Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30428
(510 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g07070.1 68414.m00753 60S ribosomal protein L35a (RPL35aA) si... 82 2e-16
At1g74270.1 68414.m08601 60S ribosomal protein L35a (RPL35aC) si... 81 3e-16
At3g55750.1 68416.m06194 60S ribosomal protein L35a (RPL35aD) ri... 80 1e-15
At1g41880.1 68414.m04836 60S ribosomal protein L35a (RPL35aB) id... 80 1e-15
At3g05180.1 68416.m00565 GDSL-motif lipase/hydrolase family prot... 31 0.60
At2g04350.2 68415.m00434 long-chain-fatty-acid--CoA ligase famil... 28 3.2
At2g04350.1 68415.m00433 long-chain-fatty-acid--CoA ligase famil... 28 3.2
At4g21130.1 68417.m03055 transducin family protein / WD-40 repea... 27 5.5
At5g51195.1 68418.m06348 hypothetical protein 27 7.3
At5g53660.1 68418.m06665 expressed protein 27 9.7
At5g45160.1 68418.m05544 root hair defective 3 GTP-binding (RHD3... 27 9.7
At1g20130.1 68414.m02518 family II extracellular lipase, putativ... 27 9.7
>At1g07070.1 68414.m00753 60S ribosomal protein L35a (RPL35aA)
similar to ribosomal protein L35a GI:57118 from [Rattus
norvegicus]
Length = 112
Score = 82.2 bits (194), Expect = 2e-16
Identities = 40/74 (54%), Positives = 48/74 (64%)
Frame = +1
Query: 250 SLYAGKHCVYVYRAKKRTPIPGGPRGKKTKLRAIWGKVTRPHGNSGSVRAKFKSNLPAQA 429
S Y GK Y+Y+AK + + R IWGKVTRPHGNSG VRAKF SNLP ++
Sbjct: 47 SWYKGKRMAYIYKAKTKK--------NGSHYRCIWGKVTRPHGNSGVVRAKFTSNLPPKS 98
Query: 430 MGHRIRVMLYPSRI 471
MG R+RV +YPS I
Sbjct: 99 MGSRVRVFMYPSNI 112
Score = 37.1 bits (82), Expect = 0.007
Identities = 15/29 (51%), Positives = 20/29 (68%)
Frame = +2
Query: 143 RLYAKAVFTGYKRGLRNQHENTALLKVEG 229
RLY + GYKR NQ+ NT+L++VEG
Sbjct: 11 RLYVRGTILGYKRSKSNQYPNTSLVQVEG 39
>At1g74270.1 68414.m08601 60S ribosomal protein L35a (RPL35aC)
similar to ribosomal protein L33B GB:NP_014877 from
[Saccharomyces cerevisiae]
Length = 112
Score = 81.4 bits (192), Expect = 3e-16
Identities = 39/72 (54%), Positives = 47/72 (65%)
Frame = +1
Query: 256 YAGKHCVYVYRAKKRTPIPGGPRGKKTKLRAIWGKVTRPHGNSGSVRAKFKSNLPAQAMG 435
Y GK Y+Y+AK + + R IWGKVTRPHGNSG VRAKF SNLP ++MG
Sbjct: 49 YKGKRMAYIYKAKTKK--------NGSHYRCIWGKVTRPHGNSGVVRAKFTSNLPPKSMG 100
Query: 436 HRIRVMLYPSRI 471
R+RV +YPS I
Sbjct: 101 SRVRVFMYPSNI 112
Score = 37.5 bits (83), Expect = 0.005
Identities = 14/35 (40%), Positives = 22/35 (62%)
Frame = +2
Query: 143 RLYAKAVFTGYKRGLRNQHENTALLKVEGAKDRND 247
RLY + GYKR NQ+ NT+L+++EG + +
Sbjct: 11 RLYVRGTILGYKRSKSNQYPNTSLVQIEGVNTQEE 45
>At3g55750.1 68416.m06194 60S ribosomal protein L35a (RPL35aD)
ribosomal protein L35a.e.c15, Saccharomyces cerevisiae,
PIR:S44069
Length = 111
Score = 79.8 bits (188), Expect = 1e-15
Identities = 38/72 (52%), Positives = 47/72 (65%)
Frame = +1
Query: 256 YAGKHCVYVYRAKKRTPIPGGPRGKKTKLRAIWGKVTRPHGNSGSVRAKFKSNLPAQAMG 435
