BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30455 (760 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 155 5e-39 SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 154 1e-38 SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 79 7e-16 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 61 2e-10 SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom... 33 0.044 SPBC428.07 |meu6||meiotic chromosome segregation protein Meu6|Sc... 32 0.077 SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 31 0.18 SPCC18.03 |||shuttle craft like transcriptional regulator|Schizo... 30 0.31 SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 30 0.41 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 30 0.41 SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1||... 28 1.3 SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|ch... 26 5.1 SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch... 26 6.7 SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces po... 25 8.9 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 25 8.9 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 155 bits (377), Expect = 5e-39 Identities = 71/84 (84%), Positives = 76/84 (90%) Frame = +3 Query: 3 SSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQXVSSITASLRFDGALNVDLTEFQ 182 +SDC FMVDNEA YDICRRNLDIERPTY NLNRLI Q VSSITASLRF G+LNVDL EFQ Sbjct: 201 NSDCTFMVDNEACYDICRRNLDIERPTYENLNRLIAQVVSSITASLRFAGSLNVDLNEFQ 260 Query: 183 TNLVPYPRIHFPLVTYAPVISAEK 254 TNLVPYPRIHFPLVTY+P++SA K Sbjct: 261 TNLVPYPRIHFPLVTYSPIVSAAK 284 Score = 150 bits (363), Expect = 2e-37 Identities = 63/93 (67%), Positives = 77/93 (82%) Frame = +2 Query: 251 EAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKR 430 +A+HE SV EITN CFEP NQMVKCDPR G+YMA C+LYRGDV+P+DV AA+ +IK++R Sbjct: 284 KAFHESNSVQEITNQCFEPYNQMVKCDPRTGRYMATCLLYRGDVIPRDVQAAVTSIKSRR 343 Query: 431 TIQFVDWCPTGFKVGINYQPPTVVPGATWPRFN 529 TIQFVDWCPTGFK+GI Y+PP VPG+ + N Sbjct: 344 TIQFVDWCPTGFKIGICYEPPQHVPGSGIAKVN 376 Score = 126 bits (303), Expect = 5e-30 Identities = 57/65 (87%), Positives = 61/65 (93%) Frame = +1 Query: 508 SDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDL 687 S +AKV RAVCMLSNTT+IAEAW+RLDHKFDLMY+KRAFVHWYVGEGMEEGEFSEAREDL Sbjct: 370 SGIAKVNRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDL 429 Query: 688 AASRR 702 AA R Sbjct: 430 AALER 434 Score = 25.8 bits (54), Expect = 6.7 Identities = 10/12 (83%), Positives = 11/12 (91%) Frame = +3 Query: 693 LEKDYEEVGMDS 728 LE+DYEEVG DS Sbjct: 432 LERDYEEVGQDS 443 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 154 bits (374), Expect = 1e-38 Identities = 75/120 (62%), Positives = 85/120 (70%) Frame = +3 Query: 6 SDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQXVSSITASLRFDGALNVDLTEFQT 185 +DC FMVDNE+ YDICRRNLDIERP+Y NLNRLI Q VSSITASLRF+G+LNVDL EFQT Sbjct: 198 ADCTFMVDNESCYDICRRNLDIERPSYENLNRLIAQVVSSITASLRFEGSLNVDLAEFQT 257 Query: 186 NLVPYPRIHFPLVTYAPVISAEKPTMNSFPSPRSQTHASSPPTRW*NATPVMASTWLAVC 365 