Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30494
(788 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 195 2e-50
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 131 6e-31
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 131 6e-31
At5g25230.1 68418.m02991 elongation factor Tu family protein tra... 124 7e-29
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 44 9e-05
At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 37 0.018
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 35 0.071
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 35 0.071
At5g13650.2 68418.m01585 elongation factor family protein contai... 31 0.87
At5g13650.1 68418.m01584 elongation factor family protein contai... 31 0.87
At5g15580.1 68418.m01824 expressed protein unknown protein F14P3... 30 1.5
At1g01770.1 68414.m00096 expressed protein 29 3.5
At3g02840.1 68416.m00276 immediate-early fungal elicitor family ... 28 8.1
At2g20020.1 68415.m02340 expressed protein 28 8.1
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 195 bits (476), Expect = 2e-50
Identities = 100/232 (43%), Positives = 138/232 (59%), Gaps = 3/232 (1%)
Frame = +2
Query: 2 FMKAQPMPDGLPEDIDEGRVNPRDDFKTRARYLTEKYEYDVTEARKIWCFGPEGTGPQHP 181
+M+A+PM +GL E ID+GR+ PRDD K R++ L E++ +D A+KIW FGPE TGP
Sbjct: 588 YMEARPMEEGLAEAIDDGRIGPRDDPKIRSKILAEEFGWDKDLAKKIWAFGPETTGPNMV 647
Query: 182 GGLLQRSSVPQ*N*GLCCGWIPVGPKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQII 361
+ + KEG +AEEN+RG+ F + DV LH+DAIHRGGGQ+I
Sbjct: 648 VDMCKGVQYLNEIKDSVVAGFQWASKEGPLAEENMRGICFEVCDVVLHSDAIHRGGGQVI 707
Query: 362 PTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFKS---PRWQVH 532
PT RR +YA +TA+PRL+EPVY+ EIQ PE A+GGIY VLN++RGHVF+ P ++
Sbjct: 708 PTARRVIYASQITAKPRLLEPVYMVEIQAPEGALGGIYSVLNQKRGHVFEEMQRPGTPLY 767
Query: 533 LCSL*RPTYLSMSRSVYCRICVPTPADRPSRSAYFDHWQVLPGEPVRTSEQA 688
P S S R + + FDHW+++ +P+ QA
Sbjct: 768 NIKAYLPVVESFGFSSQLRAAT---SGQAFPQCVFDHWEMMSSDPLEPGTQA 816
>At1g06220.2 68414.m00656 elongation factor Tu family protein similar
to Cryptosporidium parvum elongation factor-2 GB:U21667
GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 131 bits (316), Expect = 6e-31
Identities = 84/230 (36%), Positives = 116/230 (50%), Gaps = 8/230 (3%)
Frame = +2
Query: 5 MKAQPMPDGLPEDIDEGRVNPRDDFKTRARYLTEKYEYDVTEARKIWCFGPEGTGPQH-- 178
M A+P+ GL EDI+ G V+ + K + KY++D+ AR IW FGP+ GP
Sbjct: 701 MIAEPLDRGLAEDIENGVVSIDWNRKQLGDFFRTKYDWDLLAARSIWAFGPDKQGPNILL 760
Query: 179 PGGL---LQRSSVPQ*N*GLCCGWIPVGPKEGVMAEENLRGVRFNIYDVTLHTDAIHRGG 349
L + R+ + + G+ G +EG + +E +R V+F I D + + +HRG
Sbjct: 761 DDTLPTEVDRNLMMAVKDSIVQGF-QWGAREGPLCDEPIRNVKFKIVDARIAPEPLHRGS 819
Query: 350 GQIIPTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHV---FKSPR 520
