BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30436 (873 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large subunit|... 28 2.0 SPAC1F8.05 |isp3|meu4|sequence orphan|Schizosaccharomyces pombe|... 27 4.6 SPBC2G2.12 |||histidine-tRNA ligase|Schizosaccharomyces pombe|ch... 27 4.6 SPCC14G10.04 |||sequence orphan|Schizosaccharomyces pombe|chr 3|... 26 8.1 SPBC4F6.06 |kin1||microtubule affinity-regulating kinase Kin1 |S... 26 8.1 >SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large subunit|Schizosaccharomyces pombe|chr 2|||Manual Length = 1752 Score = 27.9 bits (59), Expect = 2.0 Identities = 14/23 (60%), Positives = 15/23 (65%), Gaps = 1/23 (4%) Frame = +1 Query: 517 SSPS-QPPKPSYLPRSHSWSPVS 582 SSPS P PSY P S S+SP S Sbjct: 1574 SSPSYSPTSPSYSPTSPSYSPTS 1596 Score = 27.5 bits (58), Expect = 2.6 Identities = 11/22 (50%), Positives = 14/22 (63%) Frame = +1 Query: 517 SSPSQPPKPSYLPRSHSWSPVS 582 SSP P+Y+P S S+SP S Sbjct: 1561 SSPGYSTSPAYMPSSPSYSPTS 1582 Score = 27.1 bits (57), Expect = 3.5 Identities = 15/35 (42%), Positives = 20/35 (57%), Gaps = 1/35 (2%) Frame = +1 Query: 517 SSPS-QPPKPSYLPRSHSWSPVSLL*RSCWPASTP 618 +SPS P PSY P S S+SP S + P+ +P Sbjct: 1581 TSPSYSPTSPSYSPTSPSYSPTSPSYSATSPSYSP 1615 Score = 26.6 bits (56), Expect = 4.6 Identities = 13/23 (56%), Positives = 15/23 (65%), Gaps = 1/23 (4%) Frame = +1 Query: 517 SSPS-QPPKPSYLPRSHSWSPVS 582 +SPS P PSY P S S+SP S Sbjct: 1609 TSPSYSPTSPSYSPTSPSYSPTS 1631 Score = 26.6 bits (56), Expect = 4.6 Identities = 13/23 (56%), Positives = 15/23 (65%), Gaps = 1/23 (4%) Frame = +1 Query: 517 SSPS-QPPKPSYLPRSHSWSPVS 582 +SPS P PSY P S S+SP S Sbjct: 1616 TSPSYSPTSPSYSPTSPSYSPTS 1638 Score = 26.6 bits (56), Expect = 4.6 Identities = 13/23 (56%), Positives = 15/23 (65%), Gaps = 1/23 (4%) Frame = +1 Query: 517 SSPS-QPPKPSYLPRSHSWSPVS 582 +SPS P PSY P S S+SP S Sbjct: 1623 TSPSYSPTSPSYSPTSPSYSPTS 1645 Score = 26.6 bits (56), Expect = 4.6 Identities = 13/23 (56%), Positives = 15/23 (65%), Gaps = 1/23 (4%) Frame = +1 Query: 517 SSPS-QPPKPSYLPRSHSWSPVS 582 +SPS P PSY P S S+SP S Sbjct: 1630 TSPSYSPTSPSYSPTSPSYSPTS 1652 Score = 26.6 bits (56), Expect = 4.6 Identities = 13/23 (56%), Positives = 15/23 (65%), Gaps = 1/23 (4%) Frame = +1 Query: 517 SSPS-QPPKPSYLPRSHSWSPVS 582 +SPS P PSY P S S+SP S Sbjct: 1637 TSPSYSPTSPSYSPTSPSYSPTS 1659 Score = 26.6 bits (56), Expect = 4.6 Identities = 13/23 (56%), Positives = 15/23 (65%), Gaps = 1/23 (4%) Frame = +1 Query: 517 SSPS-QPPKPSYLPRSHSWSPVS 582 +SPS P PSY P S S+SP S Sbjct: 1644 TSPSYSPTSPSYSPTSPSYSPTS 1666 Score = 26.6 bits (56), Expect = 4.6 Identities = 13/23 (56%), Positives = 15/23 (65%), Gaps = 1/23 (4%) Frame = +1 Query: 517 SSPS-QPPKPSYLPRSHSWSPVS 582 +SPS P PSY P S S+SP S Sbjct: 1651 TSPSYSPTSPSYSPTSPSYSPTS 1673 Score = 26.6 bits (56), Expect = 4.6 Identities = 13/23 (56%), Positives = 15/23 (65%), Gaps = 1/23 (4%) Frame = +1 Query: 517 SSPS-QPPKPSYLPRSHSWSPVS 582 +SPS P PSY P S S+SP S Sbjct: 1658 TSPSYSPTSPSYSPTSPSYSPTS 1680 Score = 26.6 bits (56), Expect = 4.6 Identities = 13/23 (56%), Positives = 15/23 (65%), Gaps = 1/23 (4%) Frame = +1 Query: 517 SSPS-QPPKPSYLPRSHSWSPVS 582 +SPS P PSY P S S+SP S Sbjct: 1665 TSPSYSPTSPSYSPTSPSYSPTS 1687 Score = 26.6 bits (56), Expect = 4.6 Identities = 13/23 (56%), Positives = 15/23 (65%), Gaps = 1/23 (4%) Frame = +1 Query: 517 SSPS-QPPKPSYLPRSHSWSPVS 582 +SPS P PSY P S S+SP S Sbjct: 1672 TSPSYSPTSPSYSPTSPSYSPTS 1694 Score = 26.6 bits (56), Expect = 4.6 Identities = 13/23 (56%), Positives = 15/23 (65%), Gaps = 1/23 (4%) Frame = +1 Query: 517 SSPS-QPPKPSYLPRSHSWSPVS 582 +SPS P PSY P S S+SP S Sbjct: 1679 TSPSYSPTSPSYSPTSPSYSPTS 1701 Score = 26.6 bits (56), Expect = 4.6 Identities = 13/23 (56%), Positives = 15/23 (65%), Gaps = 1/23 (4%) Frame = +1 Query: 517 SSPS-QPPKPSYLPRSHSWSPVS 582 +SPS P PSY P S S+SP S Sbjct: 1686 TSPSYSPTSPSYSPTSPSYSPTS 1708 Score = 26.6 bits (56), Expect = 4.6 Identities = 13/23 (56%), Positives = 15/23 (65%), Gaps = 1/23 (4%) Frame = +1 Query: 517 SSPS-QPPKPSYLPRSHSWSPVS 582 +SPS P PSY P S S+SP S Sbjct: 1693 TSPSYSPTSPSYSPTSPSYSPTS 1715 Score = 26.6 bits (56), Expect = 4.6 Identities = 13/23 (56%), Positives = 15/23 (65%), Gaps = 1/23 (4%) Frame = +1 Query: 517 SSPS-QPPKPSYLPRSHSWSPVS 582 +SPS P PSY P S S+SP S Sbjct: 1700 TSPSYSPTSPSYSPTSPSYSPTS 1722 Score = 26.6 bits (56), Expect = 4.6 Identities = 13/23 (56%), Positives = 15/23 (65%), Gaps = 1/23 (4%) Frame = +1 Query: 517 SSPS-QPPKPSYLPRSHSWSPVS 582 +SPS P PSY P S S+SP S Sbjct: 1707 TSPSYSPTSPSYSPTSPSYSPTS 1729 Score = 26.6 bits (56), Expect = 4.6 Identities = 13/23 (56%), Positives = 15/23 (65%), Gaps = 1/23 (4%) Frame = +1 Query: 517 SSPS-QPPKPSYLPRSHSWSPVS 582 +SPS P PSY P S S+SP S Sbjct: 1714 TSPSYSPTSPSYSPTSPSYSPTS 1736 Score = 26.6 bits (56), Expect = 4.6 Identities = 13/23 (56%), Positives = 15/23 (65%), Gaps = 1/23 (4%) Frame = +1 Query: 517 SSPS-QPPKPSYLPRSHSWSPVS 582 +SPS P PSY P S S+SP S Sbjct: 1721 TSPSYSPTSPSYSPTSPSYSPTS 1743 Score = 26.6 bits (56), Expect = 4.6 Identities = 13/23 (56%), Positives = 15/23 (65%), Gaps = 1/23 (4%) Frame = +1 Query: 517 SSPS-QPPKPSYLPRSHSWSPVS 582 +SPS P PSY P S S+SP S Sbjct: 1728 TSPSYSPTSPSYSPTSPSYSPTS 1750 >SPAC1F8.05 |isp3|meu4|sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 182 Score = 26.6 bits (56), Expect = 4.6 Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 3/35 (8%) Frame = +3 Query: 609 KHTETSYEWFAHP---HHEEHYASSGHGWGRSIDD 704 +H + +Y W+ P HH+EH S H W S DD Sbjct: 39 QHQQPTY-WYPPPPPRHHKEH-KKSHHHWEYSSDD 71 >SPBC2G2.12 |||histidine-tRNA ligase|Schizosaccharomyces pombe|chr 2|||Manual Length = 538 Score = 26.6 bits (56), Expect = 4.6 Identities = 14/32 (43%), Positives = 19/32 (59%) Frame = +1 Query: 274 LEPLPEEPKAREAQVESRLVDGVADFLENYVV 369 L+ LP E RE VE L + VAD ++ YV+ Sbjct: 249 LDKLPWEDVRREMVVEKGLKEEVADKIKEYVL 280 >SPCC14G10.04 |||sequence orphan|Schizosaccharomyces pombe|chr 3|||Manual Length = 497 Score = 25.8 bits (54), Expect = 8.1 Identities = 11/34 (32%), Positives = 18/34 (52%) Frame = -1 Query: 135 SLTTPRTLPRMFGSESCSAAGIAHATPITIKTTR 34 S++TP P F S + + + HA P T ++R Sbjct: 251 SVSTPGGTPPRFASPALTVESMVHAAPSTTTSSR 284 >SPBC4F6.06 |kin1||microtubule affinity-regulating kinase Kin1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 891 Score = 25.8 bits (54), Expect = 8.1 Identities = 10/23 (43%), Positives = 13/23 (56%) Frame = -2 Query: 89 LVRQQASPTPRRSPLKRPDTSYR 21 L Q+ PTPR P +P SY+ Sbjct: 624 LETSQSPPTPRTKPSHKPPVSYK 646 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,214,807 Number of Sequences: 5004 Number of extensions: 60220 Number of successful extensions: 228 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 191 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 227 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 436477420 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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