Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS30394
         (699 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC2C4.13 |vma16||V-type ATPase subunit c''|Schizosaccharomyces...   100   4e-22
SPAC1B3.14 |vma3||V-type ATPase subunit c|Schizosaccharomyces po...    52   1e-07
SPAC10F6.16 |mug134||endosulphine family protein|Schizosaccharom...    29   0.85 
SPBC2G2.02 |syj1||inositol-polyphosphate 5-phosphatase |Schizosa...    27   2.0  
SPAC23C4.19 |spt5||transcription elongation factor Spt5|Schizosa...    27   2.0  
SPBC354.05c |sre2||membrane-tethered transcription factor |Schiz...    27   3.4  
SPCC16C4.18c |taf50||histone H4-like TAF |Schizosaccharomyces po...    27   3.4  
SPBC16D10.05 |mok13||alpha-1,3-glucan synthase Mok13|Schizosacch...    26   4.5  
SPAC25H1.09 |mde5|meu30, SPAC4A8.01|alpha-amylase homolog Mde5|S...    26   6.0  
SPAC13F5.04c |||endosomal sorting protein |Schizosaccharomyces p...    25   7.9  
SPBC106.01 |mph1|SPBC1271.16c, SPBC243.01|dual specificity prote...    25   7.9  
SPAC1A6.09c |lag1||sphingosine N-acyltransferase Lag1|Schizosacc...    25   7.9  

>SPAC2C4.13 |vma16||V-type ATPase subunit c''|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 199

 Score = 99.5 bits (237), Expect = 4e-22
 Identities = 49/79 (62%), Positives = 55/79 (69%)
 Frame = +3

Query: 222 FGMVLGEHFPVMWGTLGIAFSVALSVVGAAMGIHTTGVSIVGGGVKAPRIKTKNLISVIF 401
           FG  L +  P  WG LGIA  VA  ++GAA GI   G SI+GG VKAPRIKTKNLIS+IF
Sbjct: 33  FGSFLLDTSPYTWGLLGIASCVAFGIIGAAWGIFICGTSILGGAVKAPRIKTKNLISIIF 92

Query: 402 CEAVAIYGLITAIVLSGML 458
           CE VAIY LI AIV S  +
Sbjct: 93  CEVVAIYSLIIAIVFSAKI 111


>SPAC1B3.14 |vma3||V-type ATPase subunit c|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 161

 Score = 51.6 bits (118), Expect = 1e-07
 Identities = 24/69 (34%), Positives = 39/69 (56%)
 Frame = +3

Query: 258 WGTLGIAFSVALSVVGAAMGIHTTGVSIVGGGVKAPRIKTKNLISVIFCEAVAIYGLITA 437
           +G +G   ++  +  GAA G    GV I   GV  P +  KN I V+    +AIYGL+ +
Sbjct: 13  FGVMGCTAAIVFASFGAAYGTAKAGVGISAMGVLRPDLIVKNTIPVVMAGIIAIYGLVVS 72

Query: 438 IVLSGMLEK 464
           +++SG L++
Sbjct: 73  VLISGNLKQ 81



 Score = 41.5 bits (93), Expect = 1e-04
 Identities = 21/61 (34%), Positives = 34/61 (55%)
 Frame = +3

Query: 267 LGIAFSVALSVVGAAMGIHTTGVSIVGGGVKAPRIKTKNLISVIFCEAVAIYGLITAIVL 446
           LG   SV L+ + A   I   G + V G  + PR+    ++ +IF E + +YGLI A++L
Sbjct: 92  LGAGLSVGLAGLAAGFAIGIVGDAGVRGTAQQPRLFVAMILILIFAEVLGLYGLIVALLL 151

Query: 447 S 449
           +
Sbjct: 152 N 152



 Score = 29.5 bits (63), Expect = 0.48
 Identities = 14/36 (38%), Positives = 22/36 (61%)
 Frame = +2

Query: 488 VRQAAELMAGYVMFGAGLAVGLVKSILWEFAVGYRG 595
           ++Q   L +G++  GAGL+VGL   +   FA+G  G
Sbjct: 79  LKQILSLYSGFIQLGAGLSVGLA-GLAAGFAIGIVG 113


>SPAC10F6.16 |mug134||endosulphine family
           protein|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 139

 Score = 28.7 bits (61), Expect = 0.85
 Identities = 26/99 (26%), Positives = 45/99 (45%), Gaps = 1/99 (1%)
 Frame = -2

Query: 635 RQRLAASVRAAPEPHDTQQQIPTE*ISPSQQRVQRRTSRIPPSVLLLDGHTSKWFRIFLQ 456
           R+ + ++   A    +++Q++    +SP +Q++ R   R+P    LL     K     LQ
Sbjct: 5   RKWMLSTTIIAMSSSNSEQKVDVAKLSPEEQKLFRLYGRLPQRKDLL---VQK-----LQ 56

Query: 455 HTREH-DSSDQTVNGDGLAEDDGDQILSLDSRCFDTSSH 342
             R++ DS D  +N  G A D G   +  +    DT  H
Sbjct: 57  QGRKYFDSGDYALNKAGKASDSGITCIGKEIPSPDTIPH 95


