Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30501
(501 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_P46776 Cluster: 60S ribosomal protein L27a; n=204; Euka... 93 4e-18
UniRef50_Q9LR33 Cluster: 60S ribosomal protein L27a-2; n=56; Euk... 90 2e-17
UniRef50_Q00V89 Cluster: 60s ribosomal protein L15/L27; n=2; Ost... 83 3e-15
UniRef50_A1BQ56 Cluster: Ribosomal protein L27a; n=4; Bilateria|... 82 8e-15
UniRef50_Q00454 Cluster: 60S ribosomal protein L27a; n=3; Eukary... 81 1e-14
UniRef50_Q9AW02 Cluster: 60S ribosomal protein L27A; n=3; Eukary... 62 9e-09
UniRef50_Q4P992 Cluster: Putative uncharacterized protein; n=1; ... 52 6e-06
UniRef50_O62581 Cluster: 60S ribosomal protein L27a; n=1; Enceph... 52 6e-06
UniRef50_Q3LW97 Cluster: Ribosomal protein L27a; n=1; Bigelowiel... 51 2e-05
UniRef50_A7QSF4 Cluster: Chromosome chr12 scaffold_159, whole ge... 43 0.004
UniRef50_UPI0000DA3FAD Cluster: PREDICTED: similar to 60S riboso... 39 0.073
UniRef50_A4ANL3 Cluster: Putative lipoprotein; n=1; Flavobacteri... 34 2.1
UniRef50_Q9ZQM0 Cluster: Putative non-LTR retroelement reverse t... 33 4.8
UniRef50_A7RPS0 Cluster: Predicted protein; n=1; Nematostella ve... 32 8.3
>UniRef50_P46776 Cluster: 60S ribosomal protein L27a; n=204;
Eukaryota|Rep: 60S ribosomal protein L27a - Homo sapiens
(Human)
Length = 148
Score = 92.7 bits (220), Expect = 4e-18
Identities = 38/73 (52%), Positives = 49/73 (67%)
Frame = +1
Query: 37 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGYFGKLGMRN 216
M + +KTRKLRGHVS NAGG HHHRIN DKYHPGYFGK+GM++
Sbjct: 1 MPSRLRKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGLHHHRINFDKYHPGYFGKVGMKH 60
Query: 217 FHFRKNKNFCPVL 255
+H ++N++FCP +
Sbjct: 61 YHLKRNQSFCPTV 73
Score = 70.1 bits (164), Expect = 3e-11
Identities = 37/88 (42%), Positives = 46/88 (52%)
Frame = +3
Query: 216 FPL*KEQEFLSSFNLDKLWTLVSEQTRLKYASAPDGKVPVINIVKAXXXXXXXXXXXPKQ 395
+ L + Q F + NLDKLWTLVSEQTR+ A G P+I++V++ PKQ
Sbjct: 61 YHLKRNQSFCPTVNLDKLWTLVSEQTRVNAAKNKTGAAPIIDVVRSGYYKVLGKGKLPKQ 120
Query: 396 PVIVXXXXXXXXXXXXXXDVGGACVLSA 479
PVIV VGGACVL A
Sbjct: 121 PVIVKAKFFSRRAEEKIKSVGGACVLVA 148
>UniRef50_Q9LR33 Cluster: 60S ribosomal protein L27a-2; n=56;
Eukaryota|Rep: 60S ribosomal protein L27a-2 -
Arabidopsis thaliana (Mouse-ear cress)
Length = 146
Score = 90.2 bits (214), Expect = 2e-17
Identities = 41/73 (56%), Positives = 46/73 (63%)
Frame = +1
Query: 37 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGYFGKLGMRN 216
MAT+ KK RK RGHVS NAGG HHHRI DKYHPGYFGK+GMR
Sbjct: 1 MATALKKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRY 60
Query: 217 FHFRKNKNFCPVL 255
FH +NK FCP++
Sbjct: 61 FHKLRNKFFCPIV 73
Score = 35.1 bits (77), Expect = 0.90
Identities = 25/81 (30%), Positives = 37/81 (45%), Gaps = 1/81 (1%)
