Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30520
(617 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC11C11.09c |rpl502|rpl5-2, rpl5b|60S ribosomal protein L5|Sch... 83 3e-17
SPAC3H5.12c |rpl501|rpl5-1, rpl5|60S ribosomal protein L5|Schizo... 83 3e-17
SPAC19A8.08 |upf2||nonsense-mediated decay protein Upf2|Schizosa... 27 1.6
SPBC17D11.05 |tif32||translation initiation factor eIF3a|Schizos... 27 1.6
SPCC162.08c |nup211||nuclear pore complex associated protein|Sch... 27 1.6
SPCC63.14 |||conserved fungal protein|Schizosaccharomyces pombe|... 27 2.2
SPBC646.10c |||U3 snoRNP protein Nop56 |Schizosaccharomyces pomb... 27 2.2
SPCC330.19c |||sequence orphan|Schizosaccharomyces pombe|chr 3||... 27 2.9
SPBC1347.01c |rev1|SPBC215.16c|deoxycytidyl transferase Rev1 |Sc... 27 2.9
SPBC11G11.05 |rpa34||DNA-directed RNA polymerase I complex subun... 27 2.9
SPAC29A4.04c |||pseudouridylate synthase |Schizosaccharomyces po... 26 3.8
SPAC890.05 |||ribosome biogenesis protein|Schizosaccharomyces po... 26 3.8
SPAC23C4.17 |||tRNA |Schizosaccharomyces pombe|chr 1|||Manual 26 3.8
SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyc... 26 5.0
SPBC1709.02c |vas2|SPBC1734.18c|valine-tRNA ligase Vas2 |Schizos... 26 5.0
SPBP8B7.09c |||karyopherin|Schizosaccharomyces pombe|chr 2|||Manual 25 6.6
SPAC24B11.12c |||P-type ATPase |Schizosaccharomyces pombe|chr 1|... 25 8.8
SPBC4F6.07c |||ATP-dependent RNA helicase Mak5 |Schizosaccharomy... 25 8.8
>SPBC11C11.09c |rpl502|rpl5-2, rpl5b|60S ribosomal protein
L5|Schizosaccharomyces pombe|chr 2|||Manual
Length = 294
Score = 83.0 bits (196), Expect = 3e-17
Identities = 35/56 (62%), Positives = 46/56 (82%)
Frame = +1
Query: 85 EPVDNGPGAFRCYLDVGLARTTTGARVFGAMKGAVDGGLNVPHSIKRFPGYDAESK 252
E +++GP F+ +LDVGL RT+TG+RVFGAMKGA DGGL +PHS RFPG+D E++
Sbjct: 133 EAIEDGPRPFKVFLDVGLKRTSTGSRVFGAMKGASDGGLFIPHSPNRFPGFDIETE 188
Score = 74.5 bits (175), Expect = 1e-14
Identities = 41/109 (37%), Positives = 60/109 (55%), Gaps = 2/109 (1%)
Frame = +3
Query: 222 KIPWL*CRIQKFNAEVHRAHIFGLHVAEYMRSLEQDDEDSFKRQFSKYIKLGVTADAIEA 401
+ P ++ + E R +I+G HVAEYM L DDE+ +++QFS I G+ +D +E
Sbjct: 179 RFPGFDIETEELDDETLRKYIYGGHVAEYMEMLIDDDEERYQKQFSGLIADGIESDQLED 238
Query: 402 IYKKAHEAIRADPSHKKKELKKDSVKQKRWEQTQAKLA--ERKNRIKQK 542
IY +A+ IR DPS +K + K + + TQ KL ERK R K
Sbjct: 239 IYAEAYAKIREDPSFQKSGKDAAAFKAESLKYTQRKLTAEERKERFNAK 287
>SPAC3H5.12c |rpl501|rpl5-1, rpl5|60S ribosomal protein
L5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 294
Score = 83.0 bits (196), Expect = 3e-17