GQ+IPT RR Y+ L A PRLMEPVY EIQ P V IY VL+RRRGHV P
Sbjct: 820 GQMIPTARRVAYSAFLMATPRLMEPVYYVEIQTPIDCVTAIYTVLSRRRGHVTSDVPQPG 879
Query: 521 WQVHLCSL*RPTYLSMSRSVYCRICVPTPADRPSRSAYFDHWQVLPGEPV 670
++ P S R A S FDHW ++PG+P+
Sbjct: 880 TPAYIVKAFLPVIESFGFETDLRYHTQGQAFCLS---VFDHWAIVPGDPL 926
Score = 46.8 bits (106), Expect = 2e-05
Identities = 23/37 (62%), Positives = 27/37 (72%)
Frame = +1
Query: 526 GTPMFIVKAYLPVNESFGLLPNLRSNTGGQAFPQCVF 636
GTP +IVKA+LPV ESFG +LR +T GQAF VF
Sbjct: 879 GTPAYIVKAFLPVIESFGFETDLRYHTQGQAFCLSVF 915
Score = 33.9 bits (74), Expect = 0.12
Identities = 17/43 (39%), Positives = 24/43 (55%), Gaps = 4/43 (9%)
Frame = +3
Query: 147 ALAPRVPGPNILVDCSKGVQY----LNEIKDSVVAGFQWALRK 263
A P GPNIL+D + + + +KDS+V GFQW R+
Sbjct: 748 AFGPDKQGPNILLDDTLPTEVDRNLMMAVKDSIVQGFQWGARE 790
>At1g06220.1 68414.m00655 elongation factor Tu family protein similar
to Cryptosporidium parvum elongation factor-2 GB:U21667
GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 131 bits (316), Expect = 6e-31
Identities = 84/230 (36%), Positives = 116/230 (50%), Gaps = 8/230 (3%)
Frame = +2
Query: 5 MKAQPMPDGLPEDIDEGRVNPRDDFKTRARYLTEKYEYDVTEARKIWCFGPEGTGPQH-- 178
M A+P+ GL EDI+ G V+ + K + KY++D+ AR IW FGP+ GP
Sbjct: 701 MIAEPLDRGLAEDIENGVVSIDWNRKQLGDFFRTKYDWDLLAARSIWAFGPDKQGPNILL 760
Query: 179 PGGL---LQRSSVPQ*N*GLCCGWIPVGPKEGVMAEENLRGVRFNIYDVTLHTDAIHRGG 349
L + R+ + + G+ G +EG + +E +R V+F I D + + +HRG
Sbjct: 761 DDTLPTEVDRNLMMAVKDSIVQGF-QWGAREGPLCDEPIRNVKFKIVDARIAPEPLHRGS 819
Query: 350 GQIIPTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHV---FKSPR 520
GQ+IPT RR Y+ L A PRLMEPVY EIQ P V IY VL+RRRGHV P
Sbjct: 820 GQMIPTARRVAYSAFLMATPRLMEPVYYVEIQTPIDCVTAIYTVLSRRRGHVTSDVPQPG 879
Query: 521 WQVHLCSL*RPTYLSMSRSVYCRICVPTPADRPSRSAYFDHWQVLPGEPV 670
++ P S R A S FDHW ++PG+P+
Sbjct: 880 TPAYIVKAFLPVIESFGFETDLRYHTQGQAFCLS---VFDHWAIVPGDPL 926
Score = 46.8 bits (106), Expect = 2e-05
Identities = 23/37 (62%), Positives = 27/37 (72%)
Frame = +1
Query: 526 GTPMFIVKAYLPVNESFGLLPNLRSNTGGQAFPQCVF 636
GTP +IVKA+LPV ESFG +LR +T GQAF VF
Sbjct: 879 GTPAYIVKAFLPVIESFGFETDLRYHTQGQAFCLSVF 915
Score = 33.9 bits (74), Expect = 0.12
Identities = 17/43 (39%), Positives = 24/43 (55%), Gaps = 4/43 (9%)
Frame = +3
Query: 147 ALAPRVPGPNILVDCSKGVQY----LNEIKDSVVAGFQWALRK 263
A P GPNIL+D + + + +KDS+V GFQW R+
Sbjct: 748 AFGPDKQGPNILLDDTLPTEVDRNLMMAVKDSIVQGFQWGARE 790
>At5g25230.1 68418.m02991 elongation factor Tu family protein
translation Elongation Factor 2, Schizosaccharomyces
pombe, PIR:T39902
Length = 973
Score = 124 bits (299), Expect = 7e-29
Identities = 82/232 (35%), Positives = 113/232 (48%), Gaps = 10/232 (4%)
Frame = +2
Query: 5 MKAQPMPDGLPEDIDEGRVNPRDDFKTRARYLTEKYEYDVTEARKIWCFGPEGTGPQHPG 184
M A+P+ GL EDI+ G V+ + + KY++D+ AR IW FGP+ G
Sbjct: 687 MIAEPLDRGLAEDIENGVVSIDWNRVQLGDFFRTKYDWDLLAARSIWAFGPDKQGTNI-- 744
Query: 185 GLLQRSSVPQ*N*GLCCG-------WIPVGPKEGVMAEENLRGVRFNIYDVTLHTDAIHR 343
LL + + + L G G +EG + +E +R V+F I D + + +HR
Sbjct: 745 -LLDDTLPTEVDRNLMMGVKDSIVQGFQWGAREGPLCDEPIRNVKFKIVDARIAPEPLHR 803
Query: 344 GGGQIIPTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHV---FKS 514
G GQ+IPT RR Y+ L A PRLMEPVY EIQ P V IY VL+RRRG+V
Sbjct: 804 GSGQMIPTARRVAYSAFLMATPRLMEPVYYVEIQTPIDCVTAIYTVLSRRRGYVTSDVPQ 863
Query: 515 PRWQVHLCSL*RPTYLSMSRSVYCRICVPTPADRPSRSAYFDHWQVLPGEPV 670
P ++ P S R A S FDHW ++PG+P+
Sbjct: 864 PGTPAYIVKAFLPVIESFGFETDLRYHTQGQAFCLS---VFDHWAIVPGDPL 912
Score = 46.8 bits (106), Expect = 2e-05
Identities = 23/37 (62%), Positives = 27/37 (72%)
Frame = +1
Query: 526 GTPMFIVKAYLPVNESFGLLPNLRSNTGGQAFPQCVF 636
GTP +IVKA+LPV ESFG +LR +T GQAF VF
Sbjct: 865 GTPAYIVKAFLPVIESFGFETDLRYHTQGQAFCLSVF 901
Score = 30.3 bits (65), Expect = 1.5
Identities = 16/43 (37%), Positives = 23/43 (53%), Gaps = 4/43 (9%)
Frame = +3
Query: 147 ALAPRVPGPNILVDCSKGVQY----LNEIKDSVVAGFQWALRK 263
A P G NIL+D + + + +KDS+V GFQW R+
Sbjct: 734 AFGPDKQGTNILLDDTLPTEVDRNLMMGVKDSIVQGFQWGARE 776
>At3g22980.1 68416.m02898 elongation factor Tu family protein similar
to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 44.4 bits (100), Expect = 9e-05
Identities = 18/54 (33%), Positives = 30/54 (55%)
Frame = +2
Query: 350 GQIIPTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFK 511
GQ++ + A +L PR++E +Y CE+ +G +Y VL+RRR + K
Sbjct: 853 GQVMTAVKDACRAAVLQTNPRIVEAMYFCELNTAPEYLGPMYAVLSRRRARILK 906
Score = 37.9 bits (84), Expect = 0.008
Identities = 17/37 (45%), Positives = 22/37 (59%)
Frame = +1
Query: 508 QESQVAGTPMFIVKAYLPVNESFGLLPNLRSNTGGQA 618
+E G+ +F V AY+PV+ESFG LR T G A
Sbjct: 906 KEEMQEGSSLFTVHAYVPVSESFGFADELRKGTSGGA 942
>At1g62750.1 68414.m07082 elongation factor Tu family protein
similar to elongation factor G SP:P34811 [Glycine max
(Soybean)]
Length = 783
Score = 36.7 bits (81), Expect = 0.018
Identities = 15/35 (42%), Positives = 22/35 (62%)
Frame = +2
Query: 401 AQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHV 505
A PR++EP+ E+ PE +G + G LN RRG +
Sbjct: 683 AGPRMLEPIMRVEVVTPEEHLGDVIGDLNSRRGQI 717
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 34.7 bits (76), Expect = 0.071
Identities = 14/37 (37%), Positives = 23/37 (62%)
Frame = +2
Query: 389 CLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRG 499
C A+P ++EPV L E++ P G + G +N+R+G
Sbjct: 649 CYTAARPVILEPVMLVELKVPTEFQGTVAGDINKRKG 685
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 34.7 bits (76), Expect = 0.071
Identities = 14/37 (37%), Positives = 23/37 (62%)
Frame = +2
Query: 389 CLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRG 499
