BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30530 (798 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 165 9e-42 SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 160 2e-40 SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 86 5e-18 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 53 4e-08 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 31 0.19 SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1||... 29 0.77 SPCC16C4.07 |scw1||RNA-binding protein Scw1|Schizosaccharomyces ... 27 3.1 SPAC227.01c ||SPAPB21F2.04c|Erd1 homolog|Schizosaccharomyces pom... 27 4.1 SPBC428.10 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 5.4 SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1||... 26 5.4 SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 26 5.4 SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch... 26 7.2 SPAC890.02c |alp7|mia1|TACC homolog |Schizosaccharomyces pombe|c... 26 7.2 SPBC4C3.05c |nuc1|rpa1|DNA-directed RNA polymerase I complex lar... 25 9.5 SPAC25H1.06 |||histone acetyltransferase complex subunit |Schizo... 25 9.5 SPBC725.11c |php2||CCAAT-binding factor complex subunit Php2 |Sc... 25 9.5 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 165 bits (400), Expect = 9e-42 Identities = 82/144 (56%), Positives = 101/144 (70%), Gaps = 1/144 (0%) Frame = +3 Query: 255 RFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFEPANQM 434 RF G+LNVDL EFQTNLVPYPRIHFPLVTY+P++SA KA+HE SV EITN CFEP NQM Sbjct: 247 RFAGSLNVDLNEFQTNLVPYPRIHFPLVTYSPIVSAAKAFHESNSVQEITNQCFEPYNQM 306 Query: 435 VKCDPRHGKYMACCMLYRGDVVPKE*TRPSLPSKPSVLSNSSTGVQPV-FKVGINYQPTN 611 VKCDPR G+YMA C+LYRGDV+P++ + ++ S S + P FK+GI Y+P Sbjct: 307 VKCDPRTGRYMATCLLYRGDVIPRD-VQAAVTSIKSRRTIQFVDWCPTGFKIGICYEPPQ 365 Query: 612 VVPGGDLGQRFKRCRSCMFVQTTT 683 VPG + + R CM TT+ Sbjct: 366 HVPGSGIA-KVNRA-VCMLSNTTS 387 Score = 154 bits (373), Expect = 2e-38 Identities = 68/85 (80%), Positives = 79/85 (92%) Frame = +1 Query: 1 SVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNL 180 +++YGKKS L+F++YPAPQVST+VVEPYNS+LTTH TL++SDC FMVDNEA YDICRRNL Sbjct: 162 NMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNL 221 Query: 181 DIERPTYTNLNRLIGQIVSSITASL 255 DIERPTY NLNRLI Q+VSSITASL Sbjct: 222 DIERPTYENLNRLIAQVVSSITASL 246 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 160 bits (388), Expect = 2e-40 Identities = 77/143 (53%), Positives = 95/143 (66%) Frame = +3 Query: 255 RFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFEPANQM 434 RF+G+LNVDL EFQTNLVPYPRIHFPLVTYAP++SA KA+HE SV EITN CFEP NQM Sbjct: 243 RFEGSLNVDLAEFQTNLVPYPRIHFPLVTYAPIVSAAKAFHESNSVQEITNQCFEPYNQM 302 Query: 435 VKCDPRHGKYMACCMLYRGDVVPKE*TRPSLPSKPSVLSNSSTGVQPVFKVGINYQPTNV 614 VKCDPR G+YMA C+LYRGDV+P++ K FK+GI +P Sbjct: 303 VKCDPRAGRYMATCLLYRGDVIPRDVQAAVTTIKAKRTIQFVDWCPTGFKIGICDRPPQH 362 Query: 615 VPGGDLGQRFKRCRSCMFVQTTT 683 + G ++ + + CM TT+ Sbjct: 363 IEGSEIAKVDRAV--CMLSNTTS 383 Score = 147 bits (356), Expect = 2e-36 Identities = 65/85 (76%), Positives = 78/85 (91%) Frame = +1 Query: 1 SVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNL 180 +++Y KKSKL+F++YPAPQVST+VVEPYNS+LTTH TL+ +DC FMVDNE+ YDICRRNL Sbjct: 158 AMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNL 217 Query: 181 DIERPTYTNLNRLIGQIVSSITASL 255 DIERP+Y NLNRLI Q+VSSITASL Sbjct: 218 DIERPSYENLNRLIAQVVSSITASL 242 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 86.2 bits (204), Expect = 5e-18 Identities = 38/86 (44%), Positives = 54/86 (62%) Frame = +3 Query: 252 FRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFEPANQ 431 FRF G LN DL + N+VP+PR+HF +V +AP+ + + + +SV E+T F+ N Sbjct: 240 FRFPGELNSDLRKLAVNMVPFPRLHFFMVGFAPLAAIGSSSFQAVSVPELTQQMFDANNM 299 Query: 432 MVKCDPRHGKYMACCMLYRGDVVPKE 509 MV DPRHG+Y+ L+RG V KE Sbjct: 300 MVAADPRHGRYLTVAALFRGKVSMKE 325 Score = 73.7 bits (173), Expect = 3e-14 Identities = 32/82 (39%), Positives = 51/82 (62%) Frame = +1 Query: 7 DYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDI 186 +Y + F++ PAP+ S VVEPYN+ L+ H +E+SD F +DNEA+ I L I Sbjct: 158 EYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKI 217 Query: 187 ERPTYTNLNRLIGQIVSSITAS 252 + P+Y +LN L+ +++ +T S Sbjct: 218 KSPSYDDLNHLVSAVMAGVTTS 239 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 53.2 bits (122), Expect = 4e-08 Identities = 27/83 (32%), Positives = 46/83 (55%), Gaps = 1/83 (1%) Frame = +1 Query: 10 YGKKSKLEFAIYPAPQ-VSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDI 186 Y KK ++++P Q VS VV+PYNS+L ++D ++DN A+ I L Sbjct: 161 YPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHT 220 Query: 187 ERPTYTNLNRLIGQIVSSITASL 255 + PT+ N+L+ ++S+ T +L Sbjct: 221 QNPTFHQQNQLVSTVMSASTTTL 243 Score = 45.2 bits (102), Expect = 1e-05 Identities = 28/110 (25%), Positives = 47/110 (42%), Gaps = 4/110 (3%) Frame = +3 Query: 192 PNLHQPESSHRTDCLLDHCFFRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVIS---A 362 P HQ T R+ G +N DL +L+P PR HF L +Y P + Sbjct: 223 PTFHQQNQLVSTVMSASTTTLRYPGYMNNDLVSIIASLIPSPRCHFLLTSYTPFTNQQVE 282 Query: 363 EKAYHEQLSVAEITNACFEPANQMVKCDP-RHGKYMACCMLYRGDVVPKE 509 E + +V ++ P NQMV +P + +++ + +G+ P + Sbjct: 283 EAKAIRKTTVLDVMRRLLLPKNQMVSVNPSKKSCFISILDIIQGEADPAD 332 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 31.1 bits (67), Expect = 0.19 Identities = 27/89 (30%), Positives = 41/89 (46%), Gaps = 2/89 (2%) Frame = +1 Query: 286 PSSRLTWCLTPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSPPTRW*NATPVMAST 465 P+++ P S + T +SS+P P N+ PSP S + AS+ P R T V S Sbjct: 1232 PTAKAPPVPAPSSEAPSVSTPRSSVPS-PHSNASPSPTSSSMASAAPAR----TSVSRSK 1286 Query: 466 WLAVCCTVVTSYPRSERG--HRYHQNQAY 546 A TS +S + H +H N+ + Sbjct: 1287 SKAERHETSTSSRKSSKSGEHHHHHNEGH 1315 >SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1|||Manual Length = 3227 Score = 29.1 bits (62), Expect = 0.77 Identities = 20/68 (29%), Positives = 33/68 (48%) Frame = -2 Query: 512 SLLGYDVTTVQHTASHVLAMTGVAFHHLVGGLEACVCDLGDGKLFMVGLLGRDDWRVRDQ 333 SLLGY ++ A++V+A + V HL+ G D + K G D R Q Sbjct: 412 SLLGYHLSVKTPQATYVVARSIVMLDHLIDGYSMAFPDFSESK-------GLDMLVDRVQ 464 Query: 332 WEVDTGVR 309 +E++ G++ Sbjct: 465 YELEAGLQ 472 >SPCC16C4.07 |scw1||RNA-binding protein Scw1|Schizosaccharomyces pombe|chr 3|||Manual Length = 561 Score = 27.1 bits (57), Expect = 3.1 Identities = 23/74 (31%), Positives = 34/74 (45%), Gaps = 2/74 (2%) Frame = +1 Query: 415 SSPPTRW*NATPVMASTWLAVCCTVVTSYPRSERGHRYH-QN-QAYYPIRRLVSNRFSRS 588 SS R P++ L++ V +Y SE+G+ H QN A P R+ S + + Sbjct: 308 SSTNLRQDGVPPILRFNSLSINTNVARNYLSSEKGYSAHTQNSSAQSPHPRVFS--ANSA 365 Query: 589 VSTTSQPTWCPEAT 630 STTS P P + Sbjct: 366 FSTTSPPPLTPSTS 379 >SPAC227.01c ||SPAPB21F2.04c|Erd1 homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 373 Score = 26.6 bits (56), Expect = 4.1 Identities = 14/30 (46%), Positives = 16/30 (53%) Frame = +1 Query: 709 SAFDQQSFGTFIVTAKAVXFRGNWVTFRVE 798 SAF FG FI+ V R WV FR+E Sbjct: 320 