BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30493 (752 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 31 0.18 SPCC1020.04c |rpb6|rpo15|DNA-directed RNA polymerase I, II and I... 31 0.23 SPBC354.13 |rga6||GTPase activating protein Rga6|Schizosaccharom... 29 0.54 SPAC343.09 |ubx3|mug39|UBX domain protein Ubx3|Schizosaccharomyc... 29 0.94 SPCC645.14c |sti1||chaperone activator Sti1 |Schizosaccharomyces... 28 1.2 SPBC428.02c |eca39|SPBC582.12c|branched chain amino acid aminotr... 28 1.6 SPAC4F10.13c |mpd2||GYF domain|Schizosaccharomyces pombe|chr 1||... 28 1.6 SPAC8C9.04 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||... 27 3.8 SPCC622.12c |||NADP-specific glutamate dehydrogenase |Schizosacc... 27 3.8 SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 27 3.8 SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 27 3.8 SPBC19C7.10 |||transcription factor |Schizosaccharomyces pombe|c... 26 5.0 SPBC1709.15c |cft2||cleavage factor two Cft2/polyadenylation fac... 26 6.6 SPBC1289.04c |pob1||Boi family protein|Schizosaccharomyces pombe... 25 8.8 SPCC285.17 |spp27|uaf30|RNA polymerase I upstream activation fac... 25 8.8 SPBC16A3.19 |||histone acetyltransferase complex subunit Eaf7 |S... 25 8.8 SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr 2... 25 8.8 SPCC5E4.05c |||serine hydrolase |Schizosaccharomyces pombe|chr 3... 25 8.8 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 31.1 bits (67), Expect = 0.18 Identities = 16/45 (35%), Positives = 19/45 (42%) Frame = +2 Query: 275 PNRPGAQSTEKPAEATSTTGVASAAPDIAKPSESNPPTETKPEQP 409 P GA KP+ A V S AP + KPS + PP P Sbjct: 1115 PAPSGAPPVPKPSVAAPPVPVPSGAPPVPKPSVAAPPVPAPSGAP 1159 Score = 28.7 bits (61), Expect = 0.94 Identities = 14/37 (37%), Positives = 17/37 (45%) Frame = +2 Query: 275 PNRPGAQSTEKPAEATSTTGVASAAPDIAKPSESNPP 385 P GA KP+ A S AP + KPS + PP Sbjct: 1134 PVPSGAPPVPKPSVAAPPVPAPSGAPPVPKPSVAAPP 1170 Score = 27.1 bits (57), Expect = 2.9 Identities = 18/61 (29%), Positives = 26/61 (42%) Frame = +2 Query: 290 AQSTEKPAEATSTTGVASAAPDIAKPSESNPPTETKPEQPAAGPLKQIFENSPVLQGIAG 469 A KP+ A S AP + KPS + PP P A P+ + +P + +G Sbjct: 1101 APPVPKPSVAVPPVPAPSGAPPVPKPSVAAPPV---PVPSGAPPVPKPSVAAPPVPAPSG 1157 Query: 470 A 472 A Sbjct: 1158 A 1158 >SPCC1020.04c |rpb6|rpo15|DNA-directed RNA polymerase I, II and III subunit Rpb6|Schizosaccharomyces pombe|chr 3|||Manual Length = 142 Score = 30.7 bits (66), Expect = 0.23 Identities = 22/89 (24%), Positives = 39/89 (43%), Gaps = 2/89 (2%) Frame = +2 Query: 278 NRPGAQSTEKPAEATSTTGVASAAPDIAKPSESNPPTETKPEQPAAGPLK--QIFENSPV 451 + PG ST E +++ A + +AK + P TK E+ + QI N+PV Sbjct: 38 SHPGEPSTTVITEDVASSKTAQSGKAVAKEDRTTTPYMTKYERARILGTRALQISMNAPV 97 Query: 452 LQGIAGAVKKIQTTVNNPVSQEILKWLKR 538 L + G +Q + ++I ++R Sbjct: 98 LVDLEGETDPLQIAMKELAQKKIPLLVRR 126 >SPBC354.13 |rga6||GTPase activating protein Rga6|Schizosaccharomyces pombe|chr 2|||Manual Length = 733 Score = 29.5 bits (63), Expect = 0.54 Identities = 21/88 (23%), Positives = 32/88 (36%) Frame = +2 Query: 272 QPNRPGAQSTEKPAEATSTTGVASAAPDIAKPSESNPPTETKPEQPAAGPLKQIFENSPV 451 QP + ++ + S S+ I+ ++S P T T P P K I SPV Sbjct: 636 