Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30401
(702 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1002.03c |gls2||glucosidase II Gls2|Schizosaccharomyces pomb... 84 2e-17
SPAC30D11.01c ||SPAC56F8.01|alpha-glucosidase|Schizosaccharomyce... 45 1e-05
SPAPB24D3.10c |agl1|agl|alpha-glucosidase Agl1|Schizosaccharomyc... 44 2e-05
SPAC1039.11c ||SPAC922.02c|alpha-glucosidase|Schizosaccharomyces... 39 8e-04
SPCC16C4.15 |rml2||mitochondrial ribosomal protein subunit L2|Sc... 29 0.64
SPBC16A3.07c |nrm1||negative regulator of MBF|Schizosaccharomyce... 27 2.6
SPAC4G9.09c |arg11||N-acetyl-gamma-glutamyl-phosphate reductase/... 26 4.5
SPAC23H4.02 |ppk9||serine/threonine protein kinase Ppk9 |Schizos... 26 6.0
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 25 7.9
SPAC4F10.13c |mpd2||GYF domain|Schizosaccharomyces pombe|chr 1||... 25 7.9
>SPAC1002.03c |gls2||glucosidase II Gls2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 923
Score = 83.8 bits (198), Expect = 2e-17
Identities = 34/76 (44%), Positives = 49/76 (64%), Gaps = 1/76 (1%)
Frame = +1
Query: 1 LTAKGRKMVVIVDPHIKREPGYFLHEDATELGYYVKDRDGKD-YEGWCWPGSSSYPDFFN 177
L +K RK++VI+DPHIK +P YF+ ++ + Y VKD+ G D Y CWPG+S + DFFN
Sbjct: 438 LDSKSRKLIVILDPHIKNDPNYFVSKELIDYNYAVKDKSGVDNYNADCWPGNSVWVDFFN 497
Query: 178 PVVSRYYSDRYRFENF 225
P ++ Y F+ F
Sbjct: 498 PEAQAWWGSLYEFDRF 513
Score = 59.7 bits (138), Expect = 4e-10
Identities = 30/68 (44%), Positives = 37/68 (54%)
Frame = +3
Query: 255 NDMNEPSVFNGPEVTMPKDCRHYKPPQDGLEGLAAYWEHRHVHNEYGLWNLRATNTGLLD 434
NDMNEPSVF GPE +M +D HY WEHR +HN YG + T GL+
Sbjct: 523 NDMNEPSVFRGPETSMHRDAIHY-----------GGWEHRDIHNIYGHKCINGTYNGLIK 571
Query: 435 RADGSTDP 458
R +G+ P
Sbjct: 572 RGEGAVRP 579
Score = 39.1 bits (87), Expect = 6e-04
Identities = 16/36 (44%), Positives = 21/36 (58%)
Frame = +1
Query: 514 GDNTAEWSFLAASVPMCLSLAIAGNSFCGSDVGGFF 621
GD W L S+P L+ I+G +F G+DV GFF
Sbjct: 599 GDTMTTWEHLRGSIPTVLTNGISGMAFSGADVAGFF 634
Score = 27.5 bits (58), Expect = 2.0
Identities = 11/18 (61%), Positives = 14/18 (77%)
Frame = +2
Query: 452 RPFLLTRAVFAGTQRYSA 505
RPF+LTR+ FAGT +A
Sbjct: 578 RPFILTRSFFAGTSALAA 595
>SPAC30D11.01c ||SPAC56F8.01|alpha-glucosidase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 993
Score = 44.8 bits (101), Expect = 1e-05
Identities = 18/58 (31%), Positives = 30/58 (51%)
Frame = +1
Query: 25 VVIVDPHIKREPGYFLHEDATELGYYVKDRDGKDYEGWCWPGSSSYPDFFNPVVSRYY 198
+ +P+ + Y+ + E ++K+ +G Y G WPG YPDF NP V +Y+
Sbjct: 417 IYAANPNKSADRTYYPYYSGFEDNIFIKNPNGSAYVGMAWPGFVVYPDFTNPAVLQYW 474
Score = 31.1 bits (67), Expect = 0.16
Identities = 12/35 (34%), Positives = 21/35 (60%)
Frame = +1
Query: 514 GDNTAEWSFLAASVPMCLSLAIAGNSFCGSDVGGF 618
GDN ++WS + +S+ L+ + G G+DV G+
Sbjct: 671 GDNKSQWSDMVSSISSILTFNLLGIPMVGADVCGY 705
Score = 28.7 bits (61), Expect = 0.85
Identities = 11/18 (61%), Positives = 15/18 (83%)
Frame = +2
Query: 452 RPFLLTRAVFAGTQRYSA 505
RPFLL+R+ F G+ RY+A
Sbjct: 650 RPFLLSRSTFVGSGRYAA 667
>SPAPB24D3.10c |agl1|agl|alpha-glucosidase Agl1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 969
Score = 44.4 bits (100), Expect = 2e-05
Identities = 18/60 (30%), Positives = 32/60 (53%)
Frame = +1
Query: 25 VVIVDPHIKREPGYFLHEDATELGYYVKDRDGKDYEGWCWPGSSSYPDFFNPVVSRYYSD 204
+ +P+ + Y+ + E ++K+ +G Y G WPG +++PDF NP V Y+ D
Sbjct: 399 IYAANPYNHTDDSYYPYYAGVEKDIFLKNPNGSIYIGAVWPGFTAFPDFTNPDVVDYWKD 458
>SPAC1039.11c ||SPAC922.02c|alpha-glucosidase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 995
Score = 38.7 bits (86), Expect = 8e-04