SAFFTTDFGIFIMQFLEVFRRCVWVFFRIE 349 >SPBC428.10 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 751 Score = 26.2 bits (55), Expect = 5.4 Identities = 21/63 (33%), Positives = 33/63 (52%) Frame = +1 Query: 235 SSITASLDSTAL*MWTSPSSRLTWCLTPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHA 414 SS ASL+ + SPS LT L S++ S + + + R+ T +S SP+ Q+ Sbjct: 605 SSPNASLEQCFV--QRSPSKMLT-TLRNNSSTFPSLRKNAMIARKSTADSLSSPKRQSVP 661 Query: 415 SSP 423 S+P Sbjct: 662 STP 664 >SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1158 Score = 26.2 bits (55), Expect = 5.4 Identities = 11/28 (39%), Positives = 17/28 (60%) Frame = +1 Query: 349 QSSLPRRPTMNSFPSPRSQTHASSPPTR 432 + SLPRRP+ +P S T ++ PP + Sbjct: 738 RGSLPRRPSSALLTNPISITKSNPPPVK 765 >SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 800 Score = 26.2 bits (55), Expect = 5.4 Identities = 20/77 (25%), Positives = 36/77 (46%), Gaps = 2/77 (2%) Frame = +1 Query: 280 TSPSSRLTWCLTPVSTSHWSRTRQSSLPRRPTM--NSFPSPRSQTHASSPPTRW*NATPV 453 TS S L+ + +++ T S+ P PT+ S +P + T+ ++ + +TPV Sbjct: 381 TSSSPLLSTSTSCTTSTSIPPTGNSTTPVTPTVPPTSSSTPLTTTNCTTSTSVPYTSTPV 440 Query: 454 MASTWLAVCCTVVTSYP 504 ++ CT TS P Sbjct: 441 TSTPLATTNCTTSTSVP 457 >SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosaccharomyces pombe|chr 1|||Manual Length = 157 Score = 25.8 bits (54), Expect = 7.2 Identities = 10/20 (50%), Positives = 11/20 (55%) Frame = -1 Query: 504 WVRRHHGTAYSKPCTCHDGG 445 W R H Y+KPC DGG Sbjct: 16 WRRDHPFGFYAKPCKSSDGG 35 >SPAC890.02c |alp7|mia1|TACC homolog |Schizosaccharomyces pombe|chr 1|||Manual Length = 474 Score = 25.8 bits (54), Expect = 7.2 Identities = 16/51 (31%), Positives = 31/51 (60%) Frame = +1 Query: 310 LTPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSPPTRW*NATPVMAS 462 LTP + S +S + +S +R ++N+ PS + +H+SS T + PV+++ Sbjct: 130 LTPKNPSLFSSSNAAS--QRGSLNTAPSNFAYSHSSSLQTSASSRPPVLSN 178 >SPBC4C3.05c |nuc1|rpa1|DNA-directed RNA polymerase I complex large subunit Nuc1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1689 Score = 25.4 bits (53), Expect = 9.5 Identities = 20/49 (40%), Positives = 26/49 (53%), Gaps = 4/49 (8%) Frame = +1 Query: 634 AKGSNVAV--LACLSKQQRAIGRK--LWGSAFDQQSFGTFIVTAKAVXF 768 AKGSNV V ++CL QQ GR+ L S SF + +AK+ F Sbjct: 946 AKGSNVNVSQISCLLGQQELEGRRVPLMVSGKSLPSFVPYETSAKSGGF 994 >SPAC25H1.06 |||histone acetyltransferase complex subunit |Schizosaccharomyces pombe|chr 1|||Manual Length = 408 Score = 25.4 bits (53), Expect = 9.5 Identities = 15/44 (34%), Positives = 22/44 (50%) Frame = +1 Query: 241 ITASLDSTAL*MWTSPSSRLTWCLTPVSTSHWSRTRQSSLPRRP 372 +TA+ DS L + SR T CLTPV H+ + + + P Sbjct: 199 VTAANDSKILEWDLNNFSRDTRCLTPVKDFHYDDSPVNDVEYHP 242 >SPBC725.11c |php2||CCAAT-binding factor complex subunit Php2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 334 Score = 25.4 bits (53), Expect = 9.5 Identities = 11/34 (32%), Positives = 17/34 (50%) Frame = +3 Query: 531 SKPSVLSNSSTGVQPVFKVGINYQPTNVVPGGDL 632 S+PS +SNSS P+ + P N G ++ Sbjct: 123 SRPSSISNSSESPAPINSATASMSPANNTSGNNI 156 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,526,738 Number of Sequences: 5004 Number of extensions: 79550 Number of successful extensions: 266 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 242 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 262 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 389395636 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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