Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30401
(702 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g63840.1 68418.m08014 alpha-glucosidase, putative similar to ... 121 4e-28
At5g11720.1 68418.m01369 alpha-glucosidase 1 (AGLU1) identical t... 53 2e-07
At3g23640.1 68416.m02973 glycosyl hydrolase family 31 protein si... 53 2e-07
At3g45940.1 68416.m04971 alpha-xylosidase, putative strong simil... 44 1e-04
At1g68560.1 68414.m07833 alpha-xylosidase (XYL1) identical to al... 42 4e-04
At4g18570.1 68417.m02749 proline-rich family protein common fami... 29 3.9
>At5g63840.1 68418.m08014 alpha-glucosidase, putative similar to
alpha-glucosidase GI:2648032 from [Solanum tuberosum]
Length = 921
Score = 121 bits (292), Expect = 4e-28
Identities = 46/83 (55%), Positives = 66/83 (79%)
Frame = +1
Query: 1 LTAKGRKMVVIVDPHIKREPGYFLHEDATELGYYVKDRDGKDYEGWCWPGSSSYPDFFNP 180
L AKGRKMV IVDPHIKR+ YFLH++AT++GYYVKD GKD++GWCWPGSSSY D +P
Sbjct: 426 LAAKGRKMVTIVDPHIKRDDSYFLHKEATQMGYYVKDSSGKDFDGWCWPGSSSYIDMLSP 485
Query: 181 VVSRYYSDRYRFENFPGTSKDVH 249
+ +++ R+ ++N+ G++ ++
Sbjct: 486 EIRKWWGGRFSYKNYVGSTPSLY 508
Score = 64.1 bits (149), Expect = 9e-11
Identities = 35/68 (51%), Positives = 42/68 (61%)
Frame = +3
Query: 255 NDMNEPSVFNGPEVTMPKDCRHYKPPQDGLEGLAAYWEHRHVHNEYGLWNLRATNTGLLD 434
NDMNEPSVFNGPEVTMP+D H + G+ EHR VHN YG + AT+ GL+
Sbjct: 511 NDMNEPSVFNGPEVTMPRDALH-------VGGV----EHREVHNAYGYYFHMATSDGLVM 559
Query: 435 RADGSTDP 458
R +G P
Sbjct: 560 REEGKDRP 567
Score = 55.6 bits (128), Expect = 3e-08
Identities = 22/37 (59%), Positives = 28/37 (75%)
Frame = +1
Query: 511 TGDNTAEWSFLAASVPMCLSLAIAGNSFCGSDVGGFF 621
TGDNTAEW L S+PM L+L + G +F G+D+GGFF
Sbjct: 586 TGDNTAEWEHLRVSIPMILTLGLTGITFSGADIGGFF 622
Score = 34.7 bits (76), Expect = 0.060
Identities = 13/19 (68%), Positives = 17/19 (89%)
Frame = +2
Query: 452 RPFLLTRAVFAGTQRYSAV 508
RPF+L+RA+F GTQRY A+
Sbjct: 566 RPFVLSRAIFPGTQRYGAI 584
>At5g11720.1 68418.m01369 alpha-glucosidase 1 (AGLU1) identical to
alpha-glucosidase 1 [Arabidopsis thaliana] GI:2323344
Length = 902
Score = 52.8 bits (121), Expect = 2e-07
Identities = 24/71 (33%), Positives = 39/71 (54%)
Frame = +1
Query: 1 LTAKGRKMVVIVDPHIKREPGYFLHEDATELGYYVKDRDGKDYEGWCWPGSSSYPDFFNP 180
L G+K V+I+DP I + Y + E ++K R+G+ Y G WPG +PDF NP
Sbjct: 376 LHKNGQKYVLILDPGIGVDSSYGTYNRGMEADVFIK-RNGEPYLGEVWPGKVYFPDFLNP 434
Query: 181 VVSRYYSDRYR 213
+ ++S+ +
Sbjct: 435 AAATFWSNEIK 445
Score = 37.5 bits (83), Expect = 0.009
Identities = 16/36 (44%), Positives = 22/36 (61%)
Frame = +1
Query: 511 TGDNTAEWSFLAASVPMCLSLAIAGNSFCGSDVGGF 618
TGDN A+W LA S+P L+ + G G+D+ GF
Sbjct: 556 TGDNAAKWEDLAYSIPGILNFGLFGIPMVGADICGF 591
>At3g23640.1 68416.m02973 glycosyl hydrolase family 31 protein
similar to alpha-glucosidase II from SP:Q9F234 [Bacillus
thermoamyloliquefaciens]; contains Pfam profile: PF01055
Glycosyl hydrolases family 31
Length = 991
Score = 52.8 bits (121), Expect = 2e-07
Identities = 20/68 (29%), Positives = 40/68 (58%)
Frame = +1
Query: 1 LTAKGRKMVVIVDPHIKREPGYFLHEDATELGYYVKDRDGKDYEGWCWPGSSSYPDFFNP 180
L + G K + ++DP IK+E GY++++ ++ ++ DGK + G WPG +PD+ N
Sbjct: 262 LHSNGFKAIWMLDPGIKQEEGYYVYDSGSKNDVWISRADGKPFTGEVWPGPCVFPDYTNS 321
Query: 181 VVSRYYSD 204
++++
Sbjct: 322 KARSWWAN 329