Identities = 35/56 (62%), Positives = 46/56 (82%)
Frame = +1
Query: 85 EPVDNGPGAFRCYLDVGLARTTTGARVFGAMKGAVDGGLNVPHSIKRFPGYDAESK 252
E +++GP F+ +LDVGL RT+TG+RVFGAMKGA DGGL +PHS RFPG+D E++
Sbjct: 133 EAIEDGPRPFKVFLDVGLKRTSTGSRVFGAMKGASDGGLFIPHSPNRFPGFDIETE 188
Score = 74.9 bits (176), Expect = 8e-15
Identities = 41/109 (37%), Positives = 60/109 (55%), Gaps = 2/109 (1%)
Frame = +3
Query: 222 KIPWL*CRIQKFNAEVHRAHIFGLHVAEYMRSLEQDDEDSFKRQFSKYIKLGVTADAIEA 401
+ P ++ + E R +I+G HVAEYM L DDE+ +++QFS I G+ +D +E
Sbjct: 179 RFPGFDIETEELDDETLRKYIYGGHVAEYMEMLIDDDEERYQKQFSGLIADGIESDQLED 238
Query: 402 IYKKAHEAIRADPSHKKKELKKDSVKQKRWEQTQAKLA--ERKNRIKQK 542
IY +A+ IR DPS +K + K + + TQ KL ERK R K
Sbjct: 239 IYAEAYAKIREDPSFQKSGKDAAAFKAESLKHTQRKLTAEERKERFNAK 287
>SPAC19A8.08 |upf2||nonsense-mediated decay protein
Upf2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1049
Score = 27.5 bits (58), Expect = 1.6
Identities = 23/101 (22%), Positives = 40/101 (39%)
Frame = +3
Query: 255 FNAEVHRAHIFGLHVAEYMRSLEQDDEDSFKRQFSKYIKLGVTADAIEAIYKKAHEAIRA 434
FNA R+ + A+ D E F + G A+ +IY+ + E IR+
Sbjct: 363 FNASGERSESANVETAQVW----DDREQYFFYEVFPNFNEGSIAEMKSSIYESSQEGIRS 418
Query: 435 DPSHKKKELKKDSVKQKRWEQTQAKLAERKNRIKQKKASFI 557
+ KKE D +K + +++ R + K S +
Sbjct: 419 SSENNKKE---DDLKDSTGDLNTTQVSSRVDNFLLKLPSMV 456
>SPBC17D11.05 |tif32||translation initiation factor
eIF3a|Schizosaccharomyces pombe|chr 2|||Manual
Length = 932
Score = 27.5 bits (58), Expect = 1.6
Identities = 18/57 (31%), Positives = 29/57 (50%), Gaps = 1/57 (1%)
Frame = +3
Query: 396 EAIYKKAHEAIRADPSHKKKELKKDSVKQKRWEQTQAKLA-ERKNRIKQKKASFIKR 563
EAI + EA RA +EL + QKR + + + A E K R +++ A ++R
Sbjct: 788 EAIREAEEEAARAAEEEANRELHEQEEAQKRAIEERTRAAREAKEREQREMAEKLER 844
>SPCC162.08c |nup211||nuclear pore complex associated
protein|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1837
Score = 27.5 bits (58), Expect = 1.6
Identities = 13/54 (24%), Positives = 29/54 (53%)
Frame = +3
Query: 324 QDDEDSFKRQFSKYIKLGVTADAIEAIYKKAHEAIRADPSHKKKELKKDSVKQK 485
+++++S ++ +K ++ V +KK +E IR+D LK+D K++
Sbjct: 1023 KEEKESSSKELAKQLEDAVREKDSALSFKKDYEKIRSDADRVITSLKEDIEKER 1076
>SPCC63.14 |||conserved fungal protein|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1184
Score = 27.1 bits (57), Expect = 2.2
Identities = 16/54 (29%), Positives = 34/54 (62%), Gaps = 2/54 (3%)
Frame = +3
Query: 408 KKAHEAIRADPSHKKKELKKDSVKQKRW--EQTQAKLAERKNRIKQKKASFIKR 563
+ A E R + + + K LKK + ++KR ++ +A+ AE K R+++++A + K+
Sbjct: 708 RAAQERERKEQAKEAKRLKKLAKEEKRLKKKEEKARKAEEK-RLQKERAKYAKQ 760
