Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30506
(597 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 150 1e-37
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 150 2e-37
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 150 2e-37
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 94 2e-20
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 89 5e-19
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 56 3e-09
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 30 0.29
SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 29 0.68
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 26 3.6
SPCC364.02c |bis1||stress response protein Bis1|Schizosaccharomy... 26 4.8
SPBC1703.06 |pof10||F-box protein Pof10|Schizosaccharomyces pomb... 25 6.3
SPBC2D10.18 |abc1|coq8|ABC1 kinase family protein|Schizosaccharo... 25 8.4
SPBC1703.13c |||inorganic phosphate transporter |Schizosaccharom... 25 8.4
SPBC29A3.14c |trt1||telomerase reverse transcriptase 1 protein T... 25 8.4
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 25 8.4
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 25 8.4
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 150 bits (364), Expect = 1e-37
Identities = 72/99 (72%), Positives = 86/99 (86%)
Frame = +2
Query: 245 FHQDMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDS 424
F ++MITGTSQADCAVLI+ GTGEFEAGISK+GQTREHALLA+TLGVKQLIV VNKMD+
Sbjct: 98 FIKNMITGTSQADCAVLIIGGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDT 157
Query: 425 TEPPYSEPRFEEIKKEVSSYIKKIGYNPLAVAFVPISGW 541
T +S+ RFEEI KE S++IKK+G+NP V FVP+SG+
Sbjct: 158 T--GWSQARFEEIVKETSNFIKKVGFNPKTVPFVPVSGF 194
Score = 112 bits (270), Expect = 3e-26
Identities = 51/53 (96%), Positives = 52/53 (98%)
Frame = +1
Query: 1 VDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 159
VDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA E+GKGSFKYAWVLDKLKAERE
Sbjct: 16 VDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVLDKLKAERE 68
Score = 64.9 bits (151), Expect = 8e-12
Identities = 28/31 (90%), Positives = 29/31 (93%)
Frame = +3
Query: 162 GITIDIALWKFETSKYYVTIIDAPGHRDFIK 254
GITIDIALWKFET KY VT+IDAPGHRDFIK
Sbjct: 70 GITIDIALWKFETPKYNVTVIDAPGHRDFIK 100
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 150 bits (363), Expect = 2e-37
Identities = 71/99 (71%), Positives = 86/99 (86%)
Frame = +2
Query: 245 FHQDMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDS 424
F ++MITGTSQADCA+LI+ GTGEFEAGISK+GQTREHALLA+TLGVKQLIV VNKMD+
Sbjct: 98 FIKNMITGTSQADCAILIIGGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDT 157
Query: 425 TEPPYSEPRFEEIKKEVSSYIKKIGYNPLAVAFVPISGW 541
T +S+ RFEEI KE S++IKK+G+NP V FVP+SG+
Sbjct: 158 T--GWSQARFEEIVKETSNFIKKVGFNPKTVPFVPVSGF 194
Score = 112 bits (270), Expect = 3e-26
Identities = 51/53 (96%), Positives = 52/53 (98%)
Frame = +1
Query: 1 VDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 159
VDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA E+GKGSFKYAWVLDKLKAERE
Sbjct: 16 VDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVLDKLKAERE 68
Score = 64.9 bits (151), Expect = 8e-12
Identities = 28/31 (90%), Positives = 29/31 (93%)
Frame = +3
Query: 162 GITIDIALWKFETSKYYVTIIDAPGHRDFIK 254
GITIDIALWKFET KY VT+IDAPGHRDFIK
Sbjct: 70 GITIDIALWKFETPKYNVTVIDAPGHRDFIK 100
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 150 bits (363), Expect = 2e-37
Identities = 71/99 (71%), Positives = 86/99 (86%)
Frame = +2
Query: 245 FHQDMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDS 424
F ++MITGTSQADCA+LI+ GTGEFEAGISK+GQTREHALLA+TLGVKQLIV VNKMD+
