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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS30436
         (873 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g33494.1 68416.m04279 hypothetical protein                          30   1.8  
At5g26860.1 68418.m03204 Lon protease homolog 2, mitochondrial a...    29   5.4  
At5g13570.1 68418.m01568 MutT/nudix family protein similar to mR...    29   5.4  
At4g09400.1 68417.m01549 hypothetical protein similar to At3g453...    29   5.4  
At1g51680.2 68414.m05823 4-coumarate--CoA ligase 1 / 4-coumaroyl...    28   7.1  
At1g51680.1 68414.m05822 4-coumarate--CoA ligase 1 / 4-coumaroyl...    28   7.1  
At4g35800.1 68417.m05087 DNA-directed RNA polymerase II largest ...    28   9.4  
At2g35270.1 68415.m04326 DNA-binding protein-related contains Pf...    28   9.4  

>At3g33494.1 68416.m04279 hypothetical protein
          Length = 198

 Score = 30.3 bits (65), Expect = 1.8
 Identities = 18/46 (39%), Positives = 26/46 (56%)
 Frame = -2

Query: 524 GDDTQDRGMRAMIFSLAMAKRGRSCLIFFFLPRASSSDLLMPSTAE 387
           GDD  +R +RA+ FSL   +  R+ L  F   +++   LL PS AE
Sbjct: 43  GDDRGERSVRALDFSLQGEELLRAGLSLFLQIKSAIGLLLSPSRAE 88


>At5g26860.1 68418.m03204 Lon protease homolog 2, mitochondrial
           almost identical to Lon protease homolog 2
           mitochondrial precursor SP:P93655, GI:1848290 from
           [Arabidopsis thaliana]
          Length = 940

 Score = 28.7 bits (61), Expect = 5.4
 Identities = 14/39 (35%), Positives = 21/39 (53%)
 Frame = +1

Query: 283 LPEEPKAREAQVESRLVDGVADFLENYVVQFKLPSSAVE 399
           +PEEP       E+ +V  V + +EN+ V+    SSA E
Sbjct: 674 VPEEPAVASDPEEAEIVADVGESIENHTVEENTVSSAEE 712


>At5g13570.1 68418.m01568 MutT/nudix family protein similar to
           mRNA-decapping enzyme [Homo sapiens] GI:23268269;
           contains Pfam profile PF00293: NUDIX domain
          Length = 373

 Score = 28.7 bits (61), Expect = 5.4
 Identities = 22/75 (29%), Positives = 36/75 (48%), Gaps = 2/75 (2%)
 Frame = +2

Query: 29  KCLVVLMVIGVAWAMPAAEQDSDPNILGSVLGVVKECVDGDVTLCLKEKALRYVE-TLRS 205
           +CL+V    G +W+ P  ++  D       +  V E    DV+  LK +   Y+E   R 
Sbjct: 122 RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVSKLLKRE--EYIEFVFRQ 179

Query: 206 KR-EITLVDGVTLDS 247
           +R  + +V GVT D+
Sbjct: 180 QRVRLYIVAGVTEDT 194


>At4g09400.1 68417.m01549 hypothetical protein similar to At3g45350,
           At2g14330
          Length = 513

 Score = 28.7 bits (61), Expect = 5.4
 Identities = 20/62 (32%), Positives = 31/62 (50%)
 Frame = -2

Query: 506 RGMRAMIFSLAMAKRGRSCLIFFFLPRASSSDLLMPSTAEDGSLNCTT*FSKKSATPSTS 327
           R +R  I S+  A+ GR   +F+F     SS +++    ED  +  T  F +K+  PST 
Sbjct: 285 RSIRTAI-SILRARGGRD--LFWFYTGKRSSGIVLTLGCEDRHICSTRTFHRKTTAPSTP 341

Query: 326 LD 321
            D
Sbjct: 342 DD 343


>At1g51680.2 68414.m05823 4-coumarate--CoA ligase 1 /
           4-coumaroyl-CoA synthase 1 (4CL1) identical to SP|Q42524
           4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1)
           (4-coumaroyl-CoA synthase 1) {Arabidopsis thaliana}
          Length = 490

 Score = 28.3 bits (60), Expect = 7.1
 Identities = 15/50 (30%), Positives = 26/50 (52%), Gaps = 1/50 (2%)
 Frame = +2

Query: 122 GVVKECVDGDVTLCLKEKALRYVETLRSKREIT-LVDGVTLDSKGYPGLP 268
           GVV  C+D + ++ + E  LR+ E  +S  E + ++D V +       LP
Sbjct: 159 GVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVEISPDDVVALP 208


>At1g51680.1 68414.m05822 4-coumarate--CoA ligase 1 /
           4-coumaroyl-CoA synthase 1 (4CL1) identical to SP|Q42524
           4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1)
           (4-coumaroyl-CoA synthase 1) {Arabidopsis thaliana}
          Length = 561

 Score = 28.3 bits (60), Expect = 7.1
 Identities = 15/50 (30%), Positives = 26/50 (52%), Gaps = 1/50 (2%)
 Frame = +2

Query: 122 GVVKECVDGDVTLCLKEKALRYVETLRSKREIT-LVDGVTLDSKGYPGLP 268
           GVV  C+D + ++ + E  LR+ E  +S  E + ++D V +       LP
Sbjct: 159 GVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVEISPDDVVALP 208


>At4g35800.1 68417.m05087 DNA-directed RNA polymerase II largest
            subunit (RPB205) (RPII) (RPB1) nearly identical to
            P|P18616 DNA-directed RNA polymerase II largest subunit
            (EC 2.7.7.6) {Arabidopsis thaliana}
          Length = 1840

 Score = 27.9 bits (59), Expect = 9.4
 Identities = 16/35 (45%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
 Frame = +1

Query: 517  SSPS-QPPKPSYLPRSHSWSPVSLL*RSCWPASTP 618
            +SPS  P  PSY P S S+SP S       PA +P
Sbjct: 1618 TSPSYSPTSPSYSPTSPSYSPTSPAYSPTSPAYSP 1652



 Score = 27.9 bits (59), Expect = 9.4
 Identities = 15/42 (35%), Positives = 22/42 (52%), Gaps = 1/42 (2%)
 Frame = +1

Query: 517  SSPSQPPKPSYLPRSHSWSPVSLL*RSCWPASTP-RPVTNGS 639
            +SP  P  P+Y P S S+SP S       P  +P  P ++G+
Sbjct: 1750 ASPYSPTSPNYSPTSPSYSPTSPSYSPSSPTYSPSSPYSSGA 1791


>At2g35270.1 68415.m04326 DNA-binding protein-related contains Pfam
           domain PF03479: Domain of unknown function (DUF296),
           found in AT-hook motifs Pfam:PF02178
          Length = 285

 Score = 27.9 bits (59), Expect = 9.4
 Identities = 12/31 (38%), Positives = 16/31 (51%)
 Frame = +3

Query: 597 LLASKHTETSYEWFAHPHHEEHYASSGHGWG 689
           ++A+  T  +YE      HEEH  S G G G
Sbjct: 212 VMAASFTNVAYERLPLDEHEEHLQSGGGGGG 242


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,566,862
Number of Sequences: 28952
Number of extensions: 348924
Number of successful extensions: 998
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 938
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 992
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 2048424000
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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