NLVPYPRIHFPLVTYAP++SA K S P + P A ++A C Sbjct: 258 NLVPYPRIHFPLVTYAPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDP-RAGRYMATC 316 Score = 144 bits (348), Expect = 2e-35 Identities = 61/87 (70%), Positives = 72/87 (82%) Frame = +2 Query: 251 EAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKR 430 +A+HE SV EITN CFEP NQMVKCDPR G+YMA C+LYRGDV+P+DV AA+ TIK KR Sbjct: 280 KAFHESNSVQEITNQCFEPYNQMVKCDPRAGRYMATCLLYRGDVIPRDVQAAVTTIKAKR 339 Query: 431 TIQFVDWCPTGFKVGINYQPPTVVPGA 511 TIQFVDWCPTGFK+GI +PP + G+ Sbjct: 340 TIQFVDWCPTGFKIGICDRPPQHIEGS 366 Score = 127 bits (306), Expect = 2e-30 Identities = 57/65 (87%), Positives = 62/65 (95%) Frame = +1 Query: 508 SDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDL 687 S++AKV RAVCMLSNTT+IAEAW+RLDHKFDLMY+KRAFVHWYVGEGMEEGEFSEAREDL Sbjct: 366 SEIAKVDRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDL 425 Query: 688 AASRR 702 AA R Sbjct: 426 AALER 430 Score = 25.8 bits (54), Expect = 6.7 Identities = 10/12 (83%), Positives = 11/12 (91%) Frame = +3 Query: 693 LEKDYEEVGMDS 728 LE+DYEEVG DS Sbjct: 428 LERDYEEVGQDS 439 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 79.0 bits (186), Expect = 7e-16 Identities = 35/93 (37%), Positives = 55/93 (59%) Frame = +3 Query: 3 SSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQXVSSITASLRFDGALNVDLTEFQ 182 +SD F +DNEA+ I L I+ P+Y +LN L+ ++ +T S RF G LN DL + Sbjct: 195 NSDETFCIDNEALSSIFANTLKIKSPSYDDLNHLVSAVMAGVTTSFRFPGELNSDLRKLA 254 Query: 183 TNLVPYPRIHFPLVTYAPVISAEKPTMNSFPSP 281 N+VP+PR+HF +V +AP+ + + + P Sbjct: 255 VNMVPFPRLHFFMVGFAPLAAIGSSSFQAVSVP 287 Score = 66.9 bits (156), Expect = 3e-12 Identities = 29/77 (37%), Positives = 44/77 (57%) Frame = +2 Query: 263 EQLSVAEITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQF 442 + +SV E+T F+ N MV DPRHG+Y+ L+RG V K+V+ I +++TK + F Sbjct: 282 QAVSVPELTQQMFDANNMMVAADPRHGRYLTVAALFRGKVSMKEVDEQIRSVQTKNSAYF 341 Query: 443 VDWCPTGFKVGINYQPP 493 V+W P + PP Sbjct: 342 VEWIPDNVLKAVCSVPP 358 Score = 58.0 bits (134), Expect = 1e-09 Identities = 22/55 (40%), Positives = 38/55 (69%) Frame = +1 Query: 523 VQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDL 687 ++ + + N+T+I E + RL +F M+ ++AF+HWY GEGM+E EF+EA ++ Sbjct: 361 LKMSATFIGNSTSIQEIFRRLGDQFSAMFRRKAFLHWYTGEGMDEMEFTEAESNM 415 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 60.9 bits (141), Expect = 2e-10 Identities = 27/78 (34%), Positives = 44/78 (56%) Frame = +3 Query: 3 SSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQXVSSITASLRFDGALNVDLTEFQ 182 ++D ++DN A+ I L + PT+ N+L+ +S+ T +LR+ G +N DL Sbjct: 198 NADSVVVLDNAALAHIAADRLHTQNPTFHQQNQLVSTVMSASTTTLRYPGYMNNDLVSII 257 Query: 183 TNLVPYPRIHFPLVTYAP 236 +L+P PR HF L +Y P Sbjct: 258 ASLIPSPRCHFLLTSYTP 275 Score = 41.5 bits (93), Expect = 1e-04 Identities = 18/77 (23%), Positives = 40/77 (51%), Gaps = 1/77 (1%) Frame = +2 Query: 272 SVAEITNACFEPANQMVKCDP-RHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVD 448 +V ++ P NQMV +P + +++ + +G+ P DV+ ++ I+ +R F+ Sbjct: 