BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30498 (708 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 182 4e-47 SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 177 1e-45 SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 78 1e-15 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 62 1e-10 SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom... 34 0.023 SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 31 0.21 SPCC18.03 |||shuttle craft like transcriptional regulator|Schizo... 30 0.28 SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 30 0.28 SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1||... 28 1.1 SPBC428.07 |meu6||meiotic chromosome segregation protein Meu6|Sc... 28 1.1 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 28 1.1 SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|ch... 26 4.6 SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch... 26 6.1 SPCC320.07c |mde7||RNA-binding protein Mde7|Schizosaccharomyces ... 26 6.1 SPAC6G9.04 |mug79||meiotically upregulated gene Mug79|Schizosacc... 25 8.0 SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces po... 25 8.0 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 182 bits (443), Expect = 4e-47 Identities = 79/109 (72%), Positives = 91/109 (83%) Frame = +2 Query: 245 EGLHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKR 424 + HE SV EITN CFEP NQMVKCDPR G+YMA C+LYRGDV+P+DV AA+ +IK++R Sbjct: 284 KAFHESNSVQEITNQCFEPYNQMVKCDPRTGRYMATCLLYRGDVIPRDVQAAVTSIKSRR 343 Query: 425 TIQFVDWCPTGFKVGINYQPPTVVPGGDWAKVQRAVCMLSNTTAIAEAW 571 TIQFVDWCPTGFK+GI Y+PP VPG AKV RAVCMLSNTT+IAEAW Sbjct: 344 TIQFVDWCPTGFKIGICYEPPQHVPGSGIAKVNRAVCMLSNTTSIAEAW 392 Score = 170 bits (413), Expect = 2e-43 Identities = 100/221 (45%), Positives = 121/221 (54%), Gaps = 1/221 (0%) Frame = +3 Query: 3 DCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTN 182 DC FMVDNEA YDICRRNLDIERPTY NLNRLI Q+VSSITASLRF G+LNVDL EFQTN Sbjct: 203 DCTFMVDNEACYDICRRNLDIERPTYENLNRLIAQVVSSITASLRFAGSLNVDLNEFQTN 262 Query: 183 LVPYPRIHFPLVTYAPVISAEKAYMNSFPSPRSQTHASSPPTRW*NATPVMASTWLAVCC 362 LVPYPRIHFPLVTY+P++SA KA+ S P + P ++A C Sbjct: 263 LVPYPRIHFPLVTYSPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDP-RTGRYMATCL 321 Query: 363 TVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCPEATG-PRFNVXXXX 539 + ++ S S + P + PP +G + N Sbjct: 322 LYRGDVIPRDVQAAVTSIKSRRTIQFVDWCPTGFKIGICYEPPQHVPGSGIAKVNRAVCM 381 Query: 540 XXXXXXXXKLGARLDHKFRLMYAKAWLFVALVWSGDGMEAG 662 + +RLDHKF LMY+K FV + G+GME G Sbjct: 382 LSNTTSIAEAWSRLDHKFDLMYSKR-AFVHW-YVGEGMEEG 420 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 177 bits (432), Expect = 1e-45 Identities = 78/109 (71%), Positives = 89/109 (81%) Frame = +2 Query: 245 EGLHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKR 424 + HE SV EITN CFEP NQMVKCDPR G+YMA C+LYRGDV+P+DV AA+ TIK KR Sbjct: 280 