Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS30473
         (702 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g57470.1 68416.m06398 peptidase M16 family protein / insulina...    62   3e-10
At1g06900.1 68414.m00733 peptidase M16 family protein / insulina...    60   2e-09
At2g41790.1 68415.m05165 peptidase M16 family protein / insulina...    56   2e-08
At3g57460.1 68416.m06397 expressed protein weak similarity to SP...    43   2e-04
At5g51300.2 68418.m06360 splicing factor-related contains simila...    29   3.0  
At5g51300.1 68418.m06359 splicing factor-related contains simila...    29   3.0  
At3g11590.1 68416.m01416 expressed protein                             29   3.9  
At1g11540.1 68414.m01325 expressed protein  contains Pfam profil...    29   3.9  
At2g17000.1 68415.m01960 mechanosensitive ion channel domain-con...    28   5.2  
At5g04230.1 68418.m00412 phenylalanine ammonia-lyase 3 (PAL3) ne...    27   9.1  
At1g80510.1 68414.m09435 amino acid transporter family protein s...    27   9.1  

>At3g57470.1 68416.m06398 peptidase M16 family protein / insulinase
           family protein contains weak similarity to Pfam domain,
           PF05193: Peptidase M16 inactive domain
          Length = 776

 Score = 62.5 bits (145), Expect = 3e-10
 Identities = 49/207 (23%), Positives = 85/207 (41%), Gaps = 1/207 (0%)
 Frame = +2

Query: 5   LLGHEXXXXXXXXXXXXVWALGIYTGNSESGIDYTSMYSLFSTQVVLTKDGLDHVDEVLE 184
           L+GHE             WA G+Y G +    D++  YS F+  + LT  G +H+ ++L 
Sbjct: 225 LIGHEGEGSLFHALKILGWATGLYAGEA----DWSMEYSFFNVSIDLTDAGHEHMQDILG 280

Query: 185 AIFSYINMLKKIGPSERIYNE-TRHRRDEFSLRRGVPASGLCGDSGREHAVLPARTLHNR 361
            +F YI +L++ G S+ I++E +     EF  +  +       D      + P +     
Sbjct: 281 LLFEYIKVLQQSGVSQWIFDELSAICEAEFHYQAKIDPISYAVDISSNMKIYPTKHWLVG 340

Query: 362 RQTLLQIRSEGITELLDCMTADRVNIMILSNKHSKPIAYDSKEKWFGTETEDKKSQRNGS 541
                +     + ++LD ++ + V I   SNK       D  E W+ T    +K  +   
Sbjct: 341 SSLPSKFNPAIVQKVLDELSPNNVRIFWESNKFEGQT--DKVEPWYNTAYSLEKITKFTI 398

Query: 542 NVG*TPNRGPQFYLPEKNIYITTNFDL 622
                        LP  N++I T+F L
Sbjct: 399 QEWMQSAPDVNLLLPTPNVFIPTDFSL 425



 Score = 37.5 bits (83), Expect = 0.009
 Identities = 12/45 (26%), Positives = 25/45 (55%)
 Frame = +3

Query: 261 ETSFRFEEESQPADYVETLAENMQFFPPEHYITGDRLYYKYDPKV 395
           E  F ++ +  P  Y   ++ NM+ +P +H++ G  L  K++P +
Sbjct: 307 EAEFHYQAKIDPISYAVDISSNMKIYPTKHWLVGSSLPSKFNPAI 351


>At1g06900.1 68414.m00733 peptidase M16 family protein / insulinase
           family protein contains Pfam domain, PF05193: Peptidase
           M16 inactive domain; similar to insulin-degrading enzyme
           (Insulysin, Insulinase, Insulin protease) [Mouse]
           SWISS-PROT:Q9JHR7
          Length = 1023

 Score = 59.7 bits (138), Expect = 2e-09
 Identities = 46/211 (21%), Positives = 87/211 (41%), Gaps = 2/211 (0%)
 Frame = +2

Query: 2   YLLGHEXXXXXXXXXXXXVWALGIYTGNSESGIDYTSMYSLFSTQVVLTKDGLDHVDEVL 181
           +LLGHE             WA  +  G  + GI+ +S+  +F   + LT  GL+ + +++
Sbjct: 356 HLLGHEGRGSLHSFLKAKGWATSLSAGVGDDGINRSSLAYVFGMSIHLTDSGLEKIYDII 415

Query: 182 EAIFSYINMLKKIGPSERIYNETRHRRD-EFSLRRGVPASGLCGDSGREHAVLPARTLHN 358
             I+ Y+ +L+ + P E I+ E +   + +F      PA     +        P   +  
Sbjct: 416 GYIYQYLKLLRDVSPQEWIFKELQDIGNMDFRFAEEQPADDYAAELSENMLAYPVEHVIY 475

Query: 359 RRQTLLQIRSEGITELLDCMTADRVNIMILSNKHSKPIAYDSKEKWFGTETEDKKSQRNG 538
                     + I +L+   T   + I ++S K  K   +  +E WFG+   ++    + 
Sbjct: 476 GDYVYQTWDPKLIEDLMGFFTPQNMRIDVVS-KSIKSEEF-QQEPWFGSSYIEEDVPLSL 533

