BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30436 (873 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g33494.1 68416.m04279 hypothetical protein 30 1.8 At5g26860.1 68418.m03204 Lon protease homolog 2, mitochondrial a... 29 5.4 At5g13570.1 68418.m01568 MutT/nudix family protein similar to mR... 29 5.4 At4g09400.1 68417.m01549 hypothetical protein similar to At3g453... 29 5.4 At1g51680.2 68414.m05823 4-coumarate--CoA ligase 1 / 4-coumaroyl... 28 7.1 At1g51680.1 68414.m05822 4-coumarate--CoA ligase 1 / 4-coumaroyl... 28 7.1 At4g35800.1 68417.m05087 DNA-directed RNA polymerase II largest ... 28 9.4 At2g35270.1 68415.m04326 DNA-binding protein-related contains Pf... 28 9.4 >At3g33494.1 68416.m04279 hypothetical protein Length = 198 Score = 30.3 bits (65), Expect = 1.8 Identities = 18/46 (39%), Positives = 26/46 (56%) Frame = -2 Query: 524 GDDTQDRGMRAMIFSLAMAKRGRSCLIFFFLPRASSSDLLMPSTAE 387 GDD +R +RA+ FSL + R+ L F +++ LL PS AE Sbjct: 43 GDDRGERSVRALDFSLQGEELLRAGLSLFLQIKSAIGLLLSPSRAE 88 >At5g26860.1 68418.m03204 Lon protease homolog 2, mitochondrial almost identical to Lon protease homolog 2 mitochondrial precursor SP:P93655, GI:1848290 from [Arabidopsis thaliana] Length = 940 Score = 28.7 bits (61), Expect = 5.4 Identities = 14/39 (35%), Positives = 21/39 (53%) Frame = +1 Query: 283 LPEEPKAREAQVESRLVDGVADFLENYVVQFKLPSSAVE 399 +PEEP E+ +V V + +EN+ V+ SSA E Sbjct: 674 VPEEPAVASDPEEAEIVADVGESIENHTVEENTVSSAEE 712 >At5g13570.1 68418.m01568 MutT/nudix family protein similar to mRNA-decapping enzyme [Homo sapiens] GI:23268269; contains Pfam profile PF00293: NUDIX domain Length = 373 Score = 28.7 bits (61), Expect = 5.4 Identities = 22/75 (29%), Positives = 36/75 (48%), Gaps = 2/75 (2%) Frame = +2 Query: 29 KCLVVLMVIGVAWAMPAAEQDSDPNILGSVLGVVKECVDGDVTLCLKEKALRYVE-TLRS 205 +CL+V G +W+ P ++ D + V E DV+ LK + Y+E R Sbjct: 122 RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVSKLLKRE--EYIEFVFRQ 179 Query: 206 KR-EITLVDGVTLDS 247 +R + +V GVT D+ Sbjct: 180 QRVRLYIVAGVTEDT 194 >At4g09400.1 68417.m01549 hypothetical protein similar to At3g45350, At2g14330 Length = 513 Score = 28.7 bits (61), Expect = 5.4 Identities = 20/62 (32%), Positives = 31/62 (50%) Frame = -2 Query: 506 RGMRAMIFSLAMAKRGRSCLIFFFLPRASSSDLLMPSTAEDGSLNCTT*FSKKSATPSTS 327 R +R I S+ A+ GR +F+F SS +++ ED + T F +K+ PST Sbjct: 285 RSIRTAI-SILRARGGRD--LFWFYTGKRSSGIVLTLGCEDRHICSTRTFHRKTTAPSTP 341 Query: 326 LD 321 D Sbjct: 342 DD 343 >At1g51680.2 68414.m05823 4-coumarate--CoA ligase 1 / 4-coumaroyl-CoA synthase 1 (4CL1) identical to SP|Q42524 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1) (4-coumaroyl-CoA synthase 1) {Arabidopsis thaliana} Length = 490 Score = 28.3 bits (60), Expect = 7.1 Identities = 15/50 (30%), Positives = 26/50 (52%), Gaps = 1/50 (2%) Frame = +2 Query: 122 GVVKECVDGDVTLCLKEKALRYVETLRSKREIT-LVDGVTLDSKGYPGLP 268 GVV C+D + ++ + E LR+ E +S E + ++D V + LP Sbjct: 159 GVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVEISPDDVVALP 208 >At1g51680.1 68414.m05822 4-coumarate--CoA ligase 1 / 4-coumaroyl-CoA synthase 1 (4CL1) identical to SP|Q42524 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1) (4-coumaroyl-CoA synthase 1) {Arabidopsis thaliana} Length = 561 Score = 28.3 bits (60), Expect = 7.1 Identities = 15/50 (30%), Positives = 26/50 (52%), Gaps = 1/50 (2%) Frame = +2 Query: 122 GVVKECVDGDVTLCLKEKALRYVETLRSKREIT-LVDGVTLDSKGYPGLP 268 GVV C+D + ++ + E LR+ E +S E + ++D V + LP Sbjct: 159 GVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVEISPDDVVALP 208 >At4g35800.1 68417.m05087 DNA-directed RNA polymerase II largest subunit (RPB205) (RPII) (RPB1) nearly identical to P|P18616 DNA-directed RNA polymerase II largest subunit (EC 2.7.7.6) {Arabidopsis thaliana} Length = 1840 Score = 27.9 bits (59), Expect = 9.4 Identities = 16/35 (45%), Positives = 19/35 (54%), Gaps = 1/35 (2%) Frame = +1 Query: 517 SSPS-QPPKPSYLPRSHSWSPVSLL*RSCWPASTP 618 +SPS P PSY P S S+SP S PA +P Sbjct: 1618 TSPSYSPTSPSYSPTSPSYSPTSPAYSPTSPAYSP 1652 Score = 27.9 bits (59), Expect = 9.4 Identities = 15/42 (35%), Positives = 22/42 (52%), Gaps = 1/42 (2%) Frame = +1 Query: 517 SSPSQPPKPSYLPRSHSWSPVSLL*RSCWPASTP-RPVTNGS 639 +SP P P+Y P S S+SP S P +P P ++G+ Sbjct: 1750 ASPYSPTSPNYSPTSPSYSPTSPSYSPSSPTYSPSSPYSSGA 1791 >At2g35270.1 68415.m04326 DNA-binding protein-related contains Pfam domain PF03479: Domain of unknown function (DUF296), found in AT-hook motifs Pfam:PF02178 Length = 285 Score = 27.9 bits (59), Expect = 9.4 Identities = 12/31 (38%), Positives = 16/31 (51%) Frame = +3 Query: 597 LLASKHTETSYEWFAHPHHEEHYASSGHGWG 689 ++A+ T +YE HEEH S G G G Sbjct: 212 VMAASFTNVAYERLPLDEHEEHLQSGGGGGG 242 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 17,566,862 Number of Sequences: 28952 Number of extensions: 348924 Number of successful extensions: 998 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 938 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 992 length of database: 12,070,560 effective HSP length: 80 effective length of database: 9,754,400 effective search space used: 2048424000 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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