Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30497
(717 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 186 4e-48
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 186 4e-48
SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 118 1e-27
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 56 4e-09
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 30 0.38
SPBC800.10c |||EPS15 repeat family actin cortical patch componen... 29 0.88
SPAC22F3.09c |res2|mcs1, pct1|MBF transcription factor complex s... 28 1.5
SPCC1906.01 |mpg1||mannose-1-phosphate guanyltransferase Mpg1|Sc... 28 1.5
SPBC32F12.09 |rum1||CDK inhibitor Rum1|Schizosaccharomyces pombe... 27 3.5
SPBC36.07 |iki3||RNA polymerase II elongator subunit Iki3 |Schiz... 26 4.7
SPBC1734.15 |rsc4|brd1|RSC complex subunit Rsc4|Schizosaccharomy... 26 4.7
SPAC1006.08 |etd1||ethanol-hypersensitive mutant protein |Schizo... 26 4.7
SPBC14F5.13c |||alkaline phosphatase |Schizosaccharomyces pombe|... 26 4.7
SPCC895.08c |||conserved fungal protein|Schizosaccharomyces pomb... 25 8.2
SPBC19G7.01c |msh2|swi8, mut3, SPBC24C6.12c|MutS protein homolog... 22 9.6
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 186 bits (452), Expect = 4e-48
Identities = 112/235 (47%), Positives = 140/235 (59%), Gaps = 3/235 (1%)
Frame = +1
Query: 1 PMPDGLPEDIDEGRVNPRDDFKTRARYLTEKYEYDVTEARKIWCFGPEGTGPQHPGRLLQ 180
PM + L I+ G VNPRDDFK RAR + +++ +DVT+ARKIWCFGP+ TG + Q
Sbjct: 592 PMSEELSVAIETGHVNPRDDFKVRARIMADEFGWDVTDARKIWCFGPDTTGANVV--VDQ 649
Query: 181 RSSVPQ*N*---GLCCGWIPVGR*GRS*AEENLRGVRFNIYDVTLHTDAIHRGGGQIIPT 351
+V N + + + G EENLR RFNI DV LH DAIHRGGGQIIPT
Sbjct: 650 TKAVAYLNEIKDSVVAAFAWASKEGPM-FEENLRSCRFNILDVVLHADAIHRGGGQIIPT 708
Query: 352 TRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVEVHPCSL* 531
RR +YA L A P + EPV+L EIQ E A+GGIY VLN++RGHVF E Q P
Sbjct: 709 ARRVVYASTLLASPIIQEPVFLVEIQVSENAMGGIYSVLNKKRGHVFSEEQRVGTPLYNI 768
Query: 532 KAYLPVNEVVRFYCRFCGSNNGRNKAFPANA*FEPFGKVPPLGEPCEPSRTKP*Q 696
KAYLPVNE F + G +AFP F+ + P G+P +P+ +KP Q
Sbjct: 769 KAYLPVNESFGFTGELRQATAG--QAFPQLV-FDHWS--PMSGDPLDPT-SKPGQ 817
Score = 51.6 bits (118), Expect = 1e-07
Identities = 23/37 (62%), Positives = 27/37 (72%)
Frame = +2
Query: 140 PRVPDPNILVDCSKGVQYLNEIKDSVVAGFQWAAKEG 250
P N++VD +K V YLNEIKDSVVA F WA+KEG
Sbjct: 638 PDTTGANVVVDQTKAVAYLNEIKDSVVAAFAWASKEG 674
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 186 bits (452), Expect = 4e-48
Identities = 112/235 (47%), Positives = 140/235 (59%), Gaps = 3/235 (1%)
Frame = +1
Query: 1 PMPDGLPEDIDEGRVNPRDDFKTRARYLTEKYEYDVTEARKIWCFGPEGTGPQHPGRLLQ 180
PM + L I+ G VNPRDDFK RAR + +++ +DVT+ARKIWCFGP+ TG + Q
Sbjct: 592 PMSEELSVAIETGHVNPRDDFKVRARIMADEFGWDVTDARKIWCFGPDTTGANVV--VDQ 649
Query: 181 RSSVPQ*N*---GLCCGWIPVGR*GRS*AEENLRGVRFNIYDVTLHTDAIHRGGGQIIPT 351
+V N + + + G EENLR RFNI DV LH DAIHRGGGQIIPT
Sbjct: 650 TKAVAYLNEIKDSVVAAFAWASKEGPM-FEENLRSCRFNILDVVLHADAIHRGGGQIIPT 708
Query: 352 TRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVEVHPCSL* 531
RR +YA L A P + EPV+L EIQ E A+GGIY VLN++RGHVF E Q P
Sbjct: 709 ARRVVYASTLLASPIIQEPVFLVEIQVSENAMGGIYSVLNKKRGHVFSEEQRVGTPLYNI 768
Query: 532 KAYLPVNEVVRFYCRFCGSNNGRNKAFPANA*FEPFGKVPPLGEPCEPSRTKP*Q 696