Frame = +3
Query: 240 FLSSFNLDKLWTLVSEQTRLKYASAPDGKVPVINIVKAXXXXXXXXXXXPK-QPVIVXXX 416
F NLDKLW+LV E + K S+ D VP+I++ + P+ +P +V
Sbjct: 69 FCPIVNLDKLWSLVPEDVKAK--SSKD-NVPLIDVTQHGFFKVLGKGHLPENKPFVVKAK 125
Query: 417 XXXXXXXXXXXDVGGACVLSA 479
+ GGA VL+A
Sbjct: 126 LISKTAEKKIKEAGGAVVLTA 146
>UniRef50_Q00V89 Cluster: 60s ribosomal protein L15/L27; n=2;
Ostreococcus|Rep: 60s ribosomal protein L15/L27 -
Ostreococcus tauri
Length = 198
Score = 83.0 bits (196), Expect = 3e-15
Identities = 37/71 (52%), Positives = 42/71 (59%)
Frame = +1
Query: 43 TSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGYFGKLGMRNFH 222
T K RK RGHVS AGG+HHHRI MDKYHPGYFGK+GMR+FH
Sbjct: 54 TQLHKNRKKRGHVSAGHGRIGKHRKHPAGRGMAGGQHHHRIMMDKYHPGYFGKVGMRHFH 113
Query: 223 FRKNKNFCPVL 255
KN+ CP +
Sbjct: 114 KLKNQTVCPTI 124
Score = 48.0 bits (109), Expect = 1e-04
Identities = 27/84 (32%), Positives = 38/84 (45%)
Frame = +3
Query: 228 KEQEFLSSFNLDKLWTLVSEQTRLKYASAPDGKVPVINIVKAXXXXXXXXXXXPKQPVIV 407
K Q + N+DKLWTLV + R + A + PVI++ P QPV+V
Sbjct: 116 KNQTVCPTINIDKLWTLVGTEKREECAKNTS-QAPVIDLTDHGIFKLLGKGQLPNQPVVV 174
Query: 408 XXXXXXXXXXXXXXDVGGACVLSA 479
+VGGAC+L+A
Sbjct: 175 KCRYISKLAEKKIKEVGGACILTA 198
>UniRef50_A1BQ56 Cluster: Ribosomal protein L27a; n=4;
Bilateria|Rep: Ribosomal protein L27a - Ascaris suum
(Pig roundworm) (Ascaris lumbricoides)
Length = 133
Score = 81.8 bits (193), Expect = 8e-15
Identities = 31/40 (77%), Positives = 35/40 (87%)
Frame = +1
Query: 136 NAGGEHHHRINMDKYHPGYFGKLGMRNFHFRKNKNFCPVL 255
NAGG+HHHRIN DKYHPGYFGK+GMRNFH RKN +CP +
Sbjct: 20 NAGGQHHHRINFDKYHPGYFGKVGMRNFHVRKNGLYCPTV 59
Score = 66.1 bits (154), Expect = 4e-10
Identities = 38/88 (43%), Positives = 46/88 (52%)
Frame = +3
Query: 216 FPL*KEQEFLSSFNLDKLWTLVSEQTRLKYASAPDGKVPVINIVKAXXXXXXXXXXXPKQ 395
F + K + + NLDKLW+LVSE+TR YA D K PVI++VKA PKQ
Sbjct: 47 FHVRKNGLYCPTVNLDKLWSLVSEKTRTTYAEKKD-KAPVIDVVKAGYFKVLGKGLLPKQ 105
Query: 396 PVIVXXXXXXXXXXXXXXDVGGACVLSA 479
PVIV VGGAC+L A
Sbjct: 106 PVIVKAKFFSRTAEQKIKGVGGACILVA 133
>UniRef50_Q00454 Cluster: 60S ribosomal protein L27a; n=3;
Eukaryota|Rep: 60S ribosomal protein L27a - Tetrahymena
thermophila
Length = 149
Score = 81.4 bits (192), Expect = 1e-14
Identities = 39/75 (52%), Positives = 45/75 (60%), Gaps = 2/75 (2%)
Frame = +1
Query: 37 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXN--AGGEHHHRINMDKYHPGYFGKLGM 210
M + KKTRKLRGHVS AGG HHHRI M+K+HPGY+GKLGM
Sbjct: 1 MVSHLKKTRKLRGHVSHGHGRVGKHRKGGCRGGRGKAGGMHHHRILMEKWHPGYYGKLGM 60
Query: 211 RNFHFRKNKNFCPVL 255
R FH +KN CPV+
Sbjct: 61 RTFHLKKNPLHCPVV 75
Score = 60.1 bits (139), Expect = 3e-08
Identities = 33/75 (44%), Positives = 40/75 (53%)
Frame = +3
Query: 255 NLDKLWTLVSEQTRLKYASAPDGKVPVINIVKAXXXXXXXXXXXPKQPVIVXXXXXXXXX 434
