BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30442 (876 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 145 8e-36 SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 143 3e-35 SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 120 3e-28 SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 102 8e-23 SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 92 8e-20 SPBC16C6.05 |||translation initiation factor |Schizosaccharomyce... 33 0.071 SPCC663.03 |pmd1||leptomycin efflux transporter Pmd1|Schizosacch... 29 0.87 SPAC22F8.10c |sap145||U2 snRNP-associated protein Sap145 |Schizo... 28 1.5 SPBC16E9.13 |ksp1|ppk20|serine/threonine protein kinase Ksp1 |Sc... 28 2.0 SPBPB8B6.03 ||SPAPB8B6.03, SPAPB8B6.03|acetamidase |Schizosaccha... 28 2.0 SPBC3B9.02c |cwf28||splicing factor Cwf28|Schizosaccharomyces po... 27 2.7 SPBC1734.01c ||SPBC337.17c|RNA-binding protein|Schizosaccharomyc... 27 4.6 SPAC2C4.15c |ubx2|ucp13|UBX domain protein Ubx2|Schizosaccharomy... 27 4.6 SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces pom... 27 4.6 SPAC19G12.03 |cda1||chitin deacetylase Cda1|Schizosaccharomyces ... 26 6.1 SPBC16G5.16 |||transcription factor zf-fungal binuclear cluster ... 26 6.1 >SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces pombe|chr 1|||Manual Length = 644 Score = 145 bits (351), Expect = 8e-36 Identities = 68/86 (79%), Positives = 76/86 (88%) Frame = +3 Query: 249 LAGKFELTGIPPAPRGVPQIEVTFDIDANGILNVSAIEKSTNKENKITITNDKGRLSKEE 428 L GKFEL+GIPPAPRGVPQIEVTFD+DANGILNVSA+EK T K KITITNDKGRLSKEE Sbjct: 453 LLGKFELSGIPPAPRGVPQIEVTFDVDANGILNVSALEKGTGKTQKITITNDKGRLSKEE 512 Query: 429 IERMVNEAEKYRNEDDKQKETIQAKN 506 I+RMV+EAEKY+ ED+ + IQAKN Sbjct: 513 IDRMVSEAEKYKAEDEAETSRIQAKN 538 Score = 97.9 bits (233), Expect = 2e-21 Identities = 53/85 (62%), Positives = 55/85 (64%) Frame = +1 Query: 1 GAAVQAAILHGDKSEEVQDXXXXXXXXXXXGIETAGGVMTTLIKRNXXXXXXXXXXXXXY 180 GAAVQAAIL GD SE+ QD GIETAGGVMT LIKRN Y Sbjct: 370 GAAVQAAILVGDTSEKTQDLLLLDVAPLSLGIETAGGVMTPLIKRNTTIPTKKSEVFSTY 429 Query: 181 SDNQPGVLIQVFEGERAMTKDNNLL 255 +DNQPGVLIQVFEGERA TKD NLL Sbjct: 430 ADNQPGVLIQVFEGERARTKDCNLL 454 Score = 47.2 bits (107), Expect = 3e-06 Identities = 20/33 (60%), Positives = 25/33 (75%) Frame = +1 Query: 598 RQVQHTIKWLDSNQLADKEEYEHKQKELEGIYN 696 + V+ TI+WLD N A K+EYE KQKELEG+ N Sbjct: 570 KAVKETIEWLDHNTTAAKDEYEDKQKELEGVAN 602 Score = 44.4 bits (100), Expect = 2e-05 Identities = 22/72 (30%), Positives = 40/72 (55%), Gaps = 1/72 (1%) Frame = +2 Query: 509 LESYCFSMKSTMEDEKLKEKISDSDKQTILDKCNTPSSGWIP-TSWPTRRSMSTSRKNWK 685 LESY +S++++++D LK+K+ SDK+ I DK + W+ + + +K + Sbjct: 540 LESYAYSLRNSLDDPNLKDKVDASDKEAI-DKAVKETIEWLDHNTTAAKDEYEDKQKELE 598 Query: 686 AFTIPIITKMYQ 721 PI+ K+YQ Sbjct: 599 GVANPIMAKIYQ 610 >SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces pombe|chr 3|||Manual Length = 647 Score = 143 bits (347), Expect = 3e-35 Identities = 68/86 (79%), Positives = 75/86 (87%) Frame = +3 Query: 249 LAGKFELTGIPPAPRGVPQIEVTFDIDANGILNVSAIEKSTNKENKITITNDKGRLSKEE 428 L GKFEL+GIPPAPRGVPQIEVTFD+DANGILNVSA+EK T K KITITNDKGRLSKEE Sbjct: 453 LLGKFELSGIPPAPRGVPQIEVTFDVDANGILNVSALEKGTGKTQKITITNDKGRLSKEE 512 Query: 429 IERMVNEAEKYRNEDDKQKETIQAKN 506 I+RMV EAEKY+ ED+ + IQAKN Sbjct: 513 IDRMVAEAEKYKAEDEAESGRIQAKN 538 Score = 99.1 bits (236), Expect = 7e-22 Identities = 53/85 (62%), Positives = 55/85 (64%) Frame = +1 Query: 1 GAAVQAAILHGDKSEEVQDXXXXXXXXXXXGIETAGGVMTTLIKRNXXXXXXXXXXXXXY 180 GAAVQAA+L GD SE+ QD GIETAGGVMT LIKRN Y Sbjct: 370 GAAVQAAVLTGDTSEKTQDLLLLDVAPLSMGIETAGGVMTPLIKRNTTIPTKKSEIFSTY 429 Query: 181 SDNQPGVLIQVFEGERAMTKDNNLL 255 SDNQPGVLIQVFEGERA TKD NLL Sbjct: 430 SDNQPGVLIQVFEGERARTKDCNLL 454 Score = 48.0 bits (109), Expect = 2e-06 Identities = 22/72 (30%), Positives = 43/72 (59%), Gaps = 1/72 (1%) Frame = +2 Query: 509 LESYCFSMKSTMEDEKLKEKISDSDKQTILDKCNTPSSGWIPTSWPTRR-SMSTSRKNWK 685 LESY +S++++++D LK+K+ SDK+T+ DK + W+ ++ + +K + Sbjct: 540 LESYAYSLRNSLDDPNLKDKVDASDKETV-DKAVKETIEWLDSNTTAAKDEFEAKQKELE 598 Query: 686 AFTIPIITKMYQ 721 + PI+ K+YQ Sbjct: 599 SVANPIMAKIYQ 610 Score = 44.8 bits (101), Expect = 2e-05 Identities = 19/33 (57%), Positives = 25/33 (75%) Frame = +1 Query: 598 RQVQHTIKWLDSNQLADKEEYEHKQKELEGIYN 696 + V+ TI+WLDSN A K+E+E KQKELE + N Sbjct: 570 KAVKETIEWLDSNTTAAKDEFEAKQKELESVAN 602 >SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||Manual Length = 663 Score = 120 bits (289), Expect = 3e-28 Identities = 57/87 (65%), Positives = 70/87 (80%), Gaps = 1/87 (1%) Frame = +3 Query: 249 LAGKFELTGIPPAPRGVPQIEVTFDIDANGILNVSAIEKS-TNKENKITITNDKGRLSKE 425 L GKF+L GIPPAPRGVPQIEVTF++DANG+L VSA++KS K K+ I NDKGRLS+E Sbjct: 482 LLGKFDLRGIPPAPRGVPQIEVTFEVDANGVLTVSAVDKSGKGKPEKLVIKNDKGRLSEE 541 Query: 426 EIERMVNEAEKYRNEDDKQKETIQAKN 506 +IERMV EAE++ ED KE I+A+N Sbjct: 542 DIERMVKEAEEFAEEDKILKERIEARN 568 Score = 69.3 bits (162), Expect = 7e-13 Identities = 40/85 (47%), Positives = 47/85 (55%) Frame = +1 Query: 1 GAAVQAAILHGDKSEEVQDXXXXXXXXXXXGIETAGGVMTTLIKRNXXXXXXXXXXXXXY 180 GAAVQA +L G++ + + GIET GGVMT LI RN Sbjct: 401 GAAVQAGVLSGEEGSD--NIVLLDVIPLTLGIETTGGVMTKLIGRNTPIPTRKSQIFSTA 458 Query: 181 SDNQPGVLIQVFEGERAMTKDNNLL 255 DNQ VLIQV+EGER +TKDNNLL Sbjct: 459 VDNQNTVLIQVYEGERTLTKDNNLL 483 Score = 34.7 bits (76), Expect = 0.017 Identities = 21/73 (28%), Positives = 34/73 (46%), Gaps = 3/73 (4%) Frame = +2 Query: 509 LESYCFSMKSTMED-EKLKEKISDSDKQTILDKCNTPSSGWIPTSW--PTRRSMSTSRKN 679 LE+Y +S+K +D E+L K+ DKQ +LD + W+ ++ R+ Sbjct: 570 LENYAYSLKGQFDDDEQLGGKVDPEDKQAVLDAVEDVAE-WLEIHGEDASKEEFEDQRQK 628 Query: 680 WKAFTIPIITKMY 718 A PI K+Y Sbjct: 629 LDAVVHPITQKLY 641 >SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces pombe|chr 1|||Manual Length = 674 Score = 102 bits (244), Expect = 8e-23 Identities = 49/87 (56%), Positives = 65/87 (74%) Frame = +3 Query: 246 QLAGKFELTGIPPAPRGVPQIEVTFDIDANGILNVSAIEKSTNKENKITITNDKGRLSKE 425 +L G F+LTGI PAP+G PQIEV+FD+DA+GI+NVSA +K+TNK++ IT+ G L+ Sbjct: 