Score = 44.0 bits (99), Expect = 1e-04
Identities = 26/68 (38%), Positives = 36/68 (52%)
Frame = +3
Query: 255 NDMNEPSVFNGPEVTMPKDCRHYKPPQDGLEGLAAYWEHRHVHNEYGLWNLRATNTGLLD 434
NDMNEP+VF TMP++ H+ G + L H H HN YG+ R+T G ++
Sbjct: 344 NDMNEPAVFKVVTKTMPENNIHH-----GDDELGGVQNHSHYHNVYGMLMARSTYEG-ME 397
Query: 435 RADGSTDP 458
AD + P
Sbjct: 398 LADKNKRP 405
Score = 41.1 bits (92), Expect = 7e-04
Identities = 18/44 (40%), Positives = 25/44 (56%), Gaps = 1/44 (2%)
Frame = +1
Query: 511 TGDNTAEWSFLAASVPMCLSLAIAGNSFCGSDVGGFF-KVSPKL 639
TGDN + W L S+ M L L ++G G D+GGF +P+L
Sbjct: 424 TGDNLSNWEHLHMSISMVLQLGLSGQPLSGPDIGGFAGNATPRL 467
Score = 31.5 bits (68), Expect = 0.56
Identities = 13/18 (72%), Positives = 16/18 (88%)
Frame = +2
Query: 452 RPFLLTRAVFAGTQRYSA 505
RPF+LTRA F G+QRY+A
Sbjct: 404 RPFVLTRAGFIGSQRYAA 421
>At3g45940.1 68416.m04971 alpha-xylosidase, putative strong
similarity to alpha-xylosidase precursor GI:4163997 from
[Arabidopsis thaliana]
Length = 868
Score = 44.0 bits (99), Expect = 1e-04
Identities = 22/67 (32%), Positives = 32/67 (47%)
Frame = +1
Query: 13 GRKMVVIVDPHIKREPGYFLHEDATELGYYVKDRDGKDYEGWCWPGSSSYPDFFNPVVSR 192
G K VVI DP I Y +++ ++K +GK + WPG +PDF NP
Sbjct: 358 GMKYVVIKDPGIGVNASYGVYQRGMASDVFIK-YEGKPFLAQVWPGPVYFPDFLNPKTVS 416
Query: 193 YYSDRYR 213
++ D R
Sbjct: 417 WWGDEIR 423
Score = 38.3 bits (85), Expect = 0.005
Identities = 17/42 (40%), Positives = 22/42 (52%)
Frame = +1
Query: 511 TGDNTAEWSFLAASVPMCLSLAIAGNSFCGSDVGGFFKVSPK 636
TGDN W L S+ L+ I G GSD+ GFF +P+
Sbjct: 513 TGDNQGTWQSLQVSISTMLNFGIFGVPMVGSDICGFFPPTPE 554
>At1g68560.1 68414.m07833 alpha-xylosidase (XYL1) identical to
alpha-xylosidase precursor GB:AAD05539 GI:4163997 from
[Arabidopsis thaliana]; contains Pfam profile PF01055:
Glycosyl hydrolases family 31; identical to cDNA
alpha-xylosidase precursor (XYL1) partial cds GI:4163996
Length = 915
Score = 41.9 bits (94), Expect = 4e-04
Identities = 21/67 (31%), Positives = 31/67 (46%)
Frame = +1
Query: 13 GRKMVVIVDPHIKREPGYFLHEDATELGYYVKDRDGKDYEGWCWPGSSSYPDFFNPVVSR 192
G K +VI DP I Y + A ++K +GK + WPG +PDF NP
Sbjct: 361 GMKYIVINDPGIGVNASYGTFQRAMAADVFIK-YEGKPFLAQVWPGPVYFPDFLNPKTVS 419
Query: 193 YYSDRYR 213
++ D +
Sbjct: 420 WWGDEIK 426
Score = 34.7 bits (76), Expect = 0.060
Identities = 15/37 (40%), Positives = 19/37 (51%)
Frame = +1
Query: 511 TGDNTAEWSFLAASVPMCLSLAIAGNSFCGSDVGGFF 621
TGDN W L S+ L+ I G GSD+ GF+
Sbjct: 561 TGDNQGTWQSLQVSISTMLNFGIFGVPMVGSDICGFY 597
>At4g18570.1 68417.m02749 proline-rich family protein common family
members: At3g25690, At4g04980, At5g61090 [Arabidopsis
thaliana]
Length = 642
Score = 28.7 bits (61), Expect = 3.9
Identities = 12/35 (34%), Positives = 19/35 (54%)
Frame = -2
Query: 686 KNRLERGRLVPPRHELSFGDTLKNPPTSEPQKLFP 582
++R+ R PP+ +S GD+ +N PQK P
Sbjct: 276 RSRVPRVPKPPPKRSISLGDSTENRADPPPQKSIP 310
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,546,464
Number of Sequences: 28952
Number of extensions: 325284
Number of successful extensions: 925
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 876
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 922
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1506636208
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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