Identities = 14/60 (23%), Positives = 32/60 (53%)
Frame = +1
Query: 25 VVIVDPHIKREPGYFLHEDATELGYYVKDRDGKDYEGWCWPGSSSYPDFFNPVVSRYYSD 204
+ +P + + Y+ + + ++++ D Y G WPG +++PDF NP + Y+++
Sbjct: 415 IYAANPINRSDDVYYPYYEGVRRDIFLRNPDRSLYVGNVWPGFTTFPDFTNPETTNYWTE 474
Score = 31.1 bits (67), Expect = 0.16
Identities = 14/35 (40%), Positives = 20/35 (57%)
Frame = +1
Query: 514 GDNTAEWSFLAASVPMCLSLAIAGNSFCGSDVGGF 618
GDN + WS + S+P L+ + G G+DV GF
Sbjct: 668 GDNYSLWSNMIFSIPGALTFNMVGLPMVGADVCGF 702
Score = 26.6 bits (56), Expect = 3.4
Identities = 9/18 (50%), Positives = 15/18 (83%)
Frame = +2
Query: 452 RPFLLTRAVFAGTQRYSA 505
RPF+L+R+ F G+ +Y+A
Sbjct: 647 RPFILSRSTFVGSGKYAA 664
>SPCC16C4.15 |rml2||mitochondrial ribosomal protein subunit
L2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 318
Score = 29.1 bits (62), Expect = 0.64
Identities = 19/71 (26%), Positives = 34/71 (47%), Gaps = 3/71 (4%)
Frame = -2
Query: 638 SFGDTLKNPPTSEPQKLFPAIASDKHIGTEAARNDHSAVLSP--VLP-QSISGCLRTRPS 468
SF LK EP ++ P +A++ GT AA+N P ++P ++ + P+
Sbjct: 128 SFRSLLKTGSNGEPVEMDPVLAAEIEDGTFAAKNLKPGNCFPLRLIPIGTVIHAIGVNPN 187
Query: 467 *AGRVCRPVGA 435
++CR G+
Sbjct: 188 QKAKLCRSAGS 198
>SPBC16A3.07c |nrm1||negative regulator of MBF|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 342
Score = 27.1 bits (57), Expect = 2.6
Identities = 16/45 (35%), Positives = 23/45 (51%), Gaps = 1/45 (2%)
Frame = -3
Query: 574 PVTSTSARKPQGTTTPPYCHLS-YRRVSLGACEHGPREQEGSVDP 443
PVTS ++ + PP HLS RR+S+G+ +Q G P
Sbjct: 176 PVTSIWNQRSLSSGKPPSLHLSGNRRLSMGSPTKSIYDQNGLTTP 220
>SPAC4G9.09c |arg11||N-acetyl-gamma-glutamyl-phosphate
reductase/acetylglutamate kinase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 885
Score = 26.2 bits (55), Expect = 4.5
Identities = 13/42 (30%), Positives = 23/42 (54%)
Frame = -3
Query: 571 VTSTSARKPQGTTTPPYCHLSYRRVSLGACEHGPREQEGSVD 446
++ S+R+ +GT P Y + V+L + E+EG+VD
Sbjct: 598 LSHVSSRELEGTKLPGYTKKEIQYVNLSTDDVKKLEEEGAVD 639
>SPAC23H4.02 |ppk9||serine/threonine protein kinase Ppk9
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 532
Score = 25.8 bits (54), Expect = 6.0
Identities = 24/65 (36%), Positives = 28/65 (43%), Gaps = 4/65 (6%)
Frame = -2
Query: 653 PRHELSFGDTLKNPPTSEPQK--LF-PAIAS-DKHIGTEAARNDHSAVLSPVLPQSISGC 486
P SF ++ S P LF P+I S +G DHSA LSP P SISG
Sbjct: 285 PSRTSSFSSKHRSVTFSPPDLAILFDPSITSPSSSVGQIPQPTDHSA-LSPSKPMSISGT 343
Query: 485 LRTRP 471
P
Sbjct: 344 ESPNP 348
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 25.4 bits (53), Expect = 7.9
Identities = 21/68 (30%), Positives = 29/68 (42%), Gaps = 3/68 (4%)
Frame = -2
Query: 695 GPGKNRLERGRLVPPRHELSFGDTLKNPPTSEPQKLF--PAIASDKHIGTEAARNDHSAV 522
GP +G ++P E S K P+ E QK+ P I ++ T A+ H AV
Sbjct: 561 GPNNEEETKGPVIPETQETSEQQVHKT-PSPEKQKVLSPPPIITNFDKETLASNEAHEAV 619
Query: 521 -LSPVLPQ 501
P PQ
Sbjct: 620 PQKPSAPQ 627
>SPAC4F10.13c |mpd2||GYF domain|Schizosaccharomyces pombe|chr
1|||Manual
Length = 992
Score = 25.4 bits (53), Expect = 7.9
Identities = 13/36 (36%), Positives = 22/36 (61%)
Frame = -2
Query: 515 PVLPQSISGCLRTRPS*AGRVCRPVGAVQQPRVGGA 408
PVL +S S ++R + +G + VG +QQP+ G+
Sbjct: 188 PVLDRSPSSFSQSRSAVSGNMNPGVGTLQQPQRAGS 223
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,804,529
Number of Sequences: 5004
Number of extensions: 55665
Number of successful extensions: 197
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 178
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 195
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 325165428
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -