BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30510 (738 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g10780.1 68418.m01253 expressed protein HSPC184, Homo sapiens... 44 8e-05 At1g33055.1 68414.m04073 expressed protein 30 1.8 At3g32070.1 68416.m04077 hypothetical protein 29 3.2 At4g06672.1 68417.m01069 hypothetical protein contains a non-con... 29 4.3 At2g04020.1 68415.m00369 GDSL-motif lipase/hydrolase family prot... 29 4.3 At5g57990.1 68418.m07255 ubiquitin-specific protease 23, putativ... 28 7.4 At2g04190.1 68415.m00404 meprin and TRAF homology domain-contain... 28 7.4 At5g35792.1 68418.m04296 hypothetical protein 27 9.8 At4g02660.1 68417.m00361 WD-40 repeat family protein / beige-rel... 27 9.8 >At5g10780.1 68418.m01253 expressed protein HSPC184, Homo sapiens, EMBL:AF151018 Length = 180 Score = 44.4 bits (100), Expect = 8e-05 Identities = 22/65 (33%), Positives = 39/65 (60%) Frame = +3 Query: 264 KDSDSNLLLIKKLWDVALGPLKQVPMNLFIMYMAGNSISIFPIMMVGMLIVRPVKALFAT 443 KD+++ L +K W+VA P K + M F+M+MAGN++ +F I + + +P+ AL + Sbjct: 53 KDAEATWKL-QKAWEVAQSPFKNLMMMGFMMWMAGNTVHLFSIGITFSALWQPISALQSV 111 Query: 444 QSTFK 458 F+ Sbjct: 112 GKIFE 116 Score = 35.9 bits (79), Expect = 0.028 Identities = 18/50 (36%), Positives = 27/50 (54%) Frame = +2 Query: 488 KNCLLYRNIVNILLALYKCQSMGLLPTHSSDGWHLKSLQQDSEHIGRGVI 637 K L N+ + L ++K ++GLLPTH+SD Q+ EH G G + Sbjct: 129 KLVFLALNLGGLALGVWKLNTLGLLPTHASDWVSSLPPPQEVEHSGGGFV 178 >At1g33055.1 68414.m04073 expressed protein Length = 66 Score = 29.9 bits (64), Expect = 1.8 Identities = 13/26 (50%), Positives = 17/26 (65%) Frame = +1 Query: 163 WALDFNQKNKQLSTELPSPPGYSQSS 240 ++L F+ N L LPSPP YS+SS Sbjct: 41 YSLPFHSSNTSLPLLLPSPPPYSRSS 66 >At3g32070.1 68416.m04077 hypothetical protein Length = 132 Score = 29.1 bits (62), Expect = 3.2 Identities = 11/22 (50%), Positives = 16/22 (72%) Frame = +3 Query: 252 CESSKDSDSNLLLIKKLWDVAL 317 CE KD ++L+ I+KLWD A+ Sbjct: 56 CEKFKDDRTDLMHIRKLWDKAM 77 >At4g06672.1 68417.m01069 hypothetical protein contains a non-consensus TT donor splice site, supported by homologous protein alignments. Length = 132 Score = 28.7 bits (61), Expect = 4.3 Identities = 11/22 (50%), Positives = 16/22 (72%) Frame = +3 Query: 252 CESSKDSDSNLLLIKKLWDVAL 317 CE KD ++L+ I+KLWD A+ Sbjct: 56 CERFKDDRNDLMHIRKLWDKAM 77 >At2g04020.1 68415.m00369 GDSL-motif lipase/hydrolase family protein similar to family II lipase EXL6 (GI:15054390), EXL1 (GI:15054382) [Arabidopsis thaliana]; contains Pfam profile PF00657: Lipase/Acylhydrolase with GDSL-like motif Length = 261 Score = 28.7 bits (61), Expect = 4.3 Identities = 13/22 (59%), Positives = 15/22 (68%) Frame = +1 Query: 436 LLHKVLSKWLKVHKLSDKKLFI 501 LLHK L K K+HKL +K FI Sbjct: 204 LLHKFLKKIEKLHKLGARKFFI 225 >At5g57990.1 68418.m07255 ubiquitin-specific protease 23, putative (UBP23) identical to GI:11993486 Length = 859 Score = 27.9 bits (59), Expect = 7.4 Identities = 21/91 (23%), Positives = 40/91 (43%), Gaps = 4/91 (4%) Frame = +2 Query: 326 ETSTHESFYYVHGW*LYLYISNNDGWHVNS-EAGEGAVCYTKYFQNG*RYTSYRTKNCL- 499 +T+ H+ + +V G+ I HV + G + K + R S +NC Sbjct: 136 QTAAHQKYCHVAGFCALCAIQK----HVRTARQANGRILAPKDLVSNLRCISRNFRNCRQ 191 Query: 500 --LYRNIVNILLALYKCQSMGLLPTHSSDGW 586 + ++N+L ++KC +P+ SSD + Sbjct: 192 EDAHEYMINLLECMHKCSLPSGVPSESSDAY 222 >At2g04190.1 68415.m00404 meprin and TRAF homology domain-containing protein / MATH domain-containing protein similar to ubiquitin-specific protease 12 [Arabidopsis thaliana] GI:11993471; contains Pfam profile PF00917: MATH domain Length = 411 Score = 27.9 bits (59), Expect = 7.4 Identities = 11/25 (44%), Positives = 17/25 (68%) Frame = +2 Query: 320 PFETSTHESFYYVHGW*LYLYISNN 394 P+E+S E+ Y+ H W L LY++ N Sbjct: 138 PYESSVFEA-YFEHKWRLILYVNGN 161 >At5g35792.1 68418.m04296 hypothetical protein Length = 132 Score = 27.5 bits (58), Expect = 9.8 Identities = 10/22 (45%), Positives = 16/22 (72%) Frame = +3 Query: 252 CESSKDSDSNLLLIKKLWDVAL 317 CE KD+ ++ + I+KLWD A+ Sbjct: 56 CEKFKDNRTDCMHIRKLWDKAI 77 >At4g02660.1 68417.m00361 WD-40 repeat family protein / beige-related contains Pfam PF00400: WD domain, G-beta repeat; similar to BEIGE (GI:3928547) [Rattus norvegicus]; lysosomal trafficking regulator - Bos taurus, EMBL: AF114785 Length = 3471 Score = 27.5 bits (58), Expect = 9.8 Identities = 12/28 (42%), Positives = 17/28 (60%) Frame = -3 Query: 391 IGNIEIELPAMYIIKRFMGTCFKGPNAT 308 +GN+ I+LP +I F GTC + AT Sbjct: 1290 LGNLSIQLPGKKLIFAFDGTCSEFMRAT 1317 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 16,214,578 Number of Sequences: 28952 Number of extensions: 360312 Number of successful extensions: 898 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 859 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 895 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1624036432 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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