KAFHESNSVQEITNQCFEPYNQMVKCDPRAGRYMATCLLYRGDVIPRDVQAAVTTIKAKR 339 Query: 425 TIQFVDWCPTGFKVGINYQPPTVVPGGDWAKVQRAVCMLSNTTAIAEAW 571 TIQFVDWCPTGFK+GI +PP + G + AKV RAVCMLSNTT+IAEAW Sbjct: 340 TIQFVDWCPTGFKIGICDRPPQHIEGSEIAKVDRAVCMLSNTTSIAEAW 388 Score = 167 bits (406), Expect = 1e-42 Identities = 97/221 (43%), Positives = 123/221 (55%), Gaps = 1/221 (0%) Frame = +3 Query: 3 DCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTN 182 DC FMVDNE+ YDICRRNLDIERP+Y NLNRLI Q+VSSITASLRF+G+LNVDL EFQTN Sbjct: 199 DCTFMVDNESCYDICRRNLDIERPSYENLNRLIAQVVSSITASLRFEGSLNVDLAEFQTN 258 Query: 183 LVPYPRIHFPLVTYAPVISAEKAYMNSFPSPRSQTHASSPPTRW*NATPVMASTWLAVCC 362 LVPYPRIHFPLVTYAP++SA KA+ S P + P A ++A C Sbjct: 259 LVPYPRIHFPLVTYAPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDP-RAGRYMATCL 317 Query: 363 TVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCPEATG-PRFNVXXXX 539 + ++ + + + P + PP E + + + Sbjct: 318 LYRGDVIPRDVQAAVTTIKAKRTIQFVDWCPTGFKIGICDRPPQHIEGSEIAKVDRAVCM 377 Query: 540 XXXXXXXXKLGARLDHKFRLMYAKAWLFVALVWSGDGMEAG 662 + +RLDHKF LMY+K FV + G+GME G Sbjct: 378 LSNTTSIAEAWSRLDHKFDLMYSKR-AFVHW-YVGEGMEEG 416 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 78.2 bits (184), Expect = 1e-15 Identities = 33/77 (42%), Positives = 50/77 (64%) Frame = +3 Query: 3 DCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTN 182 D F +DNEA+ I L I+ P+Y +LN L+ +++ +T S RF G LN DL + N Sbjct: 197 DETFCIDNEALSSIFANTLKIKSPSYDDLNHLVSAVMAGVTTSFRFPGELNSDLRKLAVN 256 Query: 183 LVPYPRIHFPLVTYAPV 233 +VP+PR+HF +V +AP+ Sbjct: 257 MVPFPRLHFFMVGFAPL 273 Score = 66.9 bits (156), Expect = 3e-12 Identities = 29/77 (37%), Positives = 44/77 (57%) Frame = +2 Query: 257 EQLSVAEITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQF 436 + +SV E+T F+ N MV DPRHG+Y+ L+RG V K+V+ I +++TK + F Sbjct: 282 QAVSVPELTQQMFDANNMMVAADPRHGRYLTVAALFRGKVSMKEVDEQIRSVQTKNSAYF 341 Query: 437 VDWCPTGFKVGINYQPP 487 V+W P + PP Sbjct: 342 VEWIPDNVLKAVCSVPP 358 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 61.7 bits (143), Expect = 1e-10 Identities = 27/76 (35%), Positives = 43/76 (56%) Frame = +3 Query: 3 DCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTN 182 D ++DN A+ I L + PT+ N+L+ ++S+ T +LR+ G +N DL + Sbjct: 200 DSVVVLDNAALAHIAADRLHTQNPTFHQQNQLVSTVMSASTTTLRYPGYMNNDLVSIIAS 259 Query: 183 LVPYPRIHFPLVTYAP 230 L+P PR HF L +Y P Sbjct: 260 LIPSPRCHFLLTSYTP 275 Score = 41.5 bits (93), Expect = 1e-04 Identities = 18/77 (23%), Positives = 40/77 (51%), Gaps = 1/77 (1%) Frame = +2 Query: 266 SVAEITNACFEPANQMVKCDP-RHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVD 442 +V ++ P NQMV +P + +++ + +G+ P DV+ ++ I+ +R F+ Sbjct: 291 TVLDVMRRLLLPKNQMVSVNPSKKSCFISILDIIQGEADPADVHKSLLRIRERRYASFIP 350 Query: 443 WCPTGFKVGINYQPPTV 493 W P +V ++ + P + Sbjct: 351 WGPASIQVALSKKSPYI 367 >SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharomyces pombe|chr 2|||Manual