BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30427 (461 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC3G6.01 |hrp3||ATP-dependent DNA helicase Hrp3|Schizosaccharo... 28 0.80 SPCC1235.10c |sec6||exocyst complex subunit Sec6|Schizosaccharom... 28 0.80 SPAC17C9.13c |cut8||tethering factor for nuclear proteasome Cut8... 27 1.1 SPAC19B12.08 |||peptidase family C54|Schizosaccharomyces pombe|c... 26 2.4 SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 25 4.3 SPAC22G7.10 |||mRNA cleavage and polyadenylation specificity fac... 25 4.3 SPCC191.09c |gst1||glutathione S-transferase Gst1|Schizosaccharo... 25 4.3 SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces pomb... 25 5.6 SPCC162.12 ||SPCC1753.06c|sequence orphan|Schizosaccharomyces po... 25 5.6 >SPAC3G6.01 |hrp3||ATP-dependent DNA helicase Hrp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 1388 Score = 27.9 bits (59), Expect = 0.80 Identities = 11/33 (33%), Positives = 20/33 (60%) Frame = +3 Query: 138 YMWNEFFKHIDIEPSNAHVLDGNASDLVVECRR 236 + +N+ K+ D+ +AHV+ A+DLV R+ Sbjct: 1015 HRYNDIVKYADLTTQDAHVIKKIAADLVTASRK 1047 >SPCC1235.10c |sec6||exocyst complex subunit Sec6|Schizosaccharomyces pombe|chr 3|||Manual Length = 730 Score = 27.9 bits (59), Expect = 0.80 Identities = 12/30 (40%), Positives = 18/30 (60%) Frame = +3 Query: 6 TPLGMYKRLIEFHKEGKLSFKYVTTFNMDE 95 TP +Y LI FH+E + F+ F++DE Sbjct: 321 TPAAVYLYLINFHREYRKFFEENAPFSVDE 350 >SPAC17C9.13c |cut8||tethering factor for nuclear proteasome Cut8|Schizosaccharomyces pombe|chr 1|||Manual Length = 262 Score = 27.5 bits (58), Expect = 1.1 Identities = 15/31 (48%), Positives = 19/31 (61%) Frame = +2 Query: 350 VKTLAYDTLDANKRFFDNDISKVPRQALTVG 442 ++TL+Y + K FD DISK RQ L VG Sbjct: 1 METLSYSQIKKRKADFDEDISKRARQ-LPVG 30 >SPAC19B12.08 |||peptidase family C54|Schizosaccharomyces pombe|chr 1|||Manual Length = 320 Score = 26.2 bits (55), Expect = 2.4 Identities = 18/68 (26%), Positives = 32/68 (47%), Gaps = 4/68 (5%) Frame = +3 Query: 81 FNMDEYVGLPRDHPESYHYY--MWNEFFKHIDIEPSN-AHVLDGNASDLVVECRRFEKL- 248 F + +VG+ P S HY+ N++F ++D ++ AH AS+ ++ Sbjct: 201 FEIRSFVGITGGRPRSAHYFYARQNQYFFYLDPHCTHFAHTTTQPASEETFHSATLRRVA 260 Query: 249 IQRLAECI 272 IQ L C+ Sbjct: 261 IQDLDPCM 268 >SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma subunit|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 25.4 bits (53), Expect = 4.3 Identities = 18/61 (29%), Positives = 28/61 (45%) Frame = -1 Query: 404 RCQRTACWRPGCRRPGSSLASETPARSPVR*TRCDRPDRYLR*TNALRQPLYEFLEASTL 225 +C C RPGC R SS + P P C+ P +R + + P ++ L A+ L Sbjct: 72 KCSNEECPRPGCYRSYSSNKEDHP---PCE--ICNSPMNLVRHVSFVDCPGHDILMATML 126 Query: 224 D 222 + Sbjct: 127 N 127 >SPAC22G7.10 |||mRNA cleavage and polyadenylation specificity factor complex subunit, Fip1 homolog |Schizosaccharomyces pombe|chr 1|||Manual Length = 344 Score = 25.4 bits (53), Expect = 4.3 Identities = 14/34 (41%), Positives = 17/34 (50%) Frame = +2 Query: 230 STLRETHTEAGGVHLFIGGIGPDGHIAFNEPGSS 331 S THT GGVH G P+ ++ N P SS Sbjct: 229 SNYNTTHTSGGGVH--SGAATPNAYVN-NNPSSS 259 >SPCC191.09c |gst1||glutathione S-transferase Gst1|Schizosaccharomyces pombe|chr 3|||Manual Length = 229 Score = 25.4 bits (53), Expect = 4.3 Identities = 8/17 (47%), Positives = 12/17 (70%) Frame = +3 Query: 81 FNMDEYVGLPRDHPESY 131 ++ + + LPRDHPE Y Sbjct: 82 YDTERKISLPRDHPEYY 98 >SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 1207 Score = 25.0 bits (52), Expect = 5.6 Identities = 7/19 (36%), Positives = 13/19 (68%) Frame = +2 Query: 44 QGREIIFQICDHFQHGRVC 100 + R+++F +C H + RVC Sbjct: 83 KSRKLLFSLCGHMDYVRVC 101 >SPCC162.12 ||SPCC1753.06c|sequence orphan|Schizosaccharomyces pombe|chr 3|||Manual Length = 451 Score = 25.0 bits (52), Expect = 5.6 Identities = 12/36 (33%), Positives = 19/36 (52%) Frame = -2 Query: 301 TVRTDTSDEQMHSASLCMSFSKRRHSTTKSDALPSS 194 + T D+ + SASL S S R H+ +S +P + Sbjct: 396 SAETSKKDDGVQSASLNASMSARSHARQRSIHVPKT 431 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,823,623 Number of Sequences: 5004 Number of extensions: 33929 Number of successful extensions: 105 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 105 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 105 length of database: 2,362,478 effective HSP length: 67 effective length of database: 2,027,210 effective search space used: 174340060 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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