Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30419
(777 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g51190.1 68416.m05604 60S ribosomal protein L8 (RPL8B) riboso... 128 3e-30
At2g18020.1 68415.m02094 60S ribosomal protein L8 (RPL8A) 128 3e-30
At4g36130.1 68417.m05142 60S ribosomal protein L8 (RPL8C) riboso... 124 9e-29
At4g14250.1 68417.m02198 UBX domain-containing protein low simil... 46 4e-05
At2g44065.2 68415.m05480 ribosomal protein L2 family protein sim... 46 4e-05
At2g44065.1 68415.m05479 ribosomal protein L2 family protein sim... 46 4e-05
At5g49920.1 68418.m06181 octicosapeptide/Phox/Bem1p (PB1) domain... 32 0.49
At3g60860.1 68416.m06808 guanine nucleotide exchange family prot... 29 2.6
At4g33410.1 68417.m04748 signal peptide peptidase family protein... 29 3.4
At5g65290.1 68418.m08212 LMBR1 integral membrane family protein ... 28 6.0
At2g41230.1 68415.m05091 expressed protein 28 6.0
At1g22910.3 68414.m02863 RNA recognition motif (RRM)-containing ... 28 6.0
At4g03200.1 68417.m00437 expressed protein contains Pfam PF03190... 28 7.9
At4g00060.1 68417.m00006 nucleotidyltransferase family protein c... 28 7.9
>At3g51190.1 68416.m05604 60S ribosomal protein L8 (RPL8B) ribosomal
protein L8, cytosolic - Arabidopsis thaliana, PIR:T04582
Length = 260
Score = 128 bits (310), Expect = 3e-30
Identities = 54/83 (65%), Positives = 72/83 (86%)
Frame = +3
Query: 264 LYTGQFVYCGKKATLEVGNVMPVGAMPEGTIVCNLEEKMGDRGRLARASGNFATVIGHNP 443
+YTGQ++YCGKKA L VGNV+P+G++PEG ++CN+E +GDRG LARASG++A VI HNP
Sbjct: 83 MYTGQYLYCGKKANLMVGNVLPLGSIPEGAVICNVELHVGDRGALARASGDYAIVIAHNP 142
Query: 444 DAKRTRVKLPSGAKKVLPSSNRA 512
++ TRVKLPSG+KK+LPS+ RA
Sbjct: 143 ESNTTRVKLPSGSKKILPSACRA 165
Score = 118 bits (284), Expect = 4e-27
Identities = 49/60 (81%), Positives = 52/60 (86%)
Frame = +2
Query: 506 QGMVGIVAGGGRIDKPILKAGRAYHKYKVKRNCWPYVRGVAMNPVEHPHGGGNHQHIGKA 685
+ M+G VAGGGR +KP LKAG AYHKYK KRNCWP VRGVAMNPVEHPHGGGNHQHIG A
Sbjct: 164 RAMIGQVAGGGRTEKPFLKAGNAYHKYKAKRNCWPVVRGVAMNPVEHPHGGGNHQHIGHA 223
Score = 117 bits (282), Expect = 8e-27
Identities = 55/84 (65%), Positives = 66/84 (78%), Gaps = 1/84 (1%)
Frame = +2
Query: 23 MGRVIRAQRKGA-GSVFVSHTKKRKGAPKLRSLDYAERHGYIKGFVKDIIHDPGRGAPLA 199
MGRVIRAQRKGA GSVF SHT RKG K RSLDY ER+GY+KG V +IIHDPGRGAPLA
Sbjct: 1 MGRVIRAQRKGAAGSVFKSHTHHRKGPAKFRSLDYGERNGYLKGLVTEIIHDPGRGAPLA 60
Query: 200 VVHFRDPYKFKTRKELFIVPKALH 271
V FR P+++ +KELF+ + ++
Sbjct: 61 RVAFRHPFRYMKQKELFVAAEGMY 84
>At2g18020.1 68415.m02094 60S ribosomal protein L8 (RPL8A)
Length = 258
Score = 128 bits (310), Expect = 3e-30
Identities = 55/83 (66%), Positives = 71/83 (85%)
Frame = +3
Query: 264 LYTGQFVYCGKKATLEVGNVMPVGAMPEGTIVCNLEEKMGDRGRLARASGNFATVIGHNP 443
