Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30497
(717 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 192 3e-49
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 121 4e-28
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 121 4e-28
At5g25230.1 68418.m02991 elongation factor Tu family protein tra... 114 6e-26
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 52 3e-07
At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 37 0.015
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 36 0.035
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 36 0.035
At5g13650.2 68418.m01585 elongation factor family protein contai... 32 0.44
At5g13650.1 68418.m01584 elongation factor family protein contai... 32 0.44
At4g24290.2 68417.m03488 expressed protein 31 0.58
At4g24290.1 68417.m03487 expressed protein 31 0.58
At5g15580.1 68418.m01824 expressed protein unknown protein F14P3... 29 3.1
At5g39900.1 68418.m04839 GTP-binding protein LepA, putative GTP-... 29 4.1
At2g25730.1 68415.m03084 expressed protein 28 7.1
At1g56130.1 68414.m06445 leucine-rich repeat family protein / pr... 27 9.4
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 192 bits (467), Expect = 3e-49
Identities = 101/206 (49%), Positives = 134/206 (65%), Gaps = 1/206 (0%)
Frame = +1
Query: 1 PMPDGLPEDIDEGRVNPRDDFKTRARYLTEKYEYDVTEARKIWCFGPEGTGPQHPGRLLQ 180
PM +GL E ID+GR+ PRDD K R++ L E++ +D A+KIW FGPE TGP + +
Sbjct: 593 PMEEGLAEAIDDGRIGPRDDPKIRSKILAEEFGWDKDLAKKIWAFGPETTGPNMVVDMCK 652
Query: 181 RSS-VPQ*N*GLCCGWIPVGR*GRS*AEENLRGVRFNIYDVTLHTDAIHRGGGQIIPTTR 357
+ + + G+ + G AEEN+RG+ F + DV LH+DAIHRGGGQ+IPT R
Sbjct: 653 GVQYLNEIKDSVVAGFQWASKEGPL-AEENMRGICFEVCDVVLHSDAIHRGGGQVIPTAR 711
Query: 358 RCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVEVHPCSL*KA 537
R +YA +TA+PRL+EPVY+ EIQ PE A+GGIY VLN++RGHVFEE Q P KA
Sbjct: 712 RVIYASQITAKPRLLEPVYMVEIQAPEGALGGIYSVLNQKRGHVFEEMQRPGTPLYNIKA 771
Query: 538 YLPVNEVVRFYCRFCGSNNGRNKAFP 615
YLPV E F + + +G +AFP
Sbjct: 772 YLPVVESFGFSSQLRAATSG--QAFP 795
Score = 66.1 bits (154), Expect = 2e-11
Identities = 29/40 (72%), Positives = 32/40 (80%)
Frame = +2
Query: 131 ALAPRVPDPNILVDCSKGVQYLNEIKDSVVAGFQWAAKEG 250
A P PN++VD KGVQYLNEIKDSVVAGFQWA+KEG
Sbjct: 636 AFGPETTGPNMVVDMCKGVQYLNEIKDSVVAGFQWASKEG 675
>At1g06220.2 68414.m00656 elongation factor Tu family protein similar
to Cryptosporidium parvum elongation factor-2 GB:U21667
GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 121 bits (292), Expect = 4e-28
Identities = 76/195 (38%), Positives = 102/195 (52%), Gaps = 6/195 (3%)
Frame = +1
Query: 1 PMPDGLPEDIDEGRVNPRDDFKTRARYLTEKYEYDVTEARKIWCFGPEGTGPQH------ 162
P+ GL EDI+ G V+ + K + KY++D+ AR IW FGP+ GP
Sbjct: 705 PLDRGLAEDIENGVVSIDWNRKQLGDFFRTKYDWDLLAARSIWAFGPDKQGPNILLDDTL 764
Query: 163 PGRLLQRSSVPQ*N*GLCCGWIPVGR*GRS*AEENLRGVRFNIYDVTLHTDAIHRGGGQI 342
