Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30503
(681 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 169 3e-43
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 169 4e-43
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 169 4e-43
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 101 7e-23
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 90 2e-19
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 62 1e-10
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 46 7e-06
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 41 1e-04
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 36 0.007
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 36 0.007
SPBC1271.15c |||translation initiation factor IF-2Mt|Schizosacch... 34 0.022
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 33 0.029
SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 31 0.15
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 30 0.27
SPAC56F8.03 |||translation initiation factor IF2 |Schizosaccharo... 29 0.47
SPAC328.01c ||SPAC3A11.01|karyopherin|Schizosaccharomyces pombe|... 29 0.62
SPAC9G1.11c |spn4||septin Spn4|Schizosaccharomyces pombe|chr 1||... 28 1.1
SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 27 1.9
SPAC23E2.03c |ste7||meiotic suppressor protein Ste7|Schizosaccha... 26 5.8
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 169 bits (411), Expect = 3e-43
Identities = 81/97 (83%), Positives = 84/97 (86%)
Frame = +2
Query: 251 G*GSFKYAWVLDKLKAERELGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQA 430
G GSFKYAWVLDKLKAERE GITIDIALWKFET KY VT+IDAPGHRDFIKNMITGTSQA
Sbjct: 50 GKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQA 109
Query: 431 DCAVLIVAAGTGEFEAGISKNGQTREMPCSLSPLGVK 541
DCAVLI+ GTGEFEAGISK+GQTRE LGVK
Sbjct: 110 DCAVLIIGGGTGEFEAGISKDGQTREHALLAYTLGVK 146
Score = 105 bits (251), Expect = 8e-24
Identities = 47/51 (92%), Positives = 49/51 (96%)
Frame = +3
Query: 105 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGK 257
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA E+GK
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGK 51
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 169 bits (410), Expect = 4e-43
Identities = 80/97 (82%), Positives = 84/97 (86%)
Frame = +2
Query: 251 G*GSFKYAWVLDKLKAERELGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQA 430
G GSFKYAWVLDKLKAERE GITIDIALWKFET KY VT+IDAPGHRDFIKNMITGTSQA
Sbjct: 50 GKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQA 109
Query: 431 DCAVLIVAAGTGEFEAGISKNGQTREMPCSLSPLGVK 541
DCA+LI+ GTGEFEAGISK+GQTRE LGVK
Sbjct: 110 DCAILIIGGGTGEFEAGISKDGQTREHALLAYTLGVK 146
Score = 105 bits (251), Expect = 8e-24
Identities = 47/51 (92%), Positives = 49/51 (96%)
Frame = +3
Query: 105 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGK 257
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA E+GK
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGK 51
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 169 bits (410), Expect = 4e-43
Identities = 80/97 (82%), Positives = 84/97 (86%)
Frame = +2
Query: 251 G*GSFKYAWVLDKLKAERELGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQA 430
G GSFKYAWVLDKLKAERE GITIDIALWKFET KY VT+IDAPGHRDFIKNMITGTSQA
Sbjct: 50 GKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQA 109
Query: 431 DCAVLIVAAGTGEFEAGISKNGQTREMPCSLSPLGVK 541
DCA+LI+ GTGEFEAGISK+GQTRE LGVK
Sbjct: 110 DCAILIIGGGTGEFEAGISKDGQTREHALLAYTLGVK 146
Score = 105 bits (251), Expect = 8e-24
Identities = 47/51 (92%), Positives = 49/51 (96%)
Frame = +3
Query: 105 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGK 257
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA E+GK
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGK 51
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 101 bits (243), Expect = 7e-23
Identities = 50/97 (51%), Positives = 61/97 (62%)
Frame = +2
Query: 248 NG*GSFKYAWVLDKLKAERELGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQ 427
+G GSF YAW+LD + ER G+T+D+A FE+ K I DAPGHRDFI MI G S