KAYLPVNE F + G +AFP F+ + P G+P +P+ +KP Q
Sbjct: 769 KAYLPVNESFGFTGELRQATAG--QAFPQLV-FDHWS--PMSGDPLDPT-SKPGQ 817
Score = 51.6 bits (118), Expect = 1e-07
Identities = 23/37 (62%), Positives = 27/37 (72%)
Frame = +2
Query: 140 PRVPDPNILVDCSKGVQYLNEIKDSVVAGFQWAAKEG 250
P N++VD +K V YLNEIKDSVVA F WA+KEG
Sbjct: 638 PDTTGANVVVDQTKAVAYLNEIKDSVVAAFAWASKEG 674
>SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 983
Score = 118 bits (283), Expect = 1e-27
Identities = 69/188 (36%), Positives = 99/188 (52%), Gaps = 5/188 (2%)
Frame = +1
Query: 1 PMPDGLPEDIDEGRVNPRDDFKTRARYLTEKYEYDVTEARKIWCFGPEGTGPQHPGRLLQ 180
P+ G+ DI+ G+VN K + + + Y++D+ +R IW FGP+ G
Sbjct: 704 PLEKGISNDIENGKVNINWPQKRISEFFQKNYDWDLLASRSIWAFGPDDRGTNILRDDTL 763
Query: 181 RSSVPQ*N*GLCCGWIPVG-----R*GRS*AEENLRGVRFNIYDVTLHTDAIHRGGGQII 345
+ V + +I G R G +E +R V F + DV L + I+RGGGQII
Sbjct: 764 STDVDKNVLNSVKEYIKQGFQWGTREGPL-CDETIRNVNFRLMDVVLAPEQIYRGGGQII 822
Query: 346 PTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVEVHPCS 525
PT RR Y+ LTA PRLMEPVY+ E+ P ++ IY +L RRRGHV ++ P
Sbjct: 823 PTARRVCYSSFLTASPRLMEPVYMVEVHAPADSLPIIYDLLTRRRGHVLQDIPRPGSPLY 882
Query: 526 L*KAYLPV 549
L +A +PV
Sbjct: 883 LVRALIPV 890
Score = 30.3 bits (65), Expect = 0.29
Identities = 13/33 (39%), Positives = 18/33 (54%)
Frame = +2
Query: 152 DPNILVDCSKGVQYLNEIKDSVVAGFQWAAKEG 250
D + D K V LN +K+ + GFQW +EG
Sbjct: 760 DDTLSTDVDKNV--LNSVKEYIKQGFQWGTREG 790
>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Manual
Length = 1000
Score = 56.4 bits (130), Expect = 4e-09
Identities = 28/78 (35%), Positives = 42/78 (53%)
Frame = +1
Query: 334 GQIIPTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVEV 513
GQ+I + + L PRLM +Y C++Q +G +YGV+++RRG V +E E
Sbjct: 841 GQVISVVKESIRHGFLGWSPRLMLAMYSCDVQATSEVLGRVYGVVSKRRGRVIDEEMKEG 900
Query: 514 HPCSL*KAYLPVNEVVRF 567
P + KA +PV E F
Sbjct: 901 TPFFIVKALIPVVESFGF 918
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 29.9 bits (64), Expect = 0.38
Identities = 17/63 (26%), Positives = 33/63 (52%)
Frame = +1
Query: 379 LTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVEVHPCSL*KAYLPVNEV 558
L A P ++EP+ I P GG+ G L++R+ + +S + +L +A +P+N +
Sbjct: 668 LQANPMVLEPIMNVSITAPVEHQGGVIGNLDKRKATIV-DSDTDEDEFTL-QAEVPLNSM 725
Query: 559 VRF 567
+
Sbjct: 726 FSY 728
>SPBC800.10c |||EPS15 repeat family actin cortical patch component
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1116
Score = 28.7 bits (61), Expect = 0.88
Identities = 15/57 (26%), Positives = 27/57 (47%)
Frame = +2
Query: 545 LSMKSFGFTADFAVPTTGGTRPSPQMRNSNHLARSLPWENRANLPEPNPNKVVQGKP 715
+ +K G +P++ + +P M+ S + S+P AN+ PNPN + P
Sbjct: 324 IKLKLSGKELPKVLPSSMLSSVAPLMQKSKSVPTSIPSVVPANISSPNPNPTLAPNP 380
>SPAC22F3.09c |res2|mcs1, pct1|MBF transcription factor complex
subunit Res2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 657
Score = 27.9 bits (59), Expect = 1.5
Identities = 12/23 (52%), Positives = 15/23 (65%)
Frame = -3
Query: 277 HHANSLQPNSFLSGPLESSHNRV 209
H N QPNS +S +ESS N+V
Sbjct: 144 HSVNEKQPNSSISPTIESSMNKV 166
>SPCC1906.01 |mpg1||mannose-1-phosphate guanyltransferase
Mpg1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 363
Score = 27.9 bits (59), Expect = 1.5
Identities = 18/56 (32%), Positives = 30/56 (53%)