Length = 903 Score = 33.9 bits (74), Expect = 0.023 Identities = 18/54 (33%), Positives = 30/54 (55%) Frame = -2 Query: 191 RHQVSLELGEVHIQSAVESQRSSDRGDNLSDETIQVGVGWALNVEITAADVIDG 30 R + E + H ++ + Q+SSD ++ +D T VG+G AL I + D +DG Sbjct: 165 RRVLQAERAKEHRSNSNDKQKSSDNDEDPNDTT--VGIGAALAASILSVDSVDG 216 >SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 857 Score = 30.7 bits (66), Expect = 0.21 Identities = 16/45 (35%), Positives = 21/45 (46%) Frame = +3 Query: 366 VVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCP 500 VV P + RP++P P LS V PV+ V + PP P Sbjct: 552 VVPEAPSVPQRPAVPVVPEALSVPQPPVAPVAPEVPSVPQPPVAP 596 >SPCC18.03 |||shuttle craft like transcriptional regulator|Schizosaccharomyces pombe|chr 3|||Manual Length = 1077 Score = 30.3 bits (65), Expect = 0.28 Identities = 18/57 (31%), Positives = 23/57 (40%) Frame = +2 Query: 233 HLCREGLHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAI 403 H C++ H L ACFEP N C H K C L + KD N ++ Sbjct: 351 HTCKKRCHSGLC-----GACFEPINAKCYCG-LHSKTYPCSSLPSPSISKKDENGSV 401 >SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 800 Score = 30.3 bits (65), Expect = 0.28 Identities = 25/91 (27%), Positives = 42/91 (46%), Gaps = 3/91 (3%) Frame = +3 Query: 219 TYAPVISAEKAYMNSFP---SPRSQTHASSPPTRW*NATPVMASTWLAVCCTVVTSYPRM 389 T PV S+ +S P +P + T+ ++ + +TPV ++ CT TS P Sbjct: 505 TSTPVTSSNYTISSSTPVTSTPVTTTNCTTSTSVLYTSTPVTSTPLATTNCTTSTSVPYT 564 Query: 390 *TRPSLPSKPSVLSNSSTGVQPVSRSVSTTS 482 T P S ++ S++ PV+ + TTS Sbjct: 565 ST-PVTSSNYTISSSTPVTSTPVTTTNCTTS 594 Score = 26.6 bits (56), Expect = 3.5 Identities = 20/76 (26%), Positives = 32/76 (42%) Frame = +3 Query: 261 SFPSPRSQTHASSPPTRW*NATPVMASTWLAVCCTVVTSYPRM*TRPSLPSKPSVLSNSS 440 S +P + T+ ++ + +TPV ++ CT TS P T + S P +N + Sbjct: 417 SSSTPLTTTNCTTSTSVPYTSTPVTSTPLATTNCTTSTSVPY--TSTPVTSTPLTTTNCT 474 Query: 441 TGVQPVSRSVSTTSHP 488 T S TS P Sbjct: 475 TSTSIPYTSTPVTSTP 490 Score = 26.2 bits (55), Expect = 4.6 Identities = 26/104 (25%), Positives = 40/104 (38%), Gaps = 2/104 (1%) Frame = +3 Query: 177 TNLVPYPRIHFPLVTYAPVISAEKAYMNS--FPSPRSQTHASSPPTRW*NATPVMASTWL 350 T VP PL T S Y ++ +P + T+ ++ + +TPV ++ Sbjct: 410 TPTVPPTSSSTPLTTTNCTTSTSVPYTSTPVTSTPLATTNCTTSTSVPYTSTPVTSTPLT 469 Query: 351 AVCCTVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTS 482 CT TS P T + S P +N +T S TS Sbjct: 470 TTNCTTSTSIPY--TSTPVTSTPLTTTNCTTSTSVPYTSTPVTS 511 >SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1|||Manual Length = 3227 Score = 28.3 bits (60), Expect = 1.1 Identities = 14/48 (29%), Positives = 23/48 (47%) Frame = -3 Query: 406 SDGRVHILGYDVTTVQHTASHVLAMTGVAFHHLVGGLEACVCDLGDGK 263 S G +LGY ++ A++V+A + V HL+ G D + K Sbjct: 407 SAGLTSLLGYHLSVKTPQATYVVARSIVMLDHLIDGYSMAFPDFSESK 454 >SPBC428.07 |meu6||meiotic chromosome segregation protein Meu6|Schizosaccharomyces pombe|chr 2|||Manual Length = 651 Score = 28.3 bits (60), Expect = 1.1 