Score = 25.8 bits (54), Expect = 5.0
Identities = 12/41 (29%), Positives = 24/41 (58%)
Frame = +3
Query: 408 KKAHEAIRADPSHKKKELKKDSVKQKRWEQTQAKLAERKNR 530
K+ + + + KKKE K ++KR ++ +AK A++ +R
Sbjct: 723 KRLKKLAKEEKRLKKKEEKARKAEEKRLQKERAKYAKQMSR 763
>SPBC646.10c |||U3 snoRNP protein Nop56 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 497
Score = 27.1 bits (57), Expect = 2.2
Identities = 16/45 (35%), Positives = 25/45 (55%), Gaps = 1/45 (2%)
Frame = +3
Query: 420 EAIRADPSHKKKELKKDSVKQKRWEQTQA-KLAERKNRIKQKKAS 551
E I PS K+K+ KK+ K+K ++ A +E + K+KK S
Sbjct: 452 ETISEKPSKKEKKDKKEKKKEKSKKKRSADDASEEVKKSKKKKKS 496
>SPCC330.19c |||sequence orphan|Schizosaccharomyces pombe|chr
3|||Manual
Length = 90
Score = 26.6 bits (56), Expect = 2.9
Identities = 13/34 (38%), Positives = 20/34 (58%), Gaps = 1/34 (2%)
Frame = -2
Query: 562 LLMKEAFFCLILFFLSANLA-CVCSQRFCLTESF 464
L++ AFFC ++FL LA C ++ C+T F
Sbjct: 39 LVLLFAFFCPCIYFLHTFLADCEIAKSTCITSVF 72
>SPBC1347.01c |rev1|SPBC215.16c|deoxycytidyl transferase Rev1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 935
Score = 26.6 bits (56), Expect = 2.9
Identities = 18/68 (26%), Positives = 33/68 (48%), Gaps = 6/68 (8%)
Frame = +3
Query: 243 RIQKFNAEVHRAHIFGLHVAEYMRSLEQD--DEDSFKRQFSKYIKLGV----TADAIEAI 404
RI+KFN + +GLH+ R ++ D + ++ +R S + GV D I+ +
Sbjct: 481 RIEKFNLQETFGVNYGLHLYNISRGIDTDIINNETPRRSISVDVNWGVRFVFQEDGIDFL 540
Query: 405 YKKAHEAI 428
+ HE +
Sbjct: 541 KRLLHELL 548
>SPBC11G11.05 |rpa34||DNA-directed RNA polymerase I complex subunit
Rpa34 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 251
Score = 26.6 bits (56), Expect = 2.9
Identities = 15/58 (25%), Positives = 28/58 (48%)
Frame = +3
Query: 369 KLGVTADAIEAIYKKAHEAIRADPSHKKKELKKDSVKQKRWEQTQAKLAERKNRIKQK 542
K G+ ++ E ++ K+KELKK ++K E+ K ++K+ K+K
Sbjct: 193 KSGIKEHILQETGDATVEELQNKGKEKQKELKKGKREKKDEEEKPKKKKQKKSSKKEK 250
>SPAC29A4.04c |||pseudouridylate synthase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 474
Score = 26.2 bits (55), Expect = 3.8
Identities = 11/40 (27%), Positives = 23/40 (57%)
Frame = +3
Query: 390 AIEAIYKKAHEAIRADPSHKKKELKKDSVKQKRWEQTQAK 509
++E+ K EA + + +KKE KK+ ++K ++ + K
Sbjct: 430 SVESSEKDEDEAAKKEEKRRKKEAKKEKKEKKEKKEKKEK 469
>SPAC890.05 |||ribosome biogenesis protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 284
Score = 26.2 bits (55), Expect = 3.8
Identities = 21/79 (26%), Positives = 36/79 (45%), Gaps = 2/79 (2%)
Frame = +3
Query: 321 EQDDEDSFKRQFSKYIKLGVTADA--IEAIYKKAHEAIRADPSHKKKELKKDSVKQKRWE 494
E+ + S K++ K G D +E I KK + HKKK+ + S K+K
Sbjct: 166 EKRKKHSSKKKSKKKTSTGSALDPKKLEKITKKKKK------EHKKKDKESSSKKRKSGS 219
Query: 495 QTQAKLAERKNRIKQKKAS 551
+ + ++K ++K K S
Sbjct: 220 SDKEEKKKKKIKLKDKPES 238
>SPAC23C4.17 |||tRNA |Schizosaccharomyces pombe|chr 1|||Manual
Length = 674
Score = 26.2 bits (55), Expect = 3.8
Identities = 15/28 (53%), Positives = 19/28 (67%)
Frame = +3
Query: 381 TADAIEAIYKKAHEAIRADPSHKKKELK 464
TA IEA+YKKA+ I+ D +H K LK
Sbjct: 174 TAQLIEAVYKKAN--IK-DAAHDSKNLK 198
>SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1085
Score = 25.8 bits (54), Expect = 5.0
Identities = 15/51 (29%), Positives = 31/51 (60%)
Frame = +3
Query: 396 EAIYKKAHEAIRADPSHKKKELKKDSVKQKRWEQTQAKLAERKNRIKQKKA 548
EA K+ + ++ + K++EL++ QKR E+ + K E+K + +Q++A
Sbjct: 636 EARKKREEQRLKREQEKKQQELER----QKREEKQKQKEREKKLKKQQQEA 682
>SPBC1709.02c |vas2|SPBC1734.18c|valine-tRNA ligase Vas2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 980
Score = 25.8 bits (54), Expect = 5.0
Identities = 12/38 (31%), Positives = 23/38 (60%)
Frame = +3
Query: 450 KKELKKDSVKQKRWEQTQAKLAERKNRIKQKKASFIKR 563
+KEL+++ K + E+ AKLA +K + + +K K+
Sbjct: 27 EKELERERQKAAKLEKYHAKLAAKKAKEEARKPKLDKK 64
>SPBP8B7.09c |||karyopherin|Schizosaccharomyces pombe|chr 2|||Manual
Length = 978
Score = 25.4 bits (53), Expect = 6.6
Identities = 10/24 (41%), Positives = 17/24 (70%)
Frame = +3
Query: 300 AEYMRSLEQDDEDSFKRQFSKYIK 371
+EY+++L D SFK+ F K+I+
Sbjct: 948 SEYLQALSNLDSRSFKQFFQKFIQ 971
>SPAC24B11.12c |||P-type ATPase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1402
Score = 25.0 bits (52), Expect = 8.8
Identities = 13/40 (32%), Positives = 22/40 (55%)
Frame = -3
Query: 126 QITSKCSWSIVDRFNIVFITCDICCASV*GVKSKSLKQSS 7
+IT +W++ F I+F C + CA V G+ + +SS
Sbjct: 446 RITRNLNWNVYLNFIILFSMCFV-CAVVEGIAWRGHSRSS 484
>SPBC4F6.07c |||ATP-dependent RNA helicase Mak5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 648
Score = 25.0 bits (52), Expect = 8.8
Identities = 13/37 (35%), Positives = 22/37 (59%), Gaps = 2/37 (5%)
Frame = +3
Query: 396 EAIYK--KAHEAIRADPSHKKKELKKDSVKQKRWEQT 500
E IY+ + HE P +KK+ K+ S K+K+ ++T
Sbjct: 48 EVIYELPEVHEKKDKKPEKRKKDQKEASEKKKKGKRT 84
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,241,502
Number of Sequences: 5004
Number of extensions: 43786
Number of successful extensions: 178
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 165
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 178
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 271646730
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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