N+DKLW+LVS+ TR KYA KVPVI++ KA P QPV+V
Sbjct: 76 NIDKLWSLVSDATRQKYAE-DKKKVPVIDVTKAGFFKVLGKGRLPNQPVVVKAKYFSKTA 134
Query: 435 XXXXXDVGGACVLSA 479
VGGACVL+A
Sbjct: 135 ERRIVAVGGACVLTA 149
>UniRef50_Q9AW02 Cluster: 60S ribosomal protein L27A; n=3;
Eukaryota|Rep: 60S ribosomal protein L27A - Guillardia
theta (Cryptomonas phi)
Length = 144
Score = 61.7 bits (143), Expect = 9e-09
Identities = 28/69 (40%), Positives = 37/69 (53%)
Frame = +1
Query: 37 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGYFGKLGMRN 216
M T +++RK RGHVS N+GG+HHH+I +KYH +FGK GMR
Sbjct: 1 MPTDIRRSRKKRGHVSSGHGRVGKHRKHPGGRGNSGGQHHHKIFFEKYHKKHFGKTGMRY 60
Query: 217 FHFRKNKNF 243
+ K NF
Sbjct: 61 YRKLKQNNF 69
>UniRef50_Q4P992 Cluster: Putative uncharacterized protein; n=1;
Ustilago maydis|Rep: Putative uncharacterized protein -
Ustilago maydis (Smut fungus)
Length = 187
Score = 52.4 bits (120), Expect = 6e-06
Identities = 25/50 (50%), Positives = 26/50 (52%)
Frame = +1
Query: 37 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHP 186
M T KTRK RGHVS AGG+HHHR NMDKYHP
Sbjct: 1 MPTHLSKTRKHRGHVSAGNGRVGKHRKHPGGRGLAGGQHHHRTNMDKYHP 50
Score = 38.3 bits (85), Expect = 0.096
Identities = 28/88 (31%), Positives = 35/88 (39%)
Frame = +3
Query: 216 FPL*KEQEFLSSFNLDKLWTLVSEQTRLKYASAPDGKVPVINIVKAXXXXXXXXXXXPKQ 395
F L + F NLDKLWTLV + R K +A +VPVI+ + A P
Sbjct: 101 FHLTRNSSFRPVVNLDKLWTLVPAEQR-KGLTADSTEVPVIDTLAAGYGKVLGKGRLPAL 159
Query: 396 PVIVXXXXXXXXXXXXXXDVGGACVLSA 479
P IV + GG L A
Sbjct: 160 PCIVKARWVSSLAEKKIKEAGGVVKLVA 187
Score = 36.7 bits (81), Expect = 0.29
Identities = 14/37 (37%), Positives = 22/37 (59%)
Frame = +1
Query: 145 GEHHHRINMDKYHPGYFGKLGMRNFHFRKNKNFCPVL 255
G ++ + YFGK+GMR+FH +N +F PV+
Sbjct: 77 GSQEMEVDREIGRDSYFGKVGMRHFHLTRNSSFRPVV 113
>UniRef50_O62581 Cluster: 60S ribosomal protein L27a; n=1;
Encephalitozoon cuniculi|Rep: 60S ribosomal protein L27a
- Encephalitozoon cuniculi
Length = 147
Score = 52.4 bits (120), Expect = 6e-06
Identities = 26/69 (37%), Positives = 34/69 (49%)
Frame = +1
Query: 37 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGYFGKLGMRN 216
M KKTRKLRGHVS AGG H + ++HP Y GK GMR
Sbjct: 1 MTDRVKKTRKLRGHVSHGYGRVGKHRKHSGGRGLAGGFSHMKTFFTRFHPDYHGKRGMRV 60
Query: 217 FHFRKNKNF 243
+H ++N ++
Sbjct: 61 YHRKENSDY 69
>UniRef50_Q3LW97 Cluster: Ribosomal protein L27a; n=1; Bigelowiella
natans|Rep: Ribosomal protein L27a - Bigelowiella natans
(Pedinomonas minutissima) (Chlorarachnion sp.(strain
CCMP 621))
Length = 151
Score = 50.8 bits (116), Expect = 2e-05
Identities = 24/68 (35%), Positives = 33/68 (48%)
Frame = +1
Query: 37 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGYFGKLGMRN 216
M T KK+RK+RG S +GG+HHH+I ++Y YF K GMR
