Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30464
(826 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 160 2e-40
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 157 2e-39
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 97 2e-21
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 70 5e-13
SPBC354.07c |||oxysterol binding protein |Schizosaccharomyces po... 29 0.80
SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr... 29 1.1
SPBC2F12.13 |klp5|sot1|kinesin-like protein Klp5|Schizosaccharom... 28 1.4
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 7.5
SPAC823.06 |taf3||transcription factor TFIID complex subunit Taf... 25 9.9
SPBC119.07 |ppk19||serine/threonine protein kinase Ppk19|Schizos... 25 9.9
SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pom... 25 9.9
SPAC22F3.06c |lon1||Lon protease homolog Lon1|Schizosaccharomyce... 25 9.9
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 160 bits (389), Expect = 2e-40
Identities = 71/95 (74%), Positives = 85/95 (89%)
Frame = +3
Query: 510 GTGSGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFM 689
GTGSG +LL+ERL+++YGKKS L+F++YPAPQVST+VVEPYNS+LTTH TL++SDC FM
Sbjct: 148 GTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFM 207
Query: 690 VDNEAIYDICRRNLDIERPTYNQPDRLIGQIVSSI 794
VDNEA YDICRRNLDIERPTY +RLI Q+VSSI
Sbjct: 208 VDNEACYDICRRNLDIERPTYENLNRLIAQVVSSI 242
Score = 153 bits (372), Expect = 2e-38
Identities = 63/94 (67%), Positives = 80/94 (85%)
Frame = +1
Query: 226 FQHFLQRDRSGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHY 405
F F GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHY
Sbjct: 53 FGTFFSETGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHY 112
Query: 406 TIGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFG 507
T+GKE++D VL+RIR++AD C+GLQGFL+FHSFG
Sbjct: 113 TVGKEMIDSVLERIRRMADNCSGLQGFLVFHSFG 146
Score = 87.8 bits (208), Expect = 2e-18
Identities = 45/68 (66%), Positives = 47/68 (69%), Gaps = 5/68 (7%)
Frame = +2
Query: 83 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 247
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 248 TGVASTYP 271
TG P
Sbjct: 60 TGQGKFVP 67
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 157 bits (380), Expect = 2e-39
Identities = 66/94 (70%), Positives = 80/94 (85%)
Frame = +1
Query: 226 FQHFLQRDRSGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHY 405
F F GK+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHY
Sbjct: 49 FSTFFSETGQGKYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHY 108
Query: 406 TIGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFG 507
T+GKE+VD V D+IR++AD C+GLQGFL+FHSFG
Sbjct: 109 TVGKELVDEVTDKIRRIADNCSGLQGFLVFHSFG 142
Score = 157 bits (380), Expect = 2e-39
Identities = 71/105 (67%), Positives = 89/105 (84%)
Frame = +3
Query: 510 GTGSGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFM 689
GTGSGF +LL+ERL+++Y KKSKL+F++YPAPQVST+VVEPYNS+LTTH TL+ +DC FM
Sbjct: 144 GTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFM 203
Query: 690 VDNEAIYDICRRNLDIERPTYNQPDRLIGQIVSSINCFFEEFDGA 824
VDNE+ YDICRRNLDIERP+Y +RLI Q+VSSI F+G+
Sbjct: 204 VDNESCYDICRRNLDIERPSYENLNRLIAQVVSSITASL-RFEGS 247
Score = 93.9 bits (223), Expect = 2e-20
Identities = 41/63 (65%), Positives = 45/63 (71%)
Frame = +2
Query: 83 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGVAS 262
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 263 TYP 271
P
Sbjct: 61 YVP 63
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 97.5 bits (232), Expect = 2e-21
Identities = 43/84 (51%), Positives = 58/84 (69%)
Frame = +1
Query: 256 GKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLV 435
GK+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D V
Sbjct: 57 GKYVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAV 116
Query: 436 LDRIRKLADQCTGLQGFLIFHSFG 507
LD +R+ A+ C LQGF + HS G
Sbjct: 117 LDVVRREAEACDALQGFQLTHSLG 140
Score = 81.8 bits (193), Expect = 1e-16
Identities = 36/99 (36%), Positives = 60/99 (60%)
Frame = +3
Query: 510 GTGSGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFM 689
GTGSG +LL+ ++ +Y + F++ PAP+ S VVEPYN+ L+ H +E+SD F
Sbjct: 142 GTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFC 201
Query: 690 VDNEAIYDICRRNLDIERPTYNQPDRLIGQIVSSINCFF 806
+DNEA+ I L I+ P+Y+ + L+ +++ + F
Sbjct: 202 IDNEALSSIFANTLKIKSPSYDDLNHLVSAVMAGVTTSF 240
Score = 35.1 bits (77), Expect = 0.012
Identities = 19/63 (30%), Positives = 26/63 (41%)
Frame = +2
