Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30389
(724 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g29210.1 68415.m03550 splicing factor PWI domain-containing p... 33 0.14
At1g09140.1 68414.m01018 SF2/ASF-like splicing modulator (SRP30)... 29 2.4
At4g23620.1 68417.m03402 50S ribosomal protein-related contains ... 29 3.1
At2g33793.1 68415.m04145 expressed protein 29 3.1
At5g11110.1 68418.m01297 sucrose-phosphate synthase, putative si... 29 4.1
At2g17410.1 68415.m02009 ARID/BRIGHT DNA-binding domain-containi... 29 4.1
At5g60500.1 68418.m07587 undecaprenyl pyrophosphate synthetase f... 28 5.5
At1g76810.1 68414.m08938 eukaryotic translation initiation facto... 28 5.5
At1g70620.1 68414.m08138 cyclin-related contains weak similarity... 28 5.5
At1g78400.1 68414.m09136 glycoside hydrolase family 28 protein /... 28 7.2
At1g62960.1 68414.m07109 1-aminocyclopropane-1-carboxylate synth... 28 7.2
At1g49980.1 68414.m05609 UMUC-like DNA repair family protein low... 27 9.5
>At2g29210.1 68415.m03550 splicing factor PWI domain-containing
protein contains Pfam profile PF01480: PWI domain
Length = 878
Score = 33.5 bits (73), Expect = 0.14
Identities = 43/171 (25%), Positives = 68/171 (39%), Gaps = 14/171 (8%)
Frame = +3
Query: 9 LSARIFYPLPHQKYIDSEWPAP*VTLPP*KT----SPRSPQT*RVSSKASTPAVSVTSTP 176
++ R+ P P + + S PA + PP + SP ++ +P+ S S P
Sbjct: 456 VAQRLPSPPPRRAGLPSPPPAQRLPSPPPRRAGLPSPMRIGGSHAANHLESPSPSSLSPP 515
Query: 177 MKRLCFRLLKTSPLRRPRSLYSTVSRV*F-------EPAEAHRDSGEEPASGQRRCRSGE 335
++ ++L + P+RR RSL RV P+ +D P G R +S
Sbjct: 516 GRK---KVLPSPPVRRRRSLTPDEERVSLSQGGRHTSPSHIKQDGSMSPVRG--RGKSSP 570
Query: 336 SPPEPLGRS*TLRQDSDEAHDDGRKESTA---PDRSYRSGEGKEQIPERHR 479
S RS R+ + R+ S+A PDR R + P R R
Sbjct: 571 SSRHQKARSPVRRRSPTPVNRRSRRSSSASRSPDRRRRRSPSSSRSPSRSR 621
>At1g09140.1 68414.m01018 SF2/ASF-like splicing modulator (SRP30)
nearly identical to SF2/ASF-like splicing modulator
Srp30 [Arabidopsis thaliana] GI:4775270
Length = 268
Score = 29.5 bits (63), Expect = 2.4
Identities = 22/71 (30%), Positives = 32/71 (45%), Gaps = 2/71 (2%)
Frame = +3
Query: 102 SPRSPQT*RVSSKASTPAVSVTSTPMKRLCFRLL--KTSPLRRPRSLYSTVSRV*FEPAE 275
SP ++ R S++ P+ S +S R + ++ PL R RSLYS+VSR
Sbjct: 193 SPDDSKSYRSRSRSRGPSCSYSSKSRSVSPARSISPRSRPLSRSRSLYSSVSRSQSRSKS 252
Query: 276 AHRDSGEEPAS 308
R P S
Sbjct: 253 RSRSRSNSPVS 263
>At4g23620.1 68417.m03402 50S ribosomal protein-related contains
weak similarity to 50S ribosomal protein L25 (TL5).
