BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30504 (662 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gc... 28 1.4 SPBC215.07c |||PWWP domain protein|Schizosaccharomyces pombe|chr... 27 3.2 SPCC364.07 ||SPCC4G3.01|D-3 phosphoglycerate dehydrogenase |Schi... 27 3.2 SPBC31E1.05 |gle1||RNA export factor Gle1 |Schizosaccharomyces p... 27 3.2 SPCC645.05c |myo2|rng5|myosin II heavy chain|Schizosaccharomyces... 27 3.2 SPCC1739.14 |npp106||nucleoporin Npp106|Schizosaccharomyces pomb... 26 5.6 SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1||... 26 5.6 SPCC550.14 |||vigilin |Schizosaccharomyces pombe|chr 3|||Manual 26 5.6 SPAC20G4.07c |sts1|erg4|C-24|Schizosaccharomyces pombe|chr 1|||M... 25 7.4 SPBC30B4.08 |eri1||double-strand siRNA ribonuclease|Schizosaccha... 25 7.4 SPBC691.03c |apl3||AP-2 adaptor complex subunit Alp3 |Schizosacc... 25 7.4 SPBC28E12.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2|... 25 9.7 >SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gcn2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1576 Score = 27.9 bits (59), Expect = 1.4 Identities = 13/34 (38%), Positives = 20/34 (58%) Frame = +2 Query: 122 SRDSTSVLQQMSTLIKSRKERNLPLDNLLALIIH 223 S DS S+ Q ++ + R+ +LP D L +L IH Sbjct: 635 SSDSESLSQSLNMAVDFRQSSSLPADKLSSLDIH 668 >SPBC215.07c |||PWWP domain protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 568 Score = 26.6 bits (56), Expect = 3.2 Identities = 15/57 (26%), Positives = 27/57 (47%), Gaps = 5/57 (8%) Frame = +3 Query: 378 SKNIEATKGNSIDEDKFA-----EIQLVNKDKRDRNRARIPRPVTAASRDLVDTDRP 533 SKNIE T N ++ K + L +K+ D++ + + R ++TD+P Sbjct: 36 SKNIETTSSNGAEDIKIQRDVGDDKDLDDKEANDKSSKGLEEESDSKDRKTIETDQP 92 >SPCC364.07 ||SPCC4G3.01|D-3 phosphoglycerate dehydrogenase |Schizosaccharomyces pombe|chr 3|||Manual Length = 466 Score = 26.6 bits (56), Expect = 3.2 Identities = 12/28 (42%), Positives = 17/28 (60%) Frame = +3 Query: 399 KGNSIDEDKFAEIQLVNKDKRDRNRARI 482 +GNSI F E+ L + + DRN AR+ Sbjct: 370 EGNSIGAVNFPEVSLRSLTEADRNAARV 397 >SPBC31E1.05 |gle1||RNA export factor Gle1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 480 Score = 26.6 bits (56), Expect = 3.2 Identities = 11/34 (32%), Positives = 22/34 (64%) Frame = +3 Query: 381 KNIEATKGNSIDEDKFAEIQLVNKDKRDRNRARI 482 K IEA + DE++ + +L+ K+K+++ R R+ Sbjct: 121 KRIEAERQRLKDEEERRKKELMEKEKKEKERIRL 154 >SPCC645.05c |myo2|rng5|myosin II heavy chain|Schizosaccharomyces pombe|chr 3|||Manual Length = 1526 Score = 26.6 bits (56), Expect = 3.2 Identities = 12/24 (50%), Positives = 17/24 (70%) Frame = +1 Query: 370 EISQKTLKRLKEIALMRINLQKYN 441 +I QK LK ++ I L++ NLQ YN Sbjct: 776 KIFQKRLKDIQAIKLLQANLQVYN 799 >SPCC1739.14 |npp106||nucleoporin Npp106|Schizosaccharomyces pombe|chr 3|||Manual