BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30433 (814 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC11C11.09c |rpl502|rpl5-2, rpl5b|60S ribosomal protein L5|Sch... 130 2e-31 SPAC3H5.12c |rpl501|rpl5-1, rpl5|60S ribosomal protein L5|Schizo... 130 2e-31 SPBC28E12.06c |lvs1|SPBC3H7.16|beige protein homolog|Schizosacch... 28 1.8 SPAC328.06 |ubp2||ubiquitin C-terminal hydrolase Ubp2|Schizosacc... 27 2.4 SPAC1610.01 ||SPAC17A5.17|conserved fungal protein|Schizosacchar... 26 7.3 SPAC22F3.12c |rgs1||regulator of G-protein signaling Rgs1|Schizo... 25 9.7 >SPBC11C11.09c |rpl502|rpl5-2, rpl5b|60S ribosomal protein L5|Schizosaccharomyces pombe|chr 2|||Manual Length = 294 Score = 130 bits (314), Expect = 2e-31 Identities = 55/85 (64%), Positives = 68/85 (80%) Frame = -2 Query: 762 FVKVVKNKQYFKRYQVKFKRRREGKTXYYARKRLVVQDKNKYNTPKYRLIVRLSNKDVTC 583 F+K VK+ YF RYQ K++RRREGKT YYARKRL+ Q KNKYN PKYRL+VR SN+ VTC Sbjct: 3 FIKAVKSSPYFSRYQTKYRRRREGKTDYYARKRLIAQAKNKYNAPKYRLVVRFSNRFVTC 62 Query: 582 QVAYSRIEGDHIVCAAYSHELPRYG 508 Q+ SR+ GD+++ A+S ELPRYG Sbjct: 63 QIVSSRVNGDYVLAHAHSSELPRYG 87 Score = 70.1 bits (164), Expect = 3e-13 Identities = 38/104 (36%), Positives = 54/104 (51%) Frame = -1 Query: 508 VKVGLTNYAAAYSTGXXXXXXXXXXXXXXXXXXXXXXXXXDEYNVEPVDNGPGAFRCYLD 329 +K GL N+ AAY+TG + E +++GP F+ +LD Sbjct: 88 IKWGLANWTAAYATGLLVARRALAKVGLADKYEGVTEPEGEFELTEAIEDGPRPFKVFLD 147 Query: 328 VGLARTTTGARVFGAMKGAVDGGLKFLIPSKDSPRYDAESKKFN 197 VGL RT+TG+RVFGAMKGA DGGL P +D E+++ + Sbjct: 148 VGLKRTSTGSRVFGAMKGASDGGLFIPHSPNRFPGFDIETEELD 191 Score = 25.4 bits (53), Expect = 9.7 Identities = 11/19 (57%), Positives = 13/19 (68%) Frame = -2 Query: 192 EVHRAHIFGLHVAEYMRSL 136 E R +I+G HVAEYM L Sbjct: 193 ETLRKYIYGGHVAEYMEML 211 >SPAC3H5.12c |rpl501|rpl5-1, rpl5|60S ribosomal protein L5|Schizosaccharomyces pombe|chr 1|||Manual Length = 294 Score = 130 bits (314), Expect = 2e-31 Identities = 55/85 (64%), Positives = 68/85 (80%) Frame = -2 Query: 762 FVKVVKNKQYFKRYQVKFKRRREGKTXYYARKRLVVQDKNKYNTPKYRLIVRLSNKDVTC 583 F+K VK+ YF RYQ K++RRREGKT YYARKRL+ Q KNKYN PKYRL+VR SN+ VTC Sbjct: 3 FIKAVKSSPYFSRYQTKYRRRREGKTDYYARKRLIAQAKNKYNAPKYRLVVRFSNRFVTC 62 Query: 582 QVAYSRIEGDHIVCAAYSHELPRYG 508 Q+ SR+ GD+++ A+S ELPRYG Sbjct: 63 QIVSSRVNGDYVLAHAHSSELPRYG 87 Score = 70.1 bits (164), Expect = 3e-13 Identities = 38/104 (36%), Positives = 54/104 (51%) Frame = -1 Query: 508 VKVGLTNYAAAYSTGXXXXXXXXXXXXXXXXXXXXXXXXXDEYNVEPVDNGPGAFRCYLD 329 +K GL N+ AAY+TG + E +++GP F+ +LD Sbjct: 88 IKWGLANWTAAYATGLLVARRALAKVGLADKYEGVTEPEGEFELTEAIEDGPRPFKVFLD 147 Query: 328 VGLARTTTGARVFGAMKGAVDGGLKFLIPSKDSPRYDAESKKFN 197 VGL RT+TG+RVFGAMKGA DGGL P +D E+++ + Sbjct: 148 VGLKRTSTGSRVFGAMKGASDGGLFIPHSPNRFPGFDIETEELD 191 Score = 25.4 bits (53), Expect = 9.7 Identities = 11/19 (57%), Positives = 13/19 (68%) Frame = -2 Query: 192 EVHRAHIFGLHVAEYMRSL 136 E R +I+G HVAEYM L Sbjct: 193 ETLRKYIYGGHVAEYMEML 211 >SPBC28E12.06c |lvs1|SPBC3H7.16|beige protein homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 2609 Score = 27.9 bits (59), Expect = 1.8 Identities = 15/46 (32%), Positives = 25/46 (54%), Gaps = 3/46 (6%) Frame = +1 Query: 640 FVLVLN---NEAFTSIIXSFPFTTPLEFYLVPLEVLFVLHNFNEPI 768 F L LN +E T ++ FPF +P++ E+ +LHN +P+ Sbjct: 1136 FELTLNYDFSELLTKVV--FPFLSPVDTITAEQEMRDLLHNIRQPL 1179 >SPAC328.06 |ubp2||ubiquitin C-terminal hydrolase Ubp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1141 Score = 27.5 bits (58), Expect = 2.4 Identities = 18/65 (27%), Positives = 30/65 (46%), Gaps = 5/65 (7%) Frame = -3 Query: 188 YTGLTSLVCMLLNT*EVLNQDDEDSFPRGNFSKYIKTRRSLQ-----MLFESHLQESPWK 24 +T TSL L N +Q + P G F +++ R +Q +LFE H + + Sbjct: 185 FTTFTSLELFLRNVLVHNDQRAISAAPEGTFERHVGKGRQIQSLMKSLLFEYHHENVNYV 244 Query: 23 PSVRD 9 P++ D Sbjct: 245 PTIAD 249 >SPAC1610.01 ||SPAC17A5.17|conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 217 Score = 25.8 bits (54), Expect = 7.3 Identities = 14/36 (38%), Positives = 20/36 (55%) Frame = -3 Query: 131 QDDEDSFPRGNFSKYIKTRRSLQMLFESHLQESPWK 24 + DE+SFP G + T +S +LF+ Q S WK Sbjct: 41 RSDEESFPLGKLITLLVTSQSF-ILFDEE-QNSGWK 74 >SPAC22F3.12c |rgs1||regulator of G-protein signaling Rgs1|Schizosaccharomyces pombe|chr 1|||Manual Length = 481 Score = 25.4 bits (53), Expect = 9.7 Identities = 8/23 (34%), Positives = 18/23 (78%) Frame = -2 Query: 213 NPKSSMAEVHRAHIFGLHVAEYM 145 +PK S+++V + +FG+ +AE++ Sbjct: 240 SPKDSLSKVTKYQMFGIDIAEWL 262 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,474,246 Number of Sequences: 5004 Number of extensions: 71962 Number of successful extensions: 194 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 180 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 192 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 396433620 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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