C A+P ++EPV L E++ P G + G +N+R+G
Sbjct: 649 CYTAARPVILEPVMLVELKVPTEFQGTVAGDINKRKG 685
>At5g13650.2 68418.m01585 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 676
Score = 31.1 bits (67), Expect = 0.87
Identities = 11/33 (33%), Positives = 21/33 (63%)
Frame = +2
Query: 410 RLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVF 508
+L+EP + ++ PE +G + +L +RRG +F
Sbjct: 473 KLLEPYEIATVEVPEAHMGPVVELLGKRRGQMF 505
>At5g13650.1 68418.m01584 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 675
Score = 31.1 bits (67), Expect = 0.87
Identities = 11/33 (33%), Positives = 21/33 (63%)
Frame = +2
Query: 410 RLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVF 508
+L+EP + ++ PE +G + +L +RRG +F
Sbjct: 472 KLLEPYEIATVEVPEAHMGPVVELLGKRRGQMF 504
>At5g15580.1 68418.m01824 expressed protein unknown protein F14P3.18
- Arabidopsis thaliana, EMBL:AC009755
Length = 927
Score = 30.3 bits (65), Expect = 1.5
Identities = 19/45 (42%), Positives = 25/45 (55%)
Frame = +1
Query: 58 RESPR*LQDSRSVSYRKVRIRCYRSP*DLVLWPRGYRAPTSWWIA 192
+E+PR DSRS S+R R C P +LV G+R TS +A
Sbjct: 223 KETPRLSLDSRSNSFRSARSSCSPEPQELV---TGHRRTTSSVVA 264
>At1g01770.1 68414.m00096 expressed protein
Length = 632
Score = 29.1 bits (62), Expect = 3.5
Identities = 17/57 (29%), Positives = 25/57 (43%)
Frame = -1
Query: 749 SLGDLPLNLFPLSRFLYNVVGLALRFARVLQGGPANGRNTHCGKACPPVLERKFGSK 579
S GD+ L + L + + V L F + GPA G G VLE++ S+
Sbjct: 401 SCGDIRLRMDGLFKLKEHAVQLTKEFTALYTNGPAGGGGISTGHKMEIVLEKRLVSR 457
>At3g02840.1 68416.m00276 immediate-early fungal elicitor family
protein similar to immediate-early fungal elicitor
protein CMPG1 (GI:14582200) [Petroselinum crispum]
Length = 379
Score = 27.9 bits (59), Expect = 8.1
Identities = 18/66 (27%), Positives = 32/66 (48%)
Frame = +1
Query: 478 CAEQTSWSRFQESQVAGTPMFIVKAYLPVNESFGLLPNLRSNTGGQAFPQCVFRPLAGPP 657
C E+ + +R ++ +F++ ++LP+N S G + G A C+ R L G
Sbjct: 107 CFERFAAARDGHMRLLEEILFVLSSWLPLNRSEGF-----NKMGSTASLNCLVRFLNGKD 161
Query: 658 WRTRAN 675
+TR N
Sbjct: 162 AKTRQN 167
>At2g20020.1 68415.m02340 expressed protein
Length = 701
Score = 27.9 bits (59), Expect = 8.1
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = -3
Query: 294 TPRKFSSAITPSLGPTGIQPQQSP 223
TPR+F S P +G G++P Q P
Sbjct: 197 TPREFDSFRLPPVGKKGLKPVQKP 220
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,702,392
Number of Sequences: 28952
Number of extensions: 433609
Number of successful extensions: 1110
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 1054
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1105
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1775300800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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