Length = 933 Score = 25.8 bits (54), Expect = 5.6 Identities = 12/29 (41%), Positives = 21/29 (72%) Frame = -3 Query: 198 SKGKLRSLRDFINVLICCKTLVESRLAPK 112 SK KL+ L+ ++L+ T++ESRL+P+ Sbjct: 892 SKKKLQRLQFKGSMLVMFSTMIESRLSPQ 920 >SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1|||Manual Length = 3227 Score = 25.8 bits (54), Expect = 5.6 Identities = 14/56 (25%), Positives = 29/56 (51%) Frame = +2 Query: 104 LQNLGASRDSTSVLQQMSTLIKSRKERNLPLDNLLALIIHIYSLGGTEVTSQKLMN 271 LQ++ + + + ++ M I +R L NLL ++H+ GG+ V + L++ Sbjct: 471 LQDIKSGKGNPEIVLNMDYAISY--DRYFLLKNLLKFVLHLIQSGGSVVELRNLID 524 >SPCC550.14 |||vigilin |Schizosaccharomyces pombe|chr 3|||Manual Length = 1279 Score = 25.8 bits (54), Expect = 5.6 Identities = 11/33 (33%), Positives = 19/33 (57%) Frame = +1 Query: 268 EQDLIEALSVAMFEDHKSLFPQVEGHIVTPEEC 366 +++++EAL + E + L QVE I P+ C Sbjct: 1017 DKEIVEALETRLLEIVEELKNQVEEKIEVPQRC 1049 >SPAC20G4.07c |sts1|erg4|C-24|Schizosaccharomyces pombe|chr 1|||Manual Length = 453 Score = 25.4 bits (53), Expect = 7.4 Identities = 9/22 (40%), Positives = 16/22 (72%) Frame = -2 Query: 616 ISCLVKMFKPLLSKSLIPSFLC 551 I+ + MF PL+S ++I +F+C Sbjct: 140 ITTFIDMFGPLMSVAIITAFVC 161 >SPBC30B4.08 |eri1||double-strand siRNA ribonuclease|Schizosaccharomyces pombe|chr 2|||Manual Length = 313 Score = 25.4 bits (53), Expect = 7.4 Identities = 14/30 (46%), Positives = 20/30 (66%) Frame = +1 Query: 346 IVTPEECDEISQKTLKRLKEIALMRINLQK 435 +V P CDE++QKT ++EI RI LQ+ Sbjct: 8 LVWPFPCDEMNQKTPSTVEEI---RIALQE 34 >SPBC691.03c |apl3||AP-2 adaptor complex subunit Alp3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 878 Score = 25.4 bits (53), Expect = 7.4 Identities = 15/70 (21%), Positives = 28/70 (40%) Frame = +2 Query: 20 QLALAVVQTLKSPRRAQLELLASLEKQVLQNLGASRDSTSVLQQMSTLIKSRKERNLPLD 199 +L V+ ++K + L SL + N+G +V + L+ S N+ Sbjct: 102 ELMKLVINSIKKDLLSHDSLQNSLALHTIANIGGRELCETVYYDIYKLLMSASNENIVRQ 161 Query: 200 NLLALIIHIY 229 ++HIY Sbjct: 162 KSALALLHIY 171 >SPBC28E12.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 356 Score = 25.0 bits (52), Expect = 9.7 Identities = 14/45 (31%), Positives = 27/45 (60%) Frame = +1 Query: 205 ARVNHSHILSRRY*SHISKTHEQDLIEALSVAMFEDHKSLFPQVE 339 +++ + L +R+ S + +T L+ SV+++ED SLFP+ E Sbjct: 83 SKITKTRSLLKRFGSCLDETSTIVLLNQ-SVSLWEDCYSLFPKTE 126 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,729,143 Number of Sequences: 5004 Number of extensions: 55357 Number of successful extensions: 157 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 150 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 157 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 301829700 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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