QPQPKISTVSDTAVPSMSFANNISSRSVISAATDSKPSTRTSP--PFVNNTKPIVAKSPV 693 Query: 452 LQGIAGAVKKIQTTVNNPVSQEILKWLK 535 + K +N S + W K Sbjct: 694 TVTASSETNKKSQKINKKASPRVSLWTK 721 >SPAC343.09 |ubx3|mug39|UBX domain protein Ubx3|Schizosaccharomyces pombe|chr 1|||Manual Length = 410 Score = 28.7 bits (61), Expect = 0.94 Identities = 18/37 (48%), Positives = 22/37 (59%) Frame = +2 Query: 299 TEKPAEATSTTGVASAAPDIAKPSESNPPTETKPEQP 409 TE A STT V + P P E NPPTE++PE+P Sbjct: 181 TENEASG-STTPVTQSGP----PRE-NPPTESQPEKP 211 >SPCC645.14c |sti1||chaperone activator Sti1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 591 Score = 28.3 bits (60), Expect = 1.2 Identities = 14/40 (35%), Positives = 21/40 (52%) Frame = +2 Query: 290 AQSTEKPAEATSTTGVASAAPDIAKPSESNPPTETKPEQP 409 A+ EK E ++ SA D AKP +NPP + + +P Sbjct: 205 AEEQEKKEEFAPSSSTPSA--DSAKPETTNPPPQPQASEP 242 >SPBC428.02c |eca39|SPBC582.12c|branched chain amino acid aminotransferase Eca39|Schizosaccharomyces pombe|chr 2|||Manual Length = 380 Score = 27.9 bits (59), Expect = 1.6 Identities = 11/28 (39%), Positives = 15/28 (53%), Gaps = 2/28 (7%) Frame = +1 Query: 7 EWNSNQAWSVPNI--FGGVALQPLSQMF 84 +WN + WS P I FG + P S +F Sbjct: 49 KWNREKGWSTPEIVPFGKLCFHPASSVF 76 >SPAC4F10.13c |mpd2||GYF domain|Schizosaccharomyces pombe|chr 1|||Manual Length = 992 Score = 27.9 bits (59), Expect = 1.6 Identities = 10/29 (34%), Positives = 18/29 (62%) Frame = +3 Query: 51 RSSTAAPQSDVQGDATTTTQRPAPWQNLP 137 +S A+ + V + TT +P+PW++LP Sbjct: 603 QSPAASKEIPVTSGSQTTAPKPSPWKSLP 631 >SPAC8C9.04 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 647 Score = 26.6 bits (56), Expect = 3.8 Identities = 22/73 (30%), Positives = 29/73 (39%) Frame = +2 Query: 275 PNRPGAQSTEKPAEATSTTGVASAAPDIAKPSESNPPTETKPEQPAAGPLKQIFENSPVL 454 P R Q PA TS SAA +K E+ ++P A K+I E P+ Sbjct: 187 PQRNDEQLGNSPASITSKPATTSAAQPSSKVEENMAKATSQPITTAE---KEIPELKPIE 243 Query: 455 QGIAGAVKKIQTT 493 K+I TT Sbjct: 244 PEAIMISKEINTT 256 >SPCC622.12c |||NADP-specific glutamate dehydrogenase |Schizosaccharomyces pombe|chr 3|||Manual Length = 451 Score = 26.6 bits (56), Expect = 3.8 Identities = 13/46 (28%), Positives = 26/46 (56%) Frame = +2 Query: 308 PAEATSTTGVASAAPDIAKPSESNPPTETKPEQPAAGPLKQIFENS 445 P +A + GVA + ++A+ ++ + T + + AG ++ IFE S Sbjct: 365 PGKAANAGGVAVSGLEMAQNAQFSTWTHAEVDAKLAGIMQNIFEQS 410 >SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1224 Score = 26.6 bits (56), Expect = 3.8 Identities = 15/50 (30%), Positives = 20/50 (40%) Frame = +2 Query: 266 LLQPNRPGAQSTEKPAEATSTTGVASAAPDIAKPSESNPPTETKPEQPAA 415 + P P T P A+ T P + +PS NP T P+ P A Sbjct: 923 IASPPPPKVGETYHPPTASGTR-----VPPVQQPSHPNPYTPVAPQSPVA 967 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 26.6 bits (56), Expect = 3.8 Identities = 10/20 (50%), Positives = 12/20 (60%) Frame = +2 Query: 341 SAAPDIAKPSESNPPTETKP 400 SA+P +A SNPP E P Sbjct: 522 SASPSVASTKTSNPPVEAPP 541 >SPBC19C7.10 |||transcription factor |Schizosaccharomyces pombe|chr 2|||Manual Length = 432 Score = 26.2 bits (55), Expect = 5.0 Identities = 23/87 (26%), Positives = 38/87 (43%), Gaps = 1/87 (1%) Frame = +2 Query: 287 GAQSTEKPAEATSTTGVASAAPDIAKPSESNPPTETKPEQPAAGPLKQIFENSPVLQG-I 