BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30501 (501 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC776.11 |rpl2801|rpl28-1|60S ribosomal protein Ll28B 27a|Schi... 72 4e-14 SPCC5E4.07 |rpl2802|rpl28-2|60S ribosomal protein L27a.2/L28A|Sc... 72 6e-14 SPBC29A10.08 |||1,3-beta-glucanosyltransferase |Schizosaccharomy... 25 8.4 SPBC1921.02 |rad60||DNA repair protein Rad60 |Schizosaccharomyce... 25 8.4 >SPBC776.11 |rpl2801|rpl28-1|60S ribosomal protein Ll28B 27a|Schizosaccharomyces pombe|chr 2|||Manual Length = 148 Score = 72.1 bits (169), Expect = 4e-14 Identities = 35/73 (47%), Positives = 39/73 (53%) Frame = +1 Query: 37 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGYFGKLGMRN 216 M T KTRKLRGHVS AGG H R + DKYHPGYFGK+GMR Sbjct: 1 MPTHTSKTRKLRGHVSAGHGRIGKHRKHPGGRGKAGGLQHLRSHFDKYHPGYFGKVGMRR 60 Query: 217 FHFRKNKNFCPVL 255 FH KN + P + Sbjct: 61 FHLMKNPLWRPTV 73 Score = 45.2 bits (102), Expect = 6e-06 Identities = 27/89 (30%), Positives = 37/89 (41%) Frame = +3 Query: 213 KFPL*KEQEFLSSFNLDKLWTLVSEQTRLKYASAPDGKVPVINIVKAXXXXXXXXXXXPK 392 +F L K + + NLD+LWTLV +TR KY PVIN++++ P Sbjct: 60 RFHLMKNPLWRPTVNLDRLWTLVPNETREKYLGKNTEVAPVINVLQSGYGKVLGKGRLPD 119 Query: 393 QPVIVXXXXXXXXXXXXXXDVGGACVLSA 479 PVI+ GG L A Sbjct: 120 TPVIIQTRYVSRRAEEKIKQAGGVVELIA 148 >SPCC5E4.07 |rpl2802|rpl28-2|60S ribosomal protein L27a.2/L28A|Schizosaccharomyces pombe|chr 3|||Manual Length = 148 Score = 71.7 bits (168), Expect = 6e-14 Identities = 35/73 (47%), Positives = 39/73 (53%) Frame = +1 Query: 37 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGYFGKLGMRN 216 M T KTRKLRGHVS AGG H R + DKYHPGYFGK+GMR Sbjct: 1 MPTHVSKTRKLRGHVSAGHGRIGKHRKHPGGRGKAGGLQHLRSHFDKYHPGYFGKVGMRR 60 Query: 217 FHFRKNKNFCPVL 255 FH KN + P + Sbjct: 61 FHLMKNPLWRPTV 73 Score = 42.7 bits (96), Expect = 3e-05 Identities = 26/89 (29%), Positives = 37/89 (41%) Frame = +3 Query: 213 KFPL*KEQEFLSSFNLDKLWTLVSEQTRLKYASAPDGKVPVINIVKAXXXXXXXXXXXPK 392 +F L K + + NLD+LWTL+ + R KY PVIN++++ P+ Sbjct: 60 RFHLMKNPLWRPTVNLDRLWTLLPNEARDKYLGKNTEVAPVINVLQSGYGKVLGKGRLPE 119 Query: 393 QPVIVXXXXXXXXXXXXXXDVGGACVLSA 479 PVIV GG L A Sbjct: 120 TPVIVQTRYVSRRAEEKIKQAGGVVELIA 148 >SPBC29A10.08 |||1,3-beta-glucanosyltransferase |Schizosaccharomyces pombe|chr 2|||Manual Length = 459 Score = 24.6 bits (51), Expect = 8.4 Identities = 9/26 (34%), Positives = 14/26 (53%) Frame = +3 Query: 255 NLDKLWTLVSEQTRLKYASAPDGKVP 332 NL K W +S K++ P+G +P Sbjct: 323 NLKKQWASISPNITYKHSYNPNGTIP 348 >SPBC1921.02 |rad60||DNA repair protein Rad60 |Schizosaccharomyces pombe|chr 2|||Manual Length = 406 Score = 24.6 bits (51), Expect = 8.4 Identities = 10/20 (50%), Positives = 15/20 (75%) Frame = -2 Query: 347 DNIDDGDLAIWSRCILQPRL 288 DN+D+ DLA +S+ I +P L Sbjct: 2 DNLDEDDLAFFSKPIKKPPL 21 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,930,336 Number of Sequences: 5004 Number of extensions: 36835 Number of successful extensions: 80 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 76 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 78 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 198176188 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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