Sbjct: 219 SGKGSFSYAWLLDTTEEERARGVTMDVASTTFESDKKIYEIGDAPGHRDFISGMIAGASS 278
Query: 428 ADCAVLIVAAGTGEFEAGISKNGQTREMPCSLSPLGV 538
AD AVL+V + FE G +NGQTRE L LG+
Sbjct: 279 ADFAVLVVDSSQNNFERGFLENGQTREHAYLLRALGI 315
Score = 49.6 bits (113), Expect = 4e-07
Identities = 21/47 (44%), Positives = 32/47 (68%)
Frame = +3
Query: 117 KTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGK 257
K +++VV GHVDSGKST G ++++ G I+ R+++K EA GK
Sbjct: 175 KPVVHLVVTGHVDSGKSTMLGRIMFELGEINSRSMQKLHNEAANSGK 221
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 90.2 bits (214), Expect = 2e-19
Identities = 43/96 (44%), Positives = 59/96 (61%)
Frame = +2
Query: 221 REVREGGPGNG*GSFKYAWVLDKLKAERELGITIDIALWKFETSKYYVTIIDAPGHRDFI 400
RE +E G S+ +W LD ERE G T+++ FET +++DAPGH+ ++
Sbjct: 275 REAKEAGKE----SWYLSWALDSTSEEREKGKTVEVGRAYFETEHRRFSLLDAPGHKGYV 330
Query: 401 KNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTRE 508
NMI G SQAD VL+++A GEFEAG + GQTRE
Sbjct: 331 TNMINGASQADIGVLVISARRGEFEAGFERGGQTRE 366
Score = 67.3 bits (157), Expect = 2e-12
Identities = 28/48 (58%), Positives = 39/48 (81%)
Frame = +3
Query: 117 KTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKD 260
K H+NIV IGHVD+GKST G++++ G +DKRT+EK E+EA+E GK+
Sbjct: 236 KEHVNIVFIGHVDAGKSTLGGNILFLTGMVDKRTMEKIEREAKEAGKE 283
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 61.7 bits (143), Expect = 1e-10
Identities = 37/97 (38%), Positives = 52/97 (53%)
Frame = +2
Query: 251 G*GSFKYAWVLDKLKAERELGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQA 430
G SF +DK E+ GITI A ++ET+ + +D PGH D+IKNMITG +
Sbjct: 81 GQASFMDYSQIDKAPEEKARGITISSAHVEYETANRHYAHVDCPGHADYIKNMITGAATM 140
Query: 431 DCAVLIVAAGTGEFEAGISKNGQTREMPCSLSPLGVK 541
D A+++V+A G+ QTRE +GVK
Sbjct: 141 DGAIIVVSATDGQMP-------QTREHLLLARQVGVK 170
Score = 35.1 bits (77), Expect = 0.009
Identities = 16/29 (55%), Positives = 20/29 (68%)
Frame = +3
Query: 111 KEKTHINIVVIGHVDSGKSTTTGHLIYKC 197
++K H+NI IGHVD GK+T T I KC
Sbjct: 49 RKKPHVNIGTIGHVDHGKTTLTA-AITKC 76
>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 813
Score = 45.6 bits (103), Expect = 7e-06
Identities = 24/63 (38%), Positives = 32/63 (50%)
Frame = +2
Query: 278 VLDKLKAERELGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAA 457
V+D L AER+ GITI+ A F + +ID PGH DF + + D AV I+
Sbjct: 67 VMDYLPAERQRGITINSAAISFTWRNQRINLIDTPGHADFTFEVERSVAVLDGAVAIIDG 126
Query: 458 GTG 466
G
Sbjct: 127 SAG 129
Score = 28.7 bits (61), Expect = 0.82
Identities = 10/24 (41%), Positives = 17/24 (70%)
Frame = +3
Query: 129 NIVVIGHVDSGKSTTTGHLIYKCG 200
N+ +I H+D+GK+T T ++Y G
Sbjct: 30 NVGIIAHIDAGKTTLTEKMLYYGG 53
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 41.1 bits (92), Expect = 1e-04
Identities = 21/66 (31%), Positives = 34/66 (51%), Gaps = 4/66 (6%)
Frame = +2
Query: 281 LDKLKAERELGITIDIA----LWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 448
LDKL+ ER GIT+ ++ + Y + +ID PGH DF ++ + + +L+
Sbjct: 95 LDKLEVERRRGITVKAQTCSMIYYYHGQSYLLNLIDTPGHVDFRAEVMHSLAACEGCILL 154
Query: 449 VAAGTG 466
V A G
Sbjct: 155 VDASQG 160
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 35.5 bits (78), Expect = 0.007
Identities = 17/32 (53%), Positives = 19/32 (59%)
Frame = +3
Query: 105 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCG 200
MGK N+ VI HVD GKST T L+ K G
Sbjct: 13 MGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAG 44
Score = 27.9 bits (59), Expect = 1.4
Identities = 11/32 (34%), Positives = 18/32 (56%)
Frame = +2
Query: 356 YYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 451
+ V +ID+PGH DF + D A+++V
Sbjct: 98 FLVNLIDSPGHVDFSSEVTAALRVTDGALVVV 129
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 35.5 bits (78), Expect = 0.007
Identities = 17/32 (53%), Positives = 19/32 (59%)
Frame = +3
Query: 105 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCG 200
MGK N+ VI HVD GKST T L+ K G
Sbjct: 13 MGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAG 44
Score = 27.9 bits (59), Expect = 1.4
Identities = 11/32 (34%), Positives = 18/32 (56%)
Frame = +2