Y GK Y+Y+AK + + R IWGKVTRPHGNSG VR+KF SNLP ++MG
Sbjct: 48 YKGKRLAYIYKAKTKK--------NGSHYRCIWGKVTRPHGNSGVVRSKFTSNLPPKSMG 99
Query: 436 HRIRVMLYPSRI 471
R+RV +YPS I
Sbjct: 100 ARVRVFMYPSNI 111
Score = 37.1 bits (82), Expect = 0.007
Identities = 14/35 (40%), Positives = 22/35 (62%)
Frame = +2
Query: 143 RLYAKAVFTGYKRGLRNQHENTALLKVEGAKDRND 247
RLY + GYKR NQ+ NT+L+++EG + +
Sbjct: 10 RLYVRGTVLGYKRSKSNQYPNTSLVQIEGVNTQEE 44
>At1g41880.1 68414.m04836 60S ribosomal protein L35a (RPL35aB)
identical to GB:CAB81600 from [Arabidopsis thaliana]
Length = 111
Score = 79.8 bits (188), Expect = 1e-15
Identities = 38/72 (52%), Positives = 47/72 (65%)
Frame = +1
Query: 256 YAGKHCVYVYRAKKRTPIPGGPRGKKTKLRAIWGKVTRPHGNSGSVRAKFKSNLPAQAMG 435
Y GK Y+Y+AK + + R IWGKVTRPHGNSG VR+KF SNLP ++MG
Sbjct: 48 YKGKRLAYIYKAKTKK--------NGSHYRCIWGKVTRPHGNSGVVRSKFTSNLPPKSMG 99
Query: 436 HRIRVMLYPSRI 471
R+RV +YPS I
Sbjct: 100 ARVRVFMYPSNI 111
Score = 37.5 bits (83), Expect = 0.005
Identities = 14/35 (40%), Positives = 22/35 (62%)
Frame = +2
Query: 143 RLYAKAVFTGYKRGLRNQHENTALLKVEGAKDRND 247
RLY + GYKR NQ+ NT+L+++EG + +
Sbjct: 10 RLYVRGTVLGYKRSKSNQYPNTSLIQIEGVNTQEE 44
>At3g05180.1 68416.m00565 GDSL-motif lipase/hydrolase family protein
similar to early nodulin ENOD8 [Medicago sativa]
GI:304037, elicitor-induced glycoprotein iEP4 [Daucus
carota] GI:1911765, lanatoside 15'-O-acetylesterase
[Digitalis lanata] GI:3688284; contains InterPro Entry
IPR001087 Lipolytic enzyme, G-D-S-L family
Length = 379
Score = 30.7 bits (66), Expect = 0.60
Identities = 18/62 (29%), Positives = 31/62 (50%)
Frame = -1
Query: 396 SDTARVAMWAGHLAPDSTQLGFFATGTSGNWCPLLSSVHIDAMLASIKTASLRSFAPSTL 217
SDT ++ G L S ++ FF + TSG +C + +D ++ +I LR + S
Sbjct: 45 SDTGELSSGLGFLPQPSYEITFFRSPTSGRFCN--GRLIVDFLMEAIDRPYLRPYLDSIS 102
Query: 216 RR 211
R+
Sbjct: 103 RQ 104
>At2g04350.2 68415.m00434 long-chain-fatty-acid--CoA ligase family
protein / long-chain acyl-CoA synthetase family protein
(LACS8) similar to LACS 4 [SP|O35547] from Rattus
norvegicus, LACS 4 [SP|O60488] from Homo sapiens;
contains Pfam HMM hit: AMP-binding enzymes PF00501
Length = 720
Score = 28.3 bits (60), Expect = 3.2
Identities = 16/47 (34%), Positives = 23/47 (48%)
Frame = -2
Query: 497 FL*LAEGS*ILEGYSITRILCPIAWAGRLDLNLARTLPELPCGRVTL 357
F+ + GS I +GY +T ++ D + R P LPCG V L
Sbjct: 463 FINICMGSPIGQGYGLTETCAGATFSEWDDPAVGRVGPPLPCGYVKL 509
>At2g04350.1 68415.m00433 long-chain-fatty-acid--CoA ligase family
protein / long-chain acyl-CoA synthetase family protein
(LACS8) similar to LACS 4 [SP|O35547] from Rattus
norvegicus, LACS 4 [SP|O60488] from Homo sapiens;
contains Pfam HMM hit: AMP-binding enzymes PF00501
Length = 720
Score = 28.3 bits (60), Expect = 3.2
Identities = 16/47 (34%), Positives = 23/47 (48%)