+YTGQF+YCGKKATL VGNV+P+ ++PEG +VCN+E +GDRG LARASG++A VI HNP
Sbjct: 82 MYTGQFLYCGKKATLVVGNVLPLRSIPEGAVVCNVEHHVGDRGVLARASGDYAIVIAHNP 141
Query: 444 DAKRTRVKLPSGAKKVLPSSNRA 512
D+ TR+KLPSG+KK++PS RA
Sbjct: 142 DSDTTRIKLPSGSKKIVPSGCRA 164
Score = 123 bits (297), Expect = 1e-28
Identities = 56/83 (67%), Positives = 67/83 (80%)
Frame = +2
Query: 23 MGRVIRAQRKGAGSVFVSHTKKRKGAPKLRSLDYAERHGYIKGFVKDIIHDPGRGAPLAV 202
MGRVIRAQRKGAGSVF SHT RKG K RSLD+ ER+GY+KG V +IIHDPGRGAPLA
Sbjct: 1 MGRVIRAQRKGAGSVFKSHTHHRKGPAKFRSLDFGERNGYLKGVVTEIIHDPGRGAPLAR 60
Query: 203 VHFRDPYKFKTRKELFIVPKALH 271
V FR P++FK +KELF+ + ++
Sbjct: 61 VTFRHPFRFKKQKELFVAAEGMY 83
Score = 114 bits (275), Expect = 5e-26
Identities = 48/60 (80%), Positives = 53/60 (88%)
Frame = +2
Query: 506 QGMVGIVAGGGRIDKPILKAGRAYHKYKVKRNCWPYVRGVAMNPVEHPHGGGNHQHIGKA 685
+ M+G VAGGGR +KP+LKAG AYHKY+VKRN WP VRGVAMNPVEHPHGGGNHQHIG A
Sbjct: 163 RAMIGQVAGGGRTEKPMLKAGNAYHKYRVKRNSWPKVRGVAMNPVEHPHGGGNHQHIGHA 222
>At4g36130.1 68417.m05142 60S ribosomal protein L8 (RPL8C) ribosomal
protein L8, cytosolic, tomato, PIR1:R5TOL8
Length = 258
Score = 124 bits (298), Expect = 9e-29
Identities = 52/83 (62%), Positives = 69/83 (83%)
Frame = +3
Query: 264 LYTGQFVYCGKKATLEVGNVMPVGAMPEGTIVCNLEEKMGDRGRLARASGNFATVIGHNP 443
+YTGQF+YCGKKATL VGNV+P+ ++PEG ++CN+E +GDRG ARASG++A VI HNP
Sbjct: 82 MYTGQFLYCGKKATLVVGNVLPLRSIPEGAVICNVEHHVGDRGVFARASGDYAIVIAHNP 141
Query: 444 DAKRTRVKLPSGAKKVLPSSNRA 512
D +R+KLPSG+KK++PS RA
Sbjct: 142 DNDTSRIKLPSGSKKIVPSGCRA 164
Score = 123 bits (297), Expect = 1e-28
Identities = 56/83 (67%), Positives = 67/83 (80%)
Frame = +2
Query: 23 MGRVIRAQRKGAGSVFVSHTKKRKGAPKLRSLDYAERHGYIKGFVKDIIHDPGRGAPLAV 202
MGRVIRAQRKGAGSVF SHT RKG K RSLD+ ER+GY+KG V +IIHDPGRGAPLA
Sbjct: 1 MGRVIRAQRKGAGSVFKSHTHHRKGPAKFRSLDFGERNGYLKGVVTEIIHDPGRGAPLAR 60
Query: 203 VHFRDPYKFKTRKELFIVPKALH 271
V FR P++FK +KELF+ + ++
Sbjct: 61 VAFRHPFRFKKQKELFVAAEGMY 83
Score = 118 bits (285), Expect = 3e-27
Identities = 49/60 (81%), Positives = 54/60 (90%)
Frame = +2
Query: 506 QGMVGIVAGGGRIDKPILKAGRAYHKYKVKRNCWPYVRGVAMNPVEHPHGGGNHQHIGKA 685
+ M+G VAGGGR +KP+LKAG AYHKY+VKRNCWP VRGVAMNPVEHPHGGGNHQHIG A
Sbjct: 163 RAMIGQVAGGGRTEKPMLKAGNAYHKYRVKRNCWPKVRGVAMNPVEHPHGGGNHQHIGHA 222
>At4g14250.1 68417.m02198 UBX domain-containing protein low
similarity to 60S ribosomal protein L2 [Nicotiana
tabacum] GI:9230281; contains Pfam profile PF00789: UBX
domain
Length = 724
Score = 45.6 bits (103), Expect = 4e-05
Identities = 20/27 (74%), Positives = 21/27 (77%), Gaps = 1/27 (3%)
Frame = +2
Query: 581 KYKVKRNCWPYVRGVAM-NPVEHPHGG 658
K +KRN W VRGVAM NPVEHPHGG
Sbjct: 411 KLMIKRNMWAKVRGVAMMNPVEHPHGG 437
