BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30506 (597 letters) Database: bee 438 sequences; 146,343 total letters Searching......................................................done Score E Sequences producing significant alignments: (bits) Value EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 195 2e-52 AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 195 2e-52 AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 195 2e-52 X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 185 3e-49 AF069739-1|AAC63272.2| 690|Apis mellifera translation initiatio... 22 4.0 AB253416-1|BAE86927.1| 580|Apis mellifera alpha-glucosidase pro... 21 6.9 DQ855484-1|ABH88171.1| 130|Apis mellifera chemosensory protein ... 21 9.2 AY937243-1|AAX33677.1| 1370|Apis mellifera Toll-like receptor pr... 21 9.2 AJ973401-1|CAJ01448.1| 130|Apis mellifera hypothetical protein ... 21 9.2 AF481963-1|AAN59784.1| 130|Apis mellifera antennal-specific pro... 21 9.2 AB253415-1|BAE86926.1| 588|Apis mellifera alpha-glucosidase pro... 21 9.2 >EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-alpha protein. Length = 172 Score = 195 bits (476), Expect = 2e-52 Identities = 94/99 (94%), Positives = 96/99 (96%) Frame = +2 Query: 245 FHQDMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDS 424 F ++MITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDS Sbjct: 25 FIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDS 84 Query: 425 TEPPYSEPRFEEIKKEVSSYIKKIGYNPLAVAFVPISGW 541 TEPPYSE RFEEIKKEVSSYIKKIGYNP AVAFVPISGW Sbjct: 85 TEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPISGW 123 Score = 63.3 bits (147), Expect = 2e-12 Identities = 27/27 (100%), Positives = 27/27 (100%) Frame = +3 Query: 174 DIALWKFETSKYYVTIIDAPGHRDFIK 254 DIALWKFETSKYYVTIIDAPGHRDFIK Sbjct: 1 DIALWKFETSKYYVTIIDAPGHRDFIK 27 Score = 30.3 bits (65), Expect = 0.015 Identities = 13/23 (56%), Positives = 14/23 (60%) Frame = +3 Query: 528 PFLDGHGDNMLGAFQPKLPWFKG 596 P HGDNML K+PWFKG Sbjct: 119 PISGWHGDNMLEV-SSKMPWFKG 140 >AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-alpha protein. Length = 274 Score = 195 bits (476), Expect = 2e-52 Identities = 94/99 (94%), Positives = 96/99 (96%) Frame = +2 Query: 245 FHQDMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDS 424 F ++MITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDS Sbjct: 41 FIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDS 100 Query: 425 TEPPYSEPRFEEIKKEVSSYIKKIGYNPLAVAFVPISGW 541 TEPPYSE RFEEIKKEVSSYIKKIGYNP AVAFVPISGW Sbjct: 101 TEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPISGW 139 Score = 70.9 bits (166), Expect = 9e-15 Identities = 31/31 (100%), Positives = 31/31 (100%) Frame = +3 Query: 162 GITIDIALWKFETSKYYVTIIDAPGHRDFIK 254 GITIDIALWKFETSKYYVTIIDAPGHRDFIK Sbjct: 13 GITIDIALWKFETSKYYVTIIDAPGHRDFIK 43 Score = 30.3 bits (65), Expect = 0.015 Identities = 13/23 (56%), Positives = 14/23 (60%) Frame = +3 Query: 528 PFLDGHGDNMLGAFQPKLPWFKG 596 P HGDNML K+PWFKG Sbjct: 135 PISGWHGDNMLEV-SSKMPWFKG 156 Score = 27.5 bits (58), Expect = 0.11 Identities = 11/11 (100%), Positives = 11/11 (100%) Frame = +1 Query: 127 WVLDKLKAERE 159 WVLDKLKAERE Sbjct: 1 WVLDKLKAERE 11 >AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha F2 protein. Length = 461 Score = 195 bits (476), Expect = 2e-52 Identities = 94/99 (94%), Positives = 96/99 (96%) Frame = +2 Query: 245 FHQDMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDS 424 F ++MITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDS Sbjct: 98 FIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDS 157 Query: 425 TEPPYSEPRFEEIKKEVSSYIKKIGYNPLAVAFVPISGW 541 TEPPYSE RFEEIKKEVSSYIKKIGYNP AVAFVPISGW Sbjct: 158 TEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPISGW 196 Score = 116 bits (279), Expect = 2e-28 Identities = 53/53 (100%), Positives = 53/53 (100%) Frame = +1 Query: 1 VDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 159 VDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE Sbjct: 16 VDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 68 Score = 70.9 bits (166), Expect = 9e-15 Identities = 31/31 (100%), Positives = 31/31 (100%) Frame = +3 Query: 