Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS30486
         (822 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g62940.1 68414.m07107 4-coumarate--CoA ligase family protein ...    33   0.17 
At3g21230.1 68416.m02683 4-coumarate--CoA ligase, putative / 4-c...    32   0.53 
At1g65060.2 68414.m07376 4-coumarate--CoA ligase 3 / 4-coumaroyl...    28   8.6  
At1g65060.1 68414.m07375 4-coumarate--CoA ligase 3 / 4-coumaroyl...    28   8.6  

>At1g62940.1 68414.m07107 4-coumarate--CoA ligase family protein /
           4-coumaroyl-CoA synthase family protein similar to
           gi:112801 from Petroselinum crispum, GB:AAD40664 from
           [Solanum tuberosum] (J. Biol. Chem. 266 (13), 8551-8559
           (1991)); contains Pfam AMP-binding enzyme domain PF00501
          Length = 542

 Score = 33.5 bits (73), Expect = 0.17
 Identities = 15/43 (34%), Positives = 27/43 (62%)
 Frame = +3

Query: 126 DSVCQIDAATGETETNASVLQRSVRLAKYMRTLGLNPGDVLAL 254
           ++V  ++A TG+  T   V++ + RLAK + +LGL  G V+ +
Sbjct: 41  ENVAFVEAVTGKAVTYGDVVRDTKRLAKALTSLGLRKGQVMVV 83


>At3g21230.1 68416.m02683 4-coumarate--CoA ligase, putative /
           4-coumaroyl-CoA synthase, putative (4CL) similar to 4CL2
           [gi:12229665] and 4CL1 [gi:12229649] from [Arabidopsis
           thaliana], 4CL1 [gi:12229631] from Nicotiana tabacum
          Length = 570

 Score = 31.9 bits (69), Expect = 0.53
 Identities = 17/42 (40%), Positives = 22/42 (52%)
 Frame = +3

Query: 129 SVCQIDAATGETETNASVLQRSVRLAKYMRTLGLNPGDVLAL 254
           + C ID ATG   T A V     R+A  +  LG+  GDV+ L
Sbjct: 62  TTCIIDGATGRILTYADVQTNMRRIAAGIHRLGIRHGDVVML 103


>At1g65060.2 68414.m07376 4-coumarate--CoA ligase 3 /
           4-coumaroyl-CoA synthase 3 (4CL3) identical to SP|Q9S777
           4-coumarate--CoA ligase 3 (EC 6.2.1.12) (4CL 3)
           (4-coumaroyl-CoA synthase 3) {Arabidopsis thaliana}
          Length = 495

 Score = 27.9 bits (59), Expect = 8.6
 Identities = 19/78 (24%), Positives = 36/78 (46%), Gaps = 1/78 (1%)
 Frame = +3

Query: 63  NIDDPQYHMGHLF-MDCMRRRPDSVCQIDAATGETETNASVLQRSVRLAKYMRTLGLNPG 239
           +ID P +   H +  + +    D  C I  +TG++ T         R+A  +  LG+  G
Sbjct: 41  DIDIPNHLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGLYKLGIRKG 100

Query: 240 DVLALGEETTWIFIYHIM 293
           DV+ +  + +  F++  M
Sbjct: 101 DVIMILLQNSAEFVFSFM 118


>At1g65060.1 68414.m07375 4-coumarate--CoA ligase 3 /
           4-coumaroyl-CoA synthase 3 (4CL3) identical to SP|Q9S777
           4-coumarate--CoA ligase 3 (EC 6.2.1.12) (4CL 3)
           (4-coumaroyl-CoA synthase 3) {Arabidopsis thaliana}
          Length = 561

 Score = 27.9 bits (59), Expect = 8.6
 Identities = 19/78 (24%), Positives = 36/78 (46%), Gaps = 1/78 (1%)
 Frame = +3

Query: 63  NIDDPQYHMGHLF-MDCMRRRPDSVCQIDAATGETETNASVLQRSVRLAKYMRTLGLNPG 239
           +ID P +   H +  + +    D  C I  +TG++ T         R+A  +  LG+  G
Sbjct: 41  DIDIPNHLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGLYKLGIRKG 100

Query: 240 DVLALGEETTWIFIYHIM 293
           DV+ +  + +  F++  M
Sbjct: 101 DVIMILLQNSAEFVFSFM 118


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,377,914
Number of Sequences: 28952
Number of extensions: 350371
Number of successful extensions: 882
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 864
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 882
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1882599200
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)