BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30460 (788 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 138 6e-34 SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 63 5e-11 SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 63 5e-11 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 43 6e-05 SPBC354.13 |rga6||GTPase activating protein Rga6|Schizosaccharom... 30 0.43 SPBC557.04 |ppk29||Ark1/Prk1 family protein kinase Ppk29|Schizos... 28 1.3 SPAC22E12.04 |ccs1|pccs, pccs|metallochaperone Ccs1 |Schizosacch... 27 4.0 SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein Ubr1|Sc... 26 5.4 SPAC4F10.02 |||aminopeptidase |Schizosaccharomyces pombe|chr 1||... 26 5.4 SPBC29A3.15c |rsm23||mitochondrial ribosomal protein subunit S23... 26 5.4 SPBC31E1.04 |pep12||SNARE Pep12|Schizosaccharomyces pombe|chr 2|... 26 5.4 SPBC211.03c |||guanyl-nucleotide exchange factor|Schizosaccharom... 25 9.4 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 138 bits (335), Expect = 6e-34 Identities = 60/71 (84%), Positives = 69/71 (97%) Frame = +1 Query: 1 IPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNM 180 +PP+ LKM+ATFIGNST+IQE+F+R+ +QF+AMFRRKAFLHWYTGEGMDEMEFTEAESNM Sbjct: 356 VPPKDLKMSATFIGNSTSIQEIFRRLGDQFSAMFRRKAFLHWYTGEGMDEMEFTEAESNM 415 Query: 181 NDLVSEYQQYQ 213 NDLVSEYQQYQ Sbjct: 416 NDLVSEYQQYQ 426 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 62.9 bits (146), Expect = 5e-11 Identities = 24/61 (39%), Positives = 41/61 (67%) Frame = +1 Query: 25 AATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQ 204 A + N+T+I E + R+ +F M+ ++AF+HWY GEGM+E EF+EA ++ L +Y+ Sbjct: 378 AVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALERDYE 437 Query: 205 Q 207 + Sbjct: 438 E 438 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 62.9 bits (146), Expect = 5e-11 Identities = 24/61 (39%), Positives = 41/61 (67%) Frame = +1 Query: 25 AATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQ 204 A + N+T+I E + R+ +F M+ ++AF+HWY GEGM+E EF+EA ++ L +Y+ Sbjct: 374 AVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALERDYE 433 Query: 205 Q 207 + Sbjct: 434 E 434 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 42.7 bits (96), Expect = 6e-05 Identities = 21/65 (32%), Positives = 38/65 (58%), Gaps = 3/65 (4%) Frame = +1 Query: 19 KMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMN---DL 189 +++ + N T+I LFKR +Q+ + +R AFL Y E + E + E +S+ + DL Sbjct: 372 RVSGLMLANHTSIASLFKRTLDQYDRLRKRNAFLEQYKKEAIFEDDLNEFDSSRDVVADL 431 Query: 190 VSEYQ 204 ++EY+ Sbjct: 432 INEYE 436 >SPBC354.13 |rga6||GTPase activating protein Rga6|Schizosaccharomyces pombe|chr 2|||Manual Length = 733 Score = 29.9 bits (64), Expect = 0.43 Identities = 18/68 (26%), Positives = 34/68 (50%) Frame = -2 Query: 349 GSLERREVERGLFNIETRASSERT*LSSISCSAPRQTLRPRRRWPPGTVGTRRQGRSCCS 170 G L R + + E + S + LS+ + + TL P + +PP + G++RQ + Sbjct: 182 GPLGRSSLNLSSLSHELQTSQDSPSLSATNQLSSSDTLEPLQ-YPPSSFGSQRQFNASQD 240 Query: 169 QPQ*TPSR 146 P+ +PS+ Sbjct: 241 SPKRSPSK 248 >SPBC557.04 |ppk29||Ark1/Prk1 family protein kinase Ppk29|Schizosaccharomyces pombe|chr 2|||Manual Length = 872 Score = 28.3 bits (60), Expect = 1.3 Identities = 14/36 (38%), Positives = 21/36 (58%) Frame = +1 Query: 43 NSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDE 150 NS+ IQ L K I+ T +R ++ Y+G G+DE Sbjct: 193 NSSEIQALEKSINTFTTYQYRAPEMINLYSGLGIDE 228 >SPAC22E12.04 |ccs1|pccs, pccs|metallochaperone Ccs1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 297 Score = 26.6 bits (56), Expect = 4.0 Identities = 11/28 (39%), Positives = 16/28 (57%) Frame = -2 Query: 190 QGRSCCSQPQ*TPSRPCPRRCTNARKPC 107 +G SCCS+ +PS P C+ +K C Sbjct: 221 EGSSCCSKKDSSPSEK-PSCCSQEKKSC 247 >SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein Ubr1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1958 Score = 26.2 bits (55), Expect = 5.4 Identities = 18/59 (30%), Positives = 29/59 (49%) Frame = +1 Query: 37 IGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ 213 + N + L+K + E+F+ +F RK L WY G+ E + N+N SE + Q Sbjct: 1703 LNNPHLLFTLYKLL-ERFSLIFLRKCALLWYCRYGVS----FETQPNLNFQNSELSRLQ 1756 >SPAC4F10.02 |||aminopeptidase |Schizosaccharomyces pombe|chr 1|||Manual Length = 467 Score = 26.2 bits (55), Expect = 5.4 Identities = 9/23 (39%), Positives = 14/23 (60%) Frame = -3 Query: 384 SYYINTKSKSIVDLSKGERWKGG 316 SY++ SI+ S G++WK G Sbjct: 56 SYFVTRNKSSIIAFSIGKKWKPG 78 >SPBC29A3.15c |rsm23||mitochondrial ribosomal protein subunit S23|Schizosaccharomyces pombe|chr 2|||Manual Length = 476 Score = 26.2 bits (55), Expect = 5.4 Identities = 13/46 (28%), Positives = 25/46 (54%) Frame = -3 Query: 540 ASLRVAVYCNNIPELPFVVKEKHRECKHNLLPRHNVYFLGYFIMHV 403 ++++V + +NI L V K K + K+N LP + YF+ ++ Sbjct: 326 SNMKVLLVIDNISILSVVTKYKDK--KNNFLPPKDFYFINLLFKYI 369 >SPBC31E1.04 |pep12||SNARE Pep12|Schizosaccharomyces pombe|chr 2|||Manual Length = 317 Score = 26.2 bits (55), Expect = 5.4 Identities = 13/27 (48%), Positives = 17/27 (62%) Frame = +3 Query: 357 FCFSYLYSNFNSFRLQHA**NNLGSTR 437 FCF ++ F+SFR Q+A NL S R Sbjct: 243 FCFLKSFAMFSSFRSQNANLYNLNSIR 269 >SPBC211.03c |||guanyl-nucleotide exchange factor|Schizosaccharomyces pombe|chr 2|||Manual Length = 1462 Score = 25.4 bits (53), Expect = 9.4 Identities = 16/48 (33%), Positives = 23/48 (47%), Gaps = 3/48 (6%) Frame = -3 Query: 141 ALAGVPMQESLAPEHSGEL---FRDALEQLLDGGGVSNEGGGHLETTR 7 A G+P +S+ HS EL F L+ ++ SNE H + TR Sbjct: 1212 ASKGLPFDKSILDNHSAELNDAFNLFLKAAVEYKVDSNESSEHFQDTR 1259 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,966,772 Number of Sequences: 5004 Number of extensions: 54423 Number of successful extensions: 134 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 129 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 134 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 383374054 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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