Sbjct: 98 FIKNMITGTSQADCAILIIGGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDT 157
Query: 425 TEPPYSEPRFEEIKKEVSSYIKKIGYNPLAVAFVPISGW 541
T +S+ RFEEI KE S++IKK+G+NP V FVP+SG+
Sbjct: 158 T--GWSQARFEEIVKETSNFIKKVGFNPKTVPFVPVSGF 194
Score = 112 bits (270), Expect = 3e-26
Identities = 51/53 (96%), Positives = 52/53 (98%)
Frame = +1
Query: 1 VDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 159
VDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA E+GKGSFKYAWVLDKLKAERE
Sbjct: 16 VDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVLDKLKAERE 68
Score = 64.9 bits (151), Expect = 8e-12
Identities = 28/31 (90%), Positives = 29/31 (93%)
Frame = +3
Query: 162 GITIDIALWKFETSKYYVTIIDAPGHRDFIK 254
GITIDIALWKFET KY VT+IDAPGHRDFIK
Sbjct: 70 GITIDIALWKFETPKYNVTVIDAPGHRDFIK 100
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 93.9 bits (223), Expect = 2e-20
Identities = 44/98 (44%), Positives = 67/98 (68%), Gaps = 2/98 (2%)
Frame = +2
Query: 254 DMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEP 433
+MI G SQAD VL+++A GEFEAG + GQTREHA+LA T G+ L+V +NKMD
Sbjct: 332 NMINGASQADIGVLVISARRGEFEAGFERGGQTREHAVLARTQGINHLVVVINKMDEPSV 391
Query: 434 PYSEPRFEEIKKEVSSYIKKI-GYNPLA-VAFVPISGW 541
+SE R++E ++S +++++ GYN V ++P+S +
Sbjct: 392 QWSEERYKECVDKLSMFLRRVAGYNSKTDVKYMPVSAY 429
Score = 62.1 bits (144), Expect = 6e-11
Identities = 27/53 (50%), Positives = 38/53 (71%)
Frame = +1
Query: 1 VDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 159
VD+GKST G++++ G +DKRT+EK E+EA+E GK S+ +W LD ERE
Sbjct: 247 VDAGKSTLGGNILFLTGMVDKRTMEKIEREAKEAGKESWYLSWALDSTSEERE 299
Score = 29.1 bits (62), Expect = 0.52
Identities = 10/30 (33%), Positives = 19/30 (63%)
Frame = +3
Query: 162 GITIDIALWKFETSKYYVTIIDAPGHRDFI 251
G T+++ FET +++DAPGH+ ++
Sbjct: 301 GKTVEVGRAYFETEHRRFSLLDAPGHKGYV 330
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 89.0 bits (211), Expect = 5e-19
Identities = 48/98 (48%), Positives = 61/98 (62%), Gaps = 1/98 (1%)
Frame = +2
Query: 245 FHQDMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDS 424
F MI G S AD AVL+V + FE G +NGQTREHA L LG+ +++V VNK+D
Sbjct: 268 FISGMIAGASSADFAVLVVDSSQNNFERGFLENGQTREHAYLLRALGISEIVVSVNKLDL 327
Query: 425 TEPPYSEPRFEEIKKEVSSY-IKKIGYNPLAVAFVPIS 535
+SE RF+EIK VS + IK +G+ V FVPIS
Sbjct: 328 MS--WSEDRFQEIKNIVSDFLIKMVGFKTSNVHFVPIS 363
Score = 61.3 bits (142), Expect = 1e-10
Identities = 26/52 (50%), Positives = 36/52 (69%)
Frame = +1
Query: 1 VDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAER 156
VDSGKST G ++++ G I+ R+++K EA GKGSF YAW+LD + ER
Sbjct: 186 VDSGKSTMLGRIMFELGEINSRSMQKLHNEAANSGKGSFSYAWLLDTTEEER 237
Score = 39.1 bits (87), Expect = 5e-04
Identities = 17/30 (56%), Positives = 21/30 (70%)
Frame = +3
Query: 162 GITIDIALWKFETSKYYVTIIDAPGHRDFI 251
G+T+D+A FE+ K I DAPGHRDFI
Sbjct: 240 GVTMDVASTTFESDKKIYEIGDAPGHRDFI 269
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 56.4 bits (130), Expect = 3e-09
Identities = 33/89 (37%), Positives = 51/89 (57%)
Frame = +2
Query: 218 HH*CSWTQRFHQDMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQL 397
H C + ++MITG + D A+++V+A G+ QTREH LLA +GVKQ+
Sbjct: 120 HVDCPGHADYIKNMITGAATMDGAIIVVSATDGQMP-------QTREHLLLARQVGVKQI 172
Query: 398 IVGVNKMDSTEPPYSEPRFEEIKKEVSSY 484
+V +NK+D EP E E+++ +S Y
Sbjct: 173 VVYINKVDMVEPDMIELVEMEMRELLSEY 201
Score = 33.5 bits (73), Expect = 0.024
Identities = 14/31 (45%), Positives = 20/31 (64%)
Frame = +3
Query: 162 GITIDIALWKFETSKYYVTIIDAPGHRDFIK 254
GITI A ++ET+ + +D PGH D+IK
Sbjct: 101 GITISSAHVEYETANRHYAHVDCPGHADYIK 131
>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 813
Score = 29.9 bits (64), Expect = 0.29
