BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30526 (800 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizo... 152 6e-38 SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizo... 152 6e-38 SPBP23A10.13 |orc4|orp4|origin recognition complex subunit Orc4|... 31 0.25 SPBC4.03c |||COPII-coated vesicle component Sfb3 |Schizosaccharo... 27 3.1 SPAC23D3.12 |||inorganic phosphate transporter |Schizosaccharomy... 26 5.4 SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large subunit|... 26 5.4 SPBC1271.09 |||glycerophosphodiester transporter|Schizosaccharom... 25 9.5 SPCC1672.02c |sap1||switch-activating protein Sap1|Schizosacchar... 25 9.5 SPBP4H10.09 |rsv1||transcription factor Rsv1 |Schizosaccharomyce... 25 9.5 SPBC1683.01 |||inorganic phosphate transporter |Schizosaccharomy... 25 9.5 >SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizosaccharomyces pombe|chr 2|||Manual Length = 363 Score = 152 bits (368), Expect = 6e-38 Identities = 67/81 (82%), Positives = 73/81 (90%) Frame = +3 Query: 15 LPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKEAGHQTSAESWGTGRAVARIPRVRGGG 194 LPFVFKAPIRPDLV VH +++KN RQPY VS++AGHQTSAESWGTGRA+ARIPRV GGG Sbjct: 24 LPFVFKAPIRPDLVRSVHTAVAKNKRQPYAVSEKAGHQTSAESWGTGRALARIPRVGGGG 83 Query: 195 THRSGQGAFGNMCRGGRMFAP 257 THRSGQ AFGNMCR GRMFAP Sbjct: 84 THRSGQAAFGNMCRSGRMFAP 104 Score = 81.8 bits (193), Expect = 1e-16 Identities = 47/129 (36%), Positives = 66/129 (51%) Frame = +2 Query: 254 PTKPWRRWHXXXXXXXXXXXXXXXXXXXXXXXXXQARGHIIEKIPELPLVVADKVQEINK 433 PTK WR+WH ARGH IE+IPE+PLVV D VQ K Sbjct: 104 PTKTWRKWHVKVNQNEKRYAIASAVAASGVPSLLLARGHRIEEIPEVPLVVDDAVQSFQK 163 Query: 434 TKQAVIFLRRLKAWSDILKVYKSQRFVLVRVKCATVVVSSVRGPLHNLQQGSGV*LAAFP 613 TK+AV L+ +KA+ D++KV S++ + K RGPL + +G+ + AF Sbjct: 164 TKEAVALLKEIKAYRDVIKVANSRKLRAGKGKLRNRRHVQRRGPLVVFNEDTGI-VKAF- 221 Query: 614 QTFPGVELL 640 + PGVE++ Sbjct: 222 RNIPGVEIV 230 >SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizosaccharomyces pombe|chr 2|||Manual Length = 363 Score = 152 bits (368), Expect = 6e-38 Identities = 67/81 (82%), Positives = 73/81 (90%) Frame = +3 Query: 15 LPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKEAGHQTSAESWGTGRAVARIPRVRGGG 194 LPFVFKAPIRPDLV VH +++KN RQPY VS++AGHQTSAESWGTGRA+ARIPRV GGG Sbjct: 24 LPFVFKAPIRPDLVRSVHTAVAKNKRQPYAVSEKAGHQTSAESWGTGRALARIPRVGGGG 83 Query: 195 THRSGQGAFGNMCRGGRMFAP 257 THRSGQ AFGNMCR GRMFAP Sbjct: 84 THRSGQAAFGNMCRSGRMFAP 104 Score = 81.4 bits (192), Expect = 1e-16 Identities = 47/129 (36%), Positives = 66/129 (51%) Frame = +2 Query: 254 PTKPWRRWHXXXXXXXXXXXXXXXXXXXXXXXXXQARGHIIEKIPELPLVVADKVQEINK 433 PTK WR+WH ARGH IE+IPE+PLVV D VQ K Sbjct: 104 PTKTWRKWHVKVNQNEKRYAISSAVAASGVPSLLLARGHRIEEIPEVPLVVDDAVQSFQK 163 Query: 434 TKQAVIFLRRLKAWSDILKVYKSQRFVLVRVKCATVVVSSVRGPLHNLQQGSGV*LAAFP 613 TK+AV L+ +KA+ D++KV S++ + K RGPL + +G+ + AF Sbjct: 164 TKEAVALLKEIKAYRDVVKVANSRKLRAGKGKLRNRRHVQRRGPLVVFNEDAGI-VKAF- 221 Query: 614 QTFPGVELL 640 + PGVE++ Sbjct: 222 RNIPGVEIV 230 >SPBP23A10.13 |orc4|orp4|origin recognition complex subunit Orc4|Schizosaccharomyces pombe|chr 2|||Manual Length = 972 Score = 30.7 bits (66), Expect = 0.25 Identities = 12/35 (34%), Positives = 19/35 (54%) Frame = -1 Query: 788 VFLPNEPRKRGSKTLARKCGTWNSKIDENVPKIGL 684 + +P P S L+R+C W ++D NV +GL Sbjct: 920 LIIPVHPSTNVSGALSRQCQLWQPEVDINVVSVGL 954 >SPBC4.03c |||COPII-coated vesicle component Sfb3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 891 Score = 27.1 bits (57), Expect = 3.1 Identities = 19/65 (29%), Positives = 29/65 (44%), Gaps = 3/65 (4%) Frame = -2 Query: 418 DFVGYNQGKLGNLFNNVSS-SLNERWDAGSSNGCRQGR--SPLSEVDATVPTPPGLRGAN 248 DFVGY QG + F +S ++ D +++G G P +E+ P + N Sbjct: 151 DFVGYEQGNSSSKFVQFTSYAIPATNDVCNASGIPLGMIVQPFAELRPDEAPVPVVDNTN 210 Query: 247 IRPPR 233 PPR Sbjct: 211 SNPPR 215 >SPAC23D3.12 |||inorganic phosphate transporter |Schizosaccharomyces pombe|chr 1|||Manual Length = 559 Score = 26.2 bits (55), Expect = 5.4 Identities = 10/18 (55%), Positives = 13/18 (72%) Frame = +1 Query: 583 RIRGLTRGLSANIPRCGA 636 R+RG GLSA + +CGA Sbjct: 468 RVRGTAHGLSAALGKCGA 485 >SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large subunit|Schizosaccharomyces pombe|chr 2|||Manual Length = 1752 Score = 26.2 bits (55), Expect = 5.4 Identities = 16/53 (30%), Positives = 22/53 (41%) Frame = +3 Query: 147 GTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPRSPGGVGTVASTSDSG 305 G+ A A P V+GG R G G +G + +SPG +S G Sbjct: 1498 GSPDAAAFSPLVQGGSEGREGFGDYGLLGAASPYKGVQSPGYTSPFSSAMSPG 1550 >SPBC1271.09 |||glycerophosphodiester transporter|Schizosaccharomyces pombe|chr 2|||Manual Length = 543 Score = 25.4 bits (53), Expect = 9.5 Identities = 11/21 (52%), Positives = 15/21 (71%) Frame = +1 Query: 574 SSTRIRGLTRGLSANIPRCGA 636 S T IRG+ G++A I +CGA Sbjct: 426 SPTAIRGVYYGIAAAIGKCGA 446 >SPCC1672.02c |sap1||switch-activating protein Sap1|Schizosaccharomyces pombe|chr 3|||Manual Length = 254 Score = 25.4 bits (53), Expect = 9.5 Identities = 13/34 (38%), Positives = 21/34 (61%), Gaps = 1/34 (2%) Frame = +3 Query: 645 VNKLEPP*SWAPGKANL-WNVFVNLGIPGSAFSG 743 ++KLEPP ++AP N+ N+ N+G +A G Sbjct: 208 LSKLEPPSAYAPHGVNMGTNMGANMGANMNAIRG 241 >SPBP4H10.09 |rsv1||transcription factor Rsv1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 428 Score = 25.4 bits (53), Expect = 9.5 Identities = 14/49 (28%), Positives = 19/49 (38%) Frame = -1 Query: 779 PNEPRKRGSKTLARKCGTWNSKIDENVPKIGLPRSPASGRFKLVNIPGA 633 P G +TL W+++ + GLP S F V IP A Sbjct: 224 PRFQNTNGQRTLTVPVSVWDARQPPTSSRSGLPLSVMYNHFPSVPIPPA 272 >SPBC1683.01 |||inorganic phosphate transporter |Schizosaccharomyces pombe|chr 2|||Manual Length = 573 Score = 25.4 bits (53), Expect = 9.5 Identities = 9/18 (50%), Positives = 13/18 (72%) Frame = +1 Query: 583 RIRGLTRGLSANIPRCGA 636 R+RG G+SA + +CGA Sbjct: 480 RVRGTAHGISAALGKCGA 497 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,464,690 Number of Sequences: 5004 Number of extensions: 75087 Number of successful extensions: 240 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 216 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 235 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 388424860 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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