P + R+ + + G+ R G +E +R V+F I D + + +HRG GQ+
Sbjct: 765 PTEV-DRNLMMAVKDSIVQGFQWGAREGPL-CDEPIRNVKFKIVDARIAPEPLHRGSGQM 822
Query: 343 IPTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVEVHPC 522
IPT RR Y+ L A PRLMEPVY EIQ P V IY VL+RRRGHV + P
Sbjct: 823 IPTARRVAYSAFLMATPRLMEPVYYVEIQTPIDCVTAIYTVLSRRRGHVTSDVPQPGTPA 882
Query: 523 SL*KAYLPVNEVVRF 567
+ KA+LPV E F
Sbjct: 883 YIVKAFLPVIESFGF 897
Score = 35.9 bits (79), Expect = 0.027
Identities = 18/44 (40%), Positives = 25/44 (56%), Gaps = 4/44 (9%)
Frame = +2
Query: 131 ALAPRVPDPNILVDCSKGVQY----LNEIKDSVVAGFQWAAKEG 250
A P PNIL+D + + + +KDS+V GFQW A+EG
Sbjct: 748 AFGPDKQGPNILLDDTLPTEVDRNLMMAVKDSIVQGFQWGAREG 791
>At1g06220.1 68414.m00655 elongation factor Tu family protein similar
to Cryptosporidium parvum elongation factor-2 GB:U21667
GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 121 bits (292), Expect = 4e-28
Identities = 76/195 (38%), Positives = 102/195 (52%), Gaps = 6/195 (3%)
Frame = +1
Query: 1 PMPDGLPEDIDEGRVNPRDDFKTRARYLTEKYEYDVTEARKIWCFGPEGTGPQH------ 162
P+ GL EDI+ G V+ + K + KY++D+ AR IW FGP+ GP
Sbjct: 705 PLDRGLAEDIENGVVSIDWNRKQLGDFFRTKYDWDLLAARSIWAFGPDKQGPNILLDDTL 764
Query: 163 PGRLLQRSSVPQ*N*GLCCGWIPVGR*GRS*AEENLRGVRFNIYDVTLHTDAIHRGGGQI 342
P + R+ + + G+ R G +E +R V+F I D + + +HRG GQ+
Sbjct: 765 PTEV-DRNLMMAVKDSIVQGFQWGAREGPL-CDEPIRNVKFKIVDARIAPEPLHRGSGQM 822
Query: 343 IPTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVEVHPC 522
IPT RR Y+ L A PRLMEPVY EIQ P V IY VL+RRRGHV + P
Sbjct: 823 IPTARRVAYSAFLMATPRLMEPVYYVEIQTPIDCVTAIYTVLSRRRGHVTSDVPQPGTPA 882
Query: 523 SL*KAYLPVNEVVRF 567
+ KA+LPV E F
Sbjct: 883 YIVKAFLPVIESFGF 897
Score = 35.9 bits (79), Expect = 0.027
Identities = 18/44 (40%), Positives = 25/44 (56%), Gaps = 4/44 (9%)
Frame = +2
Query: 131 ALAPRVPDPNILVDCSKGVQY----LNEIKDSVVAGFQWAAKEG 250
A P PNIL+D + + + +KDS+V GFQW A+EG
Sbjct: 748 AFGPDKQGPNILLDDTLPTEVDRNLMMAVKDSIVQGFQWGAREG 791
>At5g25230.1 68418.m02991 elongation factor Tu family protein
translation Elongation Factor 2, Schizosaccharomyces
pombe, PIR:T39902
Length = 973
Score = 114 bits (274), Expect = 6e-26
Identities = 74/194 (38%), Positives = 101/194 (52%), Gaps = 5/194 (2%)
Frame = +1
Query: 1 PMPDGLPEDIDEGRVNPRDDFKTRARYLTEKYEYDVTEARKIWCFGPE--GTGPQHPGRL 174
P+ GL EDI+ G V+ + + KY++D+ AR IW FGP+ GT L
Sbjct: 691 PLDRGLAEDIENGVVSIDWNRVQLGDFFRTKYDWDLLAARSIWAFGPDKQGTNILLDDTL 750
Query: 175 ---LQRSSVPQ*N*GLCCGWIPVGR*GRS*AEENLRGVRFNIYDVTLHTDAIHRGGGQII 345
+ R+ + + G+ R G +E +R V+F I D + + +HRG GQ+I
Sbjct: 751 PTEVDRNLMMGVKDSIVQGFQWGAREGPL-CDEPIRNVKFKIVDARIAPEPLHRGSGQMI 809
Query: 346 PTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVEVHPCS 525
PT RR Y+ L A PRLMEPVY EIQ P V IY VL+RRRG+V + P
Sbjct: 810 PTARRVAYSAFLMATPRLMEPVYYVEIQTPIDCVTAIYTVLSRRRGYVTSDVPQPGTPAY 869
Query: 526 L*KAYLPVNEVVRF 567
+ KA+LPV E F
Sbjct: 870 IVKAFLPVIESFGF 883
Score = 32.3 bits (70), Expect = 0.33
Identities = 17/44 (38%), Positives = 24/44 (54%), Gaps = 4/44 (9%)
Frame = +2
Query: 131 ALAPRVPDPNILVDCSKGVQY----LNEIKDSVVAGFQWAAKEG 250
A P NIL+D + + + +KDS+V GFQW A+EG
Sbjct: 734 AFGPDKQGTNILLDDTLPTEVDRNLMMGVKDSIVQGFQWGAREG 777
>At3g22980.1 68416.m02898 elongation factor Tu family protein similar
to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 52.4 bits (120), Expect = 3e-07
Identities = 25/78 (32%), Positives = 40/78 (51%)
Frame = +1
Query: 334 GQIIPTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVEV 513
GQ++ + A +L PR++E +Y CE+ +G +Y VL+RRR + +E E
Sbjct: 853 GQVMTAVKDACRAAVLQTNPRIVEAMYFCELNTAPEYLGPMYAVLSRRRARILKEEMQEG 912
Query: 514 HPCSL*KAYLPVNEVVRF 567
AY+PV+E F
Sbjct: 913 SSLFTVHAYVPVSESFGF 930
>At1g62750.1 68414.m07082 elongation factor Tu family protein
similar to elongation factor G SP:P34811 [Glycine max
(Soybean)]
Length = 783
Score = 36.7 bits (81), Expect = 0.015
Identities = 15/35 (42%), Positives = 22/35 (62%)
Frame = +1
Query: 385 AQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHV 489
A PR++EP+ E+ PE +G + G LN RRG +
Sbjct: 683 AGPRMLEPIMRVEVVTPEEHLGDVIGDLNSRRGQI 717
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 35.5 bits (78), Expect = 0.035
Identities = 15/44 (34%), Positives = 25/44 (56%)
Frame = +1
Query: 373 CLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQ 504
C A+P ++EPV L E++ P G + G +N+R+G + Q
Sbjct: 649 CYTAARPVILEPVMLVELKVPTEFQGTVAGDINKRKGIIVGNDQ 692
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 35.5 bits (78), Expect = 0.035
Identities = 15/44 (34%), Positives = 25/44 (56%)
Frame = +1
Query: 373 CLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQ 504
C A+P ++EPV L E++ P G + G +N+R+G + Q
Sbjct: 649 CYTAARPVILEPVMLVELKVPTEFQGTVAGDINKRKGIIVGNDQ 692
>At5g13650.2 68418.m01585 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 676
Score = 31.9 bits (69), Expect = 0.44
Identities = 11/34 (32%), Positives = 22/34 (64%)
Frame = +1
Query: 394 RLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFE 495
+L+EP + ++ PE +G + +L +RRG +F+
Sbjct: 473 KLLEPYEIATVEVPEAHMGPVVELLGKRRGQMFD 506
>At5g13650.1 68418.m01584 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 675
Score = 31.9 bits (69), Expect = 0.44
Identities = 11/34 (32%), Positives = 22/34 (64%)
Frame = +1
Query: 394 RLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFE 495
+L+EP + ++ PE +G + +L +RRG +F+
Sbjct: 472 KLLEPYEIATVEVPEAHMGPVVELLGKRRGQMFD 505
>At4g24290.2 68417.m03488 expressed protein
Length = 606
Score = 31.5 bits (68), Expect = 0.58
Identities = 21/79 (26%), Positives = 38/79 (48%), Gaps = 4/79 (5%)
Frame = -1
Query: 357 SSCWN-DLATT---SMDGISMECYIIDVESNTTQILFSLTPSLAAHWNPATTESLISLRY 190
SSCW D A T + DG+ + Y + ++ + + + ++ + W+PA I + Y
Sbjct: 115 SSCWQKDAAYTKNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDI-Y 173
Query: 189 *TPLEQSTRMLGSGTLGAK 133
T + S +M G + AK
Sbjct: 174 GTHIIVSVKMGGKDVIYAK 192
>At4g24290.1 68417.m03487 expressed protein
Length = 350
Score = 31.5 bits (68), Expect = 0.58
Identities = 21/79 (26%), Positives = 38/79 (48%), Gaps = 4/79 (5%)
Frame = -1
Query: 357 SSCWN-DLATT---SMDGISMECYIIDVESNTTQILFSLTPSLAAHWNPATTESLISLRY 190
SSCW D A T + DG+ + Y + ++ + + + ++ + W+PA I + Y
Sbjct: 115 SSCWQKDAAYTKNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDI-Y 173
Query: 189 *TPLEQSTRMLGSGTLGAK 133
T + S +M G + AK
Sbjct: 174 GTHIIVSVKMGGKDVIYAK 192
>At5g15580.1 68418.m01824 expressed protein unknown protein F14P3.18
- Arabidopsis thaliana, EMBL:AC009755
Length = 927
Score = 29.1 bits (62), Expect = 3.1
Identities = 18/45 (40%), Positives = 25/45 (55%)
Frame = +3
Query: 42 RKSPR*LQDSRSVSYRKVRIRCYRSP*DLVLWPRGYRTPTSWSIA 176
+++PR DSRS S+R R C P +LV G+R TS +A
Sbjct: 223 KETPRLSLDSRSNSFRSARSSCSPEPQELV---TGHRRTTSSVVA 264
>At5g39900.1 68418.m04839 GTP-binding protein LepA, putative
GTP-binding protein GUF1 - Saccharomyces cerevisiae,
PIR:S50374
Length = 661
Score = 28.7 bits (61), Expect = 4.1
Identities = 19/57 (33%), Positives = 27/57 (47%), Gaps = 1/57 (1%)
Frame = +1
Query: 403 EPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVEVHPCSL*KAYLPVNE-VVRFY 570
EP + I P VG + + + RRG E + ++ L K LP+ E VV FY
Sbjct: 466 EPTVIATIILPSEYVGAVINLCSDRRGQQLEYTFIDAQRVFL-KYQLPLREIVVDFY 521
>At2g25730.1 68415.m03084 expressed protein
Length = 2464
Score = 27.9 bits (59), Expect = 7.1
Identities = 17/44 (38%), Positives = 21/44 (47%), Gaps = 2/44 (4%)
Frame = +3
Query: 126 LVLWPRGYRTPTS--WSIAPKEFSTSMKLRTLLWLDSSGPLRKE 251
L L+PR S W + P + +T KL LLW S LR E
Sbjct: 419 LTLFPRLRPLVASMGWDLLPGKTATRRKLMRLLWTSDSQALRLE 462
>At1g56130.1 68414.m06445 leucine-rich repeat family protein /
protein kinase family protein contains Pfam domains
PF00560: Leucine Rich Repeat and PF00069: Protein kinase
domain
Length = 1032
Score = 27.5 bits (58), Expect = 9.4
Identities = 19/53 (35%), Positives = 25/53 (47%), Gaps = 1/53 (1%)
Frame = -1
Query: 714 GFPCTTLLGFGSGRFARFSQGRDLAKWFEL-RICGEGLVPPVVGTAKSAVKPN 559
G TT G G RF + QGR + K F++ R G+ V V K+ V N
Sbjct: 532 GSTSTTWKGLGRRRFDIYVQGRLVEKDFDVRRTAGDSTVRAVQRVYKANVSEN 584
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,403,912
Number of Sequences: 28952
Number of extensions: 439927
Number of successful extensions: 1236
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 1198
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1236
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1555552968
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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