Sbjct: 1 MVTKLKKSRKMRGKNSSGHGRINKHRKNPGGKGKSGGQHHHKIIFERYRETYFRKTGMRI 60
Query: 217 FHFRKNKN 240
++ K N
Sbjct: 61 YNLEKTLN 68
>UniRef50_A7QSF4 Cluster: Chromosome chr12 scaffold_159, whole
genome shotgun sequence; n=3; Vitis vinifera|Rep:
Chromosome chr12 scaffold_159, whole genome shotgun
sequence - Vitis vinifera (Grape)
Length = 151
Score = 42.7 bits (96), Expect = 0.004
Identities = 16/28 (57%), Positives = 22/28 (78%)
Frame = +1
Query: 172 DKYHPGYFGKLGMRNFHFRKNKNFCPVL 255
DK+HPG FGK+GMR+FH + K CP++
Sbjct: 28 DKHHPGNFGKVGMRHFHKLRIKLHCPIV 55
>UniRef50_UPI0000DA3FAD Cluster: PREDICTED: similar to 60S ribosomal
protein L27a; n=1; Rattus norvegicus|Rep: PREDICTED:
similar to 60S ribosomal protein L27a - Rattus
norvegicus
Length = 86
Score = 38.7 bits (86), Expect = 0.073
Identities = 21/51 (41%), Positives = 24/51 (47%)
Frame = +1
Query: 37 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPG 189
M+ +KTRK +GHVS NAGGEH HRIN PG
Sbjct: 1 MSGRLRKTRKFQGHVSDSHSRISKHHKHRGGQRNAGGEHLHRINCYYKVPG 51
>UniRef50_A4ANL3 Cluster: Putative lipoprotein; n=1;
Flavobacteriales bacterium HTCC2170|Rep: Putative
lipoprotein - Flavobacteriales bacterium HTCC2170
Length = 535
Score = 33.9 bits (74), Expect = 2.1
Identities = 16/32 (50%), Positives = 19/32 (59%)
Frame = -3
Query: 469 TQAPPTSLIFFSADFEKNFAFTMTGCLGSLPL 374
T A SL FFS D + N A T GCLG+ P+
Sbjct: 306 TDAEGNSLYFFSLDTKDNSACTSAGCLGAWPV 337
>UniRef50_Q9ZQM0 Cluster: Putative non-LTR retroelement reverse
transcriptase; n=1; Arabidopsis thaliana|Rep: Putative
non-LTR retroelement reverse transcriptase - Arabidopsis
thaliana (Mouse-ear cress)
Length = 1044
Score = 32.7 bits (71), Expect = 4.8
Identities = 14/30 (46%), Positives = 19/30 (63%)
Frame = +3
Query: 171 GQVPSWILWQTWYEKFPL*KEQEFLSSFNL 260
GQ+P WILW W + L EQ+ +SS +L
Sbjct: 842 GQLPIWILWNLWNCRNKLIFEQKHISSMDL 871
>UniRef50_A7RPS0 Cluster: Predicted protein; n=1; Nematostella
vectensis|Rep: Predicted protein - Nematostella
vectensis
Length = 306
Score = 31.9 bits (69), Expect = 8.3
Identities = 17/46 (36%), Positives = 23/46 (50%)
Frame = -3
Query: 244 RNSCSF*SGNFSYQVCQSIQDGTCPC*FCDGAHHQHYHDLLDAYGA 107
R+SC + F +C+S T PC D H +H DL+D GA
Sbjct: 108 RSSCQ--ARCFMNNLCRSYNYNTTPCQLSDSDHLEHPSDLVDKPGA 151
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 466,316,457
Number of Sequences: 1657284
Number of extensions: 8684119
Number of successful extensions: 20965
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 20225
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 20944
length of database: 575,637,011
effective HSP length: 95
effective length of database: 418,195,031
effective search space used: 29691847201
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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