Query: 356 YYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 451
+ V +ID+PGH DF + D A+++V
Sbjct: 98 FLVNLIDSPGHVDFSSEVTAALRVTDGALVVV 129
>SPBC1271.15c |||translation initiation factor
IF-2Mt|Schizosaccharomyces pombe|chr 2|||Manual
Length = 686
Score = 33.9 bits (74), Expect = 0.022
Identities = 19/54 (35%), Positives = 26/54 (48%), Gaps = 2/54 (3%)
Frame = +2
Query: 311 GITIDIALWK--FETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 466
GIT I + F+ ++T +D PGH F G + AD VL+VA G
Sbjct: 203 GITQKIGAFTVPFDKGSKFITFLDTPGHMAFEAMRKRGANIADIVVLVVAGDDG 256
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 33.5 bits (73), Expect = 0.029
Identities = 21/55 (38%), Positives = 28/55 (50%)
Frame = +2
Query: 341 FETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTR 505
FE S Y + IID PGH DF + D AVL++ A +G I+ + Q R
Sbjct: 141 FEKS-YNINIIDTPGHIDFTIEVERALRVLDGAVLVLCAVSGVQSQTITVDRQMR 194
Score = 29.1 bits (62), Expect = 0.62
Identities = 12/26 (46%), Positives = 17/26 (65%)
Frame = +3
Query: 129 NIVVIGHVDSGKSTTTGHLIYKCGGI 206
NI + H+DSGK+T T ++Y G I
Sbjct: 61 NIGISAHIDSGKTTFTERVLYYTGRI 86
>SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 446
Score = 31.1 bits (67), Expect = 0.15
Identities = 11/32 (34%), Positives = 20/32 (62%)
Frame = +2
Query: 359 YVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 454
+V+ +D PGH + M+ G + D A+L++A
Sbjct: 109 HVSFVDCPGHDILMATMLNGAAVMDAALLLIA 140
>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 1000
Score = 30.3 bits (65), Expect = 0.27
Identities = 13/37 (35%), Positives = 21/37 (56%)
Frame = +2
Query: 356 YYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 466
Y + +ID+PGH DF + + + D A ++V A G
Sbjct: 94 YLINLIDSPGHVDFSSEVSSASRLCDGAFVLVDAVEG 130
>SPAC56F8.03 |||translation initiation factor IF2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1079
Score = 29.5 bits (63), Expect = 0.47
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +2
Query: 368 IIDAPGHRDFIKNMITGTSQADCAVLIV 451
IID PGH F GTS + A+L++
Sbjct: 553 IIDTPGHESFTNLRSRGTSLCNIAILVI 580
>SPAC328.01c ||SPAC3A11.01|karyopherin|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1234
Score = 29.1 bits (62), Expect = 0.62
Identities = 23/78 (29%), Positives = 39/78 (50%), Gaps = 3/78 (3%)
Frame = -3
Query: 451 YDEHSAISLRGSCDHVLDEISVSRSINDGNIVLASFELPE---SNIDCDTELTLSL*FVQ 281
+ S+ R S +LDE+ S S ++ + EL E S++ C ++ + F
Sbjct: 17 HSPESSRETRFSAQQLLDELKDSYS--SPSVAIQLLELNEQAFSSLGCKLDIHIVQHFSL 74
Query: 280 YPSIFEGSLPISWASFSN 227
S+FE S+ ++W SFSN
Sbjct: 75 --SLFETSVGMNWKSFSN 90
>SPAC9G1.11c |spn4||septin Spn4|Schizosaccharomyces pombe|chr
1|||Manual
Length = 380
Score = 28.3 bits (60), Expect = 1.1
Identities = 15/44 (34%), Positives = 26/44 (59%), Gaps = 3/44 (6%)
Frame = +2
Query: 284 DKLKAER-ELGITIDIALWKFETSKYYV--TIIDAPGHRDFIKN 406
+K++A+ E + I+I + E +++ T+ID PG DFI N
Sbjct: 60 EKVRAKHAEKTVEIEITKAELEEKNFHLRLTVIDTPGFGDFINN 103
>SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 983
Score = 27.5 bits (58), Expect = 1.9
Identities = 16/57 (28%), Positives = 28/57 (49%), Gaps = 3/57 (5%)
Frame = +2
Query: 290 LKAERELGIT---IDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 451
L+ ER + I + +A+ + + ID PGH DF+ + + +D VL+V
Sbjct: 184 LERERVMSIKSTPLTLAVSDMKGKTFAFQCIDTPGHVDFVDEVAAPMAISDGVVLVV 240
>SPAC23E2.03c |ste7||meiotic suppressor protein
Ste7|Schizosaccharomyces pombe|chr 1|||Manual
Length = 569
Score = 25.8 bits (54), Expect = 5.8
Identities = 14/35 (40%), Positives = 17/35 (48%)
Frame = -1
Query: 312 PSSRSAFSLSNTQAYLKDPYPFPGPPSRTSRWYVC 208
PS SAF N Q+YL + P S +SR C
Sbjct: 278 PSQFSAFMYQNQQSYLSPQSHYENPLSISSRPSPC 312
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,937,911
Number of Sequences: 5004
Number of extensions: 61633
Number of successful extensions: 220
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 196
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 219
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 313902888
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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