>At2g44065.2 68415.m05480 ribosomal protein L2 family protein
similar to ribosomal protein L2 [Gossypium arboreum]
GI:17644114; contains Pfam profile PF03947: Ribosomal
Proteins L2, C-terminal domain
Length = 214
Score = 45.6 bits (103), Expect = 4e-05
Identities = 24/67 (35%), Positives = 38/67 (56%)
Frame = +3
Query: 312 VGNVMPVGAMPEGTIVCNLEEKMGDRGRLARASGNFATVIGHNPDAKRTRVKLPSGAKKV 491
+G+ MP+G M GTI+ N+E G ++ RA+G A ++ P + +KLPSG K
Sbjct: 59 IGSSMPLGMMRIGTIIHNIEMNPGQGAKMVRAAGTNAKIL-KEPAKGKCLIKLPSGDTKW 117
Query: 492 LPSSNRA 512
+ + RA
Sbjct: 118 INAKCRA 124
Score = 40.7 bits (91), Expect = 0.001
Identities = 18/30 (60%), Positives = 21/30 (70%), Gaps = 4/30 (13%)
Frame = +2
Query: 584 YKVKRNCW----PYVRGVAMNPVEHPHGGG 661
YK ++ W P VRGVAMNP +HPHGGG
Sbjct: 140 YKAGQSRWLGIRPKVRGVAMNPCDHPHGGG 169
>At2g44065.1 68415.m05479 ribosomal protein L2 family protein
similar to ribosomal protein L2 [Gossypium arboreum]
GI:17644114; contains Pfam profile PF03947: Ribosomal
Proteins L2, C-terminal domain
Length = 214
Score = 45.6 bits (103), Expect = 4e-05
Identities = 24/67 (35%), Positives = 38/67 (56%)
Frame = +3
Query: 312 VGNVMPVGAMPEGTIVCNLEEKMGDRGRLARASGNFATVIGHNPDAKRTRVKLPSGAKKV 491
+G+ MP+G M GTI+ N+E G ++ RA+G A ++ P + +KLPSG K
Sbjct: 59 IGSSMPLGMMRIGTIIHNIEMNPGQGAKMVRAAGTNAKIL-KEPAKGKCLIKLPSGDTKW 117
Query: 492 LPSSNRA 512
+ + RA
Sbjct: 118 INAKCRA 124
Score = 40.7 bits (91), Expect = 0.001
Identities = 18/30 (60%), Positives = 21/30 (70%), Gaps = 4/30 (13%)
Frame = +2
Query: 584 YKVKRNCW----PYVRGVAMNPVEHPHGGG 661
YK ++ W P VRGVAMNP +HPHGGG
Sbjct: 140 YKAGQSRWLGIRPKVRGVAMNPCDHPHGGG 169
>At5g49920.1 68418.m06181 octicosapeptide/Phox/Bem1p (PB1)
domain-containing protein contains Pfam profile
PF00564: PB1 domain
Length = 288
Score = 31.9 bits (69), Expect = 0.49
Identities = 23/54 (42%), Positives = 27/54 (50%), Gaps = 4/54 (7%)
Frame = -2
Query: 773 LALQKSFPVLRAAI--RPNFATSRCSSLDSGKPYLYVDGYHHR--EDALQGSWQ 624
L KS LRA I RP+F S CSS S P DGY H E + Q ++Q
Sbjct: 136 LRTSKSATALRAPIKTRPSFTVSTCSSPKSESP----DGYSHEPPETSFQNNYQ 185
>At3g60860.1 68416.m06808 guanine nucleotide exchange family protein
similar to guanine nucleotide exchange factor, Homo
sapiens, GI:5456754; contains Pfam profile PF01369: Sec7
domain
Length = 1793
Score = 29.5 bits (63), Expect = 2.6
Identities = 14/36 (38%), Positives = 22/36 (61%)
Frame = -3
Query: 766 SRNPFRSCEQQLGPILRPADVPLLTVESLTYMLMVT 659
S RS Q+LGP+ + D PLL +E+ +Y + +T
Sbjct: 1622 SNTILRSRLQELGPMTQMQDPPLLRLENESYQICLT 1657
>At4g33410.1 68417.m04748 signal peptide peptidase family protein
contains Pfam domain PF04258: Membrane protein of
unknown function (DUF435)
Length = 372
Score = 29.1 bits (62), Expect = 3.4
