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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS30415
         (590 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g02590.1 68416.m00250 delta 7-sterol-C5-desaturase, putative ...    29   3.1  
At3g02580.1 68416.m00249 delta 7-sterol-C5-desaturase (STE1) ide...    28   4.1  
At2g19930.1 68415.m02329 RNA-dependent RNA polymerase family pro...    27   7.1  
At5g44390.1 68418.m05435 FAD-binding domain-containing protein s...    27   9.4  

>At3g02590.1 68416.m00250 delta 7-sterol-C5-desaturase, putative
           similar to delta7 sterol C-5 desaturase GI:5031219 from
           [Arabidopsis thaliana]
          Length = 279

 Score = 28.7 bits (61), Expect = 3.1
 Identities = 13/48 (27%), Positives = 24/48 (50%)
 Frame = +1

Query: 1   NRSKLKYIIF*SCWRRRPNFISTFILIFWTS*SLYFNFTRIWYNFSYY 144
           NR  L +++  + W   P+F+ T++  +     LYF    +W  + YY
Sbjct: 20  NRMVLSHLLPVNLWEPLPHFLQTWLRNYLAGNILYFISGFLWCFYIYY 67


>At3g02580.1 68416.m00249 delta 7-sterol-C5-desaturase (STE1)
           identical to sterol-C5-desaturase GB:AAD12944 GI:4234768
           from [Arabidopsis thaliana]
          Length = 281

 Score = 28.3 bits (60), Expect = 4.1
 Identities = 13/48 (27%), Positives = 24/48 (50%)
 Frame = +1

Query: 1   NRSKLKYIIF*SCWRRRPNFISTFILIFWTS*SLYFNFTRIWYNFSYY 144
           NR  L +++  + W   P+F+ T++  +     LYF    +W  + YY
Sbjct: 19  NRIVLSHLLPANLWEPLPHFLQTWLRNYLAGTLLYFISGFLWCFYIYY 66


>At2g19930.1 68415.m02329 RNA-dependent RNA polymerase family
           protein contains Pfam domain, PF05183: RNA dependent RNA
           polymerase
          Length = 977

 Score = 27.5 bits (58), Expect = 7.1
 Identities = 11/18 (61%), Positives = 12/18 (66%)
 Frame = -2

Query: 508 WEWAPTHKRYQCNVIPNG 455
           W+   TH  YQCNV PNG
Sbjct: 197 WDSGKTHY-YQCNVAPNG 213


>At5g44390.1 68418.m05435 FAD-binding domain-containing protein
           similar to SP|P30986 reticuline oxidase precursor
           (Berberine-bridge-forming enzyme) (BBE)
           (Tetrahydroprotoberberine synthase) [Eschscholzia
           californica]; contains PF01565 FAD binding domain
          Length = 542

 Score = 27.1 bits (57), Expect = 9.4
 Identities = 13/30 (43%), Positives = 16/30 (53%), Gaps = 3/30 (10%)
 Frame = -3

Query: 576 NKGDPVNKPPP---*LQILRPLGRAYGNGR 496
           N G P N PPP    LQ   P+G+ Y  G+
Sbjct: 356 NSGFPTNPPPPIEILLQAKSPIGKVYFKGK 385


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,138,245
Number of Sequences: 28952
Number of extensions: 225890
Number of successful extensions: 454
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 444
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 454
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1171109464
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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