495 KLIGNFQLTGIAPAPKGQPQIEVSFDVDADGIINVSARDKATNKDSSITVAGSSG-LTDS 553 Query: 426 EIERMVNEAEKYRNEDDKQKETIQAKN 506 EIE MV +AEKYR D +KE I+ N Sbjct: 554 EIEAMVADAEKYRASDMARKEAIENGN 580 Score = 56.0 bits (129), Expect = 7e-09 Identities = 33/87 (37%), Positives = 42/87 (48%) Frame = +1 Query: 1 GAAVQAAILHGDKSEEVQDXXXXXXXXXXXGIETAGGVMTTLIKRNXXXXXXXXXXXXXY 180 GAA+Q +L G V+D GIET GGV T LI RN Sbjct: 417 GAAIQGGVLSG----HVKDLVLLDVTPLSLGIETLGGVFTRLINRNTTIPTRKSQVFSTA 472 Query: 181 SDNQPGVLIQVFEGERAMTKDNNLLVN 261 +D Q V I+VF+GER + +DN L+ N Sbjct: 473 ADGQTAVEIRVFQGERELVRDNKLIGN 499 >SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces pombe|chr 2|||Manual Length = 613 Score = 92.3 bits (219), Expect = 8e-20 Identities = 39/84 (46%), Positives = 60/84 (71%) Frame = +3 Query: 255 GKFELTGIPPAPRGVPQIEVTFDIDANGILNVSAIEKSTNKENKITITNDKGRLSKEEIE 434 G+F+LTGIPP PRG ++E TF++DANGIL V+A+EK+T + I ITN G LS +I+ Sbjct: 460 GEFQLTGIPPMPRGQAELEATFELDANGILKVTAVEKTTGRSAHIEITNSVGHLSSTKIQ 519 Query: 435 RMVNEAEKYRNEDDKQKETIQAKN 506 M+ A+K++ +D + ++AK+ Sbjct: 520 EMIENADKFKQQDKDFAKKLEAKS 543 Score = 39.1 bits (87), Expect = 8e-04 Identities = 26/86 (30%), Positives = 35/86 (40%), Gaps = 1/86 (1%) Frame = +1 Query: 1 GAAVQAAILHGDK-SEEVQDXXXXXXXXXXXGIETAGGVMTTLIKRNXXXXXXXXXXXXX 177 GAAVQAA+L S++ QD G+ G V + RN Sbjct: 374 GAAVQAAVLTNKADSDKTQDLLLLDVVPLSLGVAMEGNVFGVVCPRNTPIPTIKKRTFTT 433 Query: 178 YSDNQPGVLIQVFEGERAMTKDNNLL 255 +DNQ V V++GER +N L Sbjct: 434 VADNQTTVTFPVYQGERVNCAENEPL 459 >SPBC16C6.05 |||translation initiation factor |Schizosaccharomyces pombe|chr 2|||Manual Length = 190 Score = 32.7 bits (71), Expect = 0.071 Identities = 19/66 (28%), Positives = 34/66 (51%), Gaps = 2/66 (3%) Frame = +3 Query: 327 DANGILNVSAIEKSTNKENKITITNDKGRLSKEEIERMVNEAEKYRNEDDKQKE--TIQA 500 D LNVS + S +E +T ++ R+ +EE +RM ++ E K+K T+Q Sbjct: 58 DLENTLNVSGTKDSNAEEQPAKLTKEEKRVEREEAKRMASKVLIKTIERTKRKRVTTVQG 117 Query: 501 KNAWNL 518 +A+ + Sbjct: 118 LDAFGI 123 >SPCC663.03 |pmd1||leptomycin efflux transporter Pmd1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1362 Score = 29.1 bits (62), Expect = 0.87 Identities = 14/24 (58%), Positives = 16/24 (66%) Frame = +3 Query: 405 KGRLSKEEIERMVNEAEKYRNEDD 476 KG LSKEE+ER V +A K N D Sbjct: 525 KGTLSKEELERRVYDAAKLANAYD 548 >SPAC22F8.10c |sap145||U2 snRNP-associated protein Sap145 |Schizosaccharomyces pombe|chr 1|||Manual Length = 601 Score = 28.3 bits (60), Expect = 1.5 Identities = 16/51 (31%), Positives = 26/51 (50%) Frame = +3 Query: 363 KSTNKENKITITNDKGRLSKEEIERMVNEAEKYRNEDDKQKETIQAKNAWN 515 K NK+++ + +K +L +E+ AEK N DDK + T N +N Sbjct: 39 KQKNKKSRNQVRREKKKLLREKTNSGAKLAEK--NSDDKDQLTENNDNLYN 87 >SPBC16E9.13 |ksp1|ppk20|serine/threonine protein kinase Ksp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 474 Score = 27.9 bits (59), Expect = 2.0 Identities = 15/44 (34%), Positives = 22/44 (50%) Frame = +3 Query: 342 LNVSAIEKSTNKENKITITNDKGRLSKEEIERMVNEAEKYRNED 473 L VS ++S K + + K EE+E VNE E++R