Identities = 16/44 (36%), Positives = 21/44 (47%), Gaps = 2/44 (4%)
Frame = -1
Query: 585 YLWYALPAFKIGL--SIRPPPATIPTMPCCLMAEPSWLQTVALL 460
Y+WYALP + IGL ++ T P L PS L V +
Sbjct: 301 YIWYALPGYAIGLVAALAAGVLTHSPQPALLYLVPSTLGPVIFM 344
>At5g65290.1 68418.m08212 LMBR1 integral membrane family protein
contains Pfam PF04791: LMBR1-like conserved region
Length = 733
Score = 28.3 bits (60), Expect = 6.0
Identities = 8/21 (38%), Positives = 13/21 (61%)
Frame = +1
Query: 529 WRWTY*QTYFESWKGIPQVQG 591
W W+Y T+ +W +P +QG
Sbjct: 74 WSWSYWSTFLLTWAVVPLIQG 94
>At2g41230.1 68415.m05091 expressed protein
Length = 88
Score = 28.3 bits (60), Expect = 6.0
Identities = 21/55 (38%), Positives = 27/55 (49%)
Frame = -1
Query: 660 PPP*GCSTGFMATPRTYGQQLRLTLYLWYALPAFKIGLSIRPPPATIPTMPCCLM 496
P P G + + T R+ L L+L L LP F L PPPAT+ +P LM
Sbjct: 14 PKPMGLNGSSLITARSVALLLFLSLLL-LILPPFLPPLP--PPPATLLLLPLLLM 65
>At1g22910.3 68414.m02863 RNA recognition motif (RRM)-containing
protein contains InterPro entry IPR000504: RNA-binding
region RNP-1 (RNA recognition motif) (RRM); similar to
GB:AAC33496
Length = 347
Score = 28.3 bits (60), Expect = 6.0
Identities = 23/81 (28%), Positives = 36/81 (44%), Gaps = 1/81 (1%)
Frame = -1
Query: 399 PDDLYHPFSLQDCTQWYPQA*-LQQASHFQLQELLSFHNKQIGLCRAFGTMKSSFLVLNL 223
P YH S + + P +QQ S +L + ++ + R FG+ ++S+ L L
Sbjct: 210 PQPFYHHLSSYNPHHYSPPTFSMQQGSRNRLSASTTSYSLPLKKLRVFGSYQASYHHLLL 269
Query: 222 YGSRKCTTAKGAPLPGSWIIS 160
SR KG SW+IS
Sbjct: 270 LRSRAILWFKGC----SWLIS 286
>At4g03200.1 68417.m00437 expressed protein contains Pfam PF03190:
Protein of unknown function, DUF255
Length = 818
Score = 27.9 bits (59), Expect = 7.9
Identities = 10/20 (50%), Positives = 11/20 (55%)
Frame = +2
Query: 656 GGNHQHIGKAFHCQERNICW 715
GG H HIG FH + CW
Sbjct: 329 GGMHDHIGGGFHRYSVDECW 348
>At4g00060.1 68417.m00006 nucleotidyltransferase family protein
contains Pfam profile: PF01909 nucleotidyltransferase
domain
Length = 839
Score = 27.9 bits (59), Expect = 7.9
Identities = 14/45 (31%), Positives = 28/45 (62%), Gaps = 1/45 (2%)
Frame = +2
Query: 71 VSHTKKRKGAPKLRSLDYAERHGYIKGFVKDIIH-DPGRGAPLAV 202
VS+++ R+G P +RS + RH ++G+ D ++ + RGA + +
Sbjct: 359 VSNSRARRGFPTVRSGSSSPRHWGMRGWFHDGVNWEEPRGAEIVL 403
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,840,110
Number of Sequences: 28952
Number of extensions: 430600
Number of successful extensions: 1142
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 1097
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1140
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1736283200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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