>SPBC2G2.02 |syj1||inositol-polyphosphate 5-phosphatase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1076

 Score = 27.5 bits (58), Expect = 2.0
 Identities = 16/58 (27%), Positives = 26/58 (44%)
 Frame = -3

Query: 631 SVWQHPLEQHQSPTIPNSKFPQNRFHQANSESSAEHHVSRHQFCCLTDTLVNGSEYFS 458
           S W H  +++ SP IP+        +  +++    +  +   F  L   L NGS YFS
Sbjct: 106 SNWDHIRQENYSPDIPDG-------YDTDTQGYDSYKYAAEPFSSLRKLLTNGSFYFS 156


>SPAC23C4.19 |spt5||transcription elongation factor
           Spt5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 990

 Score = 27.5 bits (58), Expect = 2.0
 Identities = 16/58 (27%), Positives = 28/58 (48%)
 Frame = -2

Query: 446 EHDSSDQTVNGDGLAEDDGDQILSLDSRCFDTSSHYADTSSVDAHGCSDNGQGNGKGD 273
           E D++   +N    A  +G  +L+ DS     +++   +S VD+H  S N     KG+
Sbjct: 22  EQDAASVKINSTR-ASPNGSDLLNDDSEAAKITTNEKQSSPVDSHNESPNDTTINKGE 78


>SPBC354.05c |sre2||membrane-tethered transcription factor
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 793

 Score = 26.6 bits (56), Expect = 3.4
 Identities = 21/79 (26%), Positives = 38/79 (48%), Gaps = 4/79 (5%)
 Frame = -3

Query: 646 LTNQGSVWQH---PLEQHQSPTIPNSKFPQNRFHQANS-ESSAEHHVSRHQFCCLTDTLV 479
           + +QG+ W+H    L+ +Q P +PN+KF      Q +S + S+ + V + +    T  L 
Sbjct: 171 INHQGNTWKHANESLQSNQGP-VPNTKFVGLETTQTDSYQQSSVNSVVKIEEADATYALR 229

Query: 478 NGSEYFSSIPESTIAVIKP 422
             S  F++   +    I P
Sbjct: 230 EESGQFNTFTGAENPSISP 248


>SPCC16C4.18c |taf50||histone H4-like TAF |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 452

 Score = 26.6 bits (56), Expect = 3.4
 Identities = 15/43 (34%), Positives = 23/43 (53%), Gaps = 1/43 (2%)
 Frame = +1

Query: 127 YFLSYLFVLLVGLAIPIF-SLYYVLNGKGEQISLGWFLENTSP 252
           Y L  L   L+G+    F ++YY L  +  + +L  FL+NT P
Sbjct: 302 YALRDLAAFLLGIVCDRFGNVYYTLKPRVTRTALKAFLDNTKP 344


>SPBC16D10.05 |mok13||alpha-1,3-glucan synthase
            Mok13|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 2358

 Score = 26.2 bits (55), Expect = 4.5
 Identities = 10/36 (27%), Positives = 23/36 (63%)
 Frame = +1

Query: 94   TKYLNQNLKMRYFLSYLFVLLVGLAIPIFSLYYVLN 201
            + ++N+N+ +R ++S++   +  +A    SLY+ LN
Sbjct: 2008 SSFINENMYLRLWISHIASWIYAVASASGSLYFSLN 2043


>SPAC25H1.09 |mde5|meu30, SPAC4A8.01|alpha-amylase homolog
           Mde5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 513

 Score = 25.8 bits (54), Expect = 6.0
 Identities = 10/32 (31%), Positives = 14/32 (43%)
 Frame = +3

Query: 165 CNPDIFAVLRPQWKG*ADKFGMVLGEHFPVMW 260
           CNP+        W+G  DK   + G  F  +W
Sbjct: 52  CNPEDREYCGGNWRGIIDKLDYIQGMGFTAIW 83


>SPAC13F5.04c |||endosomal sorting protein |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 277

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 11/26 (42%), Positives = 14/26 (53%)
 Frame = -1

Query: 573 SHRIDFTKPTASPAPNITYPAISSAA 496
           SH   F  PT SPA +++     SAA
Sbjct: 77  SHETSFALPTTSPAASLSISPTKSAA 102


>SPBC106.01 |mph1|SPBC1271.16c, SPBC243.01|dual specificity protein
           kinase Mph1 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 678

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 10/28 (35%), Positives = 16/28 (57%)
 Frame = -3

Query: 616 PLEQHQSPTIPNSKFPQNRFHQANSESS 533
           PL+ H +P   + + P+  FH +  ESS
Sbjct: 216 PLQLHNTPLQTSQEHPKPSFHPSQFESS 243


>SPAC1A6.09c |lag1||sphingosine N-acyltransferase
           Lag1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 390

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 12/30 (40%), Positives = 18/30 (60%)
 Frame = +3

Query: 411 VAIYGLITAIVLSGMLEKYSEPFTSVSVKQ 500
           ++I  +  A +LS  L  Y+EPF  +S KQ
Sbjct: 74  LSIIAICFACLLSPSLRPYAEPFIFLSYKQ 103


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,169,908
Number of Sequences: 5004
Number of extensions: 71261
Number of successful extensions: 250
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 242
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 250
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 323158234
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)