Query: 83 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGVAS 262
MRE + + GQ G Q+G A W EHG+ G T + N +F+E
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 263 TYP 271
P
Sbjct: 59 YVP 61
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 69.7 bits (163), Expect = 5e-13
Identities = 35/95 (36%), Positives = 57/95 (60%), Gaps = 1/95 (1%)
Frame = +3
Query: 510 GTGSGFTSLLMERLSVDYGKKSKLEFAIYPAPQ-VSTAVVEPYNSILTTHTTLEHSDCAF 686
GTGSG S L+ERL+ Y KK ++++P Q VS VV+PYNS+L ++D
Sbjct: 144 GTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVV 203
Query: 687 MVDNEAIYDICRRNLDIERPTYNQPDRLIGQIVSS 791
++DN A+ I L + PT++Q ++L+ ++S+
Sbjct: 204 VLDNAALAHIAADRLHTQNPTFHQQNQLVSTVMSA 238
Score = 67.7 bits (158), Expect = 2e-12
Identities = 38/102 (37%), Positives = 60/102 (58%), Gaps = 4/102 (3%)
Frame = +1
Query: 235 FLQRDRSGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYT 408
F Q D + +++PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+
Sbjct: 52 FYQSDDT-RYIPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YS 109
Query: 409 IGKEIVDLVLDRIRKLADQCTGLQGFLIFHSF--GEVPALGS 528
+ I + ++D I + AD L+GF + HS G LGS
Sbjct: 110 HAERIFEDIMDMIDREADGSDSLEGFSLLHSIAGGTGSGLGS 151
Score = 48.8 bits (111), Expect = 9e-07
Identities = 24/55 (43%), Positives = 33/55 (60%)
Frame = +2
Query: 86 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSET 250
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQS 55
>SPBC354.07c |||oxysterol binding protein |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 399
Score = 29.1 bits (62), Expect = 0.80
Identities = 15/58 (25%), Positives = 26/58 (44%), Gaps = 2/58 (3%)
Frame = -2
Query: 351 FWMKQLSVCASADLINNSRF--KIDEDSTGYVLATPVSLKKVLKESSPPPMVLSVGIW 184
F + + S +++N+ + KID GY T S K + E + P + G+W
Sbjct: 205 FMAPYIELYGSTYIVSNTNYITKIDYSGRGYFRGTKNSFKATIFEKNEDPDYIVEGVW 262
>SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1024
Score = 28.7 bits (61), Expect = 1.1
Identities = 13/38 (34%), Positives = 18/38 (47%)
Frame = -2
Query: 318 ADLINNSRFKIDEDSTGYVLATPVSLKKVLKESSPPPM 205
AD+IN SR+ ID S +T + L P P+
Sbjct: 455 ADIINGSRYNIDRVSPNTAFSTNIPLSLPFANKEPQPI 492
>SPBC2F12.13 |klp5|sot1|kinesin-like protein
Klp5|Schizosaccharomyces pombe|chr 2|||Manual
Length = 883
Score = 28.3 bits (60), Expect = 1.4
Identities = 15/36 (41%), Positives = 21/36 (58%)
Frame = +1
Query: 706 SMTSAAVISTLSAQPTTNLIVSSDRLSPRSIASLKN 813
S+T + S + NLI SSDRLS + +SL+N
Sbjct: 6 SITVTVRVRPFSTAESANLIASSDRLSFGTSSSLRN 41
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 7.5
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +1
Query: 181 WPDAHRQDHRGWRRFFQHFLQR 246
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
>SPAC823.06 |taf3||transcription factor TFIID complex subunit
Taf3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 155
Score = 25.4 bits (53), Expect = 9.9
Identities = 10/37 (27%), Positives = 20/37 (54%)
Frame = -3
Query: 782 NLSDETIRLVVGWALNVEITAADVIDGFIVDHESTVR 672
N S+E ++ +V W ++ + GF+ D E+ V+
Sbjct: 81 NGSEEDVKTLVEWFNGPQVAELRRVSGFVQDSETQVK 117
>SPBC119.07 |ppk19||serine/threonine protein kinase
Ppk19|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1706
Score = 25.4 bits (53), Expect = 9.9
Identities = 13/40 (32%), Positives = 19/40 (47%)
Frame = +1
Query: 184 PDAHRQDHRGWRRFFQHFLQRDRSGKHVPRAVFVDLEPTV 303
P+ H + G F + F +DR G V VFV+ P +
Sbjct: 22 PEYHNERSLGDSHFLRTFRMQDRKGYDVLIKVFVNKLPEI 61
>SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 534
Score = 25.4 bits (53), Expect = 9.9
Identities = 15/41 (36%), Positives = 21/41 (51%)
Frame = +3
Query: 72 LKSKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCP 194
L+S +L+ P SR++ P S + STASSL P
Sbjct: 170 LRSSMPLVMANSLSPPSSRALKPIHSLSNPSTASSLEPSSP 210
>SPAC22F3.06c |lon1||Lon protease homolog Lon1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1067
Score = 25.4 bits (53), Expect = 9.9
Identities = 12/37 (32%), Positives = 22/37 (59%)
Frame = -2
Query: 285 DEDSTGYVLATPVSLKKVLKESSPPPMVLSVGIWPSG 175
++D T Y L P+ + L +++PP +V+ +G P G
Sbjct: 834 EKDLTKY-LGPPIYTSQRLYDTTPPGVVMGLGWTPMG 869
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,394,074
Number of Sequences: 5004
Number of extensions: 72245
Number of successful extensions: 234
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 216
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 231
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 404442380
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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