D Sbjct: 257 LCVSPEKRSLAKTREAVLAVTKWTTDDEELESFVNEEEEFRASD 300 >SPBPB8B6.03 ||SPAPB8B6.03, SPAPB8B6.03|acetamidase |Schizosaccharomyces pombe|chr 2|||Manual Length = 547 Score = 27.9 bits (59), Expect = 2.0 Identities = 14/65 (21%), Positives = 31/65 (47%) Frame = +3 Query: 354 AIEKSTNKENKITITNDKGRLSKEEIERMVNEAEKYRNEDDKQKETIQAKNAWNLTASA* 533 A++ ST + + + +TN +E I ++ + + + ET+ A AW + + Sbjct: 245 ALKPSTGRISYLNVTNSYS--GQELIPSVIGPMARSLKDIEFFTETVIASEAWKIDSKLL 302 Query: 534 SLPWR 548 +PW+ Sbjct: 303 PIPWK 307 >SPBC3B9.02c |cwf28||splicing factor Cwf28|Schizosaccharomyces pombe|chr 2|||Manual Length = 381 Score = 27.5 bits (58), Expect = 2.7 Identities = 13/36 (36%), Positives = 17/36 (47%), Gaps = 1/36 (2%) Frame = +2 Query: 335 RYPQRFRYREVHQQGEQDHHYQRQRSS-LQGRDRAY 439 R P R +YRE H + + H R QGR +Y Sbjct: 320 RDPDRTKYREYHSERRKQHRTDRYSDDYYQGRSYSY 355 >SPBC1734.01c ||SPBC337.17c|RNA-binding protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 682 Score = 26.6 bits (56), Expect = 4.6 Identities = 13/28 (46%), Positives = 16/28 (57%) Frame = +3 Query: 372 NKENKITITNDKGRLSKEEIERMVNEAE 455 NK+ K T D SKEE+E +V E E Sbjct: 529 NKKGKHTQIEDPTAASKEELENLVREDE 556 >SPAC2C4.15c |ubx2|ucp13|UBX domain protein Ubx2|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 26.6 bits (56), Expect = 4.6 Identities = 15/27 (55%), Positives = 15/27 (55%) Frame = -3 Query: 352 ETLRIPLASMSKVTSI*GTPRGAGGIP 272 E L PLA MS TSI G G GG P Sbjct: 76 EVLVDPLADMSAGTSIMGNNFGFGGFP 102 >SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces pombe|chr 2|||Manual Length = 1067 Score = 26.6 bits (56), Expect = 4.6 Identities = 13/54 (24%), Positives = 28/54 (51%) Frame = +3 Query: 348 VSAIEKSTNKENKITITNDKGRLSKEEIERMVNEAEKYRNEDDKQKETIQAKNA 509 V A+ KS +++ ++ G + EEI ++++ E E+D++ E N+ Sbjct: 597 VPALFKSIDQDESDILSERIGAPTAEEISQLLDSVETNEEENDEELEKAMGVNS 650 >SPAC19G12.03 |cda1||chitin deacetylase Cda1|Schizosaccharomyces pombe|chr 1|||Manual Length = 320 Score = 26.2 bits (55), Expect = 6.1 Identities = 9/37 (24%), Positives = 19/37 (51%) Frame = +1 Query: 610 HTIKWLDSNQLADKEEYEHKQKELEGIYNSDNYEDVP 720 H+ +W++ + + EYEH +K ++ I + P Sbjct: 126 HSHRWINYEGVPPETEYEHIKKSVQAIQKASPSNSAP 162 >SPBC16G5.16 |||transcription factor zf-fungal binuclear cluster type |Schizosaccharomyces pombe|chr 2|||Manual Length = 827 Score = 26.2 bits (55), Expect = 6.1 Identities = 13/28 (46%), Positives = 14/28 (50%) Frame = +2 Query: 137 TLPSPLNRLRHSPPTLITNPEYSSKYLR 220 T P RLRH+P I E KYLR Sbjct: 43 TYGQPFKRLRHAPEKYIEFLELRLKYLR 70 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,335,347 Number of Sequences: 5004 Number of extensions: 64377 Number of successful extensions: 305 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 273 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 301 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 438479610 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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