Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30457
(749 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g67270.1 68418.m08480 microtubule-associated EB1 family prote... 63 2e-10
At5g62500.1 68418.m07844 microtubule-associated EB1 family prote... 57 1e-08
At3g47690.1 68416.m05194 microtubule-associated EB1 family prote... 53 2e-07
At2g03200.1 68415.m00273 aspartyl protease family protein contai... 30 1.4
At5g46760.1 68418.m05760 basic helix-loop-helix (bHLH) family pr... 28 5.8
At5g05510.1 68418.m00598 protein kinase-related low similarity t... 28 5.8
At3g44690.1 68416.m04806 expressed protein 28 5.8
At2g11010.1 68415.m01178 hypothetical protein 28 5.8
At1g63840.1 68414.m07226 zinc finger (C3HC4-type RING finger) fa... 28 5.8
At4g10370.1 68417.m01702 DC1 domain-containing protein contains ... 28 7.6
At3g45540.1 68416.m04918 zinc finger (C3HC4-type RING finger) fa... 28 7.6
>At5g67270.1 68418.m08480 microtubule-associated EB1 family protein
similar to SP|Q9UPY8 Microtubule-associated protein
RP/EB family member 3 (Protein EB3) {Homo sapiens};
contains Pfam profiles PF00307: Calponin homology (CH)
domain, PF03271: EB1 protein
Length = 329
Score = 63.3 bits (147), Expect = 2e-10
Identities = 52/162 (32%), Positives = 69/162 (42%), Gaps = 10/162 (6%)
Frame = +1
Query: 214 VPIDKLVKGRFQDNLSFCNGSRNSLTPTMEVRHMTQWRTRRSAH--GARGLGRP-----E 372
+ + KLVKGR DNL F + +H RR A G R
Sbjct: 90 IEVSKLVKGRPLDNLEFMQWMKKYCDSVNGGQHNYHALERREASKGGKEATKRAAATQQS 149
Query: 373 GRCR*ETRRPCRQSRS*TPNHWES*YNS---QISTS*FVTDKSKCQG*SKVVDELNHQIN 543
G+ + P S + T H N+ ST SK V + +I
Sbjct: 150 GKSSSSSAPPRPSSSNGTRKHEPQSNNTGTHHSSTGNHHHSSKPSAKQSKPVPAYDEKIT 209
Query: 544 ELKATVDGLEKERDFYFGKLRDIEVICQEMEEQHNAPIVQKI 669
ELK +D LEKERDFYF KLRD+E++CQ + +H P+V I
Sbjct: 210 ELKLYIDSLEKERDFYFSKLRDVEILCQNPDTEH-LPLVGSI 250
Score = 31.9 bits (69), Expect = 0.47
Identities = 14/29 (48%), Positives = 19/29 (65%), Gaps = 1/29 (3%)
Frame = +3
Query: 15 DNFEFLQWFKKFFDANYDGR-DYDAFEAR 98
DN EF+QW KK+ D+ G+ +Y A E R
Sbjct: 102 DNLEFMQWMKKYCDSVNGGQHNYHALERR 130
>At5g62500.1 68418.m07844 microtubule-associated EB1 family protein
similar to EBF3-S (Microtubule-associated protein) [Homo
sapiens] GI:12751131; contains Pfam profiles PF00307:
Calponin homology (CH) domain, PF03271: EB1 protein
Length = 293
Score = 57.2 bits (132), Expect = 1e-08
Identities = 28/51 (54%), Positives = 36/51 (70%)
Frame = +1
Query: 508 SKVVDELNHQINELKATVDGLEKERDFYFGKLRDIEVICQEMEEQHNAPIV 660
S V L+ ++ +LK +VD LEKERDFYF KLRDIE++CQ E + PIV
Sbjct: 185 SAEVQALSKEVEDLKVSVDLLEKERDFYFSKLRDIEILCQ-TPELDDLPIV 234
Score = 29.1 bits (62), Expect = 3.3
Identities = 14/31 (45%), Positives = 18/31 (58%), Gaps = 3/31 (9%)
Frame = +3
Query: 15 DNFEFLQWFKKFFDANYDG---RDYDAFEAR 98
DN EFLQW K+F D+ G +Y+ E R
Sbjct: 102 DNLEFLQWLKRFCDSINGGIMNENYNPVERR 132
>At3g47690.1 68416.m05194 microtubule-associated EB1 family protein
similar to SP|Q9UPY8 Microtubule-associated protein
RP/EB family member 3 (Protein EB3) {Homo sapiens};
contains Pfam profile PF03271: EB1 protein
Length = 276
Score = 52.8 bits (121), Expect = 2e-07
Identities = 25/48 (52%), Positives = 34/48 (70%)
Frame = +1
Query: 517 VDELNHQINELKATVDGLEKERDFYFGKLRDIEVICQEMEEQHNAPIV 660
V L+ ++ +LK + D LEKERDFYF KLRD+E++CQ E + PIV
Sbjct: 181 VQALSKELVDLKISTDLLEKERDFYFSKLRDVEILCQ-TPELDDLPIV 227
Score = 29.1 bits (62), Expect = 3.3
Identities = 14/31 (45%), Positives = 18/31 (58%), Gaps = 3/31 (9%)
Frame = +3
Query: 15 DNFEFLQWFKKFFDANYDG---RDYDAFEAR 98
DN EFLQW K+F D+ G +Y+ E R
Sbjct: 102 DNLEFLQWLKRFCDSINGGIMNENYNPVERR 132
>At2g03200.1 68415.m00273 aspartyl protease family protein contains
Pfam domain, PF00026: eukaryotic aspartyl protease
Length = 461
Score = 30.3 bits (65), Expect = 1.4
Identities = 14/30 (46%), Positives = 18/30 (60%), Gaps = 1/30 (3%)
Frame = -2
Query: 436 NGLGSSCDFGDRGDGFLSGSGPRG-GRAPV 350
+G+G C + GDGF GSG G GR P+
Sbjct: 212 SGIGFGCGVENEGDGFSQGSGLVGLGRGPL 241
>At5g46760.1 68418.m05760 basic helix-loop-helix (bHLH) family
protein
Length = 592
Score = 28.3 bits (60), Expect = 5.8
Identities = 16/42 (38%), Positives = 26/42 (61%), Gaps = 2/42 (4%)
Frame = +1
Query: 169 QREHTDQRPYSIRAIVP-IDKLVKGR-FQDNLSFCNGSRNSL 288
+RE +QR YS+RA+VP + K+ K D +S+ N ++ L
Sbjct: 423 RREKLNQRFYSLRAVVPNVSKMDKASLLGDAISYINELKSKL 464
>At5g05510.1 68418.m00598 protein kinase-related low similarity to
SP|O60566 Mitotic checkpoint serine/threonine-protein
kinase BUB1 beta (EC 2.7.1.-) {Homo sapiens}
Length = 471
Score = 28.3 bits (60), Expect = 5.8
Identities = 12/27 (44%), Positives = 18/27 (66%)
Frame = +3
Query: 492 KVPRVIESCG*IKPSDKRIESHSRWIR 572
K+PR ++ C SDKR ++ SR+IR
Sbjct: 50 KLPRFLQKCAESFESDKRYKNDSRYIR 76
>At3g44690.1 68416.m04806 expressed protein
Length = 1176
Score = 28.3 bits (60), Expect = 5.8
Identities = 9/28 (32%), Positives = 19/28 (67%)
Frame = +1
Query: 508 SKVVDELNHQINELKATVDGLEKERDFY 591
S +D ++H+++E K +DG+ ++ D Y
Sbjct: 41 SDKIDMVSHRVDEQKHDIDGIRQDSDLY 68
>At2g11010.1 68415.m01178 hypothetical protein
Length = 693
Score = 28.3 bits (60), Expect = 5.8
Identities = 13/23 (56%), Positives = 15/23 (65%)
Frame = -1
Query: 239 PFTSLSMGTIARMLYGRWSVCSR 171
P SLS GT+AR L RW V S+
Sbjct: 275 PPPSLSQGTLARDLASRWKVISK 297
>At1g63840.1 68414.m07226 zinc finger (C3HC4-type RING finger)
family protein similar to RING-H2 finger protein RHA1a
(GI:3790554) [Arabidopsis thaliana]' similar to BRH1
RING finger protein [Arabidopsis thaliana] GI:4689366;
contains Pfam profile PF00097: Zinc finger, C3HC4 type
(RING finger)
Length = 166
Score = 28.3 bits (60), Expect = 5.8
Identities = 15/47 (31%), Positives = 25/47 (53%)
Frame = -1
Query: 332 LVRHCVICRTSIVGVKEFLEPLQKLKLSWKRPFTSLSMGTIARMLYG 192
L+R + I+G+ +FLEP + + SW P +L+ A +L G
Sbjct: 25 LIRKLISTMFKIIGLPDFLEP-EPVSTSWPDPPPTLTKPDSAAILAG 70
>At4g10370.1 68417.m01702 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 687
Score = 27.9 bits (59), Expect = 7.6
Identities = 11/24 (45%), Positives = 14/24 (58%)
Frame = -2
Query: 676 IKKSFAL*EHCVVLPSLDRSPLYP 605
I+ F L EHC P + R PL+P
Sbjct: 399 IESDFILHEHCANAPRMKRHPLHP 422
>At3g45540.1 68416.m04918 zinc finger (C3HC4-type RING finger)
family protein contains a Prosite:PS00518 Zinc finger,
C3HC4 type (RING finger), signature
Length = 348
Score = 27.9 bits (59), Expect = 7.6
Identities = 12/28 (42%), Positives = 13/28 (46%)
Frame = -2
Query: 184 RCALVGGGCTMPHNGTPRSQMPEPSGIP 101
RC + GGC H PR P PS P
Sbjct: 291 RCGVQAGGCKHGHGLPPRPPPPPPSPPP 318
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,456,379
Number of Sequences: 28952
Number of extensions: 404799
Number of successful extensions: 1187
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 1092
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1187
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1663169840
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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