Identities = 20/64 (31%), Positives = 28/64 (43%), Gaps = 2/64 (3%) Frame = +3 Query: 147 ALNVDLTEFQTNLVPYPRIHFPLVT--YAPVISAEKAYMNSFPSPRSQTHASSPPTRW*N 320 A +V++ E +TN +P HFP T AP S EK S + T+ N Sbjct: 368 AQSVEVPENETNQIPTTEEHFPATTEEVAPA-SEEKPATGPAEESTSTQNVEQASTQNDN 426 Query: 321 ATPV 332 TP+ Sbjct: 427 GTPI 430 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 28.3 bits (60), Expect = 1.1 Identities = 16/42 (38%), Positives = 21/42 (50%) Frame = +3 Query: 186 VPYPRIHFPLVTYAPVISAEKAYMNSFPSPRSQTHASSPPTR 311 VP P P V+ P S + N+ PSP S + AS+ P R Sbjct: 1239 VPAPSSEAPSVS-TPRSSVPSPHSNASPSPTSSSMASAAPAR 1279 >SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|chr 1|||Manual Length = 628 Score = 26.2 bits (55), Expect = 4.6 Identities = 14/32 (43%), Positives = 21/32 (65%), Gaps = 3/32 (9%) Frame = +3 Query: 402 SLPSKPSV---LSNSSTGVQPVSRSVSTTSHP 488 +LP KPS+ +++S V+P S STTS+P Sbjct: 5 TLPPKPSISPSIASSFPTVKPFSSQNSTTSNP 36 >SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosaccharomyces pombe|chr 1|||Manual Length = 157 Score = 25.8 bits (54), Expect = 6.1 Identities = 10/20 (50%), Positives = 11/20 (55%) Frame = -2 Query: 383 WVRRHHGTAYSKPCTCHDGG 324 W R H Y+KPC DGG Sbjct: 16 WRRDHPFGFYAKPCKSSDGG 35 >SPCC320.07c |mde7||RNA-binding protein Mde7|Schizosaccharomyces pombe|chr 3|||Manual Length = 761 Score = 25.8 bits (54), Expect = 6.1 Identities = 15/62 (24%), Positives = 28/62 (45%), Gaps = 1/62 (1%) Frame = +3 Query: 138 FDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKAYMNSFPSPRSQTHASSP-PTRW 314 FD +N D+T YP + ++ P+ + + Y+N +S+ +P P+ W Sbjct: 336 FDSVINKDVTSESRMSTSYPAVLEAFKSFNPIKAPKAEYLN-----QSRASMGNPSPSNW 390 Query: 315 *N 320 N Sbjct: 391 NN 392 >SPAC6G9.04 |mug79||meiotically upregulated gene Mug79|Schizosaccharomyces pombe|chr 1|||Manual Length = 1318 Score = 25.4 bits (53), Expect = 8.0 Identities = 15/58 (25%), Positives = 27/58 (46%) Frame = -2 Query: 185 QVSLELGEVHIQSAVESQRSSDRGDNLSDETIQVGVGWALNVEITAADVIDGFIVDHE 12 Q ++ VH ++A E R N + QVG +N ++ D+ + F+ D+E Sbjct: 601 QSQIKSQSVHHENAAEEIRKMRNLRNSRINSQQVGEKVFVNPDVKTMDIQETFLQDYE 658 >SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces pombe|chr 1|||Manual Length = 665 Score = 25.4 bits (53), Expect = 8.0 Identities = 13/34 (38%), Positives = 19/34 (55%), Gaps = 2/34 (5%) Frame = +3 Query: 399 PSLPSKPSVLSNSST--GVQPVSRSVSTTSHPPW 494 P PS+P+++SN ST G+Q V V + W Sbjct: 531 PISPSRPALISNISTKKGIQVVGNMVYDPTRLRW 564 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,241,627 Number of Sequences: 5004 Number of extensions: 69859 Number of successful extensions: 289 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 262 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 289 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 329179816 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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