Query: 539 SNVG*TPNR-GPQFYLPEKNIYITTNFDLIA 628
                 P+      +LP KN +I  +F + A
Sbjct: 534 MESWSNPSEVDNSLHLPSKNQFIPCDFSIRA 564



 Score = 53.6 bits (123), Expect = 1e-07
 Identities = 25/53 (47%), Positives = 30/53 (56%)
 Frame = +3

Query: 237 FTTRQDIEETSFRFEEESQPADYVETLAENMQFFPPEHYITGDRLYYKYDPKV 395
           F   QDI    FRF EE    DY   L+ENM  +P EH I GD +Y  +DPK+
Sbjct: 435 FKELQDIGNMDFRFAEEQPADDYAAELSENMLAYPVEHVIYGDYVYQTWDPKL 487


>At2g41790.1 68415.m05165 peptidase M16 family protein / insulinase
           family protein contains Pfam domain, PF05193: Peptidase
           M16 inactive domain; similar to insulin-degrading enzyme
           (Insulysin, Insulinase, Insulin protease) [Mouse]
           SWISS-PROT:Q9JHR7
          Length = 970

 Score = 56.0 bits (129), Expect = 2e-08
 Identities = 45/208 (21%), Positives = 81/208 (38%), Gaps = 1/208 (0%)
 Frame = +2

Query: 2   YLLGHEXXXXXXXXXXXXVWALGIYTGNSESGIDYTSMYSLFSTQVVLTKDGLDHVDEVL 181
           +L+GHE             WA G+  G  E  +DY    S F   + LT  G +H+ E+L
Sbjct: 297 HLIGHEGEGSLFHALKTLGWATGLSAGEGEWTLDY----SFFKVSIDLTDAGHEHMQEIL 352

Query: 182 EAIFSYINMLKKIGPSERIYNE-TRHRRDEFSLRRGVPASGLCGDSGREHAVLPARTLHN 358
             +F+YI +L++ G  + I++E +     +F  +  +P      D      + P +    
Sbjct: 353 GLLFNYIQLLQQTGVCQWIFDELSAICETKFHYQDKIPPMSYIVDIASNMQIYPTKDWLV 412

Query: 359 RRQTLLQIRSEGITELLDCMTADRVNIMILSNKHSKPIAYDSKEKWFGTETEDKKSQRNG 538
                 +     + +++D ++     I   S K       D  E W+ T    +K   + 
Sbjct: 413 GSSLPTKFNPAIVQKVVDELSPSNFRIFWESQKFEGQT--DKAEPWYNTAYSLEKITSST 470

Query: 539 SNVG*TPNRGPQFYLPEKNIYITTNFDL 622
                        +LP  N++I T+  L
Sbjct: 471 IQEWVQSAPDVHLHLPAPNVFIPTDLSL 498


>At3g57460.1 68416.m06397 expressed protein weak similarity to
           SP|O43847 Nardilysin precursor (EC 3.4.24.61)
           (N-arginine dibasic convertase) {Homo sapiens}
          Length = 356

 Score = 42.7 bits (96), Expect = 2e-04
 Identities = 21/63 (33%), Positives = 36/63 (57%)
 Frame = +2

Query: 59  WALGIYTGNSESGIDYTSMYSLFSTQVVLTKDGLDHVDEVLEAIFSYINMLKKIGPSERI 238
           WA G+Y G      D+T  YS F+  + LT    +H+ ++L  +F  I +L++ G S+ I
Sbjct: 70  WATGLYAGEP----DWTVEYSFFNVSINLTDACHEHMKDILGLLFRQIKLLQQSGVSQWI 125

Query: 239 YNE 247
           ++E
Sbjct: 126 FDE 128


>At5g51300.2 68418.m06360 splicing factor-related contains
           similarity to SF1 protein [Drosophila melanogaster]
           GI:6687400
          Length = 804

 Score = 29.1 bits (62), Expect = 3.0
 Identities = 23/87 (26%), Positives = 40/87 (45%), Gaps = 6/87 (6%)
 Frame = +2

Query: 173 EVLEAIFSYINMLKKI-GPSERIYNETRHR--RDEFSLR---RGVPASGLCGDSGREHAV 334
           E  EA+ +   M++K+  P + + NE + +  R+  +L    R      LCG+ G     
Sbjct: 316 ETQEALEAAAGMVEKLLQPVDEVLNEHKRQQLRELATLNGTIRDEEFCRLCGEPGHRQYA 375

Query: 335 LPARTLHNRRQTLLQIRSEGITELLDC 415
            P+RT   +   L +I  +G    +DC
Sbjct: 376 CPSRTNTFKSDVLCKICGDGGHPTIDC 402


>At5g51300.1 68418.m06359 splicing factor-related contains
           similarity to SF1 protein [Drosophila melanogaster]
           GI:6687400
          Length = 804