Frame = +2
Query: 158 NILVDCSKGVQYLNEIKDSVVAGFQWAAKEGVRLKRICVVFDSTSMM*HSILMPSI 325
N+L+D S + +I +VV G +GVRL+R C + S+ + H+ + SI
Sbjct: 255 NVLIDPSATIGKNCKIGPNVVIGPNVTIGDGVRLQR-CAILKSSRVRDHAWVKSSI 309
>SPBC32F12.09 |rum1||CDK inhibitor Rum1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 230
Score = 26.6 bits (56), Expect = 3.5
Identities = 11/26 (42%), Positives = 16/26 (61%)
Frame = -1
Query: 144 LGAKAPNLTGFGNIVFVLFCKIPSAS 67
+G PNL GN +F L ++PS+S
Sbjct: 169 IGILPPNLNSIGNKMFSLKSRVPSSS 194
>SPBC36.07 |iki3||RNA polymerase II elongator subunit Iki3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1253
Score = 26.2 bits (55), Expect = 4.7
Identities = 11/18 (61%), Positives = 14/18 (77%)
Frame = +1
Query: 16 LPEDIDEGRVNPRDDFKT 69
LPE IDEG+++ DD KT
Sbjct: 194 LPEKIDEGKLSDVDDGKT 211
>SPBC1734.15 |rsc4|brd1|RSC complex subunit Rsc4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 542
Score = 26.2 bits (55), Expect = 4.7
Identities = 17/67 (25%), Positives = 30/67 (44%), Gaps = 1/67 (1%)
Frame = +1
Query: 343 IPTTRRCLYACLLTAQPRLMEPVYL-CEIQCPEVAVGGIYGVLNRRRGHVFEESQVEVHP 519
IP T RC+ + + + P+L P L + CP + + V+ H F + ++P
Sbjct: 386 IPNTERCILSAFICSPPQLPLPNPLRMYLPCPSLNSTEV-SVITLAPQHSFLNIVINLNP 444
Query: 520 CSL*KAY 540
K+Y
Sbjct: 445 ALALKSY 451
>SPAC1006.08 |etd1||ethanol-hypersensitive mutant protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 391
Score = 26.2 bits (55), Expect = 4.7
Identities = 15/49 (30%), Positives = 26/49 (53%), Gaps = 1/49 (2%)
Frame = -1
Query: 408 RLHETGLSS*QTCIQASSS-CWNDLATTSMDGISMECYIIDVESNTTQI 265
++ ET L + ++S CW++L+TTS + + II + TT I
Sbjct: 191 QIQETSLGATTQEDESSDGICWDELSTTSPESSKVSEPIIQDNTQTTHI 239
>SPBC14F5.13c |||alkaline phosphatase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 532
Score = 26.2 bits (55), Expect = 4.7
Identities = 13/31 (41%), Positives = 18/31 (58%)
Frame = +2
Query: 68 LALGILQKSTNTMLPKPVRFGALAPRVPDPN 160
LA I+ + NT+LP +F A +PDPN
Sbjct: 388 LASAIVNHNQNTLLPYIEQFVLPAIGIPDPN 418
>SPCC895.08c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 490
Score = 25.4 bits (53), Expect = 8.2
Identities = 14/45 (31%), Positives = 21/45 (46%)
Frame = +1
Query: 481 GHVFEESQVEVHPCSL*KAYLPVNEVVRFYCRFCGSNNGRNKAFP 615
G V + Q HPCS+ K + FYC++ ++ K FP
Sbjct: 12 GAVSDTFQEVQHPCSILKYKVYFVTTDTFYCKYLTASFTGLKKFP 56
>SPBC19G7.01c |msh2|swi8, mut3, SPBC24C6.12c|MutS protein homolog
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 982
Score = 22.2 bits (45), Expect(2) = 9.6
Identities = 9/21 (42%), Positives = 11/21 (52%)
Frame = -3
Query: 379 ADMHTSIF*LLE*FGHHLYGW 317
+D+H L HHLYGW
Sbjct: 517 SDLHQDTEKKLHLEQHHLYGW 537
Score = 21.0 bits (42), Expect(2) = 9.6
Identities = 9/24 (37%), Positives = 12/24 (50%)
Frame = -3
Query: 496 LRKRDHDVCSAHRRYHPQLLQDTE 425
L + H + H+R L QDTE
Sbjct: 501 LDELQHSIFEEHKRVGSDLHQDTE 524
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,393,076
Number of Sequences: 5004
Number of extensions: 77170
Number of successful extensions: 203
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 192
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 202
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 335201398
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -