BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30494 (788 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 188 8e-49 SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 188 8e-49 SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 135 6e-33 SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 47 3e-06 SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 30 0.43 SPCC320.12 ||SPCC330.17c|mitochondrial inner membrane peptidase ... 28 1.8 SPBC19C2.15c ||SPBC2F12.16|phosphoprotein phosphatase |Schizosac... 27 3.1 SPBC36.07 |iki3||RNA polymerase II elongator subunit Iki3 |Schiz... 27 4.0 SPAC1006.08 |etd1||ethanol-hypersensitive mutant protein |Schizo... 27 4.0 SPBC32F12.09 |rum1||CDK inhibitor Rum1|Schizosaccharomyces pombe... 27 4.0 >SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 842 Score = 188 bits (458), Expect = 8e-49 Identities = 103/224 (45%), Positives = 129/224 (57%), Gaps = 1/224 (0%) Frame = +2 Query: 2 FMKAQPMPDGLPEDIDEGRVNPRDDFKTRARYLTEKYEYDVTEARKIWCFGPEGTGPQHP 181 FM A+PM + L I+ G VNPRDDFK RAR + +++ +DVT+ARKIWCFGP+ TG Sbjct: 587 FMTAEPMSEELSVAIETGHVNPRDDFKVRARIMADEFGWDVTDARKIWCFGPDTTGANVV 646 Query: 182 GGLLQRSSVPQ*N*GLCCGWIPVGPKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQII 361 + + KEG M EENLR RFNI DV LH DAIHRGGGQII Sbjct: 647 VDQTKAVAYLNEIKDSVVAAFAWASKEGPMFEENLRSCRFNILDVVLHADAIHRGGGQII 706 Query: 362 PTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFKSPRWQVHLCS 541 PT RR +YA L A P + EPV+L EIQ E A+GGIY VLN++RGHVF S +V Sbjct: 707 PTARRVVYASTLLASPIIQEPVFLVEIQVSENAMGGIYSVLNKKRGHVF-SEEQRVGTPL 765 Query: 542 L*RPTYLSMSRSV-YCRICVPTPADRPSRSAYFDHWQVLPGEPV 670 YL ++ S + A + FDHW + G+P+ Sbjct: 766 YNIKAYLPVNESFGFTGELRQATAGQAFPQLVFDHWSPMSGDPL 809 Score = 48.0 bits (109), Expect = 2e-06 Identities = 21/36 (58%), Positives = 26/36 (72%) Frame = +3 Query: 156 PRVPGPNILVDCSKGVQYLNEIKDSVVAGFQWALRK 263 P G N++VD +K V YLNEIKDSVVA F WA ++ Sbjct: 638 PDTTGANVVVDQTKAVAYLNEIKDSVVAAFAWASKE 673 Score = 25.4 bits (53), Expect = 9.4 Identities = 10/20 (50%), Positives = 14/20 (70%) Frame = +3 Query: 663 NPCEPQSKPYNVVQETRKRE 722 +P +P SKP +V E RKR+ Sbjct: 807 DPLDPTSKPGQIVCEARKRK 826 >SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elongation factor 2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 842 Score = 188 bits (458), Expect = 8e-49 Identities = 103/224 (45%), Positives = 129/224 (57%), Gaps = 1/224 (0%) Frame = +2 Query: 2 FMKAQPMPDGLPEDIDEGRVNPRDDFKTRARYLTEKYEYDVTEARKIWCFGPEGTGPQHP 181 FM A+PM + L I+ G VNPRDDFK RAR + +++ +DVT+ARKIWCFGP+ TG Sbjct: 587 FMTAEPMSEELSVAIETGHVNPRDDFKVRARIMADEFGWDVTDARKIWCFGPDTTGANVV 646 Query: 182 GGLLQRSSVPQ*N*GLCCGWIPVGPKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQII 361 + + KEG M EENLR RFNI DV LH DAIHRGGGQII Sbjct: 647 VDQTKAVAYLNEIKDSVVAAFAWASKEGPMFEENLRSCRFNILDVVLHADAIHRGGGQII 706 Query: 362 PTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFKSPRWQVHLCS 541 PT RR +YA L A P + EPV+L EIQ E A+GGIY VLN++RGHVF S +V Sbjct: 707 PTARRVVYASTLLASPIIQEPVFLVEIQVSENAMGGIYSVLNKKRGHVF-SEEQRVGTPL 765 Query: 542 L*RPTYLSMSRSV-YCRICVPTPADRPSRSAYFDHWQVLPGEPV 670 YL ++ S + A + FDHW + G+P+ Sbjct: 766 YNIKAYLPVNESFGFTGELRQATAGQAFPQLVFDHWSPMSGDPL 809 Score = 48.0 bits (109), Expect = 2e-06 Identities = 21/36 (58%), Positives = 26/36 (72%) Frame = +3 Query: 156 PRVPGPNILVDCSKGVQYLNEIKDSVVAGFQWALRK 263 P G N++VD +K V YLNEIKDSVVA F WA ++ Sbjct: 638 PDTTGANVVVDQTKAVAYLNEIKDSVVAAFAWASKE 673 Score = 25.4 bits (53), Expect = 9.4 Identities = 10/20 (50%), Positives = 14/20 (70%) Frame = +3 Query: 663 NPCEPQSKPYNVVQETRKRE 722 +P +P SKP +V E RKR+ Sbjct: 807 DPLDPTSKPGQIVCEARKRK 826 >SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces pombe|chr 2|||Manual Length = 983 Score = 135 bits (327), Expect = 6e-33 Identities = 81/232 (34%), Positives = 117/232 (50%), Gaps = 7/232 (3%) Frame = +2 Query: 5 MKAQPMPDGLPEDIDEGRVNPRDDFKTRARYLTEKYEYDVTEARKIWCFGPEGTGPQHPG 184 M +P+ G+ DI+ G+VN K + + + Y++D+ +R IW FGP+ G Sbjct: 700 MVVEPLEKGISNDIENGKVNINWPQKRISEFFQKNYDWDLLASRSIWAFGPDDRGTNILR 759 Query: 185 GLLQRSSVPQ*N*GLCCGWIPVG----PKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGG 352 + V + +I G +EG + +E +R V F + DV L + I+RGGG Sbjct: 760 DDTLSTDVDKNVLNSVKEYIKQGFQWGTREGPLCDETIRNVNFRLMDVVLAPEQIYRGGG 819 Query: 353 QIIPTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFKS---PRW 523 QIIPT RR Y+ LTA PRLMEPVY+ E+ P ++ IY +L RRRGHV + P Sbjct: 820 QIIPTARRVCYSSFLTASPRLMEPVYMVEVHAPADSLPIIYDLLTRRRGHVLQDIPRPGS 879 Query: 524 QVHLCSL*RPTYLSMSRSVYCRICVPTPADRPSRSAYFDHWQVLPGEPVRTS 679 ++L P S R+ A FDHWQV+PG+P+ S Sbjct: 880 PLYLVRALIPVIDSCGFETDLRVHTQGQA---MCQMVFDHWQVVPGDPLDKS 928 >SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 1000 Score = 47.2 bits (107), Expect = 3e-06 Identities = 21/42 (50%), Positives = 25/42 (59%) Frame = +1 Query: 511 ESQVAGTPMFIVKAYLPVNESFGLLPNLRSNTGGQAFPQCVF 636 E GTP FIVKA +PV ESFG + T G A+PQ +F Sbjct: 895 EEMKEGTPFFIVKALIPVVESFGFAVEILKRTSGAAYPQLIF 936 Score = 44.4 bits (100), Expect = 2e-05 Identities = 19/52 (36%), Positives = 30/52 (57%) Frame = +2 Query: 350 GQIIPTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHV 505 GQ+I + + L PRLM +Y C++Q +G +YGV+++RRG V Sbjct: 841 GQVISVVKESIRHGFLGWSPRLMLAMYSCDVQATSEVLGRVYGVVSKRRGRV 892 >SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schizosaccharomyces pombe|chr 2|||Manual Length = 770 Score = 29.9 bits (64), Expect = 0.43 Identities = 20/86 (23%), Positives = 35/86 (40%) Frame = +2 Query: 257 KEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLLTAQPRLMEPVYLC 436 K+G + ++ RF + D H ++ T L A P ++EP+ Sbjct: 624 KKGFLIGHPIKNCRFVLEDGAYHPVDSSELAFRL--ATISAFRTAFLQANPMVLEPIMNV 681 Query: 437 EIQCPEVAVGGIYGVLNRRRGHVFKS 514 I P GG+ G L++R+ + S Sbjct: 682 SITAPVEHQGGVIGNLDKRKATIVDS 707 >SPCC320.12 ||SPCC330.17c|mitochondrial inner membrane peptidase Atp23|Schizosaccharomyces pombe|chr 3|||Manual Length = 185 Score = 27.9 bits (59), Expect = 1.8 Identities = 10/21 (47%), Positives = 14/21 (66%) Frame = -3 Query: 696 RCRACSEVRTGSPGRTCQWSK 634 R +ACSE+R S C+W+K Sbjct: 105 RHQACSEIRASSMSGECRWTK 125 >SPBC19C2.15c ||SPBC2F12.16|phosphoprotein phosphatase |Schizosaccharomyces pombe|chr 2|||Manual Length = 208 Score = 27.1 bits (57), Expect = 3.1 Identities = 11/32 (34%), Positives = 17/32 (53%) Frame = +3 Query: 177 ILVDCSKGVQYLNEIKDSVVAGFQWALRKELW 272 IL G ++L +K + A + W+L K LW Sbjct: 143 ILYPIGAGSEFLLVLKSRIAAQYVWSLNKLLW 174 >SPBC36.07 |iki3||RNA polymerase II elongator subunit Iki3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1253 Score = 26.6 bits (56), Expect = 4.0 Identities = 23/75 (30%), Positives = 29/75 (38%), Gaps = 1/75 (1%) Frame = +2 Query: 32 LPEDIDEGRVNPRDDFKTRARYLTEKYEYDVTEARKIWCFGP-EGTGPQHPGGLLQRSSV 208 LPE IDEG+++ DD KT + + + K GP GLL S Sbjct: 194 LPEKIDEGKLSDVDDGKTYICWRGDSQYVSINRLEK----GPRRAIRVYSREGLLDSISE 249 Query: 209 PQ*N*GLCCGWIPVG 253 PQ W P G Sbjct: 250 PQDGQQSLLSWKPSG 264 >SPAC1006.08 |etd1||ethanol-hypersensitive mutant protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 391 Score = 26.6 bits (56), Expect = 4.0 Identities = 15/49 (30%), Positives = 26/49 (53%), Gaps = 1/49 (2%) Frame = -2 Query: 424 RLHETGL-SS*QTCVQASSSCWNDLATTSMDGISMECYIIDVESNTTQI 281 ++ ET L ++ Q + CW++L+TTS + + II + TT I Sbjct: 191 QIQETSLGATTQEDESSDGICWDELSTTSPESSKVSEPIIQDNTQTTHI 239 >SPBC32F12.09 |rum1||CDK inhibitor Rum1|Schizosaccharomyces pombe|chr 2|||Manual Length = 230 Score = 26.6 bits (56), Expect = 4.0 Identities = 11/26 (42%), Positives = 16/26 (61%) Frame = -2 Query: 160 LGAKAPNLTGFGNIVFVLFCKIPSAS 83 +G PNL GN +F L ++PS+S Sbjct: 169 IGILPPNLNSIGNKMFSLKSRVPSSS 194 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,486,223 Number of Sequences: 5004 Number of extensions: 76948 Number of successful extensions: 211 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 190 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 205 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 383374054 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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