Identities = 13/29 (44%), Positives = 16/29 (55%)
Frame = +3
Query: 162 GITIDIALWKFETSKYYVTIIDAPGHRDF 248
GITI+ A F + +ID PGH DF
Sbjct: 78 GITINSAAISFTWRNQRINLIDTPGHADF 106
>SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 446
Score = 28.7 bits (61), Expect = 0.68
Identities = 18/77 (23%), Positives = 33/77 (42%)
Frame = +2
Query: 257 MITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPP 436
M+ G + D A+L++A QT EH + +K +I+ NK+D
Sbjct: 125 MLNGAAVMDAALLLIAGNES------CPQPQTSEHLAAIEIMQLKHIIILQNKVDLIRES 178
Query: 437 YSEPRFEEIKKEVSSYI 487
+E ++ I K + +
Sbjct: 179 AAEEHYQSILKFIKGTV 195
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 26.2 bits (55), Expect = 3.6
Identities = 12/19 (63%), Positives = 13/19 (68%)
Frame = +3
Query: 192 FETSKYYVTIIDAPGHRDF 248
FE S Y + IID PGH DF
Sbjct: 141 FEKS-YNINIIDTPGHIDF 158
>SPCC364.02c |bis1||stress response protein Bis1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 384
Score = 25.8 bits (54), Expect = 4.8
Identities = 18/60 (30%), Positives = 28/60 (46%)
Frame = -2
Query: 230 SINDGNIVLASFELPESNIDCDTDSRSAFSLSNTQAYLKDPLPISWASFSNFSMVRLSIP 51
S++ + L S+ P S T+ A ++YL+ PL S S S + +R SIP
Sbjct: 305 SVDSASTALNSYSTPNSVSRKLTNLTPAARRLVARSYLRSPLHGSSPSASRHTALRTSIP 364
>SPBC1703.06 |pof10||F-box protein Pof10|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 662
Score = 25.4 bits (53), Expect = 6.3
Identities = 15/43 (34%), Positives = 19/43 (44%)
Frame = -3
Query: 142 VCPIPKHI*RILYPFPGPPSRTSRWYVCQYHHICRSSDQWWWT 14
V +PK I I++ F P S S C+Y S D W T
Sbjct: 31 VLNLPKEILIIIFSFLDPRSLLSAQCTCKYWKKLLSDDLSWRT 73
>SPBC2D10.18 |abc1|coq8|ABC1 kinase family
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 610
Score = 25.0 bits (52), Expect = 8.4
Identities = 10/26 (38%), Positives = 17/26 (65%)
Frame = +2
Query: 509 LAVAFVPISGWARRQHVGSLSTKIAL 586
L+ + VP S W+R H G L+T +++
Sbjct: 174 LSSSKVPSSQWSRLWHYGGLATSLSV 199
>SPBC1703.13c |||inorganic phosphate transporter
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 311
Score = 25.0 bits (52), Expect = 8.4
Identities = 8/19 (42%), Positives = 12/19 (63%)
Frame = -3
Query: 97 PGPPSRTSRWYVCQYHHIC 41
P PP +T + Y QY+ +C
Sbjct: 8 PAPPKKTLQLYTPQYYGLC 26
>SPBC29A3.14c |trt1||telomerase reverse transcriptase 1 protein Trt1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 988
Score = 25.0 bits (52), Expect = 8.4
Identities = 19/62 (30%), Positives = 30/62 (48%), Gaps = 7/62 (11%)
Frame = +3
Query: 414 KWIPL-NHHTVSPDLRKSRRKYPHTSRRLATTHWLSLS------CPFLDGHGDNMLGAFQ 572
K IPL + TV P ++ + YP + H +SLS CP++D H D + ++
Sbjct: 280 KVIPLVSQSTVVP--KRLLKVYPLIEQTAKRLHRISLSKVYNHYCPYIDTHDDEKILSYS 337
Query: 573 PK 578
K
Sbjct: 338 LK 339
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 25.0 bits (52), Expect = 8.4
Identities = 8/22 (36%), Positives = 13/22 (59%)
Frame = +3
Query: 183 LWKFETSKYYVTIIDAPGHRDF 248
++ + Y + +ID PGH DF
Sbjct: 116 IYYYHGQSYLLNLIDTPGHVDF 137
>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 1000
Score = 25.0 bits (52), Expect = 8.4
Identities = 8/14 (57%), Positives = 11/14 (78%)
Frame = +3
Query: 207 YYVTIIDAPGHRDF 248
Y + +ID+PGH DF
Sbjct: 94 YLINLIDSPGHVDF 107
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,647,036
Number of Sequences: 5004
Number of extensions: 55717
Number of successful extensions: 175
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 158
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 170
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 260219058
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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