BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30485 (628 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC132.02 |hst2||Sir2 family histone deacetylase Hst2|Schizosac... 63 3e-11 SPBC16D10.07c |sir2||Sir2 family histone deacetylase Sir2|Schizo... 36 0.005 SPCC645.11c |mug117||meiotically upregulated gene Mug117|Schizos... 26 3.9 SPAC13A11.06 ||SPAC3H8.01|pyruvate decarboxylase |Schizosaccharo... 26 5.1 SPBC18H10.15 |ppk23||serine/threonine protein kinase Ppk23|Schiz... 25 6.8 SPAC821.09 |eng1||endo-1,3-beta-glucanase Eng1|Schizosaccharomyc... 25 9.0 SPAC23D3.04c |gpd2||glycerol-3-phosphate dehydrogenase Gpd2|Schi... 25 9.0 >SPCC132.02 |hst2||Sir2 family histone deacetylase Hst2|Schizosaccharomyces pombe|chr 3|||Manual Length = 332 Score = 63.3 bits (147), Expect = 3e-11 Identities = 48/143 (33%), Positives = 63/143 (44%), Gaps = 2/143 (1%) Frame = +1 Query: 16 TAGHFAIAKFEDNHGSQKKITVITQNVDGLHARAGT--KRLIELHGNLYKTRCTKCKEVL 189 T H+ I D QK TQN+D L AG K LIE HG+ +RC +C E+ Sbjct: 97 TYTHYFIRLLHDKRLLQK---CYTQNIDTLERLAGVPDKALIEAHGSFQYSRCIECYEMA 153 Query: 190 VNNDSPICTCK*RSPRF*YGGFDIPVKLLPHCKKAHCGALLRPHIVWFGESLEHDILEAA 369 C I K +P C C L++P IV++GE L E Sbjct: 154 ETEYVRAC---------------IMQKQVPKCNS--CKGLIKPMIVFYGEGLPMRFFEHM 196 Query: 370 EHAMSTCDVCLVVGTSSVVYPAA 438 E CD+ LV+GTS +V+P A Sbjct: 197 EKDTKVCDMALVIGTSLLVHPFA 219 >SPBC16D10.07c |sir2||Sir2 family histone deacetylase Sir2|Schizosaccharomyces pombe|chr 2|||Manual Length = 475 Score = 35.9 bits (79), Expect = 0.005 Identities = 19/59 (32%), Positives = 29/59 (49%), Gaps = 2/59 (3%) Frame = +1 Query: 10 NYTAGHFAIAKFEDNHGSQKKITVITQNVDGLHARAGTK--RLIELHGNLYKTRCTKCK 180 +Y+ H I E + K T+ TQN+D L + G ++I+ HG+ C KCK Sbjct: 223 HYSPSHAFIRLLEKKN---KLSTLFTQNIDNLEKKTGLSDNKIIQCHGSFATATCIKCK 278 >SPCC645.11c |mug117||meiotically upregulated gene Mug117|Schizosaccharomyces pombe|chr 3|||Manual Length = 186 Score = 26.2 bits (55), Expect = 3.9 Identities = 14/33 (42%), Positives = 19/33 (57%), Gaps = 3/33 (9%) Frame = -2 Query: 555 QC--CTTRSFEVKNGNQVGRCWFYIKFCY-NCT 466 QC C TR F N N+ RC+ + +CY +CT Sbjct: 138 QCSACGTRFFN--NDNENTRCYVKLNYCYPDCT 168 >SPAC13A11.06 ||SPAC3H8.01|pyruvate decarboxylase |Schizosaccharomyces pombe|chr 1|||Manual Length = 571 Score = 25.8 bits (54), Expect = 5.1 Identities = 12/41 (29%), Positives = 18/41 (43%) Frame = -2 Query: 573 SASACGQCCTTRSFEVKNGNQVGRCWFYIKFCYNCTPRSCL 451 +A G + E + N++ CWF+ KF PR L Sbjct: 349 AAPTIGYNIKPKHAEGYSSNEITHCWFWPKFSEFLKPRDVL 389 >SPBC18H10.15 |ppk23||serine/threonine protein kinase Ppk23|Schizosaccharomyces pombe|chr 2|||Manual Length = 398 Score = 25.4 bits (53), Expect = 6.8 Identities = 13/40 (32%), Positives = 19/40 (47%), Gaps = 4/40 (10%) Frame = -1 Query: 448 EQTLPQDIPLMMYQQLG----IHHRWTLHVQLLPKYHVLN 341 E L ++ +M Q L +HH W LH L P ++N Sbjct: 168 EDFLQSEVKTLMLQLLAATAFMHHHWYLHRDLKPSNLLMN 207 >SPAC821.09 |eng1||endo-1,3-beta-glucanase Eng1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1016 Score = 25.0 bits (52), Expect = 9.0 Identities = 8/16 (50%), Positives = 11/16 (68%) Frame = +3 Query: 426 ISCGNVCSTSSFEGCN 473 ++CGN C SS GC+ Sbjct: 994 LACGNACYDSSIYGCS 1009 >SPAC23D3.04c |gpd2||glycerol-3-phosphate dehydrogenase Gpd2|Schizosaccharomyces pombe|chr 1|||Manual Length = 373 Score = 25.0 bits (52), Expect = 9.0 Identities = 11/35 (31%), Positives = 19/35 (54%) Frame = +2 Query: 146 EIYTKLDVQNVRKYW*IMTALYALANRGAPDSNMV 250 E+Y LD QN + + TA+Y + G P + ++ Sbjct: 336 EVYEFLDNQNKVSEFPLFTAVYRIVYEGLPPNKLL 370 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,940,488 Number of Sequences: 5004 Number of extensions: 65623 Number of successful extensions: 154 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 148 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 154 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 277683324 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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