BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30490 (847 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g24500.2 68417.m03513 hydroxyproline-rich glycoprotein family... 30 1.7 At4g24500.1 68417.m03512 hydroxyproline-rich glycoprotein family... 30 1.7 At5g04170.1 68418.m00405 calcium-binding EF hand family protein ... 29 3.9 At3g29030.1 68416.m03627 expansin, putative (EXP5) identical to ... 28 6.8 At4g14620.1 68417.m02250 expressed protein contains Pfam profile... 28 9.0 At3g42950.1 68416.m04511 glycoside hydrolase family 28 protein /... 28 9.0 At3g17350.1 68416.m02217 expressed protein 28 9.0 >At4g24500.2 68417.m03513 hydroxyproline-rich glycoprotein family protein Length = 290 Score = 30.3 bits (65), Expect = 1.7 Identities = 12/24 (50%), Positives = 13/24 (54%) Frame = -2 Query: 738 WNRKARPGPV*YHGVRLWVPNPPP 667 WN RP PV + G WVP P P Sbjct: 115 WNNNFRPPPVNHSGPPQWVPRPFP 138 >At4g24500.1 68417.m03512 hydroxyproline-rich glycoprotein family protein Length = 319 Score = 30.3 bits (65), Expect = 1.7 Identities = 12/24 (50%), Positives = 13/24 (54%) Frame = -2 Query: 738 WNRKARPGPV*YHGVRLWVPNPPP 667 WN RP PV + G WVP P P Sbjct: 144 WNNNFRPPPVNHSGPPQWVPRPFP 167 >At5g04170.1 68418.m00405 calcium-binding EF hand family protein low similarity to peflin [Homo sapiens] GI:6015440; contains INTERPRO:IPR002048 calcium-binding EF-hand domain Length = 354 Score = 29.1 bits (62), Expect = 3.9 Identities = 21/59 (35%), Positives = 29/59 (49%), Gaps = 1/59 (1%) Frame = -1 Query: 838 LPGYPKAVKG-GWGHPPG*NQELRPQVPTGNPGAMEPQSQAWTGVIPWGPPVGAQSSPR 665 + GYP +G G+G+ G NQ PQ P + G P + T P+ P GA S P+ Sbjct: 1 MSGYPPTSQGYGYGYGGG-NQPPPPQPPYSSGGNNPPYGSSTTSS-PYAVPYGA-SKPQ 56 >At3g29030.1 68416.m03627 expansin, putative (EXP5) identical to expansin At-EXP5 GB:AAB38071 from [Arabidopsis thaliana]; alpha-expansin gene family, PMID:11641069 Length = 255 Score = 28.3 bits (60), Expect = 6.8 Identities = 14/30 (46%), Positives = 15/30 (50%), Gaps = 2/30 (6%) Frame = -3 Query: 767 PGPNR*PW--GHGTAKPGLDRCNTMGSACG 684 PG + PW H T G D TMG ACG Sbjct: 31 PGGHMGPWINAHATFYGGGDASGTMGGACG 60 >At4g14620.1 68417.m02250 expressed protein contains Pfam profile PF04720: Protein of unknown function (DUF506) Length = 341 Score = 27.9 bits (59), Expect = 9.0 Identities = 10/21 (47%), Positives = 14/21 (66%) Frame = +1 Query: 55 SYKKKGIHFRKWKPSETMECK 117 S KKKG+HF W+ ++ M K Sbjct: 239 SLKKKGMHFPPWRKADYMRAK 259 >At3g42950.1 68416.m04511 glycoside hydrolase family 28 protein / polygalacturonase (pectinase) family protein weak similarity to polygalacturonase precursor [Cucumis melo] GI:3320460; contains PF00295: Glycosyl hydrolases family 28 Length = 484 Score = 27.9 bits (59), Expect = 9.0 Identities = 18/49 (36%), Positives = 20/49 (40%), Gaps = 5/49 (10%) Frame = -1 Query: 847 WVFLPGYPKAVKGGWGHPPG*N-----QELRPQVPTGNPGAMEPQSQAW 716 W LP P G H P Q LR V TGN G++ Q Q W Sbjct: 140 WSLLPPLPSYGYGREHHGPRYGSFIHGQNLRDVVVTGNNGSINGQGQTW 188 >At3g17350.1 68416.m02217 expressed protein Length = 278 Score = 27.9 bits (59), Expect = 9.0 Identities = 17/53 (32%), Positives = 22/53 (41%) Frame = -3 Query: 842 FFTRLPQGRKRRVGPSSWVKPGIEAPGPNR*PWGHGTAKPGLDRCNTMGSACG 684 + T + G+ R VGP W GIE + + G DRC G CG Sbjct: 202 YTTVIDNGKMRGVGPLDW-SYGIEL--------SYSVTEIGCDRCRKSGGTCG 245 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 19,079,729 Number of Sequences: 28952 Number of extensions: 434074 Number of successful extensions: 891 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 842 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 890 length of database: 12,070,560 effective HSP length: 80 effective length of database: 9,754,400 effective search space used: 1960634400 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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