 Score = 29.1 bits (62), Expect = 3.0
 Identities = 23/87 (26%), Positives = 40/87 (45%), Gaps = 6/87 (6%)
 Frame = +2

Query: 173 EVLEAIFSYINMLKKI-GPSERIYNETRHR--RDEFSLR---RGVPASGLCGDSGREHAV 334
           E  EA+ +   M++K+  P + + NE + +  R+  +L    R      LCG+ G     
Sbjct: 316 ETQEALEAAAGMVEKLLQPVDEVLNEHKRQQLRELATLNGTIRDEEFCRLCGEPGHRQYA 375

Query: 335 LPARTLHNRRQTLLQIRSEGITELLDC 415
            P+RT   +   L +I  +G    +DC
Sbjct: 376 CPSRTNTFKSDVLCKICGDGGHPTIDC 402


>At3g11590.1 68416.m01416 expressed protein
          Length = 622

 Score = 28.7 bits (61), Expect = 3.9
 Identities = 17/41 (41%), Positives = 24/41 (58%), Gaps = 1/41 (2%)
 Frame = +1

Query: 583 SGEEHLHNDEL*LDSSSFSHTSRRANETRAST*R-TVSVQR 702
           SGE  LH+ EL +D+ S+       N  R ST R ++S+QR
Sbjct: 492 SGESDLHSIELNIDNKSYKWPYGEENRGRKSTPRKSLSLQR 532


>At1g11540.1 68414.m01325 expressed protein  contains Pfam profile:
           PF01925 domain of unknown function DUF81
          Length = 367

 Score = 28.7 bits (61), Expect = 3.9
 Identities = 24/69 (34%), Positives = 33/69 (47%), Gaps = 4/69 (5%)
 Frame = -2

Query: 584 EGKTAGLGSAFNQRSSHFSGISCLLFQCRTISLWNRTLWVWSVCLTIS*C*LCRLS---- 417
           E KTA   SAF      F+ + C LF  R     ++TL  + + LTI  C L  +S    
Sbjct: 8   EMKTASSFSAFMVTGVSFANVGCNLF-LRNPKSRDKTLIDFDLALTIQPCLLLGVSIGVI 66

Query: 416 CNPIVP*YL 390
           CN + P +L
Sbjct: 67  CNRMFPNWL 75


>At2g17000.1 68415.m01960 mechanosensitive ion channel
           domain-containing protein / MS ion channel
           domain-containing protein contains Pfam profile PF00924:
           Mechanosensitive ion channel
          Length = 849

 Score = 28.3 bits (60), Expect = 5.2
 Identities = 24/90 (26%), Positives = 38/90 (42%), Gaps = 1/90 (1%)
 Frame = -2

Query: 524 ISCLLFQCRTISLWNRTLWVWSVCLTIS*C*LCRLSCN-PIVP*YLRIVFVVESVAGYVV 348
           +S  L   R  +LW+  LW W V L +  C      C   I+  ++   F++     Y V
Sbjct: 249 LSLGLHTWRNATLWSLHLWKWEVVLLVLICGRLVSGCGIRIIVFFIERNFLLRKRVLYFV 308

Query: 347 FGREELHVLGQSLHIVRWLGLLFEAKTRLF 258
           +G      +  ++    WLGL+  A   LF
Sbjct: 309 YG------VKTAVQNCLWLGLVLLAWHFLF 332


>At5g04230.1 68418.m00412 phenylalanine ammonia-lyase 3 (PAL3)
           nearly identical to SP|P45725
          Length = 698

 Score = 27.5 bits (58), Expect = 9.1
 Identities = 14/38 (36%), Positives = 21/38 (55%), Gaps = 3/38 (7%)
 Frame = -3

Query: 247 LVVNPFRRSDFL*HVYVREYSFEYLID---VVEPVLSK 143
           L + PFR+ D +  V  REY F Y+ D   +  P++ K
Sbjct: 540 LAIEPFRKHDDILGVVNREYVFSYVDDPSSLTNPLMQK 577


>At1g80510.1 68414.m09435 amino acid transporter family protein
           similar to amino acid transporter system N2 [Rattus
           norvegicus] GI:14578932; contains Pfam profile PF01490:
           Transmembrane amino acid transporter protein
          Length = 489

 Score = 27.5 bits (58), Expect = 9.1
 Identities = 19/64 (29%), Positives = 35/64 (54%), Gaps = 5/64 (7%)
 Frame = -2

Query: 386 IVFVVESVAGYVVFGRE-ELHVLGQSLHIVRWLGLLFEAK----TRLFYVLSRCKSFPKV 222
           +V+   +V+GY++FG++ E  +L    +  + LG+ F +      R+ Y+L     FP +
Sbjct: 317 VVYASTAVSGYLLFGKDTESDIL---TNFDQDLGIRFSSAVNYIVRIGYILHLVLVFPVI 373

Query: 221 RFSL 210
            FSL
Sbjct: 374 HFSL 377


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,310,439
Number of Sequences: 28952
Number of extensions: 326050
Number of successful extensions: 924
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 860
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 923
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1506636208
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)