Frame = -2
Query: 497 FL*LAEGS*ILEGYSITRILCPIAWAGRLDLNLARTLPELPCGRVTL 357
F+ + GS I +GY +T ++ D + R P LPCG V L
Sbjct: 463 FINICMGSPIGQGYGLTETCAGATFSEWDDPAVGRVGPPLPCGYVKL 509
>At4g21130.1 68417.m03055 transducin family protein / WD-40 repeat
family protein contains 6 WD-40 repeats (PF00400); some
similarity to a group of proteins with homology to
mammalian apoptosis regulators identified in zebrafish
(PUBMED:10917738)Apaf-1(gi:7677507)
Length = 537
Score = 27.5 bits (58), Expect = 5.5
Identities = 18/57 (31%), Positives = 30/57 (52%), Gaps = 1/57 (1%)
Frame = -1
Query: 453 HHSYSVS-HSLGREVRLELGSDTARVAMWAGHLAPDSTQLGFFATGTSGNWCPLLSS 286
H S +S +LGRE L +G D G + P+ST+L + A+ ++ C ++S
Sbjct: 304 HQSELLSIDALGRERVLSVGRDRTMQLYKVGIVVPESTRLIYRASESNFECCCFVNS 360
>At5g51195.1 68418.m06348 hypothetical protein
Length = 267
Score = 27.1 bits (57), Expect = 7.3
Identities = 13/39 (33%), Positives = 18/39 (46%)
Frame = -1
Query: 417 EVRLELGSDTARVAMWAGHLAPDSTQLGFFATGTSGNWC 301
E++ E+ S+ W D T FFA+ SG WC
Sbjct: 209 EIQAEMESEDVAATFWID----DETHDDFFASSKSGWWC 243
>At5g53660.1 68418.m06665 expressed protein
Length = 365
Score = 26.6 bits (56), Expect = 9.7
Identities = 15/60 (25%), Positives = 28/60 (46%), Gaps = 1/60 (1%)
Frame = +1
Query: 322 RGKKTKLRAIWGKVTRPHGNSGSVRAKFKSNLPAQAMGHRIR-VMLYPSRI*EPSANYKN 498
RG+ + + +RP+ N GSV+ + LP + I+ L P + +NY++
Sbjct: 140 RGRPRSRKHVEPPYSRPNNNGGSVKNRDLKKLPQKLSSSSIKDKTLEPMEVSSSISNYRD 199
>At5g45160.1 68418.m05544 root hair defective 3 GTP-binding (RHD3)
family protein contains Pfam profile: PF05879 root hair
defective 3 GTP-binding protein (RHD3) family
Length = 834
Score = 26.6 bits (56), Expect = 9.7
Identities = 12/44 (27%), Positives = 22/44 (50%)
Frame = +1
Query: 274 VYVYRAKKRTPIPGGPRGKKTKLRAIWGKVTRPHGNSGSVRAKF 405
++V R K +TPI R + ++ IW V +P + + +F
Sbjct: 167 LFVIRDKTKTPIELLERALREDIQKIWDSVRKPEAHKNTPLNEF 210
>At1g20130.1 68414.m02518 family II extracellular lipase, putative
contains Pfam profile PF00657: GDSL-like
Lipase/Acylhydrolase; similar to EXL3 (PMID:11431566)
Length = 1006
Score = 26.6 bits (56), Expect = 9.7
Identities = 15/52 (28%), Positives = 20/52 (38%)
Frame = +1
Query: 295 KRTPIPGGPRGKKTKLRAIWGKVTRPHGNSGSVRAKFKSNLPAQAMGHRIRV 450
K P P P K +G GN+ ++ K KSN M + RV
Sbjct: 132 KPAPSPPKPENKTIPAVFFFGDSVFDTGNNNNLETKIKSNYRPYGMDFKFRV 183
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,807,263
Number of Sequences: 28952
Number of extensions: 232306
Number of successful extensions: 662
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 629
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 658
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 917929344
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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