463 G STEK + +S+ A + +PSE T ++ P+K P L G I Sbjct: 229 GGSSTEKVGQPSSSDDKAKGSTSKDQPSEEEEKTSDIQDRKIKTPIK------PSLLGKI 282 Query: 464 AGAVKKIQTTVNNPVSQEILKWLKRLN 544 +V K T V + V++ + ++N Sbjct: 283 RSSVNKGMTDVASQVNRGMTDVASQVN 309 >SPBC1709.15c |cft2||cleavage factor two Cft2/polyadenylation factor CPSF-73 |Schizosaccharomyces pombe|chr 2|||Manual Length = 797 Score = 25.8 bits (54), Expect = 6.6 Identities = 11/23 (47%), Positives = 13/23 (56%) Frame = +1 Query: 514 RDSEVVEETKSEQEKGGVILLPV 582 RD +E S KGG +LLPV Sbjct: 207 RDEAFIESVMSSLLKGGTVLLPV 229 >SPBC1289.04c |pob1||Boi family protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 871 Score = 25.4 bits (53), Expect = 8.8 Identities = 12/35 (34%), Positives = 17/35 (48%) Frame = +2 Query: 341 SAAPDIAKPSESNPPTETKPEQPAAGPLKQIFENS 445 SA P+ +SN P+ T P + P I +NS Sbjct: 415 SAVPENVSRFDSNEPSATSPILKRSSPTDSISQNS 449 >SPCC285.17 |spp27|uaf30|RNA polymerase I upstream activation factor complex subunit Spp27|Schizosaccharomyces pombe|chr 3|||Manual Length = 233 Score = 25.4 bits (53), Expect = 8.8 Identities = 14/35 (40%), Positives = 21/35 (60%), Gaps = 6/35 (17%) Frame = +3 Query: 645 VEKRKPARKSKGI------PPEGKKQGRRVKRGQE 731 +EK+ PARK+KG EGKK +R ++ +E Sbjct: 64 LEKQAPARKTKGKRATENGTEEGKKPAKRTRKRKE 98 >SPBC16A3.19 |||histone acetyltransferase complex subunit Eaf7 |Schizosaccharomyces pombe|chr 2|||Manual Length = 272 Score = 25.4 bits (53), Expect = 8.8 Identities = 13/51 (25%), Positives = 23/51 (45%) Frame = +2 Query: 269 LQPNRPGAQSTEKPAEATSTTGVASAAPDIAKPSESNPPTETKPEQPAAGP 421 +QP P + + E++ T S +P + S T+P +PA+ P Sbjct: 204 IQPVIPEKKEKSEKKESSMTLRKRSVSPSSQNTARSPKRMATEPIEPASSP 254 >SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr 2|||Manual Length = 1217 Score = 25.4 bits (53), Expect = 8.8 Identities = 15/51 (29%), Positives = 21/51 (41%) Frame = +2 Query: 263 NLLQPNRPGAQSTEKPAEATSTTGVASAAPDIAKPSESNPPTETKPEQPAA 415 N Q P +T +AT T + AP + S+P +KP P A Sbjct: 1036 NTTQAAAPVTSTTTTIKQAT-TVSASKPAPSTVTSAASSPSNISKPSAPVA 1085 Score = 25.4 bits (53), Expect = 8.8 Identities = 16/42 (38%), Positives = 22/42 (52%) Frame = +2 Query: 296 STEKPAEATSTTGVASAAPDIAKPSESNPPTETKPEQPAAGP 421 S KPA +T T AS+ +I+KPS P +P+A P Sbjct: 1058 SASKPAPST-VTSAASSPSNISKPSA---PVANNVSKPSAVP 1095 >SPCC5E4.05c |||serine hydrolase |Schizosaccharomyces pombe|chr 3|||Manual Length = 378 Score = 25.4 bits (53), Expect = 8.8 Identities = 19/60 (31%), Positives = 27/60 (45%), Gaps = 2/60 (3%) Frame = +2 Query: 311 AEATSTTGVASAAPDIAKPSESNPPTETKPEQ-PA-AGPLKQIFENSPVLQGIAGAVKKI 484 A + S TGV S++ PP E KPE PA A E++ V + I + K+ Sbjct: 299 APSASPTGVPVEEESHKATSDAVPPAEAKPEPVPASAAERAPTSESTTVPETIVASTTKV 358 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,125,384 Number of Sequences: 5004 Number of extensions: 66190 Number of successful extensions: 249 Number of sequences better than 10.0: 18 Number of HSP's better than 10.0 without gapping: 222 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 249 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 359287726 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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