291 TVLDVMRRLLLPKNQMVSVNPSKKSCFISILDIIQGEADPADVHKSLLRIRERRYASFIP 350 Query: 449 WCPTGFKVGINYQPPTV 499 W P +V ++ + P + Sbjct: 351 WGPASIQVALSKKSPYI 367 >SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharomyces pombe|chr 2|||Manual Length = 903 Score = 33.1 bits (72), Expect = 0.044 Identities = 18/54 (33%), Positives = 29/54 (53%) Frame = -3 Query: 197 RHQVSLELGEVHIQSAVESQRSSDRGDXLSDETIQVGVGWALNVEITAADVIDG 36 R + E + H ++ + Q+SSD + +D T VG+G AL I + D +DG Sbjct: 165 RRVLQAERAKEHRSNSNDKQKSSDNDEDPNDTT--VGIGAALAASILSVDSVDG 216 >SPBC428.07 |meu6||meiotic chromosome segregation protein Meu6|Schizosaccharomyces pombe|chr 2|||Manual Length = 651 Score = 32.3 bits (70), Expect = 0.077 Identities = 21/64 (32%), Positives = 29/64 (45%), Gaps = 2/64 (3%) Frame = +3 Query: 153 ALNVDLTEFQTNLVPYPRIHFPLVT--YAPVISAEKPTMNSFPSPRSQTHASSPPTRW*N 326 A +V++ E +TN +P HFP T AP S EKP S + T+ N Sbjct: 368 AQSVEVPENETNQIPTTEEHFPATTEEVAPA-SEEKPATGPAEESTSTQNVEQASTQNDN 426 Query: 327 ATPV 338 TP+ Sbjct: 427 GTPI 430 >SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 857 Score = 31.1 bits (67), Expect = 0.18 Identities = 24/90 (26%), Positives = 32/90 (35%) Frame = +3 Query: 237 VISAEKPTMNSFPSPRSQTHASSPPTRW*NATPVMASTWLAVCCTVVTSYPRM*TRPSLP 416 VI+ P + P PP P + S VV P + RP++P Sbjct: 509 VITPSVPQPPAAPVVPEAPSVHQPPAA--PVAPEVPSAPQRPAAPVVPEAPSVPQRPAVP 566 Query: 417 SKPSVLSNSSTGVQPVSRSVSTTSHPPWCP 506 P LS V PV+ V + PP P Sbjct: 567 VVPEALSVPQPPVAPVAPEVPSVPQPPVAP 596 Score = 26.2 bits (55), Expect = 5.1 Identities = 28/100 (28%), Positives = 36/100 (36%), Gaps = 3/100 (3%) Frame = +3 Query: 216 PLVTYAPVISAEKPTMNSFPSPRSQTHASSPPTRW*NATPVMA---STWLAVCCTVVTSY 386 P APV+ E P++ P S P R A PV+ S VV Sbjct: 590 PQPPVAPVVP-EAPSVPQPPVAPVAPEVPSVPQR--PAVPVVPEAPSVPQPPAAPVVPEV 646 Query: 387 PRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCP 506 P + RP++P P S PV V + PP P Sbjct: 647 PSVPQRPAVPVVPEAPSVPQPPAAPVVPEVPSVPQPPAVP 686 >SPCC18.03 |||shuttle craft like transcriptional regulator|Schizosaccharomyces pombe|chr 3|||Manual Length = 1077 Score = 30.3 bits (65), Expect = 0.31 Identities = 18/57 (31%), Positives = 23/57 (40%) Frame = +2 Query: 239 HLCREAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAI 409 H C++ H L ACFEP N C H K C L + KD N ++ Sbjct: 351 HTCKKRCHSGLC-----GACFEPINAKCYCG-LHSKTYPCSSLPSPSISKKDENGSV 401 >SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 800 Score = 29.9 bits (64), Expect = 0.41 Identities = 24/87 (27%), Positives = 37/87 (42%) Frame = +3 Query: 234 PVISAEKPTMNSFPSPRSQTHASSPPTRW*NATPVMASTWLAVCCTVVTSYPRM*TRPSL 413 PV PT +S +P + T+ ++ + +TPV ++ CT TS P T + Sbjct: 408 PVTPTVPPTSSS--TPLTTTNCTTSTSVPYTSTPVTSTPLATTNCTTSTSVPY--TSTPV 463 Query: 414 PSKPSVLSNSSTGVQPVSRSVSTTSHP 494 S P +N +T S TS P Sbjct: 464 TSTPLTTTNCTTSTSIPYTSTPVTSTP 490 Score = 29.9 bits (64), Expect = 0.41 Identities = 25/91 (27%), Positives = 42/91 (46%), Gaps = 3/91 (3%) Frame = +3 Query: 225 TYAPVISAEKPTMNSFP---SPRSQTHASSPPTRW*NATPVMASTWLAVCCTVVTSYPRM 395 