162 GITIDIALWKFETSKYYVTIIDAPGHRDFIK 254 GITIDIALWKFETSKYYVTIIDAPGHRDFIK Sbjct: 70 GITIDIALWKFETSKYYVTIIDAPGHRDFIK 100 Score = 30.3 bits (65), Expect = 0.015 Identities = 13/23 (56%), Positives = 14/23 (60%) Frame = +3 Query: 528 PFLDGHGDNMLGAFQPKLPWFKG 596 P HGDNML K+PWFKG Sbjct: 192 PISGWHGDNMLEV-SSKMPWFKG 213 >X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha protein. Length = 461 Score = 185 bits (451), Expect = 3e-49 Identities = 89/99 (89%), Positives = 93/99 (93%) Frame = +2 Query: 245 FHQDMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDS 424 F ++MITGTSQADCAVLIVAAG GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMD Sbjct: 98 FIKNMITGTSQADCAVLIVAAGIGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDM 157 Query: 425 TEPPYSEPRFEEIKKEVSSYIKKIGYNPLAVAFVPISGW 541 T+PPYSE RFEEIKKEVSSYIKKIGYN +VAFVPISGW Sbjct: 158 TDPPYSEARFEEIKKEVSSYIKKIGYNTASVAFVPISGW 196 Score = 116 bits (279), Expect = 2e-28 Identities = 53/53 (100%), Positives = 53/53 (100%) Frame = +1 Query: 1 VDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 159 VDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE Sbjct: 16 VDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 68 Score = 69.7 bits (163), Expect = 2e-14 Identities = 30/31 (96%), Positives = 31/31 (100%) Frame = +3 Query: 162 GITIDIALWKFETSKYYVTIIDAPGHRDFIK 254 GITIDIALWKFET+KYYVTIIDAPGHRDFIK Sbjct: 70 GITIDIALWKFETAKYYVTIIDAPGHRDFIK 100 Score = 30.7 bits (66), Expect = 0.011 Identities = 13/23 (56%), Positives = 14/23 (60%) Frame = +3 Query: 528 PFLDGHGDNMLGAFQPKLPWFKG 596 P HGDNML PK PW+KG Sbjct: 192 PISGWHGDNMLEP-SPKTPWYKG 213 >AF069739-1|AAC63272.2| 690|Apis mellifera translation initiation factor 2 protein. Length = 690 Score = 22.2 bits (45), Expect = 4.0 Identities = 8/13 (61%), Positives = 9/13 (69%) Frame = +3 Query: 213 VTIIDAPGHRDFI 251 VT +D PGH FI Sbjct: 195 VTFLDTPGHAAFI 207 >AB253416-1|BAE86927.1| 580|Apis mellifera alpha-glucosidase protein. Length = 580 Score = 21.4 bits (43), Expect = 6.9 Identities = 7/20 (35%), Positives = 13/20 (65%) Frame = +3 Query: 417 WIPLNHHTVSPDLRKSRRKY 476 W+P+N + S +L +R+Y Sbjct: 443 WLPVNENYKSLNLAAQKREY 462 >DQ855484-1|ABH88171.1| 130|Apis mellifera chemosensory protein 3 protein. Length = 130 Score = 21.0 bits (42), Expect = 9.2 Identities = 8/16 (50%), Positives = 13/16 (81%) Frame = +1 Query: 58 DKRTIEKFEKEAQEMG 105 DK+ KFE+EA+++G Sbjct: 112 DKKYRVKFEEEAKKLG 127 >AY937243-1|AAX33677.1| 1370|Apis mellifera Toll-like receptor protein. Length = 1370 Score = 21.0 bits (42), Expect = 9.2 Identities = 12/29 (41%), Positives = 14/29 (48%) Frame = -3 Query: 358 LTGLTVLRDTSFEFTGTGSYDEHSAISLR 272 LTGLTVLR + + S E S R Sbjct: 257 LTGLTVLRTFNASYNSLDSLPEGLFASTR 285 >AJ973401-1|CAJ01448.1| 130|Apis mellifera hypothetical protein protein. Length = 130 Score = 21.0 bits (42), Expect = 9.2 Identities = 8/16 (50%), Positives = 13/16 (81%) Frame = +1 Query: 58 DKRTIEKFEKEAQEMG 105 DK+ KFE+EA+++G Sbjct: 112 DKKFRVKFEEEAKKLG 127 >AF481963-1|AAN59784.1| 130|Apis mellifera antennal-specific protein 3c precursor protein. Length = 130 Score = 21.0 bits (42), Expect = 9.2 Identities = 8/16 (50%), Positives = 13/16 (81%) Frame = +1 Query: 58 DKRTIEKFEKEAQEMG 105 DK+ KFE+EA+++G Sbjct: 112 DKKYRVKFEEEAKKLG 127 >AB253415-1|BAE86926.1| 588|Apis mellifera alpha-glucosidase protein. Length = 588 Score = 21.0 bits (42), Expect = 9.2 Identities = 8/18 (44%), Positives = 12/18 (66%) Frame = -3 Query: 469 LLDFLKSGLTVWWFSGIH 416 L F++SG+T W S I+ Sbjct: 56 LSHFIESGITAIWLSPIN 73 Database: bee Posted date: Oct 23, 2007 1:17 PM Number of letters in database: 146,343 Number of sequences in database: 438 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 183,720 Number of Sequences: 438 Number of extensions: 3819 Number of successful extensions: 23 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 12 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 23 length of database: 146,343 effective HSP length: 55 effective length of database: 122,253 effective search space used: 17482179 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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