(Swiss-Prot:P56930) [Thermus thermophilus]
Length = 264
Score = 29.1 bits (62), Expect = 3.1
Identities = 14/35 (40%), Positives = 21/35 (60%), Gaps = 1/35 (2%)
Frame = +2
Query: 566 SLNHYFITVTRKC-ISLVSPYFNIDVSQIDLGRPL 667
SLN TV C ++ PY +D+SQ+D+G+ L
Sbjct: 188 SLNTIKRTVKFLCPAEIIPPYIEVDLSQLDIGQKL 222
>At2g33793.1 68415.m04145 expressed protein
Length = 212
Score = 29.1 bits (62), Expect = 3.1
Identities = 18/64 (28%), Positives = 32/64 (50%)
Frame = +3
Query: 369 LRQDSDEAHDDGRKESTAPDRSYRSGEGKEQIPERHRELRSH*AEAQETCEKNSLPTKDV 548
L+Q ++AH+DGRK+ S S E K +I E +L + K+S +++
Sbjct: 47 LQQFREKAHEDGRKKKEESISSV-STEVKSKIDELKSKLEKERQNFSKALSKSSKECENI 105
Query: 549 IEQE 560
++ E
Sbjct: 106 LKDE 109
>At5g11110.1 68418.m01297 sucrose-phosphate synthase, putative
similar to sucrose-phosphate synthase isoform 1, Citrus
unshiu, PIR:S72648
Length = 894
Score = 28.7 bits (61), Expect = 4.1
Identities = 23/74 (31%), Positives = 36/74 (48%)
Frame = +2
Query: 2 RVAECTNLLSPSSSKIY*FRMACSVSDTPSLKDLPKVATDLKSQLEGFNTSCLRDVDTNE 181
R+A C P ++ F + S S + SL+D+ ++ +LK L+G + VDTN
Sbjct: 513 RIASCKQR-HPKWQRVE-FENSDSDSPSDSLRDINDISLNLKLSLDGEKSGSNNGVDTN- 569
Query: 182 KIVLPSAEDVATEK 223
AED A E+
Sbjct: 570 ----LDAEDRAAER 579
>At2g17410.1 68415.m02009 ARID/BRIGHT DNA-binding domain-containing
protein contains Pfam profile PF01388: ARID/BRIGHT DNA
binding domain
Length = 786
Score = 28.7 bits (61), Expect = 4.1
Identities = 18/58 (31%), Positives = 28/58 (48%), Gaps = 3/58 (5%)
Frame = +2
Query: 71 SVSDTPSLKDLPKVATDLKSQLEGF---NTSCLRDVDTNEKIVLPSAEDVATEKTQKS 235
S+ + + DLPK+ + SQ E + S +DT E ++ P+AED E S
Sbjct: 78 SLEEVTNADDLPKIDDEKNSQFETSPHPSPSPSVALDTEEGLINPTAEDTVEENIVSS 135
>At5g60500.1 68418.m07587 undecaprenyl pyrophosphate synthetase
family protein / UPP synthetase family protein contains
putative undecaprenyl diphosphate synthase domain
[PF01255]; similar to S. cerevisiae dehydrodolichyl
diphosphate synthetase (DEDOL-PP
synthase)(Rer2)[SP|P35196], a cis-prenyltransferase
Length = 271
Score = 28.3 bits (60), Expect = 5.5
Identities = 14/40 (35%), Positives = 23/40 (57%)
Frame = -1
Query: 223 LLSGDVFSRRKHNLFIGVDVTETAGVEAFELTLQVCGDLG 104
++ G+ +KHNL IG+D AG + + LQ C ++G
Sbjct: 41 IMDGNRRFAKKHNL-IGLDAGHRAGFISVKYILQYCKEIG 79
>At1g76810.1 68414.m08938 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to IF2
protein [Drosophila melanogaster] GI:7108770; contains
Pfam profile PF03144: Elongation factor Tu domain 2
Length = 1294
Score = 28.3 bits (60), Expect = 5.5
Identities = 21/99 (21%), Positives = 45/99 (45%), Gaps = 5/99 (5%)
Frame = +3
Query: 282 RDSGEEPASGQRRCRSGESPPEPLGRS*TLRQDSDEAHDDGRKESTA----PDRSYRSGE 449
