Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30503
(681 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 181 6e-48
X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 177 8e-47
AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 167 9e-44
EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 137 1e-34
AF069739-1|AAC63272.2| 690|Apis mellifera translation initiatio... 33 0.003
AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methylt... 24 1.2
DQ257415-1|ABB81846.1| 430|Apis mellifera yellow-like protein p... 23 2.0
AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase pro... 23 2.7
D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein. 23 3.6
>AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha
F2 protein.
Length = 461
Score = 181 bits (440), Expect = 6e-48
Identities = 88/97 (90%), Positives = 88/97 (90%)
Frame = +2
Query: 251 G*GSFKYAWVLDKLKAERELGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQA 430
G GSFKYAWVLDKLKAERE GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQA
Sbjct: 50 GKGSFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQA 109
Query: 431 DCAVLIVAAGTGEFEAGISKNGQTREMPCSLSPLGVK 541
DCAVLIVAAGTGEFEAGISKNGQTRE LGVK
Sbjct: 110 DCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVK 146
Score = 109 bits (262), Expect = 2e-26
Identities = 50/51 (98%), Positives = 50/51 (98%)
Frame = +3
Query: 105 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGK 257
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGK
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGK 51
Score = 34.3 bits (75), Expect = 0.001
Identities = 19/47 (40%), Positives = 29/47 (61%)
Frame = +3
Query: 540 KQLIVGVNQMGSTEPTIQ*AQIFEGKFKKGRLSLITSKERLGYKPQA 680
KQLIVGVN+M STEP E +F++ + + + +++GY P A
Sbjct: 146 KQLIVGVNKMDSTEPPYS-----ETRFEEIKKEVSSYIKKIGYNPAA 187
>X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha
protein.
Length = 461
Score = 177 bits (431), Expect = 8e-47
Identities = 86/97 (88%), Positives = 87/97 (89%)
Frame = +2
Query: 251 G*GSFKYAWVLDKLKAERELGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQA 430
G GSFKYAWVLDKLKAERE GITIDIALWKFET+KYYVTIIDAPGHRDFIKNMITGTSQA
Sbjct: 50 GKGSFKYAWVLDKLKAERERGITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQA 109
Query: 431 DCAVLIVAAGTGEFEAGISKNGQTREMPCSLSPLGVK 541
DCAVLIVAAG GEFEAGISKNGQTRE LGVK
Sbjct: 110 DCAVLIVAAGIGEFEAGISKNGQTREHALLAFTLGVK 146
Score = 109 bits (262), Expect = 2e-26
Identities = 50/51 (98%), Positives = 50/51 (98%)
Frame = +3
Query: 105 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGK 257
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGK
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGK 51
Score = 27.9 bits (59), Expect = 0.095
Identities = 15/43 (34%), Positives = 26/43 (60%)
Frame = +3
Query: 540 KQLIVGVNQMGSTEPTIQ*AQIFEGKFKKGRLSLITSKERLGY 668
KQLIVGVN+M T+P E +F++ + + + +++GY
Sbjct: 146 KQLIVGVNKMDMTDPPYS-----EARFEEIKKEVSSYIKKIGY 183
>AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor
1-alpha protein.
Length = 274
Score = 167 bits (406), Expect = 9e-44
Identities = 81/89 (91%), Positives = 81/89 (91%)
Frame = +2
Query: 275 WVLDKLKAERELGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 454
WVLDKLKAERE GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA
Sbjct: 1 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 60
Query: 455 AGTGEFEAGISKNGQTREMPCSLSPLGVK 541
AGTGEFEAGISKNGQTRE LGVK
Sbjct: 61 AGTGEFEAGISKNGQTREHALLAFTLGVK 89
Score = 34.3 bits (75), Expect = 0.001
Identities = 19/47 (40%), Positives = 29/47 (61%)
Frame = +3
Query: 540 KQLIVGVNQMGSTEPTIQ*AQIFEGKFKKGRLSLITSKERLGYKPQA 680
KQLIVGVN+M STEP E +F++ + + + +++GY P A
Sbjct: 89 KQLIVGVNKMDSTEPPYS-----ETRFEEIKKEVSSYIKKIGYNPAA 130
>EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor
1-alpha protein.
Length = 172
Score = 137 bits (331), Expect = 1e-34
Identities = 66/73 (90%), Positives = 66/73 (90%)
Frame = +2
Query: 323 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 502
DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT
Sbjct: 1 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 60
Query: 503 REMPCSLSPLGVK 541
RE LGVK
Sbjct: 61 REHALLAFTLGVK 73
Score = 34.3 bits (75), Expect = 0.001
Identities = 19/47 (40%), Positives = 29/47 (61%)
Frame = +3
Query: 540 KQLIVGVNQMGSTEPTIQ*AQIFEGKFKKGRLSLITSKERLGYKPQA 680
KQLIVGVN+M STEP E +F++ + + + +++GY P A
Sbjct: 73 KQLIVGVNKMDSTEPPYS-----ETRFEEIKKEVSSYIKKIGYNPAA 114
>AF069739-1|AAC63272.2| 690|Apis mellifera translation initiation
factor 2 protein.
Length = 690
Score = 33.1 bits (72), Expect = 0.003
Identities = 16/35 (45%), Positives = 18/35 (51%)
Frame = +2
Query: 362 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 466
VT +D PGH FI G D VL+VAA G
Sbjct: 195 VTFLDTPGHAAFISMRHRGAHITDIVVLVVAADDG 229
Score = 25.4 bits (53), Expect = 0.51
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = +3
Query: 117 KTHINIVVIGHVDSGKST 170
K H + ++GHVD GK+T
Sbjct: 143 KRHPIVTIMGHVDHGKTT 160
>AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9
methyltransferase protein.
Length = 683
Score = 24.2 bits (50), Expect = 1.2
Identities = 11/15 (73%), Positives = 11/15 (73%)
Frame = +3
Query: 126 INIVVIGHVDSGKST 170
INI IGHV GKST
Sbjct: 43 INIGTIGHVAHGKST 57
>DQ257415-1|ABB81846.1| 430|Apis mellifera yellow-like protein
protein.
Length = 430
Score = 23.4 bits (48), Expect = 2.0
Identities = 8/19 (42%), Positives = 12/19 (63%)
Frame = +1
Query: 616 NSRKGGYPSLHPRKDWATN 672
++ +GG P L P +WA N
Sbjct: 100 DTNRGGSPKLTPYPNWAQN 118
>AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase
protein.
Length = 567
Score = 23.0 bits (47), Expect = 2.7
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = +2
Query: 347 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 448
T KYY D P + FIKN+ ++ +D LI
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLI 327
>D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein.
Length = 567
Score = 22.6 bits (46), Expect = 3.6
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = +2
Query: 347 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 448
T KYY D P + FIKN+ ++ +D L+
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLV 327
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 202,506
Number of Sequences: 438
Number of extensions: 4029
Number of successful extensions: 21
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 13
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 20
length of database: 146,343
effective HSP length: 56
effective length of database: 121,815
effective search space used: 20708550
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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