KGS
Sbjct: 386 THLVNEDTTDWSNPWTSNDPLREKYNKKMAGMDAPIAKP--KGS 427


>At4g08580.1 68417.m01410 microfibrillar-associated protein-related
           similar to Microfibrillar-associated protein 1
           (Associated microfibril protein) (AMF)
           (Swiss-Prot:P55080) [Gallus gallus]
          Length = 435

 Score = 60.9 bits (141), Expect = 9e-10
 Identities = 25/39 (64%), Positives = 33/39 (84%)
 Frame = +3

Query: 15  EDVFKQDFSGPTLDDHFDKTVLPKVMQVKKFGRSGRTKY 131
           + +F++DFS PT +D  DK++LPKVMQVK FGRSGRTK+
Sbjct: 347 DGIFQRDFSAPTGEDRLDKSILPKVMQVKHFGRSGRTKW 385



 Score = 31.5 bits (68), Expect = 0.65
 Identities = 18/52 (34%), Positives = 26/52 (50%), Gaps = 1/52 (1%)
 Frame = +1

Query: 133 THLVDQDTTEFDSAW-SNETSAARLTNFRGGMKQVFEKPSAKGSTMFDTCQT 285
           THLV++DTT++ + W SN+    +      GM     KP  KGS      +T
Sbjct: 386 THLVNEDTTDWSNPWTSNDPLREKYNKKMAGMDAPIAKP--KGSKKMKDWET 435


>At2g35110.1 68415.m04307 HEM protein-related weak similarity to
           Membrane-associated protein Hem (Dhem-2)
           (Swiss-Prot:P55162) [Drosophila melanogaster]; weak
           similarity to Nck-associated protein 1 (NAP 1)
           (p125Nap1) (Membrane-associated protein HEM-2)
           (Swiss-Prot:P55161) [Rattus norvegicus]
          Length = 1339

 Score = 29.5 bits (63), Expect = 2.6
 Identities = 12/26 (46%), Positives = 18/26 (69%)
 Frame = +3

Query: 51  LDDHFDKTVLPKVMQVKKFGRSGRTK 128
           L + +   VLP+V++ KK  +SGRTK
Sbjct: 339 LHEDYQLYVLPRVLESKKMAKSGRTK 364


>At2g45180.1 68415.m05625 protease inhibitor/seed storage/lipid
           transfer protein (LTP) family protein similar to 14 kDa
           polypeptide [Catharanthus roseus] GI:407410; contains
           Pfam protease inhibitor/seed storage/LTP family domain
           PF00234
          Length = 134

 Score = 28.7 bits (61), Expect = 4.6
 Identities = 14/29 (48%), Positives = 15/29 (51%)
 Frame = +1

Query: 694 ICLRLFDSVVVTSTKCPCIQDKPGNVPKN 780
           I L L     VTSTKCP    KP   PK+
Sbjct: 13  ITLNLLFFTFVTSTKCPPTTPKPPKTPKS 41


>At3g23780.1 68416.m02989 DNA-directed RNA polymerase family protein
           similar to SP|P38420 DNA-directed RNA polymerase II 135
           kDa polypeptide (EC 2.7.7.6) (RNA polymerase II subunit
           2) {Arabidopsis thaliana}; contains Pfam profiles
           PF04560: RNA polymerase Rpb2 domain 7, PF04561: RNA
           polymerase Rpb2 domain 2, PF04565: RNA polymerase Rpb2
           domain 3, PF04566: RNA polymerase Rpb2 domain 4,
           PF04567: RNA polymerase Rpb2 domain 5
          Length = 946

 Score = 27.9 bits (59), Expect = 8.0
 Identities = 10/29 (34%), Positives = 19/29 (65%)
 Frame = +1

Query: 4   WIKKKMFSNRTSRVLRWMIILTRQFYQRL 90
           W  +++++ R+  ++R MI +   FYQRL
Sbjct: 786 WGNERVYNGRSGEMMRSMIFMGPTFYQRL 814


>At1g61040.1 68414.m06872 plus-3 domain-containing protein contains
           Pfam profile PF03126: Plus-3 domain
          Length = 643

 Score = 27.9 bits (59), Expect = 8.0
 Identities = 15/40 (37%), Positives = 23/40 (57%), Gaps = 1/40 (2%)
 Frame = +1

Query: 85  RLCKSRSLDVPVVRSTTHLVDQDTTEF-DSAWSNETSAAR 201
           RLC  +++D      T  L ++ T ++ +  W NETSAAR
Sbjct: 309 RLCWVKNVDATDPDKTYKLENKTTHKYLNVVWGNETSAAR 348


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,312,288
Number of Sequences: 28952
Number of extensions: 357980
Number of successful extensions: 729
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 717
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 729
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1755792000
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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