++ EE A+ +++ + E E + S E ++ ++ES P + G+
Sbjct: 344 KEGEEETAAAKKKKKKKEKEKEKKAAAAAAATSSVEVKEEKQEESVTEPLQPKKKDAKGK 403
Query: 450 GKEQ-IPERHRELRSH*AEAQETCEKNSLPTKDVIEQEK 563
E+ IP+ RE++ A QE E+ ++ + +E+
Sbjct: 404 AAEKKIPKHVREMQEALARRQEAEERKKKEEEEKLRKEE 442
>At1g70620.1 68414.m08138 cyclin-related contains weak similarity to
Swiss-Prot:P35662 cylicin I (Multiple-band polypeptide I)
[Bos taurus]
Length = 897
Score = 28.3 bits (60), Expect = 5.5
Identities = 20/83 (24%), Positives = 36/83 (43%)
Frame = +3
Query: 285 DSGEEPASGQRRCRSGESPPEPLGRS*TLRQDSDEAHDDGRKESTAPDRSYRSGEGKEQI 464
+S ++ R R SP R SDE+ DD +++S++ ++ GK
Sbjct: 794 ESSDDSKRKSRSRRRSVSPSPVRSRRKRSSPSSDESSDDSKRKSSSKRKNRSPSPGK--- 850
Query: 465 PERHRELRSH*AEAQETCEKNSL 533
R R + S ++ + KN+L
Sbjct: 851 -SRRRHVSSRSPHSKHSQHKNTL 872
>At1g78400.1 68414.m09136 glycoside hydrolase family 28 protein /
polygalacturonase (pectinase) family protein similar to
exopolygalacturonase GI:311962 from [Arabidopsis
thaliana]; contains Pfam profile PF00295: Glycosyl
hydrolases family 28 (polygalacturonases)
Length = 404
Score = 27.9 bits (59), Expect = 7.2
Identities = 9/35 (25%), Positives = 22/35 (62%)
Frame = +2
Query: 554 AREISLNHYFITVTRKCISLVSPYFNIDVSQIDLG 658
+ + ++H I CI+++S FN+D+++++ G
Sbjct: 212 SNHMKIHHVDIATGDDCIAILSGTFNLDINKVNCG 246
>At1g62960.1 68414.m07109 1-aminocyclopropane-1-carboxylate
synthase, putative / ACC synthase, putative similar to
GI:1173638 [GB:U35779] from [Triticum aestivum] (Plant
Mol. Biol. 31 (5), 1009-1020 (1996)), GI:1813331 from
Vigna radiata
Length = 557
Score = 27.9 bits (59), Expect = 7.2
Identities = 12/24 (50%), Positives = 15/24 (62%)
Frame = +2
Query: 650 DLGRPLQVLILGFCPNGKLSLGNW 721
+LG P V+ LG N KLSL +W
Sbjct: 162 ELGNPDGVIQLGLAQNNKLSLDDW 185
>At1g49980.1 68414.m05609 UMUC-like DNA repair family protein low
similarity to DNA polymerase kappa [Mus musculus]
GI:14279087; contains Pfam profile PF00817:
ImpB/MucB/SamB family
Length = 785
Score = 27.5 bits (58), Expect = 9.5
Identities = 12/42 (28%), Positives = 23/42 (54%)
Frame = -1
Query: 226 GLLSGDVFSRRKHNLFIGVDVTETAGVEAFELTLQVCGDLGE 101
GL G++ + +++ +T +AGV A L +VC D+ +
Sbjct: 214 GLSGGEIAEELRSSVYSETGLTCSAGVAANRLLAKVCSDINK 255
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,882,763
Number of Sequences: 28952
Number of extensions: 333787
Number of successful extensions: 1142
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 1078
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1142
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1575119672
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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