T PV S+ +S P +P + T+ ++ + +TPV ++ CT TS P Sbjct: 505 TSTPVTSSNYTISSSTPVTSTPVTTTNCTTSTSVLYTSTPVTSTPLATTNCTTSTSVPYT 564 Query: 396 *TRPSLPSKPSVLSNSSTGVQPVSRSVSTTS 488 T P S ++ S++ PV+ + TTS Sbjct: 565 ST-PVTSSNYTISSSTPVTSTPVTTTNCTTS 594 Score = 25.8 bits (54), Expect = 6.7 Identities = 26/104 (25%), Positives = 40/104 (38%), Gaps = 2/104 (1%) Frame = +3 Query: 183 TNLVPYPRIHFPLVTYAPVISAEKPTMNS--FPSPRSQTHASSPPTRW*NATPVMASTWL 356 T VP PL T S P ++ +P + T+ ++ + +TPV ++ Sbjct: 410 TPTVPPTSSSTPLTTTNCTTSTSVPYTSTPVTSTPLATTNCTTSTSVPYTSTPVTSTPLT 469 Query: 357 AVCCTVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTS 488 CT TS P T + S P +N +T S TS Sbjct: 470 TTNCTTSTSIPY--TSTPVTSTPLTTTNCTTSTSVPYTSTPVTS 511 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 29.9 bits (64), Expect = 0.41 Identities = 17/42 (40%), Positives = 21/42 (50%) Frame = +3 Query: 192 VPYPRIHFPLVTYAPVISAEKPTMNSFPSPRSQTHASSPPTR 317 VP P P V+ P S P N+ PSP S + AS+ P R Sbjct: 1239 VPAPSSEAPSVS-TPRSSVPSPHSNASPSPTSSSMASAAPAR 1279 >SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1|||Manual Length = 3227 Score = 28.3 bits (60), Expect = 1.3 Identities = 14/48 (29%), Positives = 23/48 (47%) Frame = -1 Query: 412 SDGRVHILGYDVTTVQHTASHVLAMTGVAFHHLVGGLEACVCDLGDGK 269 S G +LGY ++ A++V+A + V HL+ G D + K Sbjct: 407 SAGLTSLLGYHLSVKTPQATYVVARSIVMLDHLIDGYSMAFPDFSESK 454 >SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|chr 1|||Manual Length = 628 Score = 26.2 bits (55), Expect = 5.1 Identities = 14/32 (43%), Positives = 21/32 (65%), Gaps = 3/32 (9%) Frame = +3 Query: 408 SLPSKPSV---LSNSSTGVQPVSRSVSTTSHP 494 +LP KPS+ +++S V+P S STTS+P Sbjct: 5 TLPPKPSISPSIASSFPTVKPFSSQNSTTSNP 36 >SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosaccharomyces pombe|chr 1|||Manual Length = 157 Score = 25.8 bits (54), Expect = 6.7 Identities = 10/20 (50%), Positives = 11/20 (55%) Frame = -3 Query: 389 WVRRHHGTAYSKPCTCHDGG 330 W R H Y+KPC DGG Sbjct: 16 WRRDHPFGFYAKPCKSSDGG 35 >SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces pombe|chr 1|||Manual Length = 665 Score = 25.4 bits (53), Expect = 8.9 Identities = 13/34 (38%), Positives = 19/34 (55%), Gaps = 2/34 (5%) Frame = +3 Query: 405 PSLPSKPSVLSNSST--GVQPVSRSVSTTSHPPW 500 P PS+P+++SN ST G+Q V V + W Sbjct: 531 PISPSRPALISNISTKKGIQVVGNMVYDPTRLRW 564 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 25.4 bits (53), Expect = 8.9 Identities = 9/23 (39%), Positives = 14/23 (60%) Frame = +3 Query: 198 YPRIHFPLVTYAPVISAEKPTMN 266 +PR FP + P++ AE+ T N Sbjct: 24 FPRFQFPSIVGRPILRAEEKTGN 46 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,163,976 Number of Sequences: 5004 Number of extensions: 67224 Number of successful extensions: 279 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 250 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 279 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 363302114 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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